Abstract:
:This study examines the occurrence, subtype and possible significance of haemopoietic inhibitory T cells (HIT cells) in patients with myelodysplasia (n = 21), acute myeloblastic leukaemia (n = 8) and in normal subjects (n = 13) using an autostimulary CFU-GM stem cell assay. HIT cells were detected when colony numbers increased by 30% or more following T-lymphocyte subpopulation ablation. HIT cells occurred in 28% of the MDS patients, were always CD8-positive and never occurred in the normal subjects. Sequential studies in MDS patients with HIT cells and treated with low dose Ara-C showed no correlation between occurrence of these cells and clinical outcome. HIT cells appear to be a clinically irrelevant manifestation of the cell dysfunction associated with MDS.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Smith MA,Smith JGdoi
10.1016/0145-2126(91)90028-rsubject
Has Abstractpub_date
1991-01-01 00:00:00pages
597-601issue
7eissn
0145-2126issn
1873-5835pii
0145-2126(91)90028-Rjournal_volume
15pub_type
杂志文章abstract::The efficiency of gene transfer into human leukemia cell lines by electroporation was investigated. For both transient expression (beta-galactosidase gene) and stable transformation (neomycin resistance gene), the transfer efficiency into leukemia cell lines using a square wave pulse was superior to that using an expo...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(91)90062-x
更新日期:1991-01-01 00:00:00
abstract::Using the human erythroleukaemic cell line K562 cl.6 and its daunorubicin-resistant subline K/DAU600, and the human T-lymphoblastic leukaemic cell line CCRF-CEM and its vinblastine-resistant subline CEM/VLB100, we have shown that the drug-resistant cell lines were more sensitive to cytotoxicity induced by tumour necro...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(94)00149-5
更新日期:1995-03-01 00:00:00
abstract::We evaluated the effect of SCF on myeloid differentiation by correlating clonogenic potential (as CFU-GM), bone marrow (BM) plasma SCF levels and CD34/c-kit expression in 57 MDS samples. There was a significant correlation between low SCF levels and 'leukemic' in vitro growth, the number of clusters and the colony/clu...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(98)00175-1
更新日期:1999-03-01 00:00:00
abstract::We report the characterization of a novel human T-cell line, HPB-MLp-W, which was established from blastic cells of a lymph node specimen from a patient with non-Hodgkin's lymphoma. They demonstrated the T-cell association antigens, CD2 and CD4, but no CD3, CD8, CD1, CD5, CD7 nor T-cell antigen receptor on their cell ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(91)90014-k
更新日期:1991-01-01 00:00:00
abstract::Cell line PER-423 was derived from the cells of a patient with an immature acute T-lymphoblastic leukaemia and the growth of this human cell line is strictly dependent on interleukin-2 (IL-2). PER-423 cells express the p75 (beta) subunit of the IL-2 receptor (IL-2R beta), while the p55 chain (IL-2R alpha) is not detec...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(93)90141-7
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND:Growing evidence demonstrates that an increased number of CD68 positive tumor-associated macrophages (TAM) is associated with decreased survival in patients with newly diagnosed classic Hodgkin lymphoma (HL). However, the impact of TAM in relapsed and refractory disease is unknown. DESIGN AND METHODS:To inv...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2013.03.021
更新日期:2013-09-01 00:00:00
abstract::This retrospective study of the thrombocythemia Italian registry (RIT) documented that 71 (30.6%) out of 232 ET patients experienced 88 cardiovascular adverse events (CV-AEs) during anagrelide treatment (522 pt-y). The rate of CV-AEs was: 24.1% for palpitations, 4.3% for angina, 3.5% for arterial hypertension, 3.0% fo...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2011.06.030
更新日期:2011-12-01 00:00:00
abstract::Human peripheral blood monocytes were found to be capable of phagocytizing human B-non Hodgkin's lymphoma (NHL) cells coated with mouse monoclonal antibodies (MoAbs). The MoAbs used recognized idiotypic determinants on the surface immunoglobulin (Ig) of the tumor cells. MoAbs of IgG1 and IgG2a subclass were equally ef...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(86)90344-9
更新日期:1986-01-01 00:00:00
abstract::Although verapamil, cyclosporin A. cremophor EL and PSC-833 are active as multidrug resistance modulators, there has been limited study of these compounds as possible chemotherapy enhancing agents against drug-sensitive tumors. We compared these agents as modifiers of VP-16 cytotoxicity in vitro and modifiers of VP-16...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(95)00029-n
更新日期:1995-08-01 00:00:00
abstract::In this study we investigated the Ara-CTP-forming capacity of leukemic cells in different phases of the cell cycle. Cells from two leukemic cell lines and leukemic bone marrow cells from patients and rats (BNML model) with acute myelocytic leukemia were separated according to cell cycle phase by means of an albumin de...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(90)90164-5
更新日期:1990-01-01 00:00:00
abstract::Model cell lines are essential tools for investigating the biology and therapeutics of cancer. Approximately 1500 human hematopoietic neoplastic cell lines have been described, covering most major disease entities. Waldenström's macroglobulinemia (WM) is a rare incurable hematological neoplasm from which only three ce...
journal_title:Leukemia research
pub_type: 杂志文章,评审
doi:10.1016/j.leukres.2008.04.016
更新日期:2008-11-01 00:00:00
abstract::We have utilized the MTT assay to measure the metabolic activity of cells from the bone marrow of 55 patients with acute myeloid leukaemia (AML), myelodysplastic syndrome (MDS) and non-clonal disease. Doubling dilutions of cells were exposed to MTT for 3-4 h. The mean optical density of the formazan produced by each c...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(95)00163-8
更新日期:1996-05-01 00:00:00
abstract::We studied differentiation inducing effects of retinoic acid (RA), 1 alpha, 25-dihydroxyvitamin D3 (D3) and interferons (IFNs), alone and in combination, on fresh myeloid leukemic cells from 8 patients. RA not only induced the differentiation of leukemic cells in 5/8 cases, but potentiated differentiation by IFNs eith...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(90)90072-h
更新日期:1990-01-01 00:00:00
abstract::We have previously shown that HL-60 cells exposed to all-trans retinoic acid (ATRA) after treatment with a non-cytotoxic concentration of vincristine (VCR) result in granulocytic maturation and differentiation, suggesting that VCR might exhibit a synergistic action with ATRA in the treatment of acute promyelocytic leu...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(96)00090-2
更新日期:1997-01-01 00:00:00
abstract::This study was conducted to determine the frequency of the most common fusion genes in Mexican pediatric patients with acute lymphoblastic leukemia (ALL). Molecular analysis using RT-PCR was carried out in 53-blood samples: 52 patients with de novo ALL and one with relapsed ALL. The ETV6-RUNX1 fusion was found in 7 ca...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2008.03.021
更新日期:2008-10-01 00:00:00
abstract::Acute promyelocytic leukemia (APL) is highly malignant and frequently expresses the PML-RARalpha (promyelocytic leukemia-retinoic acid receptor-alpha) fusion protein. This fusion protein is targeted by all-trans retinoic acid (ATRA) and arsenic trioxide (As2O3), presently used in APL therapy. We have evaluated effects...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2007.06.021
更新日期:2008-02-01 00:00:00
abstract::This study examined the immunoregulatory role of recombinant interleukin 4 (IL-4), also known as B-cell stimulating factor 1, on the generation of cytotoxic effector cells from normal and leukaemic human blood mononuclear cells. When tested on cells from normal individuals, the addition of IL-4 to mixed lymphocyte cul...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(89)90066-0
更新日期:1989-01-01 00:00:00
abstract::Pycnogenol, rich of many phytochemicals of medical value, is a commercialized nutrient supplement extracted from the bark of European coastal pine. In this study, we investigated the anti-tumor effects of Pycnogenol on HL-60, U937 and K562 human leukemia cell lines. We found that Pycnogenol inhibited cell proliferatio...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2004.10.006
更新日期:2005-06-01 00:00:00
abstract::Chronic myelogenous leukemia (CML) and 25% of adult onset acute lymphoblastic leukemia (ALL) are associated with the expression of Bcr-Abl, a constitutively activated protein tyrosine kinase. Bcr-Abl associated leukemias are characterized by a high degree of chromosomal and genomic instability. It is unclear if the ph...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(01)00181-3
更新日期:2002-11-01 00:00:00
abstract::Imatinib is a tyrosine kinase inhibitor and is now used regularly in chronic myeloid leukaemia therapy in chronic phase with great success. This drug due its very nature of action is suspected to be teratogenic hence the patients are counseled not to get pregnant while on this drug. However in world literature few nor...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2008.03.007
更新日期:2008-10-01 00:00:00
abstract::c-Myc has been implicated in a variety of hematologic malignancies including Burkitt's lymphoma. Targeting c-Myc driven growth pathways could be therapeutically useful but might require the identification of critical downstream proteins. Here we show that the serine-threonine kinase PBK/TOPK is frequently overexpresse...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2012.11.010
更新日期:2013-04-01 00:00:00
abstract::Chronic myeloid leukaemia (CML) patients in chronic phase (CP) are currently treated with a standard dose of imatinib of 400mg/daily. However, once in complete cytogenetic remission (CCR) it is possible that some patients maintain this status with reduced dose of the drug. Here, we describe five cases of CML in late C...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2008.10.024
更新日期:2009-05-01 00:00:00
abstract::To determine the clinical significance of the T helper 1(Th1)/T helper 2 (Th2) ratio in multiple myeloma, the intracellular IFN-gamma and IL-4 were analyzed by flow cytometry in 56 patients with multiple myeloma. The mean Th1/Th2 ratio of cases in the initial diagnosis phase and the refractory phase were higher than t...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2004.06.003
更新日期:2005-02-01 00:00:00
abstract::Acute myeloid leukemia (AML) is a heterogeneous disease. Until recently, treatment for patients with AML was limited to induction chemotherapy with cytarabine and anthracycline or hypomethylating agents, and, in some instances, allogeneic hematopoietic stem cell transplant. With the recent approval of new therapies-i....
journal_title:Leukemia research
pub_type: 杂志文章,评审
doi:10.1016/j.leukres.2018.09.001
更新日期:2018-10-01 00:00:00
abstract::As a part of our continuing efforts to develop gene therapy for acute myelogenous leukemia (AML), this study was undertaken to evaluate the possibility of using autologous bone marrow stromal fibroblasts (BMSFs) as a target cell population. Autologous BMSFs in AML were isolated from the stromal layers of long-term bon...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(01)00134-5
更新日期:2002-04-01 00:00:00
abstract::We report a novel chromosomal translocation in AML, t(X;21)(q25-26;q22), resulting in a fusion transcript between two ETS domain family members, ELF4 (at Xq25) and ERG (at 21q22). ERG has been associated previously with other fusion partners, specifically FUS and EWSR1, and implicated in both AML and Ewing's sarcoma. ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2005.10.014
更新日期:2006-08-01 00:00:00
abstract:BACKGROUND:Current results regarding treatment outcomes in acute myeloid leukemia (AML) point to significant differences between low- and middle-income countries (LMIC) and high-income countries (HIC). Excluding well-known socioeconomic issues, genetic markers important for prognosis have not been properly incorporated...
journal_title:Leukemia research
pub_type: 杂志文章,多中心研究
doi:10.1016/j.leukres.2017.07.005
更新日期:2017-09-01 00:00:00
abstract::AKT1 is the human homolog of the v-akt oncogene. AKT1 has two distinct protein domains, one serine/threonine kinase domain and one pleckstrin homology (PH) domain. We studied the expression and activity of AKT1 in hematopoietic cell lines. The expression of AKT1 was constitutive in hematopoietic cells of various stage...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(97)00055-6
更新日期:1997-09-01 00:00:00
abstract::DNAs from chronic granulocytic leukaemia (CGL) and chronic myelomonocytic leukaemia (CMML) were assayed for transforming genes by transfection into NIH 3T3 cells. Foci DNA was tagged with a geneticin-resistance cosmid, then followed through a drug selection and tumorigenicity assay. Activated Ha-ras genes, with point ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(88)90033-1
更新日期:1988-01-01 00:00:00
abstract::We investigated the therapeutic activity of recombinant erythropoietin (r-EPO) in association with thalidomide in 30 patients with myelodysplastic syndromes (MDS), previously treated with r-EPO (n.15, group A) or thalidomide (n.15, group B) as single agents, respectively, without any significant benefit on their anemi...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2005.08.020
更新日期:2006-04-01 00:00:00