Abstract:
BACKGROUND:Many plants have been used in traditional medicine for their antibacterial, antifungal, antiprotozoal, antiviral, antidiarrhoeal, analgesic, antimalarial, antioxidant, anti-inflammatory and anticancer activities. In order to find novel antimicrobial and antiviral agents, the aim of the present study was the evaluation of the antibacterial and antibiofilm susceptibility of Asphodelus microcarpus leaves extract. Moreover, the antiviral activity and the phytochemical composition of the active extract were also determined. METHODS:Antimicrobial and antibiofilm activities of leaves ethanol extract of A. microcarpus were evaluated on 13 different microbial strains. We selected three different sets of microorganisms: (i) Gram-positive bacteria, (ii) Gram-negative bacteria and (iii) yeasts. The potential antiviral activity of A. microcarpus leaves ethanol extract was evaluated with a luciferase reporter gene assay in which the dsRNA-dependent RIG-I-mediated IFN-β activation was inducted or inhibited by the Ebola virus VP35 protein. HPLC-DAD-MS was used to identify phenolic profile of the active extract. RESULTS:A. microcarpus leaves extract showed a potent inhibitory activity on Gram-positive bacteria while only a reduced inhibition was observed on Gram-negative bacteria. No activity was detected against Yeasts. The extract also showed an interesting antibiofilm motif on various bacterial strains (E. coli, S. aureus, S. haemolyticus and B. clausii). Moreover, this extract significantly affected the Ebola virus VP35 inhibition of the viral RNA (vRNA) induced IFN response. CONCLUSIONS:The overall results provide supportive data on the use of A. microcarpus as antimicrobial agent and a potential source of anti-viral natural products. Data collected set the bases for further studies for the identification of single active components and the development of new pharmaceuticals.
journal_name
BMC Microbioljournal_title
BMC microbiologyauthors
Di Petrillo A,Fais A,Pintus F,Santos-Buelga C,González-Paramás AM,Piras V,Orrù G,Mameli A,Tramontano E,Frau Adoi
10.1186/s12866-017-1068-5subject
Has Abstractpub_date
2017-07-14 00:00:00pages
159issue
1issn
1471-2180pii
10.1186/s12866-017-1068-5journal_volume
17pub_type
杂志文章相关文献
BMC MICROBIOLOGY文献大全abstract:BACKGROUND:Biofilm formation by Candida albicans has shown to be highly variable and is directly associated with pathogenicity and poor clinical outcomes in patients at risk. The aim of this study was to test the hypotheses that the extracellular DNA release by C. albicans is strain dependent and is associated with bio...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-014-0303-6
更新日期:2014-12-05 00:00:00
abstract:BACKGROUND:Anthrax, the zoonotic disease caused by the gram-positive bacterium Bacillus anthracis, is nowadays rare in northern parts of Europe including Finland and Scandinavia. Only two minor outbreaks of anthrax in 1988 and in 2004 and one sporadic infection in 2008 have been detected in animals in Finland since the...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1250-4
更新日期:2018-09-03 00:00:00
abstract:BACKGROUND:Modifiable lifestyle factors (e.g. dietary intake and physical activity) are important contributors to weight gain during college. The purpose of this study was to evaluate whether associations exist between body mass index, physical activity, screen time, dietary consumption (fat, protein, carbohydrates, an...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1362-x
更新日期:2018-12-12 00:00:00
abstract:BACKGROUND:After E. coli O157, E. coli O26 is the second most prevalent enterohaemorrhagic E. coli (EHEC) serotype identified in cases of foodborne illness in Australia and throughout the world. E. coli O26 associated foodborne outbreaks have drawn attention to the survival capabilities of this organism in a range of e...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-0963-0
更新日期:2017-03-01 00:00:00
abstract:BACKGROUND:Susceptibility to Bordetella pertussis infection varies widely. These differences can partly be explained by genetic host factors. HcB-28 mice are more resistant to B. pertussis infection than C3H mice, which could partially be ascribed to the B. pertussis susceptibility locus-1 (Bps1) on chromosome 12. The ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-7-88
更新日期:2007-10-12 00:00:00
abstract:BACKGROUND:Quorum sensing is a communication system that regulates gene expression in response to population density and often regulates virulence determinants. Deletion of the luxR homologue vjbR highly attenuates intracellular survival of Brucella melitensis and has been interpreted to be an indication of a role for ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-167
更新日期:2010-06-08 00:00:00
abstract:BACKGROUND:The increasing availability of whole genome sequences allows the gene or protein content of different organisms to be compared, leading to burgeoning interest in the relatively new subfield of pan-genomics. However, while several studies have analyzed protein content relationships in specific groups of bacte...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-258
更新日期:2010-10-13 00:00:00
abstract:BACKGROUND:During the last two decades research on animal filarial parasites, especially Onchocerca ochengi, infecting cattle in savanna areas of Africa revealed that O. ochengi as an animal model has biological features that are similar to those of O. volvulus, the aetiological agent of human onchocerciasis. There is,...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-02064-3
更新日期:2021-01-06 00:00:00
abstract:BACKGROUND:Total community rDNA was used to determine the diversity of bacteria and archaea from water, wet sediment and microbial mats samples of hot springs in the Eastern lowlands of Eritrea. The temperatures of the springs range from 49.5 °C to 100 °C while pH levels varied from 6.97 to 7.54. Akwar and Maiwooi have...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-1113-4
更新日期:2017-09-22 00:00:00
abstract:BACKGROUND:Prevotella intermedia is a Gram-negative black-pigmenting oral anaerobe associated with periodontitis in humans, and has a haem requirement for growth, survival and virulence. It produces an iron porphyrin-containing pigment comprising monomeric iron (III) protoporphyrin IX (Fe(III)PPIX.OH; haematin). The ba...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0516-3
更新日期:2015-09-24 00:00:00
abstract:BACKGROUND:Emergence of drug resistant varieties of tuberculosis is posing a major threat to global tuberculosis eradication programmes. Although several approaches have been explored to counter resistance, there has been limited success due to a lack of understanding of how resistance emerges in bacteria upon drug tre...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-234
更新日期:2008-12-23 00:00:00
abstract:BACKGROUND:Streptococcus canis is an important opportunistic pathogen of dogs and cats that can also infect a wide range of additional mammals including cows where it can cause mastitis. It is also an emerging human pathogen. RESULTS:Here we provide characterization of the first genome sequence for this species, strai...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-293
更新日期:2012-12-18 00:00:00
abstract:BACKGROUND:Tetrahymena thermophila is one of the best characterized unicellular eukaryotes and its genome is sequenced in its entirety. However, the AT-richness of the genome and an unusual codon usage cause problems in cloning and expression of the ciliate DNA. To overcome these technical hiatuses we developed a Cre-d...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-7-12
更新日期:2007-03-01 00:00:00
abstract:BACKGROUND:Pneumococcus is exposed to a variety of temperature and oxygen levels in the upper respiratory tract and as it invades the lung, tissues, and blood. We sought to determine the effect of environmental variability on growth in vitro and to assess variability between strains. We evaluated the effect of temperat...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1671-8
更新日期:2019-12-26 00:00:00
abstract:BACKGROUND:Epidemiology of celiac disease (CD) is increasing. CD mainly presents in early childhood with small intestinal villous atrophy and signs of malabsorption. Compared to healthy individuals, CD patients seemed to be characterized by higher numbers of Gram-negative bacteria and lower numbers Gram-positive bacter...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-11-219
更新日期:2011-10-04 00:00:00
abstract:BACKGROUND:Vibrio parahaemolyticus is a food-borne pathogen causing inflammation of the gastrointestinal epithelium. Pathogenic strains of this bacterium possess two Type III Secretion Systems (TTSS) that deliver effector proteins into host cells. In order to better understand human host cell responses to V. parahaemol...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-329
更新日期:2010-12-30 00:00:00
abstract:BACKGROUND:The balanced synthesis of membrane phospholipids, fatty acids and cell wall constituents is a vital facet of bacterial physiology, but there is little known about the biochemical control points that coordinate these activities in Gram-positive bacteria. In Escherichia coli, the glycerol-phosphate acyltransfe...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-260
更新日期:2013-11-16 00:00:00
abstract:BACKGROUND:Since 1994, Brucella strains have been isolated from a wide range of marine mammals. They are currently recognized as two new Brucella species, B. pinnipedialis for the pinniped isolates and B. ceti for the cetacean isolates in agreement with host preference and specific phenotypic and molecular markers. In ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-145
更新日期:2009-07-20 00:00:00
abstract:BACKGROUND:Acinetobacter baumannii is a gram-negative bacterium which causes opportunistic infections in immunocompromised hosts. Genome plasticity has given rise to a wide range of strain variation with respect to antimicrobial resistance profiles and expression of virulence factors which lead to altered phenotypes as...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-02082-1
更新日期:2021-01-09 00:00:00
abstract:BACKGROUND:Vibrio anguillarum is an extracellular bacterial pathogen that is a causative agent of vibriosis in finfish and crustaceans with mortality rates ranging from 30% to 100%. Mutations in central metabolism (glycolysis and the TCA cycle) of intracellular pathogens often result in attenuated virulence due to depl...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-1124-1
更新日期:2017-11-14 00:00:00
abstract:BACKGROUND:The Gifsy-I phage integrates into the Salmonella Typhimurium chromosome via an integrase mediated, site-specific recombination mechanism. Excision of the Gifsy-I phage requires three proteins, the Gifsy-I integrase (Int), the Gifsy-I excisionase (Xis) protein, and host encoded Integration Host Factor (IHF). ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-199
更新日期:2008-11-17 00:00:00
abstract:BACKGROUND:Famous Chinese strong-flavored liquor (CSFL) is brewed by microbial consortia in a special fermentation pit (FT). However, the fermentation process was not fully understood owing to the complicate community structure and metabolism. In this study, the process-related dynamics of microbial communities and mai...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-1106-3
更新日期:2017-09-15 00:00:00
abstract:BACKGROUND:Brucellosis incidence in China is divided into three stages: high incidence (1950s-1960s), decline (1970s-1980s), and re-emergence (1990s-2010s). At the re-emergence stage, Brucellosis incidence grew exponentially and spread to all 32 provinces. We describe the magnitude and the etiological distribution chan...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1149-0
更新日期:2018-01-10 00:00:00
abstract:BACKGROUND:Most studies on the human microbiota have analyzed stool samples, although a large proportion of the absorption of nutrients takes place in upper gut tract. We collected samples from different locations along the entire gastrointestinal tract from six patients who had simultaneously undergone upper endoscopy...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1304-7
更新日期:2018-10-24 00:00:00
abstract:BACKGROUND:The natural product Emodin demonstrates a wide range of pharmacological properties including anticancer, anti-inflammatory, antiproliferation, vasorelaxant and anti-H. pylori activities. Although its H. pylori inhibition was discovered, no acting target information against Emodin has been revealed to date. ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-91
更新日期:2009-05-12 00:00:00
abstract:BACKGROUND:Fungi are the second most abundant type of human pathogens. Invasive fungal pathogens are leading causes of life-threatening infections in clinical settings. Toxicity to the host and drug-resistance are two major deleterious issues associated with existing antifungal agents. Increasing a host's tolerance and...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-224
更新日期:2013-10-07 00:00:00
abstract:BACKGROUND:Quinolone resistant Escherichia coli (QREC) have been found in samples from Norwegian broiler chicken, despite quinolones not being administered to poultry in Norway. Biofilm production may be one factor contributing to the observed persistence in the broiler production chain. In the present study, 158 QREC ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01730-w
更新日期:2020-03-04 00:00:00
abstract:BACKGROUND:Hepatitis B virus (HBV), because of its error-prone viral polymerase, has a high mutation rate leading to widespread substitutions, deletions, and insertions in the HBV genome. Deletions may significantly change viral biological features complicating the progression of liver diseases. However, the clinical c...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-307
更新日期:2012-12-28 00:00:00
abstract:BACKGROUND:Non-typeable Haemophilus influenzae biofilm formation is implicated in a number of chronic infections including otitis media, sinusitis and bronchitis. Biofilm structure includes cells and secreted extracellular matrix that is "slimy" and believed to contribute to the antibiotic resistant properties of biofi...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-6-65
更新日期:2006-07-19 00:00:00
abstract:BACKGROUND:Metabolically versatile soil bacteria Burkholderia cepacia complex (Bcc) have emerged as opportunistic pathogens, especially of cystic fibrosis (CF). Previously, we initiated the characterization of the phenylacetic acid (PA) degradation pathway in B. cenocepacia, a member of the Bcc, and demonstrated the ne...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-222
更新日期:2009-10-18 00:00:00