Abstract：
BACKGROUND:Candida albicans yeast produces 10 distinct secreted aspartic proteases (Saps), which are some of the most important virulence factors of this pathogenic fungus. One of the suggested roles of Saps is their deregulating effect on various proteolytic cascades that constitute the major homeostatic systems in human hosts, including blood coagulation, fibrinolysis, and kallikrein-kinin systems. This study compared the characteristics of the action of all 10 Saps on human kininogens, which results in generating proinflammatory bradykinin-related peptides (kinins). RESULTS:Recombinant forms of Saps, heterologously overexpressed in Pichia pastoris were applied. Except for Sap7 and Sap10, all Saps effectively cleaved the kininogens, with the highest hydrolytic activity toward the low-molecular-mass form (LK). Sap1-6 and 8 produced a biologically active kinin-Met-Lys-bradykinin-and Sap3 was exceptional in terms of the kinin-releasing yield (>60% LK at pH 5.0 after 24 hours). Des-Arg(1)-bradykinin was released from LK by Sap9 at a comparably high yield, but this peptide was assumed to be biologically inactive because it was unable to interact with cellular B2-type kinin receptors. However, the collaborative actions of Sap9 and Sap1, -2, -4-6, and -8 on LK rerouted kininogen cleavage toward the high-yield release of the biologically active Met-Lys-bradykinin. CONCLUSIONS:Our present results, together with the available data on the expression of individual SAP genes in candidal infection models, suggest a biological potential of Saps to produce kinins at the infection foci. The kinin release during candidiasis can involve predominant and complementary contributions of two different Sap3- and Sap9-dependent mechanisms.
journal_name
BMC Microbioljournal_title
BMC microbiologyauthors
Kozik A,Gogol M,Bochenska O,Karkowska-Kuleta J,Wolak N,Kamysz W,Aoki W,Ueda M,Faussner A,Rapala-Kozik Mdoi
10.1186/s12866-015-0394-8subject
Has Abstractpub_date
2015-03-04 00:00:00pages
60issn
1471-2180pii
10.1186/s12866-015-0394-8journal_volume
15pub_type
杂志文章相关文献
BMC MICROBIOLOGY文献大全abstract：BACKGROUND:Nitrosomonas europaea is a widely studied chemolithoautotrophic ammonia oxidizing bacterium. While significant work exists on the ammonia oxidation pathway of N. europaea, its responses to factors such as dissolved oxygen limitation or sufficiency or exposure to high nitrite concentrations, particularly at t...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-10-70
更新日期：2010-03-04 00:00:00
abstract：BACKGROUND:Bacterial nucleoid consists of genome DNA, RNA, and hundreds of nucleoid-associated proteins (NAPs). Escherichia coli nucleoid is compacted towards the stationary phase, replacing most log-phase NAPs with the major stationary-phase nucleoid protein, Dps. In contrast, Staphylococcus aureus nucleoid sustains t...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-017-1114-3
更新日期：2017-10-02 00:00:00
abstract：BACKGROUND:The phenomenon of misdiagnosing tuberculosis (TB) by laboratory cross-contamination when culturing Mycobacterium tuberculosis (MTB) has been widely reported and it has an obvious clinical, therapeutic and social impact. The final confirmation of a cross-contamination event requires the molecular identificati...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-8-30
更新日期：2008-02-14 00:00:00
abstract：BACKGROUND:Classical bacteriological characteristics of Salmonella enterica indicate that the members of this species are unable to utilize lactose as a carbon source. However, lactose-fermenting (Lac+) strains of several Salmonella serovars have been isolated from different foodborne outbreaks as well as different geo...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-015-0511-8
更新日期：2015-08-25 00:00:00
abstract：BACKGROUND:A biofilm is a complex community of microorganisms that develop on surfaces in diverse environments. The thickness of the biofilm plays a crucial role in the physiology of the immobilized bacteria. The most cariogenic bacteria, mutans streptococci, are common inhabitants of a dental biofilm community. In thi...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-8-236
更新日期：2008-12-29 00:00:00
abstract：BACKGROUND:The use of chemical fungicides against fungal pathogens adversely affects soil and plant health thereby resulting in overall environmental hazards. Therefore, biological source for obtaining antifungal agents is considered as an environment-friendly alternative for controlling fungal pathogens. RESULTS:In t...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-019-1440-8
更新日期：2019-04-02 00:00:00
abstract：BACKGROUND:Mycobacterium avium is an environmental mycobacterium that can be divided into the subspecies avium, hominissuis, paratuberculosis and silvaticum. Some M. avium subspecies are opportunistic pathogens for animals and humans. They are ubiquitous in nature and can be isolated from natural sources of water, soil...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-7-14
更新日期：2007-03-05 00:00:00
abstract：BACKGROUND:"Candidatus Phytoplasma aurantifolia", is the causative agent of witches' broom disease in Mexican lime trees (Citrus aurantifolia L.), and is responsible for major losses of Mexican lime trees in Southern Iran and Oman. The pathogen is strictly biotrophic, and thus is completely dependent on living host cel...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-11-1
更新日期：2011-01-01 00:00:00
abstract：BACKGROUND:The genus Phytophthora includes a group of agriculturally important pathogens and they are commonly regarded as water molds. They produce motile zoospores that can move via water currents and on their own locomotion in aquatic environments. However, zoosporic response to dissolved oxygen, an important water ...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-14-124
更新日期：2014-05-13 00:00:00
abstract：BACKGROUND:The skin microbiome serves as a first line defense against pathogens in vertebrates. In amphibians, it has the potential to protect against the chytrid fungus Batrachochytrium dendrobatis (Bd), a likely agent of amphibian declines. Alteration of the microbiome associated with unfavorable environmental change...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-020-01979-1
更新日期：2020-09-22 00:00:00
abstract：BACKGROUND:Most of the studies evaluating the secular trends of blood isolates come from tertiary hospitals in urban areas. We sought to study the trends of the antimicrobial resistance of blood isolates in patients from a rural population hospitalized in a tertiary hospital in a small city in Greece. METHODS:We retro...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-6-41
更新日期：2006-05-02 00:00:00
abstract：BACKGROUND:The human gastrointestinal (GI) tract microbiota is characterised by an abundance of uncultured bacteria most often assigned in phyla Firmicutes and Bacteroidetes. Diversity of this microbiota, even though approached with culture independent techniques in several studies, still requires more elucidation. The...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-9-68
更新日期：2009-04-08 00:00:00
abstract：BACKGROUND:Identification and characterization of intervening sequences (IVSs) within 23S rRNA genes from Campylobacter organisms including atypical campylobacters were carried out using two PCR primer pairs, designed to generate helix 25 and 45 regions. RESULTS:Only C. sputorum biovar sputorum LMG7975 and fecalis LMG...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-9-256
更新日期：2009-12-11 00:00:00
abstract：BACKGROUND:Recent studies suggest that the reported protective effects of statins (HMG-CoA reductase inhibitors) against community-acquired pneumonia (CAP) and sepsis in humans may be due to confounders and a healthy user-effect. To directly test whether statins are protective against Streptococcus pneumoniae, the lead...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-12-73
更新日期：2012-05-15 00:00:00
abstract：BACKGROUND:Biofilm formation by Candida albicans has shown to be highly variable and is directly associated with pathogenicity and poor clinical outcomes in patients at risk. The aim of this study was to test the hypotheses that the extracellular DNA release by C. albicans is strain dependent and is associated with bio...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-014-0303-6
更新日期：2014-12-05 00:00:00
abstract：BACKGROUND:Quorum sensing is a communication system that regulates gene expression in response to population density and often regulates virulence determinants. Deletion of the luxR homologue vjbR highly attenuates intracellular survival of Brucella melitensis and has been interpreted to be an indication of a role for ...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-10-167
更新日期：2010-06-08 00:00:00
abstract：BACKGROUND:The lung of patients with cystic fibrosis (CF) is particularly sensitive to Pseudomonas aeruginosa. This bacterium plays an important role in the poor outcome of CF patients. During the disease progress, first acquisition of P. aeruginosa is the key-step in the management of CF patients. Quantitative PCR (qP...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-13-143
更新日期：2013-06-21 00:00:00
abstract：BACKGROUND:The Bacillus anthracis S-layer protein, BslA, plays a crucial role in mammalian infection. BslA is required to mediate adherence between host cells and vegetative forms of bacteria and this interaction promotes target organs adherence and blood-brain barrier (BBB) penetration in vivo. This study attempts to ...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-016-0802-8
更新日期：2016-08-11 00:00:00
abstract：BACKGROUND:Candida parapsilosis typically is a commensal of human skin. However, when host immune defense is compromised or the normal microflora balance is disrupted, C. parapsilosis transforms itself into an opportunistic pathogen. Candida-derived lipase has been identified as potential virulence factor. Even though ...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-11-122
更新日期：2011-05-29 00:00:00
abstract：BACKGROUND:Hypervirulent K. pneumoniae (hvKp) causes severe community acquired infections, predominantly in Asia. Though initially isolated from liver abscesses, they are now prevalent among invasive infections such as bacteraemia. There have been no studies reported till date on the prevalence and characterisation of ...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-017-1148-6
更新日期：2018-01-08 00:00:00
abstract：BACKGROUND:Next-generation sequencing platforms have revolutionised our ability to investigate the microbiota composition of complex environments, frequently through 16S rRNA gene sequencing of the bacterial component of the community. Numerous factors, including DNA extraction method, primer sequences and sequencing p...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-016-0738-z
更新日期：2016-06-24 00:00:00
abstract：BACKGROUND:Enterohemorrhagic Escherichia coli (EHEC) colonizes the intestinal epithelium and causes attaching and effacing (A/E) lesions. Expression of virulence genes, particularly those from the locus of the enterocyte effacement (LEE) pathogenicity island is required for the formation of a type three secretion syste...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-12-231
更新日期：2012-10-11 00:00:00
abstract：BACKGROUND:The Gram-negative phytopathogenic bacterium Xanthomonas campestris pv. campestris recruits the hrp/T3SS system to inject pathogenicity effector proteins into host cells and uses the rpf/DSF cell-cell signaling system to regulate the expression of virulence factors such as extracellular enzymes and polysaccha...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-018-1233-5
更新日期：2018-09-03 00:00:00
abstract：BACKGROUND:It is unclear how DNA is packaged in a bacterial cell in the absence of nucleosomes. To investigate the initial level of DNA condensation in bacterial nucleoid we used in vivo DNA digestion coupled with high-throughput sequencing of the digestion-resistant fragments. To this end, we transformed E. coli cells...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-016-0637-3
更新日期：2016-02-20 00:00:00
abstract：BACKGROUND:The development of vaccines and evaluation of novel treatment strategies for invasive group A streptococcal (iGAS) disease requires suitable models of human infection that can be monitored longitudinally and are preferably non-invasive. Bio-photonic imaging provides an opportunity to reduce use of animals in...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-018-1200-1
更新日期：2018-06-19 00:00:00
abstract：BACKGROUND:Bifidobacteria is one of the major gut commensal groups found in infants. Their colonization is commonly associated with beneficial effects to the host through mechanisms like niche occupation and nutrient competition against pathogenic bacteria. Iron is an essential element necessary for most microorganisms...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-014-0334-z
更新日期：2015-01-16 00:00:00
abstract：BACKGROUND:Hepatitis C virus (HCV) core protein and nonstructural protein 4B (NS4B) are potentially oncogenic. Aberrant activation of the Wnt/β-catenin signaling pathway is closely associated with hepatocarcinogenesis. We investigated the effects of HCV type 1b core protein and NS4B on Wnt/β-catenin signaling in variou...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-017-1032-4
更新日期：2017-05-25 00:00:00
abstract：BACKGROUND:Edwardsiella ictaluri is a Gram-negative facultative intracellular anaerobe and the etiologic agent of enteric septicemia of channel catfish (ESC). To the catfish industry, ESC is a devastating disease due to production losses and treatment costs. Identification of virulence mechanisms of E. ictaluri is crit...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-019-1429-3
更新日期：2019-03-08 00:00:00
abstract：BACKGROUND:Thermotolerant Campylobacter is among the more prevalent bacterial pathogens that cause foodborne diseases. This study aimed at evaluating the occurrence of thermotolerant Campylobacter contamination in chicken carcasses and processing plant stations (chilling water, scalding water, defeathering machinery, e...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-9-94
更新日期：2009-05-15 00:00:00
abstract：BACKGROUND:The ability of an intracellular pathogen to establish infection depends on the capacity of the organism to survive and replicate inside the host. Mycobacterium fortuitum is a bacteria that contains genes involved in the detoxification of the oxygen reactive species such as those produced by the host during t...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-8-100
更新日期：2008-06-19 00:00:00
