Modelling invasive group A streptococcal disease using bioluminescence.

Abstract:

BACKGROUND:The development of vaccines and evaluation of novel treatment strategies for invasive group A streptococcal (iGAS) disease requires suitable models of human infection that can be monitored longitudinally and are preferably non-invasive. Bio-photonic imaging provides an opportunity to reduce use of animals in infection modelling and refine the information that can be obtained, however the range of bioluminescent GAS strains available is limited. In this study we set out to develop bioluminescent iGAS strains for use in in vivo pneumonia and soft tissue disease models. RESULTS:Using clinical emm1, emm3, and emm89 GAS strains that were transformed with constructs carrying the luxABCDE operon, growth and bioluminescence of transformed strains were characterised in vitro and in vivo. Emm3 and emm89 strains expressed detectable bioluminescence when transformed with a replicating plasmid and light production correlated with viable bacterial counts in vitro, however plasmid instability precluded use in the absence of antimicrobial pressure. Emm89 GAS transformed with an integrating construct demonstrated stable bioluminescence that was maintained in the absence of antibiotics. Bioluminescence of the emm89 strain correlated with viable bacterial counts both in vitro and immediately following infection in vivo. Although bioluminescence conferred a detectable fitness burden to the emm89 strain during soft tissue infection in vivo, it did not prevent dissemination to distant tissues. CONCLUSION:Development of stably bioluminescent GAS for use in vitro and in vivo models of infection should facilitate development of novel therapeutics and vaccines while also increasing our understanding of infection progression and transmission routes.

journal_name

BMC Microbiol

journal_title

BMC microbiology

authors

Lamb LE,Zhi X,Alam F,Pyzio M,Scudamore CL,Wiles S,Sriskandan S

doi

10.1186/s12866-018-1200-1

subject

Has Abstract

pub_date

2018-06-19 00:00:00

pages

60

issue

1

issn

1471-2180

pii

10.1186/s12866-018-1200-1

journal_volume

18

pub_type

杂志文章
  • Genotyping of Chlamydophila psittaci using a new DNA microarray assay based on sequence analysis of ompA genes.

    abstract:BACKGROUND:The currently used genotyping system for the avian zoonotic pathogen Chlamydophila (C.) psittaci has evolved from serology and is based on ompA sequence variations. It includes seven avian and two non-avian genotypes. Restriction enzyme cleavage of the amplified ompA gene and, less frequently, ompA sequencin...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-63

    authors: Sachse K,Laroucau K,Hotzel H,Schubert E,Ehricht R,Slickers P

    更新日期:2008-04-17 00:00:00

  • Repertoire of the gut microbiota from stomach to colon using culturomics and next-generation sequencing.

    abstract:BACKGROUND:Most studies on the human microbiota have analyzed stool samples, although a large proportion of the absorption of nutrients takes place in upper gut tract. We collected samples from different locations along the entire gastrointestinal tract from six patients who had simultaneously undergone upper endoscopy...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1304-7

    authors: Mailhe M,Ricaboni D,Vitton V,Gonzalez JM,Bachar D,Dubourg G,Cadoret F,Robert C,Delerce J,Levasseur A,Fournier PE,Angelakis E,Lagier JC,Raoult D

    更新日期:2018-10-24 00:00:00

  • Differences between predicted outer membrane proteins of genotype 1 and 2 Mannheimia haemolytica.

    abstract:BACKGROUND:Mannheimia haemolytica strains isolated from North American cattle have been classified into two genotypes (1 and 2). Although members of both genotypes have been isolated from the upper and lower respiratory tracts of cattle with or without bovine respiratory disease (BRD), genotype 2 strains are much more ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01932-2

    authors: Clawson ML,Schuller G,Dickey AM,Bono JL,Murray RW,Sweeney MT,Apley MD,DeDonder KD,Capik SF,Larson RL,Lubbers BV,White BJ,Blom J,Chitko-McKown CG,Brichta-Harhay DM,Smith TPL

    更新日期:2020-08-12 00:00:00

  • Campylobacter jejuni dsb gene expression is regulated by iron in a Fur-dependent manner and by a translational coupling mechanism.

    abstract:BACKGROUND:Many bacterial extracytoplasmic proteins are stabilized by intramolecular disulfide bridges that are formed post-translationally between their cysteine residues. This protein modification plays an important role in bacterial pathogenesis, and is facilitated by the Dsb (disulfide bond) family of the redox pro...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-11-166

    authors: Grabowska AD,Wandel MP,Łasica AM,Nesteruk M,Roszczenko P,Wyszyńska A,Godlewska R,Jagusztyn-Krynicka EK

    更新日期:2011-07-25 00:00:00

  • Characterization and anti-salmonella activities of lactic acid bacteria isolated from cattle faeces.

    abstract:BACKGROUND:Non typhoidal salmonellosis is one of the neglected zoonoses in most African countries. The use of sub-therapeutic doses of antibiotics as animal growth promoter enhances the emergence and dissemination of antimicrobial resistance in bacteria with food animal reservoirs and may also results in antibiotics re...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1248-y

    authors: Adetoye A,Pinloche E,Adeniyi BA,Ayeni FA

    更新日期:2018-08-30 00:00:00

  • Diversity and transcription of proteases involved in the maturation of hydrogenases in Nostoc punctiforme ATCC 29133 and Nostoc sp. strain PCC 7120.

    abstract:BACKGROUND:The last step in the maturation process of the large subunit of [NiFe]-hydrogenases is a proteolytic cleavage of the C-terminal by a hydrogenase specific protease. Contrary to other accessory proteins these hydrogenase proteases are believed to be specific whereby one type of hydrogenases specific protease o...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-53

    authors: Devine E,Holmqvist M,Stensjö K,Lindblad P

    更新日期:2009-03-11 00:00:00

  • The role of the two-component systems Cpx and Arc in protein alterations upon gentamicin treatment in Escherichia coli.

    abstract:BACKGROUND:The aminoglycoside antibiotic gentamicin was supposed to induce a crosstalk between the Cpx- and the Arc-two-component systems (TCS). Here, we investigated the physical interaction of the respective TCS components and compared the results with their respective gene expression and protein abundance. The findi...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-017-1100-9

    authors: Ćudić E,Surmann K,Panasia G,Hammer E,Hunke S

    更新日期:2017-09-18 00:00:00

  • Heat shock cognate protein 70 contributes to Brucella invasion into trophoblast giant cells that cause infectious abortion.

    abstract:BACKGROUND:The cell tropism of Brucella abortus, a causative agent of brucellosis and facultative intracellular pathogen, in the placenta is thought to be a key event of infectious abortion, although the molecular mechanism for this is largely unknown. There is a higher degree of bacterial colonization in the placenta ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-212

    authors: Watanabe K,Tachibana M,Tanaka S,Furuoka H,Horiuchi M,Suzuki H,Watarai M

    更新日期:2008-12-05 00:00:00

  • Inhibition of HSP70 reduces porcine reproductive and respiratory syndrome virus replication in vitro.

    abstract:BACKGROUND:Successful viral infection requires the involvement of host cellular factors in their life cycle. Heat shock protein 70 (HSP70) can be recruited by numerous viruses to promote the folding, maturation, or assembly of viral proteins. We have previously shown that HSP70 is significantly elevated in porcine repr...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-14-64

    authors: Gao J,Xiao S,Liu X,Wang L,Ji Q,Mo D,Chen Y

    更新日期:2014-03-13 00:00:00

  • Wolbachia infection in Argentinean populations of Anastrepha fraterculus sp1: preliminary evidence of sex ratio distortion by one of two strains.

    abstract:BACKGROUND:Wolbachia, one of the most abundant taxa of intracellular Alphaproteobacteria, is widespread among arthropods and filarial nematodes. The presence of these maternally inherited bacteria is associated with modifications of host fitness, including a variety of reproductive abnormalities, such as cytoplasmic in...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1652-y

    authors: Conte CA,Segura DF,Milla FH,Augustinos A,Cladera JL,Bourtzis K,Lanzavecchia SB

    更新日期:2019-12-24 00:00:00

  • New probes used for IS1245 and IS1311 restriction fragment length polymorphism of Mycobacterium avium subsp. avium and Mycobacterium avium subsp. hominissuis isolates of human and animal origin in Norway.

    abstract:BACKGROUND:Mycobacterium avium is an environmental mycobacterium that can be divided into the subspecies avium, hominissuis, paratuberculosis and silvaticum. Some M. avium subspecies are opportunistic pathogens for animals and humans. They are ubiquitous in nature and can be isolated from natural sources of water, soil...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-7-14

    authors: Johansen TB,Olsen I,Jensen MR,Dahle UR,Holstad G,Djønne B

    更新日期:2007-03-05 00:00:00

  • Association of Bordetella dermonecrotic toxin with the extracellular matrix.

    abstract:BACKGROUND:Bordetella dermonecrotic toxin (DNT) causes the turbinate atrophy in swine atrophic rhinitis, caused by a Bordetella bronchiseptica infection of pigs, by inhibiting osteoblastic differentiation. The toxin is not actively secreted from the bacteria, and is presumed to be present in only small amounts in infec...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-247

    authors: Fukui-Miyazaki A,Kamitani S,Miyake M,Horiguchi Y

    更新日期:2010-09-25 00:00:00

  • Detection of Burkholderia pseudomallei O-antigen serotypes in near-neighbor species.

    abstract:BACKGROUND:Burkholderia pseudomallei is the etiological agent of melioidosis and a CDC category B select agent with no available effective vaccine. Previous immunizations in mice have utilized the lipopolysaccharide (LPS) as a potential vaccine target because it is known as one of the most important antigenic epitopes ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-12-250

    authors: Stone JK,Mayo M,Grasso SA,Ginther JL,Warrington SD,Allender CJ,Doyle A,Georgia S,Kaestli M,Broomall SM,Karavis MA,Insalaco JM,Hubbard KS,McNew LA,Gibbons HS,Currie BJ,Keim P,Tuanyok A

    更新日期:2012-11-05 00:00:00

  • Phenotypic and genotypic characterisation of multiple antibiotic-resistant Staphylococcus aureus exposed to subinhibitory levels of oxacillin and levofloxacin.

    abstract:BACKGROUND:The emergence and spread of multidrug resistant methicillin-resistant Staphylococcus aureus (MDR-MRSA) has serious health consequences in the presence of sub-MIC antibiotics. Therefore, this study was designed to evaluate β-lactamase activity, efflux activity, biofilm formation, and gene expression pattern i...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0791-7

    authors: Jo A,Ahn J

    更新日期:2016-07-29 00:00:00

  • Early detection of viable Francisella tularensis in environmental matrices by culture-based PCR.

    abstract:BACKGROUND:Francisella tularensis is a fastidious, Gram-negative coccobacillus and is the causative agent of tularemia. To assess viability yet overcome lengthy incubation periods, a culture-based PCR method was used to detect early growth of the lowest possible number of F. tularensis cells. This method utilized a pre...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01748-0

    authors: Buse HY,Morris BJ,Rice EW

    更新日期:2020-03-25 00:00:00

  • Mycobacterium abscessus multispacer sequence typing.

    abstract:BACKGROUND:Mycobacterium abscessus group includes antibiotic-resistant, opportunistic mycobacteria that are responsible for sporadic cases and outbreaks of cutaneous, pulmonary and disseminated infections. However, because of their close genetic relationships, accurate discrimination between the various strains of thes...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-3

    authors: Sassi M,Ben Kahla I,Drancourt M

    更新日期:2013-01-07 00:00:00

  • Functional significance of nuclear export and mRNA binding of meiotic regulator Spo5 in fission yeast.

    abstract:BACKGROUND:Meiotic cells undergo two rounds of nuclear division and generate gametes. Previous studies have indicated that a number of transcription factors modulate the transcriptome in successive waves during meiosis and spore formation in fission yeast. However, the mechanisms underlying the post-transcriptional reg...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-14-188

    authors: Togashi N,Yamashita A,Sato M,Yamamoto M

    更新日期:2014-07-15 00:00:00

  • CCL5 regulation of mucosal chlamydial immunity and infection.

    abstract:BACKGROUND:Following genital chlamydial infection, an early T helper type 1 (Th1)-associated immune response precedes the activation and recruitment of specific Th1 cells bearing distinct chemokine receptors, subsequently leading to the clearance of Chlamydia. We have shown that CCR5, a receptor for CCL5, is crucial fo...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-136

    authors: Sakthivel SK,Singh UP,Singh S,Taub DD,Igietseme JU,Lillard JW Jr

    更新日期:2008-08-13 00:00:00

  • Distribution and diversity of mycoplasma plasmids: lessons from cryptic genetic elements.

    abstract:BACKGROUND:The evolution of mycoplasmas from a common ancestor with Firmicutes has been characterized not only by genome down-sizing but also by horizontal gene transfer between mycoplasma species sharing a common host. The mechanisms of these gene transfers remain unclear because our knowledge of the mycoplasma mobile...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-12-257

    authors: Breton M,Tardy F,Dordet-Frisoni E,Sagne E,Mick V,Renaudin J,Sirand-Pugnet P,Citti C,Blanchard A

    更新日期:2012-11-12 00:00:00

  • Comparative genotyping and phenotyping of Aspergillus fumigatus isolates from humans, dogs and the environment.

    abstract:BACKGROUND:Aspergillus fumigatus is a ubiquitous saprotrophic fungus and an opportunistic pathogen of humans and animals. Humans and animals can inhale hundreds of A. fumigatus spores daily. Normally this is harmless for humans, but in case of immunodeficiency, invasive pulmonary aspergillosis (IPA) can develop with a ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1244-2

    authors: Valdes ID,van den Berg J,Haagsman A,Escobar N,Meis JF,Hagen F,Haas PJ,Houbraken J,Wösten HAB,de Cock H

    更新日期:2018-09-17 00:00:00

  • Microbial iron management mechanisms in extremely acidic environments: comparative genomics evidence for diversity and versatility.

    abstract:BACKGROUND:Iron is an essential nutrient but can be toxic at high intracellular concentrations and organisms have evolved tightly regulated mechanisms for iron uptake and homeostasis. Information on iron management mechanisms is available for organisms living at circumneutral pH. However, very little is known about how...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-203

    authors: Osorio H,Martínez V,Nieto PA,Holmes DS,Quatrini R

    更新日期:2008-11-24 00:00:00

  • Exposure to a social stressor disrupts the community structure of the colonic mucosa-associated microbiota.

    abstract:BACKGROUND:The microbiota of the mammalian gastrointestinal (GI) tract consists of diverse populations of commensal bacteria that interact with host physiological function. Dysregulating these populations, through exogenous means such as antibiotics or dietary changes, can have adverse consequences on the health of the...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-14-189

    authors: Galley JD,Nelson MC,Yu Z,Dowd SE,Walter J,Kumar PS,Lyte M,Bailey MT

    更新日期:2014-07-15 00:00:00

  • Identification of nucleoid associated proteins (NAPs) under oxidative stress in Staphylococcus aureus.

    abstract:BACKGROUND:Bacterial nucleoid consists of genome DNA, RNA, and hundreds of nucleoid-associated proteins (NAPs). Escherichia coli nucleoid is compacted towards the stationary phase, replacing most log-phase NAPs with the major stationary-phase nucleoid protein, Dps. In contrast, Staphylococcus aureus nucleoid sustains t...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-017-1114-3

    authors: Ushijima Y,Ohniwa RL,Morikawa K

    更新日期:2017-10-02 00:00:00

  • Genetic stability of Brucella abortus isolates from an outbreak by multiple-locus variable-number tandem repeat analysis (MLVA16).

    abstract:BACKGROUND:Brucellosis caused by Brucella abortus is one of the most important zoonoses in the world. Multiple-locus variable-number tandem repeat analysis (MLVA16) has been shown be a useful tool to epidemiological traceback studies in B. abortus infection. Thus, the present study aimed (i) to evaluate the genetic div...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-14-186

    authors: Dorneles EM,Santana JA,Alves TM,Pauletti RB,Mol JP,Heinemann MB,Lage AP

    更新日期:2014-07-11 00:00:00

  • Pseudoscorpion Wolbachia symbionts: diversity and evidence for a new supergroup S.

    abstract:BACKGROUND:Wolbachia are the most widely spread endosymbiotic bacteria, present in a wide variety of insects and two families of nematodes. As of now, however, relatively little genomic data has been available. The Wolbachia symbiont can be parasitic, as described for many arthropod systems, an obligate mutualist, as i...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01863-y

    authors: Lefoulon E,Clark T,Borveto F,Perriat-Sanguinet M,Moulia C,Slatko BE,Gavotte L

    更新日期:2020-06-30 00:00:00

  • Kinin release from human kininogen by 10 aspartic proteases produced by pathogenic yeast Candida albicans.

    abstract:BACKGROUND:Candida albicans yeast produces 10 distinct secreted aspartic proteases (Saps), which are some of the most important virulence factors of this pathogenic fungus. One of the suggested roles of Saps is their deregulating effect on various proteolytic cascades that constitute the major homeostatic systems in hu...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0394-8

    authors: Kozik A,Gogol M,Bochenska O,Karkowska-Kuleta J,Wolak N,Kamysz W,Aoki W,Ueda M,Faussner A,Rapala-Kozik M

    更新日期:2015-03-04 00:00:00

  • CoBaltDB: Complete bacterial and archaeal orfeomes subcellular localization database and associated resources.

    abstract:BACKGROUND:The functions of proteins are strongly related to their localization in cell compartments (for example the cytoplasm or membranes) but the experimental determination of the sub-cellular localization of proteomes is laborious and expensive. A fast and low-cost alternative approach is in silico prediction, bas...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-88

    authors: Goudenège D,Avner S,Lucchetti-Miganeh C,Barloy-Hubler F

    更新日期:2010-03-23 00:00:00

  • Antimicrobial activity of copper surfaces against suspensions of Salmonella enterica and Campylobacter jejuni.

    abstract:BACKGROUND:Salmonella enterica and Campylobacter jejuni are amongst the more prevalent bacterial pathogens that cause foodborne diseases. These microorganisms are common contaminants of poultry and poultry products. This study was aimed to evaluate the antibacterial activity of metallic copper surfaces on these importa...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-4-19

    authors: Faúndez G,Troncoso M,Navarrete P,Figueroa G

    更新日期:2004-04-30 00:00:00

  • Duodenal and faecal microbiota of celiac children: molecular, phenotype and metabolome characterization.

    abstract:BACKGROUND:Epidemiology of celiac disease (CD) is increasing. CD mainly presents in early childhood with small intestinal villous atrophy and signs of malabsorption. Compared to healthy individuals, CD patients seemed to be characterized by higher numbers of Gram-negative bacteria and lower numbers Gram-positive bacter...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-11-219

    authors: Di Cagno R,De Angelis M,De Pasquale I,Ndagijimana M,Vernocchi P,Ricciuti P,Gagliardi F,Laghi L,Crecchio C,Guerzoni ME,Gobbetti M,Francavilla R

    更新日期:2011-10-04 00:00:00

  • Biological and transcriptomic studies reveal hfq is required for swimming, biofilm formation and stress response in Xanthomonas axonpodis pv. citri.

    abstract:BACKGROUND:Hfq is a widely conserved bacterial RNA-binding protein which generally mediates the global regulatory activities involv ed in physiological process and virulence. The goal of this study was to characterize the biological function of hfq gene in Xanthomonas axonpodis pv. citri (Xac), the causal agent of citr...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1476-9

    authors: Liu X,Yan Y,Wu H,Zhou C,Wang X

    更新日期:2019-05-22 00:00:00