Isolated tumor cells and circulating CK20 mRNA in pN0 colorectal cancer patients.

Abstract:

:Micrometastases in lymph nodes and blood may provide important prognostic information. In this study, cytokeratin 20 (CK20) positive cells in lymph nodes and circulating CK20 mRNA were studied using 57 paraffin-embedded lymph node specimens and blood from 24 patients with pN0 colorectal cancer (CRC), respectively. Results showed that 29 out of 56 (52%) lymph node specimens had CK20-positive cells (range: 1-35). Follow-up of the patients for 12 months indicated that 4 patients (7%) had CRC metastases to liver, lung, and bone. In addition, 8 out of 24 (33%) samples had at least 2-fold circulating CK20 mRNA expression higher than the pooled normal sample. This study provides evidence that CK20-positive cells were found in the lymph nodes and differentially expressed circulating CK20 mRNA was also detected in the blood from patients with pN0 CRC. Long-term follow-up is necessary to study their prognostic use in patients with non-metastatic CRC.

journal_name

Int J Surg Pathol

authors

Wong CS,Cheung MT,Ma BB,Pun Hui E,Chan AC,Chan CK,Lee KC,Cheuk W,Lam MY,Wong MC,Chan CM,Chan JK,Chan AT

doi

10.1177/1066896907311901

subject

Has Abstract

pub_date

2008-04-01 00:00:00

pages

119-26

issue

2

eissn

1066-8969

issn

1940-2465

pii

1066896907311901

journal_volume

16

pub_type

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