Immunofluorescence analysis of the expression of Norpeg (Rai14) in retinal Müller and ganglion cells.

Abstract:

:Novel retinal pigment epithelial cell gene (Norpeg, Rai14), a developmentally regulated mouse gene, encodes a protein containing six ankyrin repeats and a coiled-coil domain. The objective of the present study was to characterize the Norpeg protein and to analyze its expression in mouse retina using an antibody preparation that we developed. The approximately 110kDa Norpeg was immunoprecipitated and characterized by mass spectrometry. Primary cultures of Müller and ganglion cells isolated from the mouse retina were found to express Norpeg when analyzed by immunoblotting and immunofluorescence microscopy. Immunofluorescence analysis of normal mouse retina sections demonstrated that Norpeg is expressed in cells of the ganglion cell layer, inner nuclear layer as well as in the retinal pigment epithelium. Immunoreactivity was also evident in the radial glial (Müller) cell fibers.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Kutty RK,Samuel W,Chen S,Vijayasarathy C,Dun Y,Mysona B,Wiggert B,Smith SB

doi

10.1016/j.neulet.2006.06.006

subject

Has Abstract

pub_date

2006-09-01 00:00:00

pages

294-8

issue

3

eissn

0304-3940

issn

1872-7972

pii

S0304-3940(06)00562-3

journal_volume

404

pub_type

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