Identification of mislabeled specimen by molecular methods: case report and review.

Abstract:

:Specimen misidentification is a common cause of errors in surgical pathology. We report a case where bone-marrow biopsies from patients of different genders were mislabeled and molecular methods were applied to resolve the identity. A short tandem repeat (STR)-polymerase chain reaction-based assay, commonly used in paternity testing, was employed in an attempt to assign the correct identity to the specimens. However, the specimens had been processed by decalcification and the DNA yield was poor. One of the markers in the assay is the non-STR amelogenin locus that distinguishes the X and Y chromosomes. This amelogenin marker results in a product of low molecular weight, enabling unequivocal resolution of identity despite a poor DNA yield. The prevalence of errors in pathology due to specimen misidentifications is reviewed.

journal_name

Int J Surg Pathol

authors

Mariappan MR,Zehnder J,Arber DA,Lay M,Fadare O,Schrijver I

doi

10.1177/106689690501300304

keywords:

subject

Has Abstract

pub_date

2005-07-01 00:00:00

pages

253-8

issue

3

eissn

1066-8969

issn

1940-2465

journal_volume

13

pub_type

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