Detection of apoptosis induced by new type gosling viral enteritis virus in vitro through fluorescein annexin V-FITC/PI double labeling.

Abstract:

AIM:To achieve a better understanding of the pathogenesis of new type gosling viral enteritis virus (NGVEV) and the relationship between NGVEV and host cells. METHODS:The apoptosis of duck embryo fibroblasts (DEF) induced by NGVEV was investigated by fluorescence-activated cell sorter (FACS) and fluorescence microscope after the cells were stained with Annexin V-FITC and propidium iodide (PI). RESULTS:By staining cells with a combination of fluorescein annexin V-FITC and PI, it is possible to distinguish and quantitatively analyze non-apoptotic cells (Annexin V-FITC negative/PI negative), early apoptotic cells (Annexin V-FITC positive/PI negative), late apoptotic/necrotic cells (Annexin V-FITC positive/PI positive) and dead cells (Annexin V-FITC negative/PI positive) through flow cytometry and fluorescence microscope. The percentage of apoptotic cells increased with the incubation time and reached a maximum at 120 h after infection, while the percentage of non-apoptotic cells decreased. CONCLUSION:NGVEV can induce the infected DEF cells to undergo apoptosis and the apoptosis occurs prior to necrosis.

journal_name

World J Gastroenterol

authors

Chen S,Cheng AC,Wang MS,Peng X

doi

10.3748/wjg.14.2174

subject

Has Abstract

pub_date

2008-04-14 00:00:00

pages

2174-8

issue

14

eissn

1007-9327

issn

2219-2840

journal_volume

14

pub_type

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