Abstract:
BACKGROUND:Streptococcus pneumoniae is a common respiratory pathogen and a major causative agent of respiratory infections, including otitis media (OM). Pneumococcal biofilms have been demonstrated on biopsies of the middle ear mucosa in children receiving tympanostomy tubes, supporting the hypothesis that chronic OM may involve biofilm development by pathogenic bacteria as part of the infectious process. To better understand pneumococcal biofilm formation six low-passage encapsulated nasopharyngeal isolates of S. pneumoniae were assessed over a six-eight day period in vitro. RESULTS:Multiparametric analysis divided the strains into two groups. Those with a high biofilm forming index (BFI) were structurally complex, exhibited greater lectin colocalization and were more resistant to azithromycin. Those with a low BFI developed less extensive biofilms and were more susceptible to azithromycin. dsDNA was present in the S. pneumoniae biofilm matrix in all strains and treatment with DNase I significantly reduced biofilm biomass. Since capsule expression has been hypothesized to be associated with decreased biofilm development, we also examined expression of cpsA, the first gene in the pneumococcal capsule operon. Interestingly, cpsA was downregulated in biofilms in both high and low BFI strains. CONCLUSION:All pneumococcal strains developed biofilms that exhibited extracellular dsDNA in the biofilm matrix, however strains with a high BFI correlated with greater carbohydrate-associated structural complexity and antibiotic resistance. Furthermore, all strains of S. pneumoniae showed downregulation of the cpsA gene during biofilm growth compared to planktonic culture, regardless of BFI ranking, suggesting downregulation of capsule expression occurs generally during adherent growth.
journal_name
BMC Microbioljournal_title
BMC microbiologyauthors
Hall-Stoodley L,Nistico L,Sambanthamoorthy K,Dice B,Nguyen D,Mershon WJ,Johnson C,Hu FZ,Stoodley P,Ehrlich GD,Post JCdoi
10.1186/1471-2180-8-173subject
Has Abstractpub_date
2008-10-08 00:00:00pages
173issn
1471-2180pii
1471-2180-8-173journal_volume
8pub_type
杂志文章相关文献
BMC MICROBIOLOGY文献大全abstract:BACKGROUND:Pectobacterium carotovorum subsp. carotovorum is a phytopathogenic enterobacterium responsible for soft rot, a disease characterized by extensive maceration of the affected plant tissue. This species also produces two or more antibacterial substances called bacteriocins, which enhance its competitiveness aga...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-181
更新日期:2009-08-27 00:00:00
abstract:BACKGROUND:Recent studies suggest that the reported protective effects of statins (HMG-CoA reductase inhibitors) against community-acquired pneumonia (CAP) and sepsis in humans may be due to confounders and a healthy user-effect. To directly test whether statins are protective against Streptococcus pneumoniae, the lead...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-73
更新日期:2012-05-15 00:00:00
abstract:BACKGROUND:Several intracellular bacterial pathogens have evolved subtle strategies to subvert vesicular trafficking pathways of their host cells to avoid killing and to replicate inside the cells. Brucellae are Gram-negative facultative intracellular bacteria that are responsible for brucellosis, a worldwide extended ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-014-0223-5
更新日期:2014-09-02 00:00:00
abstract:BACKGROUND:Quorum sensing is a communication system that regulates gene expression in response to population density and often regulates virulence determinants. Deletion of the luxR homologue vjbR highly attenuates intracellular survival of Brucella melitensis and has been interpreted to be an indication of a role for ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-167
更新日期:2010-06-08 00:00:00
abstract:BACKGROUND:Although the most widely accepted mechanism of action for polymyxins is related to bacterial lysis via disruption, we hypothesized that this antimicrobial drug class could have other effects on Pseudomonas aeruginosa planktonic and sessile cells. Little is known regarding oxidative burst and zeta potential (...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1485-8
更新日期:2019-05-29 00:00:00
abstract:BACKGROUND:Acetic acid has been used to clean and disinfect surfaces in the household for many decades. The antimicrobial efficacy of cleaning procedures can be considered particularly important for young, old, pregnant, immunocompromised people, but may also concern other groups, particularly with regards to the COVID...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01948-8
更新日期:2020-08-26 00:00:00
abstract:BACKGROUND:Antimicrobial helper-compounds may reverse antimicrobial resistance. Sertraline, a antidepressant drug, has been suggested as a tetracycline helper-compound. Tetracycline is the preferred antimicrobial for treatment of enteric diseases in pigs. This study is the first to evaluate the potency of sertraline as...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1383-5
更新日期:2019-01-11 00:00:00
abstract:BACKGROUND:Campylobacter jejuni (C. jejuni) is a leading cause of foodborne gastroenteritis worldwide. This bacterium lacks many of the classical virulence factors, and flagellum-associated persistent colonization has been shown to be crucial for its pathogenesis. The flagellum plays a multifunctional role in C. jejuni...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1318-1
更新日期:2018-10-22 00:00:00
abstract:BACKGROUND:Leptospires lack many of the homologs for oxidative defense present in other bacteria, but do encode homologs of the Bacteriodes aerotolerance (Bat) proteins, which have been proposed to fulfill this function. Bat homologs have been identified in all families of the phylum Spirochaetes, yet a specific functi...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-290
更新日期:2012-12-13 00:00:00
abstract:BACKGROUND:Emergence of drug resistant varieties of tuberculosis is posing a major threat to global tuberculosis eradication programmes. Although several approaches have been explored to counter resistance, there has been limited success due to a lack of understanding of how resistance emerges in bacteria upon drug tre...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-234
更新日期:2008-12-23 00:00:00
abstract:BACKGROUND:Poorly understood but highly diverse microbial communities exist within anoxic and oxygen-depleted marine sediments. These communities often harbour single-celled eukaryotes that form symbiotic associations with different prokaryotes. During low tides in South-western British Columbia, Canada, vast areas of ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-145
更新日期:2010-05-19 00:00:00
abstract:BACKGROUND:Acinetobacter baumannii is a gram-negative bacterium which causes opportunistic infections in immunocompromised hosts. Genome plasticity has given rise to a wide range of strain variation with respect to antimicrobial resistance profiles and expression of virulence factors which lead to altered phenotypes as...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-02082-1
更新日期:2021-01-09 00:00:00
abstract:BACKGROUND:The KdpD/KdpE two-component system of Escherichia coli regulates expression of the kdpFABC operon encoding the high affinity K+ transport system KdpFABC. The input domain of KdpD comprises a domain that belongs to the family of universal stress proteins (Usp). It has been previously demonstrated that UspC bi...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-133
更新日期:2009-07-09 00:00:00
abstract:BACKGROUND:Candida krusei has been known to exhibit communal interactions such as pellicle formation and crawling out of nutritional broth. We noticed another possible interaction on agar surfaces, where C. krusei yeast cells formed mycelia along agar surfaces toward each other. We report here the results of experiment...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-0972-z
更新日期:2017-03-11 00:00:00
abstract:BACKGROUND:Bovicin HC5 is an antimicrobial peptide that shows a broad spectrum of activity and potential for biotechnological and therapeutic applications. To gain insight about the safety of bovicin HC5 application, the histological and immunostimulatory effects of orally administrated bovicin HC5 to BALB/c mice were ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-69
更新日期:2013-03-27 00:00:00
abstract:BACKGROUND:Predicting antibiotic resistance before it emerges at clinical settings constitutes a novel approach for preventing and fighting resistance of bacterial pathogens. To analyse the possibility that novel plasmid-encoded quinolone resistance determinants (Qnr) can emerge and disseminate among bacterial pathogen...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-148
更新日期:2008-09-16 00:00:00
abstract:BACKGROUND:The Gram-negative phytopathogenic bacterium Xanthomonas campestris pv. campestris recruits the hrp/T3SS system to inject pathogenicity effector proteins into host cells and uses the rpf/DSF cell-cell signaling system to regulate the expression of virulence factors such as extracellular enzymes and polysaccha...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1233-5
更新日期:2018-09-03 00:00:00
abstract:BACKGROUND:Ochrobactrum anthropi is a versatile bacterial species with strains living in very diverse habitats. It is increasingly recognized as opportunistic pathogen in hospitalized patients. The population biology of the species particularly with regard to the characteristics of the human isolates is being investiga...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-267
更新日期:2009-12-18 00:00:00
abstract:BACKGROUND:Affecting the core functional microbiome, peculiar high level taxonomic unbalances of the human intestinal microbiota have been recently associated with specific diseases, such as obesity, inflammatory bowel diseases, and intestinal inflammation. RESULTS:In order to specifically monitor microbiota unbalance...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-116
更新日期:2010-04-19 00:00:00
abstract:BACKGROUND:Since bacteria embedded in biofilms are far more difficult to eradicate than planktonic infections, it would be useful to know whether certain Staphylococcus aureus lineages are especially involved in strong biofilm formation. For this reason, in vitro biofilm formation of 228 clinical S. aureus isolates of ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-229
更新日期:2009-10-28 00:00:00
abstract:BACKGROUND:Streptococcus suis is a major swine pathogen worldwide that causes meningitis, septicemia, arthritis, and endocarditis. Using animal models, a surface-associated subtilisin-like protease (SspA) has recently been shown to be an important virulence factor for S. suis. In this study, we hypothesized that the S....
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-11-47
更新日期:2011-03-01 00:00:00
abstract:BACKGROUND:The mce operons play an important role in the entry of M. tuberculosis into macrophages and non-phagocytic cells. Their non-redundant function as well as complex regulation is implied by the phenotype of mce mutants. Recently, mce1 operon was found to extend over 13 genes, fadD5 (Rv0166) being the first gene...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-128
更新日期:2010-04-27 00:00:00
abstract:BACKGROUND:Mass-rearing, domestication and gamma irradiation of tephritid fruit flies used in sterile insect technique (SIT) programmes can negatively impact fly quality and performance. Symbiotic bacteria supplied as probiotics to mass-reared fruit flies may help to overcome some of these issues. However, the effects ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1649-6
更新日期:2019-12-24 00:00:00
abstract:BACKGROUND:Candida parapsilosis typically is a commensal of human skin. However, when host immune defense is compromised or the normal microflora balance is disrupted, C. parapsilosis transforms itself into an opportunistic pathogen. Candida-derived lipase has been identified as potential virulence factor. Even though ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-11-122
更新日期:2011-05-29 00:00:00
abstract:BACKGROUND:Lactobacillus species are used as probiotics and play an important role in fermented food production. However, use of 16S rRNA gene sequences as standard markers for the differentiation of Lactobacillus species offers a very limited scope, as several species of Lactobacillus share similar 16S rRNA gene seque...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01781-z
更新日期:2020-04-15 00:00:00
abstract:BACKGROUND:Burkholderia pseudomallei is the etiological agent of melioidosis and a CDC category B select agent with no available effective vaccine. Previous immunizations in mice have utilized the lipopolysaccharide (LPS) as a potential vaccine target because it is known as one of the most important antigenic epitopes ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-250
更新日期:2012-11-05 00:00:00
abstract:BACKGROUND:Chlortetracycline (CTC) is one of the commercially important tetracyclines (TCs) family product and is mainly produced by Streptomyces. CTC is still in a great demand due to its broad-spectrum activity against pathogens. Engineering transcriptional control allows the cell to allocate its valuable resources t...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1670-9
更新日期:2019-12-10 00:00:00
abstract:BACKGROUND:Modifiable lifestyle factors (e.g. dietary intake and physical activity) are important contributors to weight gain during college. The purpose of this study was to evaluate whether associations exist between body mass index, physical activity, screen time, dietary consumption (fat, protein, carbohydrates, an...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1362-x
更新日期:2018-12-12 00:00:00
abstract:BACKGROUND:The Rio Grande River is the natural boundary between U.S. and Mexico from El Paso, TX to Brownsville, TX. and is one of the major water resources of the area. Agriculture, farming, maquiladora industry, domestic activities, as well as differences in disposal regulations and enforcement increase the contamina...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-4-17
更新日期:2004-04-22 00:00:00
abstract:BACKGROUND:The aim of this study was to identify mutations of rpoB, katG, inhA and ahp-genes associated Mycobacterium tuberculosis resistance to rifampicin (RIF) and isoniazid (INH) in Kyrgyz Republic. We studied 633 smear samples from the primary pulmonary tuberculosis (TB) patients. We verified Mycobacterium tubercul...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1168-x
更新日期:2018-03-22 00:00:00