Comparative analysis of the growth and biological activity of a respiratory and atheroma isolate of Chlamydia pneumoniae reveals strain-dependent differences in inflammatory activity and innate immune evasion.


BACKGROUND:Chlamydia pneumoniae is a common human pathogen that is associated with upper and lower respiratory tract infections. It has also been suggested that C. pneumoniae infection can trigger or promote a number of chronic inflammatory conditions, including asthma and atherosclerosis. Several strains of C. pneumoniae have been isolated from humans and animals, and sequence data demonstrates marked genetic conservation, leaving unanswered the question as to why chronic inflammatory conditions may occur following some respiratory-acquired infections. METHODS:C. pneumoniae strains AR39 and AO3 were used in vitro to infect murine bone marrow derived macrophages and L929 fibroblasts, or in vivo to infect C57BL/6 mice via the intranasal route. RESULTS:We undertook a comparative study of a respiratory isolate, AR39, and an atheroma isolate, AO3, to determine if bacterial growth and host responses to infection varied between these two strains. We observed differential growth depending on the host cell type and the growth temperature; however both strains were capable of forming plaques in vitro. The host response to the respiratory isolate was found to be more inflammatory both in vitro, in terms of inflammatory cytokine induction, and in vivo, as measured by clinical response and lung inflammatory markers using a mouse model of respiratory infection. CONCLUSIONS:Our data demonstrates that a subset of C. pneumoniae strains is capable of evading host innate immune defenses during the acute respiratory infection. Further studies on the genetic basis for these differences on both the host and pathogen side could enhance our understanding how C. pneumoniae contributes to the development chronic inflammation at local and distant sites.


BMC Microbiol


BMC microbiology


He X,Liang Y,LaValley MP,Lai J,Ingalls RR




Has Abstract


2015-10-23 00:00:00










  • Biofilm formation as a novel phenotypic feature of adherent-invasive Escherichia coli (AIEC).

    abstract:BACKGROUND:Crohn's disease (CD) is a high morbidity chronic inflammatory disorder of unknown aetiology. Adherent-invasive Escherichia coli (AIEC) has been recently implicated in the origin and perpetuation of CD. Because bacterial biofilms in the gut mucosa are suspected to play a role in CD and biofilm formation is a ...

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    pub_type: 杂志文章


    authors: Martinez-Medina M,Naves P,Blanco J,Aldeguer X,Blanco JE,Blanco M,Ponte C,Soriano F,Darfeuille-Michaud A,Garcia-Gil LJ

    更新日期:2009-09-21 00:00:00

  • Colony shape as a genetic trait in the pattern-forming Bacillus mycoides.

    abstract:BACKGROUND:Bacillus mycoides Flügge, a Gram-positive, non-motile soil bacterium assigned to Bacillus cereus group, grows on agar as chains of cells linked end to end, forming radial filaments curving clock- or counter-clockwise (SIN or DX morphotypes). The molecular mechanism causing asymmetric curving is not known: ou...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Di Franco C,Beccari E,Santini T,Pisaneschi G,Tecce G

    更新日期:2002-11-13 00:00:00

  • Antimicrobial activity of copper surfaces against suspensions of Salmonella enterica and Campylobacter jejuni.

    abstract:BACKGROUND:Salmonella enterica and Campylobacter jejuni are amongst the more prevalent bacterial pathogens that cause foodborne diseases. These microorganisms are common contaminants of poultry and poultry products. This study was aimed to evaluate the antibacterial activity of metallic copper surfaces on these importa...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Faúndez G,Troncoso M,Navarrete P,Figueroa G

    更新日期:2004-04-30 00:00:00

  • Genomic analyses of two novel biofilm-degrading methicillin-resistant Staphylococcus aureus phages.

    abstract:BACKGROUND:Methicillin-resistant Staphylococcus aureus (MRSA) biofilm producers represent an important etiological agent of many chronic human infections. Antibiotics and host immune responses are largely ineffective against bacteria within biofilms. Alternative actions and novel antimicrobials should be considered. In...

    journal_title:BMC microbiology

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    authors: Dakheel KH,Rahim RA,Neela VK,Al-Obaidi JR,Hun TG,Isa MNM,Yusoff K

    更新日期:2019-05-28 00:00:00

  • Statistical characterization of the GxxxG glycine repeats in the flagellar biosynthesis protein FliH and its Type III secretion homologue YscL.

    abstract:BACKGROUND:FliH is a protein involved in the export of components of the bacterial flagellum and we herein describe the presence of glycine-rich repeats in FliH of the form AxxxG(xxxG)mxxxA, where the value of m varies considerably in FliH proteins from different bacteria. While GxxxG and AxxxA patterns have previously...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Trost B,Moore SA

    更新日期:2009-04-16 00:00:00

  • Pyviko: an automated Python tool to design gene knockouts in complex viruses with overlapping genes.

    abstract:BACKGROUND:Gene knockouts are a common tool used to study gene function in various organisms. However, designing gene knockouts is complicated in viruses, which frequently contain sequences that code for multiple overlapping genes. Designing mutants that can be traced by the creation of new or elimination of existing r...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Taylor LJ,Strebel K

    更新日期:2017-01-07 00:00:00

  • Influence of plant diversity and elevated atmospheric carbon dioxide levels on belowground bacterial diversity.

    abstract:BACKGROUND:Changes in aboveground plant species diversity as well as variations of environmental conditions such as exposure of ecosystems to elevated concentrations of atmospheric carbon dioxide may lead to changes in metabolic activity, composition and diversity of belowground microbial communities, both bacterial an...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Grüter D,Schmid B,Brandl H

    更新日期:2006-07-27 00:00:00

  • Role of type 1 and type 3 fimbriae in Klebsiella pneumoniae biofilm formation.

    abstract:BACKGROUND:Klebsiella pneumoniae is an important gram-negative opportunistic pathogen causing primarily urinary tract infections, respiratory infections, and bacteraemia. The ability of bacteria to form biofilms on medical devices, e.g. catheters, has a major role in development of many nosocomial infections. Most clin...

    journal_title:BMC microbiology

    pub_type: 杂志文章


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    更新日期:2010-06-23 00:00:00

  • Effect of spaceflight on Pseudomonas aeruginosa final cell density is modulated by nutrient and oxygen availability.

    abstract:BACKGROUND:Abundant populations of bacteria have been observed on Mir and the International Space Station. While some experiments have shown that bacteria cultured during spaceflight exhibit a range of potentially troublesome characteristics, including increases in growth, antibiotic resistance and virulence, other stu...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Kim W,Tengra FK,Shong J,Marchand N,Chan HK,Young Z,Pangule RC,Parra M,Dordick JS,Plawsky JL,Collins CH

    更新日期:2013-11-06 00:00:00

  • Safety evaluation of the antimicrobial peptide bovicin HC5 orally administered to a murine model.

    abstract:BACKGROUND:Bovicin HC5 is an antimicrobial peptide that shows a broad spectrum of activity and potential for biotechnological and therapeutic applications. To gain insight about the safety of bovicin HC5 application, the histological and immunostimulatory effects of orally administrated bovicin HC5 to BALB/c mice were ...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Paiva AD,Fernandes KM,Dias RS,Rocha Ados S,Oliveira LL,Neves CA,Paula SO,Mantovani HC

    更新日期:2013-03-27 00:00:00

  • Study on the community structure and function of symbiotic bacteria from different growth and developmental stages of Hypsizygus marmoreus.

    abstract:BACKGROUND:The symbiotic bacteria associated with edible fungi are valuable microbial resources worthy of in-depth exploration. It is important to analyze the community structure and succession of symbiotic bacteria in mushrooms. This can assist in the isolation of growth-promoting strains that have an essential relati...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Sun S,Li F,Xu X,Liu Y,Kong X,Chen J,Liu T,Chen L

    更新日期:2020-10-14 00:00:00

  • Phylogenetic group- and species-specific oligonucleotide probes for single-cell detection of lactic acid bacteria in oral biofilms.

    abstract:BACKGROUND:The purpose of this study was to design and evaluate fluorescent in situ hybridization (FISH) probes for the single-cell detection and enumeration of lactic acid bacteria, in particular organisms belonging to the major phylogenetic groups and species of oral lactobacilli and to Abiotrophia/Granulicatella. R...

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    pub_type: 杂志文章


    authors: Quevedo B,Giertsen E,Zijnge V,Lüthi-Schaller H,Guggenheim B,Thurnheer T,Gmür R

    更新日期:2011-01-19 00:00:00

  • The SspA subtilisin-like protease of Streptococcus suis triggers a pro-inflammatory response in macrophages through a non-proteolytic mechanism.

    abstract:BACKGROUND:Streptococcus suis is a major swine pathogen worldwide that causes meningitis, septicemia, arthritis, and endocarditis. Using animal models, a surface-associated subtilisin-like protease (SspA) has recently been shown to be an important virulence factor for S. suis. In this study, we hypothesized that the S....

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Bonifait L,Grenier D

    更新日期:2011-03-01 00:00:00

  • Bactericidal activity of PA-824 against Mycobacterium tuberculosis under anaerobic conditions and computational analysis of its novel analogues against mutant Ddn receptor.

    abstract:BACKGROUND:The resurgence of multi-drug resistant tuberculosis (MDR-TB) and HIV associated tuberculosis (TB) are of serious global concern. To contain this situation, new anti-tuberculosis drugs and reduced treatment regimens are imperative. Recently, a nitroimidazole, PA-824, has been shown to be active against both r...

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    pub_type: 杂志文章


    authors: Somasundaram S,Anand RS,Venkatesan P,Paramasivan CN

    更新日期:2013-10-01 00:00:00

  • The ColRS signal transduction system responds to the excess of external zinc, iron, manganese, and cadmium.

    abstract:BACKGROUND:The ColRS two-component system has been shown to contribute to the membrane functionality and stress tolerance of Pseudomonas putida as well as to the virulence of Pseudomonas aeruginosa and plant pathogenic Xanthomonas species. However, the conditions activating the ColRS pathway and the signal(s) sensed by...

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    pub_type: 杂志文章


    authors: Ainsaar K,Mumm K,Ilves H,Hõrak R

    更新日期:2014-06-20 00:00:00

  • In silico evolutionary analysis of Helicobacter pylori outer membrane phospholipase A (OMPLA).

    abstract:BACKGROUND:In the past decade, researchers have proposed that the pldA gene for outer membrane phospholipase A (OMPLA) is important for bacterial colonization of the human gastric ventricle. Several conserved Helicobacter pylori genes have distinct genotypes in different parts of the world, biogeographic patterns that ...

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    pub_type: 杂志文章


    authors: Vollan HS,Tannaes T,Yamaoka Y,Bukholm G

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  • Identification of a Golgi-localized UDP-N-acetylglucosamine transporter in Trypanosoma cruzi.

    abstract:BACKGROUND:Nucleotide sugar transporters (NSTs) play an essential role in translocating nucleotide sugars into the lumen of the endoplasmic reticulum and Golgi apparatus to be used as substrates in glycosylation reactions. This intracellular transport is an essential step in the biosynthesis of glycoconjugates. RESULT...

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    pub_type: 杂志文章


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    更新日期:2015-11-21 00:00:00

  • Planctomycetes dominate biofilms on surfaces of the kelp Laminaria hyperborea.

    abstract:BACKGROUND:Bacteria belonging to Planctomycetes display several unique morphological and genetic features and are found in a wide variety of habitats on earth. Their ecological roles in these habitats are still poorly understood. Planctomycetes have previously been detected throughout the year on surfaces of the kelp L...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Bengtsson MM,Øvreås L

    更新日期:2010-10-15 00:00:00

  • Transcriptional analysis of Mycobacterium fortuitum cultures upon hydrogen peroxide treatment using the novel standard rrnA-P1.

    abstract:BACKGROUND:The ability of an intracellular pathogen to establish infection depends on the capacity of the organism to survive and replicate inside the host. Mycobacterium fortuitum is a bacteria that contains genes involved in the detoxification of the oxygen reactive species such as those produced by the host during t...

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    pub_type: 杂志文章


    authors: Núñez MC,Menéndez MC,Rebollo MJ,García MJ

    更新日期:2008-06-19 00:00:00

  • Roles of RpoN in the resistance of Campylobacter jejuni under various stress conditions.

    abstract:BACKGROUND:Campylobacter jejuni is a leading foodborne pathogen worldwide. Despite the fastidious nature of C. jejuni growth, increasing numbers of human campylobacteriosis suggest that C. jejuni may possess unique mechanisms to survive under various stress conditions. C. jejuni possesses only three sigma factors (FliA...

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    pub_type: 杂志文章


    authors: Hwang S,Jeon B,Yun J,Ryu S

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  • VirB10 vaccination for protection against Anaplasma phagocytophilum.

    abstract:BACKGROUND:Human granulocytic anaplasmosis (HGA) is a tick-borne disease caused by the etiologic agent Anaplasma phagocytophilum. HGA was designated a nationally notifiable disease in the United States in 1998. Currently there are no vaccines available against HGA. Conserved membrane proteins that are subdominant in An...

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    authors: Crosby FL,Lundgren AM,Hoffman C,Pascual DW,Barbet AF

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  • Assessment of CcpA-mediated catabolite control of gene expression in Bacillus cereus ATCC 14579.

    abstract:BACKGROUND:The catabolite control protein CcpA is a transcriptional regulator conserved in many Gram-positives, controlling the efficiency of glucose metabolism. Here we studied the role of Bacillus cereus ATCC 14579 CcpA in regulation of metabolic pathways and expression of enterotoxin genes by comparative transcripto...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: van der Voort M,Kuipers OP,Buist G,de Vos WM,Abee T

    更新日期:2008-04-16 00:00:00

  • Assessing diversity of the female urine microbiota by high throughput sequencing of 16S rDNA amplicons.

    abstract:BACKGROUND:Urine within the urinary tract is commonly regarded as "sterile" in cultivation terms. Here, we present a comprehensive in-depth study of bacterial 16S rDNA sequences associated with urine from healthy females by means of culture-independent high-throughput sequencing techniques. RESULTS:Sequencing of the V...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Siddiqui H,Nederbragt AJ,Lagesen K,Jeansson SL,Jakobsen KS

    更新日期:2011-11-02 00:00:00

  • Determination of antibacterial activity and minimum inhibitory concentration of larval extract of fly via resazurin-based turbidometric assay.

    abstract:BACKGROUND:Antimicrobial resistance is currently a major global issue. As the rate of emergence of antimicrobial resistance has superseded the rate of discovery and introduction of new effective drugs, the medical arsenal now is experiencing shortage of effective drugs to combat diseases, particularly against diseases ...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Teh CH,Nazni WA,Nurulhusna AH,Norazah A,Lee HL

    更新日期:2017-02-16 00:00:00

  • Bactericidal activity of M protein conserved region antibodies against group A streptococcal isolates from the Northern Thai population.

    abstract:BACKGROUND:Most group A streptococcal (GAS) vaccine strategies have focused on the surface M protein, a major virulence factor of GAS. The amino-terminus of the M protein elicits antibodies, that are both opsonic and protective, but which are type specific. J14, a chimeric peptide that contains 14 amino acids from the ...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Yoonim N,Olive C,Pruksachatkunakorn C,Pruksakorn S

    更新日期:2006-08-09 00:00:00

  • Community terminal restriction fragment length polymorphisms reveal insights into the diversity and dynamics of leaf endophytic bacteria.

    abstract:BACKGROUND:Plant endophytic bacteria play an important role benefiting plant growth or being pathogenic to plants or organisms that consume those plants. Multiple species of bacteria have been found co-inhabiting plants, both cultivated and wild, with viruses and fungi. For these reasons, a general understanding of pla...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Ding T,Palmer MW,Melcher U

    更新日期:2013-01-03 00:00:00

  • Diet, physical activity and screen time but not body mass index are associated with the gut microbiome of a diverse cohort of college students living in university housing: a cross-sectional study.

    abstract:BACKGROUND:Modifiable lifestyle factors (e.g. dietary intake and physical activity) are important contributors to weight gain during college. The purpose of this study was to evaluate whether associations exist between body mass index, physical activity, screen time, dietary consumption (fat, protein, carbohydrates, an...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Whisner CM,Maldonado J,Dente B,Krajmalnik-Brown R,Bruening M

    更新日期:2018-12-12 00:00:00

  • Galectins from Onchocerca ochengi and O. volvulus and their immune recognition by Wistar rats, Gudali zebu cattle and human hosts.

    abstract:BACKGROUND:During the last two decades research on animal filarial parasites, especially Onchocerca ochengi, infecting cattle in savanna areas of Africa revealed that O. ochengi as an animal model has biological features that are similar to those of O. volvulus, the aetiological agent of human onchocerciasis. There is,...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Ngwasiri NN,Brattig NW,Ndjonka D,Liebau E,Paguem A,Leusder D,Kingsley MT,Eisenbarth A,Renz A,Daniel AM

    更新日期:2021-01-06 00:00:00

  • Protein expression dynamics during Escherichia coli glucose-lactose diauxie.

    abstract:BACKGROUND:Escherichia coli is a well-studied anaerobic bacteria which is able to regulate metabolic pathways depending on the type of sugar presented in the medium. We have studied the glucose-lactose shift in E. coli at the protein level using a recently developed mass spectrometry platform. METHOD:Cells were grown ...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Mostovenko E,Deelder AM,Palmblad M

    更新日期:2011-06-01 00:00:00

  • Role of Mycobacterium tuberculosis pknD in the pathogenesis of central nervous system tuberculosis.

    abstract:BACKGROUND:Central nervous system disease is the most serious form of tuberculosis, and is associated with high mortality and severe neurological sequelae. Though recent clinical reports suggest an association of distinct Mycobacterium tuberculosis strains with central nervous system disease, the microbial virulence fa...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Be NA,Bishai WR,Jain SK

    更新日期:2012-01-13 00:00:00