Abstract：
BACKGROUND:Salmonella enterica is a causative agent of foodborne gastroenteritis and the systemic disease known as typhoid fever. This bacterium uses two type three secretion systems (T3SSs) to translocate protein effectors into host cells to manipulate cellular function. Salmonella pathogenicity island (SPI)-2 encodes a T3SS required for intracellular survival of the pathogen. Genes in SPI-2 include apparatus components, secreted effectors and chaperones that bind to secreted cargo to coordinate their release from the bacterial cell. Although the effector repertoire secreted by the SPI-2 T3SS is large, only three virulence-associated chaperones have been characterized. RESULTS:Here we report that SscA is the chaperone for the SseC translocon component. We show that SscA and SseC interact in bacterial cells and that deletion of sscA results in a loss of SseC secretion, which compromises intracellular replication and leads to a loss of competitive fitness in mice. CONCLUSIONS:This work completes the characterization of the chaperone complement within SPI-2 and identifies SscA as the chaperone for the SseC translocon.
journal_name
BMC Microbioljournal_title
BMC microbiologyauthors
Cooper CA,Mulder DT,Allison SE,Pilar AV,Coombes BKdoi
10.1186/1471-2180-13-221subject
Has Abstractpub_date
2013-10-04 00:00:00pages
221issn
1471-2180pii
1471-2180-13-221journal_volume
13pub_type
杂志文章相关文献
BMC MICROBIOLOGY文献大全abstract：BACKGROUND:Cystic fibrosis (CF) lung disease has a unique profile of pathogens predominated by Pseudomonas aeruginosa (PsA) and Staphylococcus aureus (SA). These microorganisms must overcome host immune defense to colonize the CF lungs. Polymorphonuclear neutrophils are a major component of the host defense against bac...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-11-112
更新日期：2011-05-20 00:00:00
abstract：BACKGROUND:Emergence of drug resistant varieties of tuberculosis is posing a major threat to global tuberculosis eradication programmes. Although several approaches have been explored to counter resistance, there has been limited success due to a lack of understanding of how resistance emerges in bacteria upon drug tre...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-8-234
更新日期：2008-12-23 00:00:00
abstract：BACKGROUND:The aim of this study was to identify mutations of rpoB, katG, inhA and ahp-genes associated Mycobacterium tuberculosis resistance to rifampicin (RIF) and isoniazid (INH) in Kyrgyz Republic. We studied 633 smear samples from the primary pulmonary tuberculosis (TB) patients. We verified Mycobacterium tubercul...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-018-1168-x
更新日期：2018-03-22 00:00:00
abstract：BACKGROUND:The currently used genotyping system for the avian zoonotic pathogen Chlamydophila (C.) psittaci has evolved from serology and is based on ompA sequence variations. It includes seven avian and two non-avian genotypes. Restriction enzyme cleavage of the amplified ompA gene and, less frequently, ompA sequencin...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-8-63
更新日期：2008-04-17 00:00:00
abstract：BACKGROUND:Bacteria employ multiple mechanisms to control gene expression and react to their constantly changing environment. Bacterial growth in rich laboratory medium is a dynamic process in which bacteria utilize nutrients from simple to complex and change physical properties of the medium, as pH, during the process...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-9-32
更新日期：2009-02-10 00:00:00
abstract：BACKGROUND:adhC from Haemophilus influenzae encodes a glutathione-dependent alcohol dehydrogenase that has previously been shown to be required for protection against killing by S-nitrosoglutathione (GSNO). This group of enzymes is known in other systems to be able to utilize substrates that form adducts with glutathio...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-12-159
更新日期：2012-07-31 00:00:00
abstract：ERRATUM:Upon publication of the original article (1) it was highlighted by the authors that a grant awarded to support the research work of the study was missed in the acknowledgements. It should also be acknowledged that the grant titled "Genotyping and Molecular Epidemiological Characteristic of Bacillus anthracis in...
journal_title：BMC microbiology
pub_type： 杂志文章,已发布勘误
doi：10.1186/s12866-018-1172-1
更新日期：2018-04-12 00:00:00
abstract：BACKGROUND:The genus Phytophthora includes a group of agriculturally important pathogens and they are commonly regarded as water molds. They produce motile zoospores that can move via water currents and on their own locomotion in aquatic environments. However, zoosporic response to dissolved oxygen, an important water ...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-14-124
更新日期：2014-05-13 00:00:00
abstract：BACKGROUND:Increasing age, several co-morbidities, environmental contamination, antibiotic exposure and other intestinal perturbations appear to be the greatest risk factors for C. difficile infection (CDI). Therefore, elderly care home residents are considered particularly vulnerable to the infection. The main objecti...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-016-0848-7
更新日期：2016-10-01 00:00:00
abstract：BACKGROUND:The human gastrointestinal (GI) tract microbiota has been a subject of intense research throughout the 3rd Millennium. Now that a general picture about microbiota composition in health and disease is emerging, questions about factors determining development of microbiotas with specific community structures w...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-019-1538-z
更新日期：2019-07-18 00:00:00
abstract：BACKGROUND:Bacteria adapted to live within animals can protect their hosts against harmful infections. Beyond antagonism with pathogens, a 'defensive' bacterial symbiont could engage in additional interactions with other colonizing micro-organisms. A single bacterium might thus have cascading ecological impacts on the ...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-020-01845-0
更新日期：2020-06-15 00:00:00
abstract：BACKGROUND:The aminoglycoside antibiotic gentamicin was supposed to induce a crosstalk between the Cpx- and the Arc-two-component systems (TCS). Here, we investigated the physical interaction of the respective TCS components and compared the results with their respective gene expression and protein abundance. The findi...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-017-1100-9
更新日期：2017-09-18 00:00:00
abstract：BACKGROUND:The 19 kDa lipoprotein of Mycobacterium tuberculosis (MTB) is an important target of the innate immune response. To investigate the effect of post-translation modification of this protein on innate recognition in the context of the whole bacillus, we derived a recombinant M. tuberculosis H37Rv that lacked th...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-9-93
更新日期：2009-05-14 00:00:00
abstract：BACKGROUND:Botrytis cinerea secretes a high number of proteins that are predicted to have numerous O-glycosylation sites, frequently grouped in highly O-glycosylated regions, and analysis of mutants affected in O-glycosylation has shown, in B. cinerea and in other phytopathogenic fungi, that this process is important f...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-014-0254-y
更新日期：2014-10-12 00:00:00
abstract：BACKGROUND:Atherosclerosis is still the leading cause of death in the western world. Besides known risk factors studies demonstrating Chlamydophila pneumoniae (C. pneumoniae) to be implicated in the progression of the disease, little is known about C. pneumoniae infection dynamics. We investigated whether C. pneumoniae...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-8-32
更新日期：2008-02-19 00:00:00
abstract：BACKGROUND:Several plant-pathogenic bacteria are transmitted by insect vector species that often also act as hosts. In this interface, these bacteria encounter plant endophytic, insect endosymbiotic and other microbes. Here, we used high throughput sequencing to examine the bacterial communities of five different psyll...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-020-01895-4
更新日期：2020-07-20 00:00:00
abstract：BACKGROUND:Bifidobacteria is one of the major gut commensal groups found in infants. Their colonization is commonly associated with beneficial effects to the host through mechanisms like niche occupation and nutrient competition against pathogenic bacteria. Iron is an essential element necessary for most microorganisms...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-014-0334-z
更新日期：2015-01-16 00:00:00
abstract：BACKGROUND:Streptococcus agalactiae (GBS) is a common pathogen to infect newborn, woman, the elderly, and immuno-compromised human and fish. 37 fish isolates and 554 human isolates of the GBS in 2007-2012 were investigated in serotypes, antibiotic susceptibility, genetic difference and pathogenicity to tilapia. RESULT...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-016-0794-4
更新日期：2016-08-02 00:00:00
abstract：BACKGROUND:Glycopeptidolipids (GPLs) are among the major free glycolipid components of the outer membrane of several saprophytic and clinically-relevant Mycobacterium species. The architecture of GPLs is based on a constant tripeptide-amino alcohol core of nonribosomal peptide synthetase origin that is N-acylated with ...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-12-118
更新日期：2012-06-22 00:00:00
abstract：BACKGROUND:The evolution of mycoplasmas from a common ancestor with Firmicutes has been characterized not only by genome down-sizing but also by horizontal gene transfer between mycoplasma species sharing a common host. The mechanisms of these gene transfers remain unclear because our knowledge of the mycoplasma mobile...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-12-257
更新日期：2012-11-12 00:00:00
abstract：BACKGROUND:Candida albicans yeast produces 10 distinct secreted aspartic proteases (Saps), which are some of the most important virulence factors of this pathogenic fungus. One of the suggested roles of Saps is their deregulating effect on various proteolytic cascades that constitute the major homeostatic systems in hu...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-015-0394-8
更新日期：2015-03-04 00:00:00
abstract：BACKGROUND:Heterologous prime-boost immunization protocols using different gene expression systems have proven to be successful tools in protecting against various diseases in experimental animal models. The main reason for using this approach is to exploit the ability of expression cassettes to prime or boost the immu...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-5-45
更新日期：2005-08-02 00:00:00
abstract：BACKGROUND:Vibrio parahaemolyticus is a food-borne pathogen causing inflammation of the gastrointestinal epithelium. Pathogenic strains of this bacterium possess two Type III Secretion Systems (TTSS) that deliver effector proteins into host cells. In order to better understand human host cell responses to V. parahaemol...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-10-329
更新日期：2010-12-30 00:00:00
abstract：BACKGROUND:A growing body of evidence suggests that Mycobacterium tuberculosis (Mtb) uses the host's cholesterol as a source of carbon and energy during infection. Strains defective in cholesterol transport or degradation exhibit attenuated growth in activated macrophages and diminished infectivity in animal models. Th...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-13-43
更新日期：2013-02-20 00:00:00
abstract：BACKGROUND:Single stranded ribonucleic acid (ssRNA) binds to toll-like receptor (TLR)7 leading to recruitment of immune cells and production of pro-inflammatory cytokines, which has been shown in mammals. In chickens, synthetic ssRNA analog, resiquimod, has been shown to elicit antiviral response against infectious bur...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-019-1398-6
更新日期：2019-02-08 00:00:00
abstract：BACKGROUND:Quorum sensing is a communication system that regulates gene expression in response to population density and often regulates virulence determinants. Deletion of the luxR homologue vjbR highly attenuates intracellular survival of Brucella melitensis and has been interpreted to be an indication of a role for ...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-10-167
更新日期：2010-06-08 00:00:00
abstract：BACKGROUND:Probiotics are increasingly applied to prevent and treat a range of infectious, immune related and gastrointestinal diseases. Despite this, the mechanisms behind the putative effects of probiotics are poorly understood. One of the suggested modes of probiotic action is modulation of the endogenous gut microb...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-017-1090-7
更新日期：2017-08-17 00:00:00
abstract：BACKGROUND:Enteropathogenic Escherichia coli (EPEC) are classified into typical and atypical strains based on the presence of the E. coli adherence factor (EAF) plasmid. The EAF plasmid contains the bfp (bundle-forming pilus) operon and the perABC (plasmid encoded regulator) gene cluster. A 1-kb cryptic region of EAF p...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-015-0539-9
更新日期：2015-10-05 00:00:00
abstract：BACKGROUND:Streptococcus pneumoniae is a common respiratory pathogen and a major causative agent of respiratory infections, including otitis media (OM). Pneumococcal biofilms have been demonstrated on biopsies of the middle ear mucosa in children receiving tympanostomy tubes, supporting the hypothesis that chronic OM m...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-8-173
更新日期：2008-10-08 00:00:00
abstract：BACKGROUND:Listeria monocytogenes is the causative agent of listeriosis, a serious disease affecting both animals and humans. Here, multilocus sequence typing (MLST) was used to characterize the genetic diversity of Listeria monocytogenes strains isolated from the natural environment and animal clinical cases in Europe...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-019-1676-3
更新日期：2019-12-18 00:00:00
