Deoxyribonucleic acid repair and apoptosis in testicular germ cells of aging fertile men: the role of the poly(adenosine diphosphate-ribosyl)ation pathway.

Abstract:

OBJECTIVE:To explore the relationship between men's age and DNA damage repair proteins related to apoptosis in human testicular germ cells. DESIGN:Retrospective case-control study. SETTING:Academic institutions. PATIENT(S):Testicular specimens were obtained from 22 fertile volunteers aged 20-82 years. INTERVENTION(S):Deoxyribonucleic acid repair markers were assessed using immunohistochemical staining for the cell proliferation marker [proliferating cell nuclear antigen (PCNA)]; DNA repair markers [poly(adenosine diphosphate-ribose) polymerase-1 (PARP-1), poly(adenosine diphosphate-ribose) (PAR), X-ray repair cross-complementing1(XRCC1), and apurinic/apyrimidinic endonuclease 1 (APE1)]; and apoptosis-associated markers (caspase 9, active caspase 3, and cleaved PARP-1). MAIN OUTCOME MEASURE(S):The prevalence and cellular localization of the above markers in testicular tissues of young, middle aged, and old men. RESULT(S):Statistically significant differences in DNA damage repair-associated proteins (PARP-1, PAR, XRCC1, and APE1), and apoptosis markers (caspase 9, active caspase 3, and cleaved PARP-1) were observed in testicular samples from older men. These differences were most marked in spermatocytes. CONCLUSION(S):The study demonstrates that there is an age-related increase in human testicular germ cell DNA break repair and apoptosis with age.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

El-Domyati MM,Al-Din AB,Barakat MT,El-Fakahany HM,Xu J,Sakkas D

doi

10.1016/j.fertnstert.2008.03.027

subject

Has Abstract

pub_date

2009-05-01 00:00:00

pages

2221-9

issue

5 Suppl

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(08)00598-0

journal_volume

91

pub_type

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