Abstract：
BACKGROUND:The Bacillus anthracis S-layer protein, BslA, plays a crucial role in mammalian infection. BslA is required to mediate adherence between host cells and vegetative forms of bacteria and this interaction promotes target organs adherence and blood-brain barrier (BBB) penetration in vivo. This study attempts to identify the potential eukaryotic ligand(s) for B. anthracis BslA protein. RESULTS:Biochemical approaches have indicated that the putative host cell ligand(s) for BslA is a surface protein, which is independent of the sugar components for binding to Bs1A. A ligand screening using blot overlays, far Western blots and mass spectrometry analyses revealed that BslA binds to mammalian laminin. ELISA based solid-phase binding assays and surface plasmon resonance assays demonstrated that there were high affinity interactions between BslA(260-652) and laminin. The SPR results also revealed the dissociation constants values of 3.172 × 10(-9)M for the binding of BslA(260-652) to laminin. CONCLUSIONS:These data demonstrated that laminin is a ligand for BslA.
journal_name
BMC Microbioljournal_title
BMC microbiologyauthors
Wang Y,Wei Y,Yuan S,Tao H,Dong J,Zhang Z,Tian W,Liu Cdoi
10.1186/s12866-016-0802-8subject
Has Abstractpub_date
2016-08-11 00:00:00pages
183issue
1issn
1471-2180pii
10.1186/s12866-016-0802-8journal_volume
16pub_type
杂志文章相关文献
BMC MICROBIOLOGY文献大全abstract：BACKGROUND:The phenomenon of misdiagnosing tuberculosis (TB) by laboratory cross-contamination when culturing Mycobacterium tuberculosis (MTB) has been widely reported and it has an obvious clinical, therapeutic and social impact. The final confirmation of a cross-contamination event requires the molecular identificati...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-8-30
更新日期：2008-02-14 00:00:00
abstract：BACKGROUND:Abundant populations of bacteria have been observed on Mir and the International Space Station. While some experiments have shown that bacteria cultured during spaceflight exhibit a range of potentially troublesome characteristics, including increases in growth, antibiotic resistance and virulence, other stu...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-13-241
更新日期：2013-11-06 00:00:00
abstract：BACKGROUND:Demand for high-throughput bioprocessing has dramatically increased especially in the biopharmaceutical industry because the technologies are of vital importance to process optimization and media development. This can be efficiently boosted by using microtiter plate (MTP) cultivation setup embedded into an a...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-017-1145-9
更新日期：2018-01-04 00:00:00
abstract：BACKGROUND:The genus Xanthomonas comprises several plant pathogenic bacteria affecting a wide range of hosts. Despite the economic, industrial and biological importance of Xanthomonas, the classification and phylogenetic relationships within the genus are still under active debate. Some of the relationships between pat...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-12-43
更新日期：2012-03-23 00:00:00
abstract：BACKGROUND:The gut comprises an essential barrier that protects both invertebrate and vertebrate animals from invasion by microorganisms. Disruption of the balanced relationship between indigenous gut microbiota and their host can result in gut bacteria eliciting host responses similar to those caused by invasive patho...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-10-129
更新日期：2010-04-27 00:00:00
abstract：BACKGROUND:Helicobacter pylori forms biofilms on glass surfaces at the air-liquid interface in in vitro batch cultures; however, biofilms of H. pylori have not been well characterized. In the present study, we analyzed the ability of H. pylori strains to form biofilms and characterized the underlying mechanisms of H. p...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-9-197
更新日期：2009-09-15 00:00:00
abstract：BACKGROUND:Identification and characterization of non-typeable Haemophilus influenzae (NTHi) with reduced susceptibility to β-lactam antibiotics due to mutations in penicillin binding protein 3 (PBP3) is a clinical challenge. We analyzed a blood isolate, NTHi93-57485, that was categorized as aminopenicillin resistant b...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-018-1196-6
更新日期：2018-05-31 00:00:00
abstract：BACKGROUND:Propionibacterium acnes is a commensal of human skin but is also known to be involved in certain diseases, such as acne vulgaris and infections of orthopaedic implants. Treatment of these conditions is complicated by increased resistance to antibiotics and/or biofilm formation of P. acnes bacteria. P. acnes ...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-8-139
更新日期：2008-08-15 00:00:00
abstract：BACKGROUND:Similar to Gram-negative bacteria, the outer membrane (OM) of the pathogenic spirochete, Borrelia burgdorferi, contains integral OM-spanning proteins (OMPs), as well as membrane-anchored lipoproteins. Although the mechanism of OMP biogenesis is still not well-understood, recent studies have indicated that a ...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-12-60
更新日期：2012-04-20 00:00:00
abstract：BACKGROUND:In humans, the intestinal microbiota plays an important role in the maintenance of host health by providing energy, nutrients, and immunological protection. Applying current molecular methods is necessary to surmount the limitations of classical culturing techniques in order to obtain an accurate description...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-9-123
更新日期：2009-06-09 00:00:00
abstract：BACKGROUND:Bacillus mycoides Flügge, a Gram-positive, non-motile soil bacterium assigned to Bacillus cereus group, grows on agar as chains of cells linked end to end, forming radial filaments curving clock- or counter-clockwise (SIN or DX morphotypes). The molecular mechanism causing asymmetric curving is not known: ou...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-2-33
更新日期：2002-11-13 00:00:00
abstract：BACKGROUND:Epidemiology of celiac disease (CD) is increasing. CD mainly presents in early childhood with small intestinal villous atrophy and signs of malabsorption. Compared to healthy individuals, CD patients seemed to be characterized by higher numbers of Gram-negative bacteria and lower numbers Gram-positive bacter...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-11-219
更新日期：2011-10-04 00:00:00
abstract：BACKGROUND:Neisseria gonorrhoeae is an obligate human pathogen that causes significant worldwide morbidity. N. gonorrhoeae expresses lipooligosaccharide (LOS), a phase variable molecule that plays an important role during pathogenesis of the organism. Alteration in the structure of gonococcal LOS correlates with altere...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-7-7
更新日期：2007-01-26 00:00:00
abstract：BACKGROUND:Hepatitis C virus (HCV) core protein and nonstructural protein 4B (NS4B) are potentially oncogenic. Aberrant activation of the Wnt/β-catenin signaling pathway is closely associated with hepatocarcinogenesis. We investigated the effects of HCV type 1b core protein and NS4B on Wnt/β-catenin signaling in variou...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-017-1032-4
更新日期：2017-05-25 00:00:00
abstract：BACKGROUND:Persister cells comprise a phenotypic variant that shows extreme antibiotic tolerance resulting in treatment failures of bacterial infections. While this phenomenon has posed a great threat in public health, mechanisms underlying their formation in Staphylococcus aureus remain largely unknown. Increasing evi...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-017-1129-9
更新日期：2017-11-22 00:00:00
abstract：BACKGROUND:During June-July 2012, six imipenem-resistant Escherichia coli isolates were isolated from two patients hospitalized in a ward of one large tertiary-care hospital in Genoa, Italy. Genetic features associated with blaNDM-4 gene were investigated. RESULTS:The isolates exhibited the same PFGE profile and a mul...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-14-148
更新日期：2014-06-07 00:00:00
abstract：BACKGROUND:The complete genome sequences of 44 Bacillus cereus group isolates collected from diverse sources in Japan were analyzed to determine their genetic backgrounds and diversity levels in Japan. Multilocus sequence typing (MLST) and core-genome single-nucleotide polymorphism (SNP) typing data from whole-genome s...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-019-1678-1
更新日期：2019-12-16 00:00:00
abstract：BACKGROUND:Alpha-isopropylmalate synthase (alpha-IPMS) is the key enzyme that catalyzes the first committed step in the leucine biosynthetic pathway. The gene encoding alpha-IPMS in Mycobacterium tuberculosis, leuA, is polymorphic due to the insertion of 57-bp repeat units referred to as Variable Number of Tandem Repea...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-9-122
更新日期：2009-06-09 00:00:00
abstract：BACKGROUND:Several conventional methods, including fungal culture and periodic acid-Schiff (PAS) reagent staining, have been used to diagnose oral candidiasis. The aim of this study was to evaluate the efficacy of a novel method, fungal fluorescent staining, in relation to conventional protocols in the diagnosis of ora...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-019-1467-x
更新日期：2019-05-14 00:00:00
abstract：BACKGROUND:Coxiella burnetii is the causative agent of Q-fever, a widespread zoonosis. Due to its high environmental stability and infectivity it is regarded as a category B biological weapon agent. In domestic animals infection remains either asymptomatic or presents as infertility or abortion. Clinical presentation i...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-8-77
更新日期：2008-05-19 00:00:00
abstract：BACKGROUND:The environment is the likely source of many pathogenic mycobacterial species but detection of mycobacteria by bacteriological tools is generally difficult and time-consuming. Consequently, several molecular targets based on the sequences of housekeeping genes, non-functional RNA and structural ribosomal RNA...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-13-277
更新日期：2013-12-03 00:00:00
abstract：BACKGROUND:Recent studies have identified in Mycobacterium avium subsp. paratuberculosis (MAP), already known as a pathogen in ruminants, a potential zoonotic agent of some autoimmune diseases in humans. Therefore, considering the possible risk for public health, it is necessary a thorough understanding of MAP's gene e...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-12-87
更新日期：2012-05-30 00:00:00
abstract：BACKGROUND:The kinetoplast DNA (kDNA) of trypanosomatids consists of an unusual arrangement of circular molecules catenated into a single network. The diameter of the isolated kDNA network is similar to that of the entire cell. However, within the kinetoplast matrix, the kDNA is highly condensed. Studies in Crithidia f...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-9-120
更新日期：2009-06-04 00:00:00
abstract：BACKGROUND:Since bacteria embedded in biofilms are far more difficult to eradicate than planktonic infections, it would be useful to know whether certain Staphylococcus aureus lineages are especially involved in strong biofilm formation. For this reason, in vitro biofilm formation of 228 clinical S. aureus isolates of ...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-9-229
更新日期：2009-10-28 00:00:00
abstract：BACKGROUND:Fecal microbiota transplantation (FMT) has been recently approved by FDA for the treatment of refractory recurrent clostridial colitis (rCDI). Success of FTM in treatment of rCDI led to a number of studies investigating the effectiveness of its application in the other gastrointestinal diseases. However, in ...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-019-1689-y
更新日期：2019-12-30 00:00:00
abstract：BACKGROUND:Mosquitoes are intermediate hosts for numerous disease causing organisms. Vector control is one of the most investigated strategy for the suppression of mosquito-borne diseases. Anopheles stephensi is one of the vectors of malaria parasite Plasmodium vivax. The parasite undergoes major developmental and matu...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-9-96
更新日期：2009-05-19 00:00:00
abstract：BACKGROUND:Nitric oxide (NO) is produced as part of the host immune response to bacterial infections, including urinary tract infections. The enzyme flavohemoglobin, coded by the hmp gene, is involved in protecting bacterial cells from the toxic effects of NO and represents a potentially interesting target for developm...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-14-65
更新日期：2014-03-14 00:00:00
abstract：BACKGROUND:Many plants have been used in traditional medicine for their antibacterial, antifungal, antiprotozoal, antiviral, antidiarrhoeal, analgesic, antimalarial, antioxidant, anti-inflammatory and anticancer activities. In order to find novel antimicrobial and antiviral agents, the aim of the present study was the ...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-017-1068-5
更新日期：2017-07-14 00:00:00
abstract：BACKGROUND:Bacteria release a wide variety of small molecules including cell-to-cell signaling compounds. Gram-negative bacteria use a variety of self-produced autoinducers such as acylated homoserine lactones (acyl-HSLs) as signal compounds for quorum sensing (QS) within and between bacterial species. QS plays a signi...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-12-70
更新日期：2012-05-10 00:00:00
abstract：BACKGROUND:After E. coli O157, E. coli O26 is the second most prevalent enterohaemorrhagic E. coli (EHEC) serotype identified in cases of foodborne illness in Australia and throughout the world. E. coli O26 associated foodborne outbreaks have drawn attention to the survival capabilities of this organism in a range of e...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-017-0963-0
更新日期：2017-03-01 00:00:00
