Abstract:
AIM:To achieve a better understanding of the pathogenesis of new type gosling viral enteritis virus (NGVEV) and the relationship between NGVEV and host cells. METHODS:The apoptosis of duck embryo fibroblasts (DEF) induced by NGVEV was investigated by fluorescence-activated cell sorter (FACS) and fluorescence microscope after the cells were stained with Annexin V-FITC and propidium iodide (PI). RESULTS:By staining cells with a combination of fluorescein annexin V-FITC and PI, it is possible to distinguish and quantitatively analyze non-apoptotic cells (Annexin V-FITC negative/PI negative), early apoptotic cells (Annexin V-FITC positive/PI negative), late apoptotic/necrotic cells (Annexin V-FITC positive/PI positive) and dead cells (Annexin V-FITC negative/PI positive) through flow cytometry and fluorescence microscope. The percentage of apoptotic cells increased with the incubation time and reached a maximum at 120 h after infection, while the percentage of non-apoptotic cells decreased. CONCLUSION:NGVEV can induce the infected DEF cells to undergo apoptosis and the apoptosis occurs prior to necrosis.
journal_name
World J Gastroenteroljournal_title
World journal of gastroenterologyauthors
Chen S,Cheng AC,Wang MS,Peng Xdoi
10.3748/wjg.14.2174subject
Has Abstractpub_date
2008-04-14 00:00:00pages
2174-8issue
14eissn
1007-9327issn
2219-2840journal_volume
14pub_type
杂志文章abstract::Pseudo-Meigs' syndrome associated with colorectal cancer is extremely rare. We report here a case of pseudo-Meigs' syndrome secondary to metachronous ovarian metastases from colon cancer. A 65-year-old female with a history of surgery for transverse colon cancer and peritoneal dissemination suffered from metachronous ...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
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journal_title:World journal of gastroenterology
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
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journal_title:World journal of gastroenterology
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pub_type: 杂志文章,meta分析,评审
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pub_type: 杂志文章
doi:10.3748/wjg.v8.i5.808
更新日期:2002-10-01 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v11.i34.5412
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journal_title:World journal of gastroenterology
pub_type: 社论
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pub_type: 杂志文章
doi:10.3748/wjg.v12.i44.7210
更新日期:2006-11-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v19.i20.3096
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pub_type: 杂志文章
doi:10.3748/wjg.v10.i20.2944
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pub_type: 杂志文章
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v16.i2.210
更新日期:2010-01-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
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更新日期:2007-04-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v25.i31.4534
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.14.4054
更新日期:2008-07-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v13.i23.3215
更新日期:2007-06-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v11.i31.4911
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pub_type: 杂志文章,meta分析,评审
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更新日期:2015-03-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v9.i6.1278
更新日期:2003-06-01 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.15.2552
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
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更新日期:2006-04-14 00:00:00