Invasion of Ureaplasma diversum in Hep-2 cells.

Abstract:

BACKGROUND:Understanding mollicutes is challenging due to their variety and relationship with host cells. Invasion has explained issues related to their opportunistic role. Few studies have been done on the Ureaplasma diversum mollicute, which is detected in healthy or diseased bovine. The invasion in Hep-2 cells of four clinical isolates and two reference strains of their ureaplasma was studied by Confocal Laser Scanning Microscopy and gentamicin invasion assay. RESULTS:The isolates and strains used were detected inside the cells after infection of one minute without difference in the arrangement for adhesion and invasion. The adhesion was scattered throughout the cells, and after three hours, the invasion of the ureaplasmas surrounded the nuclear region but were not observed inside the nuclei. The gentamicin invasion assay detected that 1% of the ATCC strains were inside the infected Hep-2 cells in contrast to 10% to the clinical isolates. A high level of phospholipase C activity was also detected in all studied ureaplasma. CONCLUSIONS:The results presented herein will help better understand U. diversum infections, aswell as cellular attachment and virulence.

journal_name

BMC Microbiol

journal_title

BMC microbiology

authors

Marques LM,Ueno PM,Buzinhani M,Cortez BA,Neto RL,Yamaguti M,Oliveira RC,Guimarães AM,Monezi TA,Braga AC Jr,Machado-Santelli GM,Timenetsky J

doi

10.1186/1471-2180-10-83

subject

Has Abstract

pub_date

2010-03-17 00:00:00

pages

83

issn

1471-2180

pii

1471-2180-10-83

journal_volume

10

pub_type

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