Abstract:
:Primary neuron cultures are widely used in research due to the ease and usefulness of observing individual cells. Therefore, it is vital to understand how variations in culture conditions may affect neuron physiology. One potential variation for cultured neurons is a change in intracellular transport. As transport is necessary for the normal delivery of organelles, proteins, nucleic acids, and lipids, it is a logical indicator of a cell's physiology. We test the hypothesis that organelle transport may change with varying in vitro population densities, thus indicating a change in cellular physiology. Using a novel background subtraction imaging method we show that, at 5 days in vitro (DIV), transport of vesicular organelles in embryonic rat spinal cord neurons is positively correlated with cell density. When density increased 6.5-fold, the number of transported organelles increased 2.2+/-0.3-fold. Intriguingly, this effect was not observable at 3-4 DIV. These results show a significant change in cellular physiology with a relatively small change in plating procedure; this indicates that cells appearing to be morphologically similar, and at the same DIV, may still suffer from a great degree of variability.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Bauer CT,Shtridelman Y,Lema Tomé CM,Grim JQ,Turner CP,Tytell M,Macosko JCdoi
10.1016/j.neulet.2008.06.026subject
Has Abstractpub_date
2008-08-22 00:00:00pages
173-7issue
2eissn
0304-3940issn
1872-7972pii
S0304-3940(08)00842-2journal_volume
441pub_type
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