Abstract:
BACKGROUND:The complete genome sequences of 44 Bacillus cereus group isolates collected from diverse sources in Japan were analyzed to determine their genetic backgrounds and diversity levels in Japan. Multilocus sequence typing (MLST) and core-genome single-nucleotide polymorphism (SNP) typing data from whole-genome sequences were analyzed to determine genetic diversity levels. Virulence-associated gene profiles were also used to evaluate the genetic backgrounds and relationships among the isolates. RESULTS:The 44 B. cereus group isolates, including soil- and animal-derived isolates and isolates recovered from hospitalized patients and food poisoning cases, were genotyped by MLST and core-genome SNP typing. Genetic variation among the isolates was identified by the MLST and core-genome SNP phylogeny comparison against reference strains from countries outside of Japan. Exploratory principal component analysis and nonmetric multidimensional scaling (NMDS) analyses were used to assess the genetic similarities among the isolates using gene presence and absence information and isolate origins as the metadata. A significant correlation was seen between the principal components and the presence of genes encoding hemolysin BL and emetic genetic determinants in B. cereus, and the capsule proteins in B. anthracis. NMDS showed that the cluster of soil isolates overlapped with the cluster comprising animal-derived and clinical isolates. CONCLUSIONS:Molecular and epidemiological analyses of B. cereus group isolates in Japan suggest that the soil- and animal-derived bacteria from our study are not a significant risk to human health. However, because several of the clinical isolates share close genetic relationships with the environmental isolates, both molecular and epidemiological surveillance studies could be used effectively to estimate virulence in these important pathogens.
journal_name
BMC Microbioljournal_title
BMC microbiologyauthors
Okutani A,Inoue S,Noguchi A,Kaku Y,Morikawa Sdoi
10.1186/s12866-019-1678-1subject
Has Abstractpub_date
2019-12-16 00:00:00pages
296issue
1issn
1471-2180pii
10.1186/s12866-019-1678-1journal_volume
19pub_type
杂志文章相关文献
BMC MICROBIOLOGY文献大全abstract:BACKGROUND:Bordetella dermonecrotic toxin (DNT) causes the turbinate atrophy in swine atrophic rhinitis, caused by a Bordetella bronchiseptica infection of pigs, by inhibiting osteoblastic differentiation. The toxin is not actively secreted from the bacteria, and is presumed to be present in only small amounts in infec...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-247
更新日期:2010-09-25 00:00:00
abstract:BACKGROUND:Multi-locus sequence typing (MLST) is a portable, broadly applicable method for classifying bacterial isolates at an intra-species level. This methodology provides clinical and scientific investigators with a standardized means of monitoring evolution within bacterial populations. MLST uses the DNA sequences...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-14-143
更新日期:2014-06-03 00:00:00
abstract:BACKGROUND:Streptococcus suis is a major swine pathogen worldwide that causes meningitis, septicemia, arthritis, and endocarditis. Using animal models, a surface-associated subtilisin-like protease (SspA) has recently been shown to be an important virulence factor for S. suis. In this study, we hypothesized that the S....
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-11-47
更新日期:2011-03-01 00:00:00
abstract:BACKGROUND:Macrolides and lincosamides are two leading types of antibiotics commonly used in therapies. The study examines the differences in resistance to these antibiotics and their molecular bases in S. epidermidis as well as in rarely isolated species of coagulase-negative staphylococci such as S. hominis, S. haemo...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1496-5
更新日期:2019-06-10 00:00:00
abstract:BACKGROUND:The increase in resistance to antibiotics among disease-causing bacteria necessitates the development of alternative antimicrobial approaches such as the use of light-activated antimicrobial agents (LAAAs). Light of an appropriate wavelength activates the LAAA to produce cytotoxic species which can then caus...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-111
更新日期:2008-07-01 00:00:00
abstract:BACKGROUND:Enterobacterial common antigen (ECA) is a family-specific surface antigen shared by all members of the Enterobacteriaceae family. Previous studies showed that the loss of ECA results in Salmonella attenuation, indicating its usefulness as a vaccine candidate for Salmonella infection, but no studies have show...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01837-0
更新日期:2020-06-08 00:00:00
abstract:BACKGROUND:FliH is a protein involved in the export of components of the bacterial flagellum and we herein describe the presence of glycine-rich repeats in FliH of the form AxxxG(xxxG)mxxxA, where the value of m varies considerably in FliH proteins from different bacteria. While GxxxG and AxxxA patterns have previously...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-72
更新日期:2009-04-16 00:00:00
abstract:BACKGROUND:Aspergillus fumigatus is a ubiquitous saprotrophic fungus and an opportunistic pathogen of humans and animals. Humans and animals can inhale hundreds of A. fumigatus spores daily. Normally this is harmless for humans, but in case of immunodeficiency, invasive pulmonary aspergillosis (IPA) can develop with a ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1244-2
更新日期:2018-09-17 00:00:00
abstract:BACKGROUND:There is a rapid emergence of multiple resistant gram-negative bacteria due to overuse of antibiotics in the treatment of infections. Biofilms consist of polymicrobial communities that survive the host's defense system. The key bacteria in biofilms are slow growing and support an attachment and rapid growth ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0821-5
更新日期:2016-09-09 00:00:00
abstract:BACKGROUND:Vibrio anguillarum is an extracellular bacterial pathogen that is a causative agent of vibriosis in finfish and crustaceans with mortality rates ranging from 30% to 100%. Mutations in central metabolism (glycolysis and the TCA cycle) of intracellular pathogens often result in attenuated virulence due to depl...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-1124-1
更新日期:2017-11-14 00:00:00
abstract:BACKGROUND:The development of vaccines and evaluation of novel treatment strategies for invasive group A streptococcal (iGAS) disease requires suitable models of human infection that can be monitored longitudinally and are preferably non-invasive. Bio-photonic imaging provides an opportunity to reduce use of animals in...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1200-1
更新日期:2018-06-19 00:00:00
abstract:BACKGROUND:Pneumococcus is exposed to a variety of temperature and oxygen levels in the upper respiratory tract and as it invades the lung, tissues, and blood. We sought to determine the effect of environmental variability on growth in vitro and to assess variability between strains. We evaluated the effect of temperat...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1671-8
更新日期:2019-12-26 00:00:00
abstract:BACKGROUND:During the last two decades research on animal filarial parasites, especially Onchocerca ochengi, infecting cattle in savanna areas of Africa revealed that O. ochengi as an animal model has biological features that are similar to those of O. volvulus, the aetiological agent of human onchocerciasis. There is,...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-02064-3
更新日期:2021-01-06 00:00:00
abstract:BACKGROUND:Xanthophyllomyces dendrorhous is a basidiomycetous yeast that synthesizes astaxanthin, a carotenoid with great biotechnological impact. The ergosterol and carotenoid synthetic pathways derive from the mevalonate pathway and involve cytochrome P450 enzymes. Among these enzymes, the CYP51 family, which is invo...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0428-2
更新日期:2015-04-25 00:00:00
abstract:BACKGROUND:Classical bacteriological characteristics of Salmonella enterica indicate that the members of this species are unable to utilize lactose as a carbon source. However, lactose-fermenting (Lac+) strains of several Salmonella serovars have been isolated from different foodborne outbreaks as well as different geo...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0511-8
更新日期:2015-08-25 00:00:00
abstract:BACKGROUND:The sequenced O45:K1:H7 Escherichia coli meningitis strain S88 harbors a large virulence plasmid. To identify possible genetic determinants of pS88 virulence, we examined the transcriptomes of 88 plasmidic ORFs corresponding to known and putative virulence genes, and 35 ORFs of unknown function. RESULTS:Qua...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-115
更新日期:2012-06-21 00:00:00
abstract:BACKGROUND:Probiotics are increasingly applied to prevent and treat a range of infectious, immune related and gastrointestinal diseases. Despite this, the mechanisms behind the putative effects of probiotics are poorly understood. One of the suggested modes of probiotic action is modulation of the endogenous gut microb...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-1090-7
更新日期:2017-08-17 00:00:00
abstract:BACKGROUND:Legionella testing conducted at environmental laboratories plays an essential role in assessing the risk of disease transmission associated with water systems. However, drawbacks of culture-based methodology used for Legionella enumeration can have great impact on the results and interpretation which togethe...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0423-7
更新日期:2015-04-29 00:00:00
abstract:BACKGROUND:The role of Aspergillus fumigatus mycotoxins in the colonization of the respiratory tract by conidia has not been studied extensively, even though patients at risk from invasive aspergillosis frequently exhibit respiratory epithelium damage. In a previous study, we found that filtrates of A. fumigatus cultur...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-7-5
更新日期:2007-01-23 00:00:00
abstract:BACKGROUND:The genus Lactobacillus belongs to a large heterogeneous group of low G + C Gram-positive anaerobic bacteria, which are frequently used as probiotics. The health-beneficial effects, in particular the immunomodulation effect, of probiotics depend on the strain and dose used. Strain variations may be related t...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0631-9
更新日期:2016-02-09 00:00:00
abstract:BACKGROUND:It is known that insects and crustaceans can carry simultaneous, active infections of two or more viruses without showing signs of disease, but it was not clear whether co-infecting viruses occupied the same cells or different cells in common target tissues. Our previous work showed that successive challenge...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-14
更新日期:2010-01-20 00:00:00
abstract:BACKGROUND:Accumulation of gyrase cleavage complex in Escherichia coli from the action of quinolone antibiotics induces an oxidative damage cell death pathway. The oxidative cell death pathway has also been shown to be involved in the lethality following accumulation of cleavage complex formed by bacterial topoisomeras...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-11-261
更新日期:2011-12-12 00:00:00
abstract:BACKGROUND:Lactobacillus species are used as probiotics and play an important role in fermented food production. However, use of 16S rRNA gene sequences as standard markers for the differentiation of Lactobacillus species offers a very limited scope, as several species of Lactobacillus share similar 16S rRNA gene seque...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01781-z
更新日期:2020-04-15 00:00:00
abstract:BACKGROUND:Sinorhizobium meliloti is an agriculturally important model symbiont. There is an ongoing need to update and improve its genome annotation. In this study, we used a high-throughput pyrosequencing approach to sequence the transcriptome of S. meliloti, and search for new bacterial genes missed in the previous ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-72
更新日期:2008-05-02 00:00:00
abstract:BACKGROUND:The natural product Emodin demonstrates a wide range of pharmacological properties including anticancer, anti-inflammatory, antiproliferation, vasorelaxant and anti-H. pylori activities. Although its H. pylori inhibition was discovered, no acting target information against Emodin has been revealed to date. ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-91
更新日期:2009-05-12 00:00:00
abstract:BACKGROUND:Pyruvate oxidase (Pox) is an important enzyme in bacterial metabolism for increasing ATP production and providing a fitness advantage via hydrogen peroxide production. However, few Pox enzymes have been characterized from bacterial species. The tetrameric non-hydrogen-peroxide producing Pox from E. coli is a...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01788-6
更新日期:2020-05-24 00:00:00
abstract:BACKGROUND:Plant cell wall degrading enzymes (PCWDEs) are a subset of carbohydrate-active enzymes (CAZy) produced by plant pathogens to degrade plant cell walls. To counteract PCWDEs, plants release PCWDEs inhibitor proteins (PIPs) to reduce their impact. Several transgenic plants expressing exogenous PIPs that interac...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0761-0
更新日期:2016-07-12 00:00:00
abstract:BACKGROUND:Enteroaggregative (EAEC) and Shiga-toxin producing Escherichia coli (STEC) are a major cause of diarrhea worldwide. E. coli carrying both virulence factors characteristic for EAEC and STEC and producing extended-spectrum beta-lactamase caused severe and protracted disease during an outbreak of E. coli O104:H...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0827-z
更新日期:2016-09-09 00:00:00
abstract:BACKGROUND:Microencapsulated organic acids and botanicals have the potential to develop into important tools for the poultry industry. A blend of organic acids and botanicals (AviPlus®P) has previously shown to reduce Salmonella and Campylobacter in chickens; however, changes to the microbiota of the jejunum and ileum ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-02001-4
更新日期:2020-11-02 00:00:00
abstract:BACKGROUND:Extended-spectrum cephalosporin resistance (ESC-R) in Escherichia coli and Klebsiella pneumoniae is a healthcare threat; high gastrointestinal carriage rates are reported from South-east Asia. Colonisation prevalence data in Cambodia are lacking. The aim of this study was to determine gastrointestinal coloni...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1431-9
更新日期:2019-03-12 00:00:00