Abstract:
OBJECTIVE:To explore the relationship between men's age and DNA damage repair proteins related to apoptosis in human testicular germ cells. DESIGN:Retrospective case-control study. SETTING:Academic institutions. PATIENT(S):Testicular specimens were obtained from 22 fertile volunteers aged 20-82 years. INTERVENTION(S):Deoxyribonucleic acid repair markers were assessed using immunohistochemical staining for the cell proliferation marker [proliferating cell nuclear antigen (PCNA)]; DNA repair markers [poly(adenosine diphosphate-ribose) polymerase-1 (PARP-1), poly(adenosine diphosphate-ribose) (PAR), X-ray repair cross-complementing1(XRCC1), and apurinic/apyrimidinic endonuclease 1 (APE1)]; and apoptosis-associated markers (caspase 9, active caspase 3, and cleaved PARP-1). MAIN OUTCOME MEASURE(S):The prevalence and cellular localization of the above markers in testicular tissues of young, middle aged, and old men. RESULT(S):Statistically significant differences in DNA damage repair-associated proteins (PARP-1, PAR, XRCC1, and APE1), and apoptosis markers (caspase 9, active caspase 3, and cleaved PARP-1) were observed in testicular samples from older men. These differences were most marked in spermatocytes. CONCLUSION(S):The study demonstrates that there is an age-related increase in human testicular germ cell DNA break repair and apoptosis with age.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
El-Domyati MM,Al-Din AB,Barakat MT,El-Fakahany HM,Xu J,Sakkas Ddoi
10.1016/j.fertnstert.2008.03.027subject
Has Abstractpub_date
2009-05-01 00:00:00pages
2221-9issue
5 Suppleissn
0015-0282issn
1556-5653pii
S0015-0282(08)00598-0journal_volume
91pub_type
杂志文章abstract:OBJECTIVE:To create, validate, and use a fertility awareness survey based on current U.S. DATA: DESIGN:Cross-sectional study. SETTING:Not applicable. PATIENT(S):Phase 1 included U.S. women ages 18-45; phase 2 included female medical students and obstetrics and gynecology trainees at two urban academic programs. IN...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2017.07.1158
更新日期:2017-10-01 00:00:00
abstract::Cooling experiments of abdominal testes in adult, naturally cryptorchid pigs indicate that spermatogenic arrest in abdominal testes is not due to an inborn defect, but is caused solely by maintenance of the testis at abdominal temperature. It is postulated that failure of spermatogenic cells to differentiate after orc...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)44364-5
更新日期:1979-11-01 00:00:00
abstract:OBJECTIVE:To review the literature to determine the magnitude of the effect of 150 micrograms desogestrel-30 micrograms ethinyl E2, an oral contraceptive (OC) formulation, on plasma lipid concentrations in healthy women using meta-analysis techniques. DATA SOURCES:All English-language published reports (1981 to 1991) ...
journal_title:Fertility and sterility
pub_type: 杂志文章,meta分析
doi:
更新日期:1996-06-01 00:00:00
abstract:OBJECTIVE:To report on a one-year experience participating in a capitated healthcare plan for infertility. DESIGN:Prospective study. SETTING:University population. PATIENT(S):Reproductive-age women 15 to 50 years. INTERVENTION(S):The first-generation Lewin infertility algorithm and CATHI software were used to negot...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(01)01673-9
更新日期:2001-04-01 00:00:00
abstract:OBJECTIVE:To determine the prevalence of compound genetic abnormalities in patients who are carriers of cystic fibrosis mutations. DESIGN:Case report. SETTING:Tertiary referral center for male infertility. PATIENT(S):Between 2000 and 2005, 65 patients were identified to be carriers of cystic fibrosis transmembrane r...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2006.07.1537
更新日期:2007-06-01 00:00:00
abstract:OBJECTIVE:To assess the expression and cellular distribution of pregnancy-associated plasma protein-A (PAPP-A) and major basic protein (MBP) in human ovarian tissue during the menstrual cycle. DESIGN:Ovarian tissues (n = 50) and corpora lutea (n = 18) were obtained from patients undergoing hysterectomy/oophorectomy fo...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(03)00077-3
更新日期:2003-05-01 00:00:00
abstract:OBJECTIVE:To determine optimal management with one or two mature follicles after stimulation. DESIGN:Retrospective analysis. SETTING:Lister fertility clinic. PATIENT(S):A total of 1,350 IVF/intracytoplasmic sperm injection cycles (7.3% of total) during 1998-2009 were found to have one or two mature follicles. INTER...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.05.030
更新日期:2011-01-01 00:00:00
abstract::When dispersed spermatogenic cells obtained by enzymatic digestion from prepuberal mice, adult male mice, nonazoospermic men and normospermic men were observed live using Normarski optics, it was found that, respectively, 47.4%, 1.4%, 5.1%, and 2.4% of them protruded active pseudopodia. These cells were 8 to 10 mum in...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.06.055
更新日期:2008-08-01 00:00:00
abstract:OBJECTIVE:To identify whether the cause, site of ductal obstruction, and characteristics of fluid aspirates are associated with the cryosurvival and fertility after thawing of sperm obtained during reconstruction of the excurrent ducts with microsurgical epididymal sperm aspiration, vasal sperm aspiration, or both. DE...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(97)00274-4
更新日期:1997-10-01 00:00:00
abstract::The study and treatment of luteal phase defect since the late 1940s, particularly the work of Georgeanna Seegar Jones, are summarized. ...
journal_title:Fertility and sterility
pub_type: 传,历史文章,杂志文章
doi:10.1016/j.fertnstert.2007.08.036
更新日期:2008-11-01 00:00:00
abstract:OBJECTIVE:To evaluate the effects of tumor necrosis factor-alpha (TNF-alpha) on porcine oocyte maturation, spindle dynamics, and chromosome alignment. DESIGN:Controlled, prospective study. SETTING:University hospital and IVF research laboratory. ANIMAL(S):Ovaries collected from slaughtered prepubertal gilts. MAIN O...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2009.01.131
更新日期:2010-02-01 00:00:00
abstract::To evaluate the transfer of sperm from severely oligozoospermic men into the perivitelline space of mature oocytes, zona-drilling with acid phosphate-buffered saline (PBS) and direct zona-puncture in the presence of cytochalasin D were studied. Zona-drilling also was done for eggs from patients with previous failed in...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)60792-6
更新日期:1989-07-01 00:00:00
abstract::Peritoneal fluid and serum were collected from 78 patients at the time of laparoscopy. Twenty-two were fertile controls (CTL), and 56 were infertility patients, who were subdivided into three main groups: endometriosis (EMS), pelvic adhesions (ADH), and ovarian dysfunction (OvDF). Based on control group data, biochemi...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)48020-9
更新日期:1984-08-01 00:00:00
abstract::The number of live births from IVM oocytes has been increasing over the past two decades. Data concerning the health of IVM infants are reported by the authors of this Views and Reviews. ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2013.02.044
更新日期:2013-04-01 00:00:00
abstract:OBJECTIVE:To identify biomarkers associated with reproductive outcome using proton nuclear magnetic resonance ((1)H NMR) metabolomic profiling of embryo culture media. DESIGN:Retrospective study. SETTING:An academic assisted reproductive technology (ART) program; a university research center. PATIENT(S):Women underg...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2008.07.1739
更新日期:2008-12-01 00:00:00
abstract::Previous investigations have demonstrated the propensity of strong IgA-class sperm autoantibodies to impede fertilization. However, because there has not been a general consensus on this issue, the aim of this retrospective analysis was to focus on the effects of different levels of IgA-class antibodies on each stage ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2006.02.089
更新日期:2006-09-01 00:00:00
abstract::A battery-powered, bipolar electrocoagulator has been specifically developed for sealing the cut ends of the divided vas at vasectomy. With a minimum of electric power, the electrocoagulator destroys only the mucosa and one or two muscle cell layers of the vas, which leads to optimal fibrosis of the cut ends. This ins...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1978-06-01 00:00:00
abstract::Two hundred twenty consecutive in vitro fertilization (IVF) conception cycles were studied prospectively in order to examine the predictive value of serum human chorionic gonadotropin beta-subunit (beta-hCG), estradiol (E2), and progesterone (P) in predicting pregnancy outcome between 2 and 4 weeks after oocyte collec...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)60495-8
更新日期:1989-02-01 00:00:00
abstract:OBJECTIVE:To investigate endometrial dating during the conception cycle. DESIGN:Endometrial biopsies of the last half of the luteal phase of conception cycles were dated based on urinary luteinizing hormone (LH) surges. SETTING:Endometrial samples were obtained from women attending the fertility clinic of the Departm...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1992-12-01 00:00:00
abstract:OBJECTIVE:To compare the effects of serum with those of Albuminar-5 (Armour Pharmaceutical Co., Eastbourne, Sussex, United Kingdom) as medium supplements to Earl's balanced salt solution (EBSS) for IVF and subsequent embryo development. DESIGN:A retrospective study. Gametes and embryos from 318 patients were cultured ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)57978-3
更新日期:1995-12-01 00:00:00
abstract::After long-term estradiol deficiency, female rats displayed body mass gain accompanied by an increase in the size of adipocytes, an increase in hyperglycemia, and a decrease in insulinemia. The effects were reversed by daily estradiol treatment. Adiposity was suggested by the increased vascular endothelial growth fact...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2011.01.020
更新日期:2011-03-15 00:00:00
abstract:OBJECTIVE:To review the current understanding of the role the uterus plays in embryo implantation and to outline congenital anomalies and acquired diseases that impact normal uterine function. DESIGN:The publications related to the embryo implantation, Mullerian anomalies, uterine polyps, uterine synechiae, and myomas...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2007.09.069
更新日期:2008-01-01 00:00:00
abstract::It is suggested that heparin given in the peri-implantation period may improve clinical outcomes in women undergoing assisted reproduction techniques (ART). This systematic review evaluates the use of heparin in subfertile women undergoing ART. ...
journal_title:Fertility and sterility
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.fertnstert.2014.09.005
更新日期:2015-01-01 00:00:00
abstract::This study was initiated to determine whether factors are present in human sperm-free seminal plasma (HSP) that regulate the uptake and release of carnitine from sperm. Bovine caput epididymal sperm cells accumulated more carnitine than caudal sperm cells. A significant reduction in carnitine uptake by caput sperm was...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1988-05-01 00:00:00
abstract:OBJECTIVE:To describe different approaches for diagnosis and management of proximal ectopic pregnancies (PP) in general population. DESIGN:Observational population based-study. SETTING:Regional ectopic pregnancy registry. PATIENT(S):Eighty-six PP registered from 1992 to 2008. INTERVENTION(S):Surgical (radical or co...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.10.025
更新日期:2011-03-01 00:00:00
abstract:OBJECTIVE:To determine the distribution of catalase in eutopic and ectopic endometria in patients with endometriosis or adenomyosis. DESIGN:Retrospective randomized study. SETTING:Department of obstetrics and gynecology in a university hospital. PATIENT(S):Thirty-three patients with endometriosis, 36 with adenomyosi...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(02)03344-7
更新日期:2002-10-01 00:00:00
abstract:OBJECTIVE:To develop a consensus regarding the need for luteal phase support during assisted reproductive technology (ART), and to establish the optimal compound and route of administration for this purpose. DESIGN:Review of the published literature on luteal phase support. PATIENT(S):Women undergoing assisted reprod...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/s0015-0282(01)02961-2
更新日期:2002-02-01 00:00:00
abstract:OBJECTIVE:To determine the concentrations of angiopoietin 1 (ANGPT1) and ANGPT2 in individual human preovulatory follicles in relation to their diameter or volume to clarify the role of these molecules in folliculogenesis. DESIGN:Prospective study. SETTING:Research laboratory at Kansai Medical University, Osaka, Japa...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:10.1016/j.fertnstert.2011.09.031
更新日期:2011-12-01 00:00:00
abstract:OBJECTIVE:To describe the case of a Japanese woman with combined 17alpha-hydroxylase/17,20-lyase deficiency (congenital adrenal hyperplasia type V) and to discuss possible therapeutic procedures in such patients. DESIGN:Case report. SETTING:University hospital. PATIENT(S):A 26-year-old woman with secondary amenorrhe...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(00)00500-8
更新日期:2000-06-01 00:00:00
abstract:OBJECTIVE:To determine whether reactive oxygen species in peritoneal fluid might be a factor in infertility. DESIGN:Prospective study. SETTING:Andrology laboratory and gynecology clinic at a tertiary care facility. PATIENT(S):Women with endometriosis (n = 15) or idiopathic infertility (n = 11) who underwent laparosc...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(97)00343-9
更新日期:1997-11-01 00:00:00