Deoxyribonucleic acid repair and apoptosis in testicular germ cells of aging fertile men: the role of the poly(adenosine diphosphate-ribosyl)ation pathway.


OBJECTIVE:To explore the relationship between men's age and DNA damage repair proteins related to apoptosis in human testicular germ cells. DESIGN:Retrospective case-control study. SETTING:Academic institutions. PATIENT(S):Testicular specimens were obtained from 22 fertile volunteers aged 20-82 years. INTERVENTION(S):Deoxyribonucleic acid repair markers were assessed using immunohistochemical staining for the cell proliferation marker [proliferating cell nuclear antigen (PCNA)]; DNA repair markers [poly(adenosine diphosphate-ribose) polymerase-1 (PARP-1), poly(adenosine diphosphate-ribose) (PAR), X-ray repair cross-complementing1(XRCC1), and apurinic/apyrimidinic endonuclease 1 (APE1)]; and apoptosis-associated markers (caspase 9, active caspase 3, and cleaved PARP-1). MAIN OUTCOME MEASURE(S):The prevalence and cellular localization of the above markers in testicular tissues of young, middle aged, and old men. RESULT(S):Statistically significant differences in DNA damage repair-associated proteins (PARP-1, PAR, XRCC1, and APE1), and apoptosis markers (caspase 9, active caspase 3, and cleaved PARP-1) were observed in testicular samples from older men. These differences were most marked in spermatocytes. CONCLUSION(S):The study demonstrates that there is an age-related increase in human testicular germ cell DNA break repair and apoptosis with age.


Fertil Steril


Fertility and sterility


El-Domyati MM,Al-Din AB,Barakat MT,El-Fakahany HM,Xu J,Sakkas D




Has Abstract


2009-05-01 00:00:00




5 Suppl










  • Subcutaneous gonadotropin therapy in male patients with hypogonadotropic hypogonadism.

    abstract:OBJECTIVE:The response to subcutaneous (SC) gonadotropin replacement therapy, using human chorionic gonadotropin (hCG) and human menopausal gonadotropin (hMG) or hCG alone, was evaluated in male hypothalamic hypogonadism. DESIGN:Sixteen patients with hypothalamic hypogonadism were treated with gonadotropins for induct...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Saal W,Happ J,Cordes U,Baum RP,Schmidt M

    更新日期:1991-08-01 00:00:00

  • Preliminary studies on apoptosis in human fetal ovaries.

    abstract:OBJECTIVE:To evaluate if apoptosis occurs in human germ cells between 19 and 33 gestational weeks (GW). DESIGN:Human fetal ovaries were obtained from aborted fetuses aged 19-33 GW. SETTING:Rabin Medical Center, a major tertiary care and referral center. PATIENT(S):Twenty-seven women undergoing pregnancy termination....

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Abir R,Orvieto R,Dicker D,Zukerman Z,Barnett M,Fisch B

    更新日期:2002-08-01 00:00:00

  • Endometriosis may be associated with mitochondrial dysfunction in cumulus cells from subjects undergoing in vitro fertilization-intracytoplasmic sperm injection, as reflected by decreased adenosine triphosphate production.

    abstract:OBJECTIVE:To determine whether endometriosis is associated with mitochondrial dysfunction in cumulus (granulosa [GC]) cells of subjects undergoing IVF-intracytoplasmic sperm injection (ICSI). DESIGN:Prospective cohort study. SETTING:An IVF clinic in a tertiary academic care center. PATIENT(S):Eleven women with endom...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Hsu AL,Townsend PM,Oehninger S,Castora FJ

    更新日期:2015-02-01 00:00:00

  • A suboptimal endometrial pattern is associated with a reduced likelihood of pregnancy after a day 5 embryo transfer.

    abstract::This retrospective study examined the association of endometrial pattern and pregnancy after a day 5 ET. The pregnancy rate of women with a triple-line ultrasound endometrial pattern on the day of hCG administration was significantly higher than the pregnancy rate of women with the other ultrasound patterns. This obse...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Potlog-Nahari C,Catherino WH,McKeeby JL,Wesley R,Segars JH

    更新日期:2005-01-01 00:00:00

  • Report on the Third Annual Meeting of the Androgen Excess Society, San Diego, California, June 3, 2005.

    abstract::The third annual meeting of the Androgen Excess Society, held in San Diego, California, June 3, 2005, reported on new developments in the field, including intrafollicular steroidogenesis, intrathecal biochemistry, mechanisms of insulin signal and resistance of polycystic ovary syndrome, and novel aspects of androgen s...

    journal_title:Fertility and sterility



    authors: Orio F,Azziz R,Androgen Excess Society Annual Meeting Committee.,Androgen Excess Society.

    更新日期:2006-11-01 00:00:00

  • Clinical predictors for buserelin acetate treatment of uterine fibroids: a prospective study of 40 women.

    abstract::Buserelin acetate, a luteinizing hormone-releasing hormone agonist, is known to be effective in the shrinkage of uterine fibroids. A prospective trial was undertaken (1) to compare the efficacy of intranasal (IN) and subcutaneous (SC) administration of buserelin acetate and (2) to assess if tumor regression correlated...

    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Vollenhoven BJ,Shekleton P,McDonald J,Healy DL

    更新日期:1990-12-01 00:00:00

  • The frozen embryo and its nonresponding parents.

    abstract:OBJECTIVE:To describe patients who at least once did not reply to correspondence of the center about embryo disposition. DESIGN:Anonymous questionnaire. SETTING:Department of Reproductive Medicine, Ghent University Hospital (Belgium). PATIENT(S):Patients whose embryos were stored for at least 2 years. INTERVENTION(...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Provoost V,Pennings G,De Sutter P,Dhont M

    更新日期:2011-05-01 00:00:00

  • Luteal start vaginal micronized progesterone improves pregnancy success in women with recurrent pregnancy loss.

    abstract:OBJECTIVE:To assess the effectiveness of luteal start vaginal micronized P in a recurrent pregnancy loss (RPL) cohort. DESIGN:Observational cohort study using prospectively collected data. SETTING:Not applicable. PATIENT(S):Women seen between 2004 and 2012 with a history of two or more unexplained pregnancy losses <...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Stephenson MD,McQueen D,Winter M,Kliman HJ

    更新日期:2017-03-01 00:00:00

  • BRCA carriers have similar reproductive potential at baseline to noncarriers: comparisons in cancer and cancer-free cohorts undergoing fertility preservation.

    abstract:OBJECTIVE:To investigate whether BRCA carriers with and without malignancy have decreased ovarian reserve at baseline compared with BRCA noncarriers. DESIGN:Retrospective cohort study. SETTING:Academic medical center. PATIENT(S):Seven-hundred and ninety-five oocyte cryopreservation patients, comprising BRCA carriers...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Gunnala V,Fields J,Irani M,D'Angelo D,Xu K,Schattman G,Rosenwaks Z

    更新日期:2019-02-01 00:00:00

  • Repeated laparoscopic ovarian diathermy is effective in women with anovulatory infertility due to polycystic ovary syndrome.

    abstract:OBJECTIVE:To assess the effectiveness of a second laparoscopic ovarian diathermy (LOD) in women with polycystic ovary syndrome (PCOS). DESIGN:Retrospective study. SETTING:Tertiary infertility clinic. PATIENT(S):Twenty anovulatory infertile women with PCOS who underwent LOD 1-6 years previously. Twelve subjects had p...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Amer SA,Li TC,Cooke ID

    更新日期:2003-05-01 00:00:00

  • Assessment of insulin resistance in lean women with polycystic ovary syndrome.

    abstract:OBJECTIVE:To develop and validate a specific simple measure of insulin sensitivity using oral glucose tolerance test (OGTT) values for lean polycystic ovary syndrome (PCOS) women. DESIGN:Retrospective study. SETTING:Gynecologic Outpatient Clinic of University Hospital, affiliated with Unit of Gynecologic Endocrinolog...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Morciano A,Romani F,Sagnella F,Scarinci E,Palla C,Moro F,Tropea A,Policola C,Della Casa S,Guido M,Lanzone A,Apa R

    更新日期:2014-07-01 00:00:00

  • Ovarian and adipose tissue dysfunction in polycystic ovary syndrome: report of the 4th special scientific meeting of the Androgen Excess and PCOS Society.

    abstract::Significant advances have been made in our understanding of ovarian dysfunction in polycystic ovary syndrome (PCOS), and alterations in adipose tissue function are likely to play an important role in its pathophysiology. This review highlights the principal novel concepts presented at the 4th special scientific meetin...

    journal_title:Fertility and sterility

    pub_type: 共识发展会议,杂志文章,评审


    authors: Yildiz BO,Azziz R,Androgen Excess and PCOS Society.

    更新日期:2010-07-01 00:00:00

  • Liver function tests in women using intravaginal spermicide nonoxynol-9.

    abstract::The effects of intravaginal application of nonoxynol-9 (N-9) on hematological parameters, routine liver function biochemistry (bilirubin, serum bile acid, SGOT, SGPT, GGT, alkaline phosphatase), and serum lipids (cholesterol and triglyceride) were evaluated in 10 normal women who received 150 mg N-9 daily for 14 conse...

    journal_title:Fertility and sterility

    pub_type: 信件


    authors: Chvapil M,Droegemueller W,Earnest DL

    更新日期:1982-02-01 00:00:00

  • Ultrarapid freezing of early cleavage stage human embryos and eight-cell mouse embryos.

    abstract::Early cleavage stage human embryos and 8-cell mouse embryos were snap-frozen after a brief exposure to high concentrations of dimethyl sulfoxide (DMSO; 2 or 3.5 M) and 0.25 M sucrose and thawed in a warm water bath. Eleven of 12 3- to 8-cell human embryos survived freezing and thawing with more than 50% of their origi...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Trounson A,Peura A,Freemann L,Kirby C

    更新日期:1988-05-01 00:00:00

  • Presence of messenger ribonucleic acid for epidermal growth factor (EGF) and EGF receptor demonstrable in monolayer cell cultures of myometria and leiomyomata.

    abstract::There is a paucity of data concerning the factors involved in the growth of uterine leiomyomata. The expression of mRNAs encoding EGF and the EGF receptor in myometrial and leiomyoma cultures suggest that EGF may be involved in the autocrine/paracrine regulation of human uterine leiomyomata and myometrial growth. ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Yeh J,Rein M,Nowak R

    更新日期:1991-11-01 00:00:00

  • Assessing access to assisted reproductive services for serodiscordant couples with human immunodeficiency virus infection.

    abstract:OBJECTIVE:To understand the barriers that serodiscordant couples with human immunodeficiency virus (HIV) face in accessing services for risk reduction and infertility using assisted reproductive technology (ART). DESIGN:Two-arm cross-sectional telephone "secret shopper" study. SETTING:Infertility clinics designated b...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Leech AA,Bortoletto P,Christiansen C,Drainoni ML,Linas BP,Roeca C,Curtis M,Sullivan M

    更新日期:2018-03-01 00:00:00

  • Sperm deoxyribonucleic acid damage in normozoospermic men is related to age and sperm progressive motility.

    abstract:OBJECTIVE:To evaluate sperm DNA fragmentation in normozoospermic male partners of couples undergoing infertility evaluation. DESIGN:Retrospective cohort study. SETTING:Clinical andrology laboratory. PATIENT(S):A total of 1,974 consecutive normozoospermic men selected from a larger cohort of 4,345 consecutive, nonazo...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Belloc S,Benkhalifa M,Cohen-Bacrie M,Dalleac A,Amar E,Zini A

    更新日期:2014-06-01 00:00:00

  • Is "microscopic" peritoneal endometriosis invisible?

    abstract::This study fails to uphold the concept of invisible, microscopic endometriosis. Peritoneum devoid of disease exists, can be described, and can be consistently found. ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Redwine DB

    更新日期:1988-10-01 00:00:00

  • Hormonal contraception: recent advances and controversies.

    abstract::This document will outline new delivery systems and contraceptive formulations, summarize recent advances in emergency contraception, and review the effects of hormonal contraception on cancer risks, cardiovascular disease, and bone. ...

    journal_title:Fertility and sterility

    pub_type: 指南,杂志文章,实务指引


    authors: Practice Committee of the American Society for Reproductive Medicine.

    更新日期:2004-09-01 00:00:00

  • Subcutaneously administered Repronex in female patients undergoing in vitro fertilization is as effective and well tolerated as intramuscular menotropin treatment. Repronex SC, IVF Study Group.

    abstract:OBJECTIVE:To determine the efficacy and safety of Repronex SC as compared with Repronex IM and Pergonal IM in patients undergoing IVF. DESIGN:Open-label, randomized, parallel-group, multicenter study. SETTING:Fifteen academic and private fertility clinics with IVF experience. PATIENT(S):Premenopausal women with regu...

    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验


    authors: Gocial B,Keye WR,Fein SH,Nardi RV

    更新日期:2000-07-01 00:00:00

  • Embryo disposition: choices made by patients and donor oocyte recipients.

    abstract:OBJECTIVE:To compare final embryo disposition between patients and donor oocyte recipients. DESIGN:Retrospective study. SETTING:Private infertility practice. PATIENT(S):Patients undergoing IVF with embryo cryopreservation. INTERVENTION(S):None. MAIN OUTCOME MEASURE(S):Final cryopreserved embryo disposition. RESUL...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Hill GA,Freeman MR

    更新日期:2011-03-01 00:00:00

  • Leyomiomatosis peritonealis disseminata associated with ovarian endometriosis in a patient submitted to hysteroscopic myomectomy.

    abstract:OBJECTIVE:To describe laparoscopic management of a case of leyomiomatosis peritonealis disseminata associated with ovarian endometriosis. DESIGN:Surgical video article. SETTING:Academic hospital. PATIENT:We present a case of a 30-year-old woman referred to our clinic for abdominal and pelvic pain and dyspareunia. A ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Mabrouk M,Arena A,Raimondo D,Parisotto M,Caprara G,Seracchioli R

    更新日期:2019-06-01 00:00:00

  • Azoospermia after treatment with clomiphene citrate in patients with oligospermia.

    abstract:OBJECTIVE:To describe three cases of azoospermia in patients with oligospermia after clomiphene citrate (CC) intake. DESIGN:Case report. SETTING(S):Academic medical center. PATIENT(S):Three patients with oligospermia. INTERVENTION(S):Three oligospermic men used CC, resulting in azoospermia. MAIN OUTCOME MEASURE(S)...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Pasqualotto FF,Fonseca GP,Pasqualotto EB

    更新日期:2008-11-01 00:00:00

  • The different routes of administration and the effect of hormone replacement therapy on osteoporosis.

    abstract:OBJECTIVE:To review osteoporosis, a disease characterized by low bone mass, microarchitectural deterioration of bone tissue leading to increased bone fragility and a consequent increase in fracture risk. The disorder has become a major health problem in the West, where increased life expectancy has placed new emphasis ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,评审


    authors: Christiansen C

    更新日期:1994-12-01 00:00:00

  • A decade of experience emphasizes that testing for Y microdeletions is essential in American men with azoospermia and severe oligozoospermia.

    abstract:OBJECTIVE:To evaluate the benefit of Y microdeletion testing. DESIGN:Retrospective analysis. SETTING:University-based male fertility clinic and genetics laboratory. PATIENT(S):A total of 1,591 men with sperm concentrations less than 5 million sperm/mL. INTERVENTION(S):Semen analysis, Y microdeletion testing, microd...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Stahl PJ,Masson P,Mielnik A,Marean MB,Schlegel PN,Paduch DA

    更新日期:2010-10-01 00:00:00

  • Prevalence of hyperandrogenemia in the polycystic ovary syndrome diagnosed by the National Institutes of Health 1990 criteria.

    abstract:OBJECTIVE:To determine the prevalence of elevated total and free T, and DHEAS, alone and in combination, in patients with polycystic ovary syndrome (PCOS). DESIGN:Cross-sectional analysis. SETTING:Tertiary care academic medical center. PATIENT(S):Seven hundred twenty patients diagnosed with PCOS according to the Nat...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Huang A,Brennan K,Azziz R

    更新日期:2010-04-01 00:00:00

  • Pharmacological stimulation of sperm motility in frozen and thawed testicular sperm using the dimethylxanthine theophylline.

    abstract:OBJECTIVE:To evaluate whether the use of theophylline improves sperm motility and treatment outcome in frozen-thawed testicular sperm extraction (TESE). DESIGN:Artificial sperm activation was offered to azoospermic patients between January and October 2010 in two different centers (identical lab conditions). SETTING:...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Ebner T,Tews G,Mayer RB,Ziehr S,Arzt W,Costamoling W,Shebl O

    更新日期:2011-12-01 00:00:00

  • Ovarian pregnancy resulting from cornual fistulae in a woman who had undergone bilateral salpingectomy.

    abstract:OBJECTIVE:To report a case of ovarian pregnancy following in vitro fertilization-embryo transfer (IVF-ET) treatment for which the cornual fistulae was the most probable explanation of the cause. DESIGN:Case report. SETTING:University department and assisted reproduction unit. PATIENT(S):A 29-year-old woman with prim...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Hsu CC,Yang TT,Hsu CT

    更新日期:2005-01-01 00:00:00

  • Perinatal outcome of twin pregnancies after early transvaginal multifetal pregnancy reduction.

    abstract:OBJECTIVE:To compare the pregnancy outcomes of twin pregnancies following early transvaginal multifetal pregnancy reduction (MPR) with nonreduced twin gestations. DESIGN:Prospective cohort study. SETTING:Two tertiary medical centers. PATIENT(S):A cohort of 77 multiple pregnancies after reduction to twins and 78 dich...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,多中心研究


    authors: Haas J,Hourvitz A,Dor J,Elizur S,Yinon Y,Barzilay E,Shulman A

    更新日期:2014-05-01 00:00:00

  • Comparison of the efficacy of spironolactone versus flutamide in the treatment of hirsutism.

    abstract:OBJECTIVE:To compare the efficacy of two antiandrogens, spironolactone and flutamide, in the treatment of hirsutism. PATIENTS, PARTICIPANTS:Twenty women with idiopathic hirsutism were randomized to receive either flutamide or spironolactone. DESIGN:Twenty hirsute women were recruited from patients presenting to the h...

    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Erenus M,Gürbüz O,Durmuşoğlu F,Demirçay Z,Pekin S

    更新日期:1994-04-01 00:00:00