Characterization and partial purification of Candida albicans Secretory IL-12 Inhibitory Factor.

Abstract:

BACKGROUND:We have previously shown that supernatant from Candida albicans (CA) culture contains a Secretory Interleukin (IL)-12 Inhibitory Factor (CA-SIIF), which inhibits IL-12 production by human monocytes. However, the effect of CA-SIIF on secretion of other cytokines by monocytes is unknown, and detailed characterization of this factor has not been performed. RESULTS:In this study, we demonstrate that the IL-12 inhibitory activity of CA-SIIF was serum-independent, based on the reduction of IL-12 levels in monocytes stimulated under serum-independent conditions. The minimal inhibitory dose of CA-SIIF was found to be 200 mug/ml. Investigation of CA-SIIF's effect on macrophages IL-12 production in vitro and in vivo also showed that CA-SIIF inhibited IL-12 production by murine macrophages both in vitro (from 571 +/- 24 pg/ml to 387 +/- 87 pg/ml; P = 0.05) and in vivo (from 262 +/- 6 pg/ml to 144 +/- 30 pg/ml; P < 0.05). In addition to IL-12, cytokine array analysis revealed that CA-SIIF induced differential production of other cytokines also. In this regard, reduction in levels were observed for IL-8, IL-10, IL-13, monocyte chemoattractant protein (MCP)-1, MCP-2, macrophage inflammatory protein (MIP)-1, RANTES, etc. In contrast, levels of other chemokines e.g. MCP-4, MIF and MIP-3alpha (P < 0.05) were increased. We also found that CA-SIIF suppressed the maturation of human monocytes to dendritic cells (CD1a expression = 13 +/- 3% vs 36 +/- 2% of the control; P < 0.01). Next, to identify the biochemical nature of CA-SIIF, we separated this factor into a Concanavalin A (ConA)-binding glycoprotein fraction (CA-SIIF-GP) and a non-ConA-binding protein fraction (CA-SIIF-NGP) using ConA affinity chromatography. Both fractions were then tested for this inhibitory effect on human monocyte IL-12 production. CA-SIIF-GP produced a higher inhibitory effect on IL-12 production compared to CA-SIIF-NGP and CA-SIIF crude (P < 0.01), proving that CA-SIIF is a glycoprotein in nature. CONCLUSION:CA-SIIF is a glycoprotein which exhibits serum-independent inhibition of IL-12 production from monocytes in vitro and in vivo, and also modulates differentiation of monocytes into dendritic cells. These results suggest important role for CA-SIIF in interactions of C. albicans with the host immune system.

journal_name

BMC Microbiol

journal_title

BMC microbiology

authors

Wang M,Mukherjee PK,Chandra J,Lattif AA,McCormick TS,Ghannoum MA

doi

10.1186/1471-2180-8-31

subject

Has Abstract

pub_date

2008-02-19 00:00:00

pages

31

issn

1471-2180

pii

1471-2180-8-31

journal_volume

8

pub_type

杂志文章
  • Genetic analysis of enteropathogenic Escherichia coli (EPEC) adherence factor (EAF) plasmid reveals a new deletion within the EAF probe sequence among O119 typical EPEC strains.

    abstract:BACKGROUND:Enteropathogenic Escherichia coli (EPEC) are classified into typical and atypical strains based on the presence of the E. coli adherence factor (EAF) plasmid. The EAF plasmid contains the bfp (bundle-forming pilus) operon and the perABC (plasmid encoded regulator) gene cluster. A 1-kb cryptic region of EAF p...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0539-9

    authors: Teixeira NB,Rojas TC,da Silveira WD,Matheus-Guimarães C,Silva NP,Scaletsky IC

    更新日期:2015-10-05 00:00:00

  • Secular trends of blood isolates in patients from a rural area population hospitalized in a tertiary center in a small city in Greece.

    abstract:BACKGROUND:Most of the studies evaluating the secular trends of blood isolates come from tertiary hospitals in urban areas. We sought to study the trends of the antimicrobial resistance of blood isolates in patients from a rural population hospitalized in a tertiary hospital in a small city in Greece. METHODS:We retro...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-6-41

    authors: Falagas ME,Bakossi A,Pappas VD,Holevas PV,Bouras A,Stamata E

    更新日期:2006-05-02 00:00:00

  • Longitudinal survey of Clostridium difficile presence and gut microbiota composition in a Belgian nursing home.

    abstract:BACKGROUND:Increasing age, several co-morbidities, environmental contamination, antibiotic exposure and other intestinal perturbations appear to be the greatest risk factors for C. difficile infection (CDI). Therefore, elderly care home residents are considered particularly vulnerable to the infection. The main objecti...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0848-7

    authors: Rodriguez C,Taminiau B,Korsak N,Avesani V,Van Broeck J,Brach P,Delmée M,Daube G

    更新日期:2016-10-01 00:00:00

  • Allele distribution and genetic diversity of VNTR loci in Salmonella enterica serotype Enteritidis isolates from different sources.

    abstract:BACKGROUND:Salmonella enterica serotype Enteritidis (S. Enteritidis) is a zoonotic pathogen, which can be found in many sources including animals and the environment. However, little is known about the molecular relatedness among S. Enteritidis isolates from different sources. We have applied multiple-locus variable nu...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-146

    authors: Cho S,Whittam TS,Boxrud DJ,Bartkus JM,Saeed AM

    更新日期:2008-09-15 00:00:00

  • Long-term impact of fecal transplantation in healthy volunteers.

    abstract:BACKGROUND:Fecal microbiota transplantation (FMT) has been recently approved by FDA for the treatment of refractory recurrent clostridial colitis (rCDI). Success of FTM in treatment of rCDI led to a number of studies investigating the effectiveness of its application in the other gastrointestinal diseases. However, in ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1689-y

    authors: Goloshchapov OV,Olekhnovich EI,Sidorenko SV,Moiseev IS,Kucher MA,Fedorov DE,Pavlenko AV,Manolov AI,Gostev VV,Veselovsky VA,Klimina KM,Kostryukova ES,Bakin EA,Shvetcov AN,Gumbatova ED,Klementeva RV,Shcherbakov AA,Gorchak

    更新日期:2019-12-30 00:00:00

  • Nuclear and Wolbachia-based multimarker approach for the rapid and accurate identification of tsetse species.

    abstract:BACKGROUND:Tsetse flies (Diptera: Glossinidae) are solely responsible for the transmission of African trypanosomes, causative agents of sleeping sickness in humans and nagana in livestock. Due to the lack of efficient vaccines and the emergence of drug resistance, vector control approaches such as the sterile insect te...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1295-4

    authors: Augustinos AA,Meki IK,Demirbas-Uzel G,Ouédraogo GMS,Saridaki A,Tsiamis G,Parker AG,Abd-Alla AMM,Bourtzis K

    更新日期:2018-11-23 00:00:00

  • Developmental succession of the microbiome of Culex mosquitoes.

    abstract:BACKGROUND:The native microflora associated with mosquitoes have important roles in mosquito development and vector competence. Sequencing of bacterial V3 region from 16S rRNA genes across the developmental stages of Culex mosquitoes (early and late larval instars, pupae and adults) was used to test the hypothesis that...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0475-8

    authors: Duguma D,Hall MW,Rugman-Jones P,Stouthamer R,Terenius O,Neufeld JD,Walton WE

    更新日期:2015-07-24 00:00:00

  • Identification of a bacteriocin and its cognate immunity factor expressed by Moraxella catarrhalis.

    abstract:BACKGROUND:Bacteriocins are antimicrobial proteins and peptides ribosomally synthesized by some bacteria which can effect both intraspecies and interspecies killing. RESULTS:Moraxella catarrhalis strain E22 containing plasmid pLQ510 was shown to inhibit the growth of M. catarrhalis strain O35E. Two genes (mcbA and mcb...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-207

    authors: Attia AS,Sedillo JL,Hoopman TC,Liu W,Liu L,Brautigam CA,Hansen EJ

    更新日期:2009-09-25 00:00:00

  • A blue native-PAGE analysis of membrane protein complexes in Clostridium thermocellum.

    abstract:BACKGROUND:Clostridium thermocellum is a Gram-positive thermophilic anaerobic bacterium with the unusual capacity to convert cellulosic biomass into ethanol and hydrogen. Identification and characterization of protein complexes in C. thermocellum are important toward understanding its metabolism and physiology. RESULT...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-11-22

    authors: Peng Y,Luo Y,Yu T,Xu X,Fan K,Zhao Y,Yang K

    更新日期:2011-01-26 00:00:00

  • Biofilm forming properties of quinolone resistant Escherichia coli from the broiler production chain and their dynamics in mixed biofilms.

    abstract:BACKGROUND:Quinolone resistant Escherichia coli (QREC) have been found in samples from Norwegian broiler chicken, despite quinolones not being administered to poultry in Norway. Biofilm production may be one factor contributing to the observed persistence in the broiler production chain. In the present study, 158 QREC ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01730-w

    authors: Nesse LL,Osland AM,Mo SS,Sekse C,Slettemeås JS,Bruvoll AEE,Urdahl AM,Vestby LK

    更新日期:2020-03-04 00:00:00

  • Geochip-based analysis of microbial communities in alpine meadow soils in the Qinghai-Tibetan plateau.

    abstract:BACKGROUND:GeoChip 3.0, a microbial functional gene array, containing ~28,000 oligonucleotide probes and targeting ~57,000 sequences from 292 functional gene families, provided a powerful tool for researching microbial community structure in natural environments. The alpine meadow is a dominant plant community in the Q...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-72

    authors: Zhang Y,Lu Z,Liu S,Yang Y,He Z,Ren Z,Zhou J,Li D

    更新日期:2013-03-29 00:00:00

  • The Salmonella Pathogenicity Island (SPI) 1 contributes more than SPI2 to the colonization of the chicken by Salmonella enterica serovar Typhimurium.

    abstract:BACKGROUND:Salmonella enterica serovar Typhimurium (Typhimurium) is an important pathogen that infects a broad range of hosts. In humans, Typhimurium causes a gastroenteritis characterized by vomiting, diarrhea, and abdominal pains. Typhimurium infection occurs mainly through the ingestion of contaminated food includin...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-3

    authors: Dieye Y,Ameiss K,Mellata M,Curtiss R 3rd

    更新日期:2009-01-06 00:00:00

  • Mycobacterium tuberculosis interactome analysis unravels potential pathways to drug resistance.

    abstract:BACKGROUND:Emergence of drug resistant varieties of tuberculosis is posing a major threat to global tuberculosis eradication programmes. Although several approaches have been explored to counter resistance, there has been limited success due to a lack of understanding of how resistance emerges in bacteria upon drug tre...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-234

    authors: Raman K,Chandra N

    更新日期:2008-12-23 00:00:00

  • Commensal Streptococcus agalactiae isolated from patients seen at University Hospital of Londrina, Paraná, Brazil: capsular types, genotyping, antimicrobial susceptibility and virulence determinants.

    abstract:BACKGROUND:Streptococcus agalactiae or Group B Streptococci (GBS) have the ability to access various host sites, which reflects its adaptability to different environments during the course of infection. This adaptation is due to the expression of virulence factors that are involved with survival, invasion and bacterial...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-297

    authors: Otaguiri ES,Morguette AE,Tavares ER,dos Santos PM,Morey AT,Cardoso JD,Perugini MR,Yamauchi LM,Yamada-Ogatta SF

    更新日期:2013-12-21 00:00:00

  • Survey of bacterial diversity in chronic wounds using pyrosequencing, DGGE, and full ribosome shotgun sequencing.

    abstract:BACKGROUND:Chronic wound pathogenic biofilms are host-pathogen environments that colonize and exist as a cohabitation of many bacterial species. These bacterial populations cooperate to promote their own survival and the chronic nature of the infection. Few studies have performed extensive surveys of the bacterial popu...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-43

    authors: Dowd SE,Sun Y,Secor PR,Rhoads DD,Wolcott BM,James GA,Wolcott RD

    更新日期:2008-03-06 00:00:00

  • Multi-locus phylogeny and taxonomy of an unresolved, heterogeneous species complex within the genus Golovinomyces (Ascomycota, Erysiphales), including G. ambrosiae, G. circumfusus and G. spadiceus.

    abstract:BACKGROUND:Previous phylogenetic analyses of species within the genus Golovinomyces (Ascomycota, Erysiphales), based on ITS and 28S rDNA sequence data, revealed a co-evolutionary relationship between powdery mildew species and hosts of certain tribes of the plant family Asteraceae. Golovinomyces growing on host plants ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01731-9

    authors: Qiu PL,Liu SY,Bradshaw M,Rooney-Latham S,Takamatsu S,Bulgakov TS,Tang SR,Feng J,Jin DN,Aroge T,Li Y,Wang LL,Braun U

    更新日期:2020-03-05 00:00:00

  • Identification and characterization of intervening sequences within 23S rRNA genes from more than 200 Campylobacter isolates from seven species including atypical campylobacters.

    abstract:BACKGROUND:Identification and characterization of intervening sequences (IVSs) within 23S rRNA genes from Campylobacter organisms including atypical campylobacters were carried out using two PCR primer pairs, designed to generate helix 25 and 45 regions. RESULTS:Only C. sputorum biovar sputorum LMG7975 and fecalis LMG...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-256

    authors: Tazumi A,Kakinuma Y,Misawa N,Moore JE,Millar BC,Matsuda M

    更新日期:2009-12-11 00:00:00

  • Persistent, triple-virus co-infections in mosquito cells.

    abstract:BACKGROUND:It is known that insects and crustaceans can carry simultaneous, active infections of two or more viruses without showing signs of disease, but it was not clear whether co-infecting viruses occupied the same cells or different cells in common target tissues. Our previous work showed that successive challenge...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-14

    authors: Kanthong N,Khemnu N,Pattanakitsakul SN,Malasit P,Flegel TW

    更新日期:2010-01-20 00:00:00

  • Proteome profiling of Pseudomonas aeruginosa PAO1 identifies novel responders to copper stress.

    abstract:BACKGROUND:The opportunistic pathogen, Pseudomonas aeruginosa is well known for its environmental and metabolic versatility, yet many of the functions of its gene-products remain to be fully elucidated. This study's objective was to illuminate the potential functions of under-described gene-products during the medicall...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1441-7

    authors: Wright BW,Kamath KS,Krisp C,Molloy MP

    更新日期:2019-04-01 00:00:00

  • Chemical modulators of the innate immune response alter gypsy moth larval susceptibility to Bacillus thuringiensis.

    abstract:BACKGROUND:The gut comprises an essential barrier that protects both invertebrate and vertebrate animals from invasion by microorganisms. Disruption of the balanced relationship between indigenous gut microbiota and their host can result in gut bacteria eliciting host responses similar to those caused by invasive patho...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-129

    authors: Broderick NA,Raffa KF,Handelsman J

    更新日期:2010-04-27 00:00:00

  • A recombinase system facilitates cloning of expression cassettes in the ciliate Tetrahymena thermophila.

    abstract:BACKGROUND:Tetrahymena thermophila is one of the best characterized unicellular eukaryotes and its genome is sequenced in its entirety. However, the AT-richness of the genome and an unusual codon usage cause problems in cloning and expression of the ciliate DNA. To overcome these technical hiatuses we developed a Cre-d...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-7-12

    authors: Weide T,Bockau U,Rave A,Herrmann L,Hartmann MW

    更新日期:2007-03-01 00:00:00

  • Predictive analysis of transmissible quinolone resistance indicates Stenotrophomonas maltophilia as a potential source of a novel family of Qnr determinants.

    abstract:BACKGROUND:Predicting antibiotic resistance before it emerges at clinical settings constitutes a novel approach for preventing and fighting resistance of bacterial pathogens. To analyse the possibility that novel plasmid-encoded quinolone resistance determinants (Qnr) can emerge and disseminate among bacterial pathogen...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-148

    authors: Sánchez MB,Hernández A,Rodríguez-Martínez JM,Martínez-Martínez L,Martínez JL

    更新日期:2008-09-16 00:00:00

  • Genetic analysis of the capsule polysaccharide (K antigen) and exopolysaccharide genes in pandemic Vibrio parahaemolyticus O3:K6.

    abstract:BACKGROUND:Pandemic Vibrio parahaemolyticus has undergone rapid changes in both K- and O-antigens, making detection of outbreaks more difficult. In order to understand these rapid changes, the genetic regions encoding these antigens must be examined. In Vibrio cholerae and Vibrio vulnificus, both O-antigen and capsular...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-274

    authors: Chen Y,Dai J,Morris JG Jr,Johnson JA

    更新日期:2010-11-02 00:00:00

  • Diversity of Mycobacterium tuberculosis and drug resistance in different provinces of Papua New Guinea.

    abstract:BACKGROUND:Papua New Guinea (PNG) is a high tuberculosis (TB) burden country of the WHO Western Pacific Region, but so far research on drug resistance (DR) and genotypes of Mycobacterium tuberculosis (M. tuberculosis) was only conducted in few provinces in the country. The aim of the present study was to obtain baselin...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-014-0307-2

    authors: Ley SD,Harino P,Vanuga K,Kamus R,Carter R,Coulter C,Pandey S,Feldmann J,Ballif M,Siba PM,Phuanukoonnon S,Gagneux S,Beck HP

    更新日期:2014-12-05 00:00:00

  • Comparative analysis of the growth and biological activity of a respiratory and atheroma isolate of Chlamydia pneumoniae reveals strain-dependent differences in inflammatory activity and innate immune evasion.

    abstract:BACKGROUND:Chlamydia pneumoniae is a common human pathogen that is associated with upper and lower respiratory tract infections. It has also been suggested that C. pneumoniae infection can trigger or promote a number of chronic inflammatory conditions, including asthma and atherosclerosis. Several strains of C. pneumon...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0569-3

    authors: He X,Liang Y,LaValley MP,Lai J,Ingalls RR

    更新日期:2015-10-23 00:00:00

  • Influence of wet distillers grains diets on beef cattle fecal bacterial community structure.

    abstract:BACKGROUND:The high demand for ethanol in the U.S. has generated large stocks of wet distillers grains (DG), a byproduct from the manufacture of ethanol from corn and sorghum grains. Little is known, however, about the potential influence of dietary DG on fecal microbial community structure. A better understanding of t...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-12-25

    authors: Rice WC,Galyean ML,Cox SB,Dowd SE,Cole NA

    更新日期:2012-02-24 00:00:00

  • Survival capabilities of Escherichia coli O26 isolated from cattle and clinical sources in Australia to disinfectants, acids and antimicrobials.

    abstract:BACKGROUND:After E. coli O157, E. coli O26 is the second most prevalent enterohaemorrhagic E. coli (EHEC) serotype identified in cases of foodborne illness in Australia and throughout the world. E. coli O26 associated foodborne outbreaks have drawn attention to the survival capabilities of this organism in a range of e...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-017-0963-0

    authors: Lajhar SA,Brownlie J,Barlow R

    更新日期:2017-03-01 00:00:00

  • The role of 3-ketosteroid 1(2)-dehydrogenase in the pathogenicity of Mycobacterium tuberculosis.

    abstract:BACKGROUND:A growing body of evidence suggests that Mycobacterium tuberculosis (Mtb) uses the host's cholesterol as a source of carbon and energy during infection. Strains defective in cholesterol transport or degradation exhibit attenuated growth in activated macrophages and diminished infectivity in animal models. Th...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-43

    authors: Brzezinska M,Szulc I,Brzostek A,Klink M,Kielbik M,Sulowska Z,Pawelczyk J,Dziadek J

    更新日期:2013-02-20 00:00:00

  • RpfC regulates the expression of the key regulator hrpX of the hrp/T3SS system in Xanthomonas campestris pv. campestris.

    abstract:BACKGROUND:The Gram-negative phytopathogenic bacterium Xanthomonas campestris pv. campestris recruits the hrp/T3SS system to inject pathogenicity effector proteins into host cells and uses the rpf/DSF cell-cell signaling system to regulate the expression of virulence factors such as extracellular enzymes and polysaccha...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1233-5

    authors: Jiang BL,Jiang GF,Liu W,Yang LC,Yang LY,Wang L,Hang XH,Tang JL

    更新日期:2018-09-03 00:00:00

  • Proteomic profiling of Rhizobium tropici PRF 81: identification of conserved and specific responses to heat stress.

    abstract:BACKGROUND:Rhizobium tropici strain PRF 81 (= SEMIA 4080) has been used in commercial inoculants for application to common-bean crops in Brazil since 1998, due to its high efficiency in fixing nitrogen, competitiveness against indigenous rhizobial populations and capacity to adapt to stressful tropical conditions, repr...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-12-84

    authors: Gomes DF,Batista JS,Schiavon AL,Andrade DS,Hungria M

    更新日期:2012-05-30 00:00:00