Abstract:
BACKGROUND:In humans, the intestinal microbiota plays an important role in the maintenance of host health by providing energy, nutrients, and immunological protection. Applying current molecular methods is necessary to surmount the limitations of classical culturing techniques in order to obtain an accurate description of the microbiota composition. RESULTS:Here we report on the comparative assessment of human fecal microbiota from three age-groups: infants, adults and the elderly. We demonstrate that the human intestinal microbiota undergoes maturation from birth to adulthood and is further altered with ageing. The counts of major bacterial groups Clostridium leptum, Clostridium coccoides, Bacteroidetes, Bifidobacterium, Lactobacillus and Escherichia coli were assessed by quantitative PCR (qPCR). By comparing species diversity profiles, we observed age-related changes in the human fecal microbiota. The microbiota of infants was generally characterized by low levels of total bacteria. C. leptum and C. coccoides species were highly represented in the microbiota of infants, while elderly subjects exhibited high levels of E. coli and Bacteroidetes. We observed that the ratio of Firmicutes to Bacteroidetes evolves during different life stages. For infants, adults and elderly individuals we measured ratios of 0.4, 10.9 and 0.6, respectively. CONCLUSION:In this work we have confirmed that qPCR is a powerful technique in studying the diverse and complex fecal microbiota. Our work demonstrates that the fecal microbiota composition evolves throughout life, from early childhood to old age.
journal_name
BMC Microbioljournal_title
BMC microbiologyauthors
Mariat D,Firmesse O,Levenez F,Guimarăes V,Sokol H,Doré J,Corthier G,Furet JPdoi
10.1186/1471-2180-9-123subject
Has Abstractpub_date
2009-06-09 00:00:00pages
123issn
1471-2180pii
1471-2180-9-123journal_volume
9pub_type
杂志文章相关文献
BMC MICROBIOLOGY文献大全abstract:BACKGROUND:Microbiota from different niches within the canine oral cavity were profiled and compared. Supragingival plaque and stimulated saliva, were collected alongside samples from the buccal and tongue dorsum mucosa, from 14 Labrador retrievers at three timepoints within a 1 month timeframe. The V3-V4 region of the...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-1704-3
更新日期:2020-02-28 00:00:00
abstract:BACKGROUND:Access to potable water remains a major challenge particularly in resource-limited settings. Although the potential contaminants of water are varied, enteric pathogenic protozoa are known to cause waterborne diseases greatly. This study aimed at investigating the prevalence, characteristics and correlates of...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0890-5
更新日期:2016-11-08 00:00:00
abstract:BACKGROUND:Adhesion to surfaces facilitates many crucial functions of microbes in their natural habitats. Thus understanding the mechanism of microbial adhesion is of broad interest to the microbiology research community. RESULTS:We report a study by fluorescence imaging and atomic force microscopy on the growth in si...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-139
更新日期:2013-06-19 00:00:00
abstract:BACKGROUND:Bartonella henselae is a Gram-negative bacterium transmitted to humans by a scratch from cat in the presence of ectoparasites. Humans infected with B. henselae can result in various clinical diseases including local lymphadenopathy and more serious systemic disease such as persistent bacteremia and endocardi...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01777-9
更新日期:2020-04-10 00:00:00
abstract:BACKGROUND:Acetic acid has been used to clean and disinfect surfaces in the household for many decades. The antimicrobial efficacy of cleaning procedures can be considered particularly important for young, old, pregnant, immunocompromised people, but may also concern other groups, particularly with regards to the COVID...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01948-8
更新日期:2020-08-26 00:00:00
abstract:BACKGROUND:Microorganisms of different species interact in several ecological niches, even causing infection. During the infectious process, a biofilm of single or multispecies can develop. Aspergillus fumigatus and Staphyloccocus aureus are etiologic agents that can cause infectious keratitis. We analyzed in vitro sin...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0363-2
更新日期:2015-02-15 00:00:00
abstract:BACKGROUND:The complete genome sequences of 44 Bacillus cereus group isolates collected from diverse sources in Japan were analyzed to determine their genetic backgrounds and diversity levels in Japan. Multilocus sequence typing (MLST) and core-genome single-nucleotide polymorphism (SNP) typing data from whole-genome s...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1678-1
更新日期:2019-12-16 00:00:00
abstract:BACKGROUND:Most group A streptococcal (GAS) vaccine strategies have focused on the surface M protein, a major virulence factor of GAS. The amino-terminus of the M protein elicits antibodies, that are both opsonic and protective, but which are type specific. J14, a chimeric peptide that contains 14 amino acids from the ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-6-71
更新日期:2006-08-09 00:00:00
abstract:: ...
journal_title:BMC microbiology
pub_type:
doi:10.1186/s12866-018-1279-4
更新日期:2018-11-23 00:00:00
abstract:BACKGROUND:Hfq is a widely conserved bacterial RNA-binding protein which generally mediates the global regulatory activities involv ed in physiological process and virulence. The goal of this study was to characterize the biological function of hfq gene in Xanthomonas axonpodis pv. citri (Xac), the causal agent of citr...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1476-9
更新日期:2019-05-22 00:00:00
abstract:BACKGROUND:Streptococcus canis is an important opportunistic pathogen of dogs and cats that can also infect a wide range of additional mammals including cows where it can cause mastitis. It is also an emerging human pathogen. RESULTS:Here we provide characterization of the first genome sequence for this species, strai...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-293
更新日期:2012-12-18 00:00:00
abstract:BACKGROUND:Nitric oxide (NO) is produced as part of the host immune response to bacterial infections, including urinary tract infections. The enzyme flavohemoglobin, coded by the hmp gene, is involved in protecting bacterial cells from the toxic effects of NO and represents a potentially interesting target for developm...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-14-65
更新日期:2014-03-14 00:00:00
abstract:BACKGROUND:Streptococcus pneumoniae is a common respiratory pathogen and a major causative agent of respiratory infections, including otitis media (OM). Pneumococcal biofilms have been demonstrated on biopsies of the middle ear mucosa in children receiving tympanostomy tubes, supporting the hypothesis that chronic OM m...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-173
更新日期:2008-10-08 00:00:00
abstract:BACKGROUND:Enterococcus is ubiquitous in nature and is a commensal of both the bovine and human gastrointestinal (GI) tract. It is also associated with clinical infections in humans. Subtherapeutic administration of antibiotics to cattle selects for antibiotic resistant enterococci in the bovine GI tract. Antibiotic re...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-0962-1
更新日期:2017-03-08 00:00:00
abstract:BACKGROUND:Wogonin, a natural flavonoid-like chemical compound, exhibits anti-inflammatory, antitumor, antiviral, neuroprotective, and anxiolytic effects by modulating a variety of cellular signaling pathways including PI3K-Akt, p53, nuclear factor κB (NF-κB), mitogen-activated protein kinase (MAPK) pathways. In this s...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01916-2
更新日期:2020-07-28 00:00:00
abstract:BACKGROUND:Pseudomonas aeruginosa is the predominant pathogen associated with the decline of pulmonary function in cystic fibrosis (CF) patients. Both environment-to-host acquisition and patient-to-patient transmission have been described for P. aeruginosa infection. Epidemic clones and bacterial phenotypic adaptation ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0760-1
更新日期:2016-07-11 00:00:00
abstract:BACKGROUND:Hepatitis B virus (HBV), because of its error-prone viral polymerase, has a high mutation rate leading to widespread substitutions, deletions, and insertions in the HBV genome. Deletions may significantly change viral biological features complicating the progression of liver diseases. However, the clinical c...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-307
更新日期:2012-12-28 00:00:00
abstract:BACKGROUND:Photorhabdus are Gram-negative nematode-symbiotic and insect-pathogenic bacteria. The species Photorhabdus asymbiotica is able to infect humans as well as insects. We investigated the secreted proteome of a clinical isolate of P. asymbiotica at different temperatures in order to identify proteins relevant to...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-141
更新日期:2010-05-12 00:00:00
abstract:BACKGROUND:Enterohemorrhagic Escherichia coli (EHEC) colonizes the intestinal epithelium and causes attaching and effacing (A/E) lesions. Expression of virulence genes, particularly those from the locus of the enterocyte effacement (LEE) pathogenicity island is required for the formation of a type three secretion syste...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-231
更新日期:2012-10-11 00:00:00
abstract:BACKGROUND:The increasing availability of whole genome sequences allows the gene or protein content of different organisms to be compared, leading to burgeoning interest in the relatively new subfield of pan-genomics. However, while several studies have analyzed protein content relationships in specific groups of bacte...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-258
更新日期:2010-10-13 00:00:00
abstract:BACKGROUND:We performed a 12-month cohort study of the stability and resilience of the intestinal microbiota of healthy children in daycare in Denmark in relation to diarrheal events and exposure to known risk factors for gastrointestinal health such as travelling and antibiotic use. In addition, we analyzed how gut mi...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1367-5
更新日期:2018-12-22 00:00:00
abstract:BACKGROUND:Cathepsins are a group of endosomal proteases present in many cells including dendritic cells (DCs). The activity of cathepsins is regulated by their endogenous inhibitors - cystatins. Cathepsins are crucial to antigen processing during viral and bacterial infections, and as such are a prerequisite to antige...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1471-1
更新日期:2019-05-10 00:00:00
abstract:BACKGROUND:Pseudomonas fuscovaginae (Pfv) is an emerging plant pathogen of rice and also of other gramineae plants. It causes sheath brown rot disease in rice with symptoms that are characterized by brown lesions on the flag leaf sheath, grain discoloration and sterility. It was first isolated as a high altitude pathog...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-014-0274-7
更新日期:2014-11-14 00:00:00
abstract:BACKGROUND:Poorly understood but highly diverse microbial communities exist within anoxic and oxygen-depleted marine sediments. These communities often harbour single-celled eukaryotes that form symbiotic associations with different prokaryotes. During low tides in South-western British Columbia, Canada, vast areas of ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-145
更新日期:2010-05-19 00:00:00
abstract:BACKGROUND:Rhizobium tropici strain PRF 81 (= SEMIA 4080) has been used in commercial inoculants for application to common-bean crops in Brazil since 1998, due to its high efficiency in fixing nitrogen, competitiveness against indigenous rhizobial populations and capacity to adapt to stressful tropical conditions, repr...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-84
更新日期:2012-05-30 00:00:00
abstract:BACKGROUND:The Gram-negative phytopathogenic bacterium Xanthomonas campestris pv. campestris recruits the hrp/T3SS system to inject pathogenicity effector proteins into host cells and uses the rpf/DSF cell-cell signaling system to regulate the expression of virulence factors such as extracellular enzymes and polysaccha...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1233-5
更新日期:2018-09-03 00:00:00
abstract:BACKGROUND:The Rio Grande River is the natural boundary between U.S. and Mexico from El Paso, TX to Brownsville, TX. and is one of the major water resources of the area. Agriculture, farming, maquiladora industry, domestic activities, as well as differences in disposal regulations and enforcement increase the contamina...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-4-17
更新日期:2004-04-22 00:00:00
abstract:BACKGROUND:Methicillin-resistant Staphylococcus aureus (MRSA) is known as a leading cause of morbidity and mortality. Investigation of the MRSA's virulence and resistance mechanisms is a continuing concern toward controlling such burdens through using high throughput whole Genome Sequencing (WGS) and molecular diagnost...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1470-2
更新日期:2019-06-11 00:00:00
abstract:BACKGROUND:Staphylococcus aureus is a food-borne pathogen and the most common cause of infections in hospitalized patients. The increase in the resistance of this pathogen to antibacterials has made necessary the development of new anti-staphylococcal agents. In this context, bacteriophage lytic enzymes such as endolys...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-11-138
更新日期:2011-06-17 00:00:00
abstract:BACKGROUND:Macrolides and lincosamides are two leading types of antibiotics commonly used in therapies. The study examines the differences in resistance to these antibiotics and their molecular bases in S. epidermidis as well as in rarely isolated species of coagulase-negative staphylococci such as S. hominis, S. haemo...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1496-5
更新日期:2019-06-10 00:00:00