Abstract:
BACKGROUND:Vibrio parahaemolyticus is a food-borne pathogen causing inflammation of the gastrointestinal epithelium. Pathogenic strains of this bacterium possess two Type III Secretion Systems (TTSS) that deliver effector proteins into host cells. In order to better understand human host cell responses to V. parahaemolyticus, the modulation of Mitogen Activated Protein Kinase (MAPK) activation in epithelial cells by an O3:K6 clinical isolate, RIMD2210633, was investigated. The importance of MAPK activation for the ability of the bacterium to be cytotoxic and to induce secretion of Interleukin-8 (IL-8) was determined. RESULTS:V. parahaemolyticus deployed its TTSS1 to induce activation of the JNK, p38 and ERK MAPK in human epithelial cells. VP1680 was identified as the TTSS1 effector protein responsible for MAPK activation in Caco-2 cells and the activation of JNK and ERK by this protein was important in induction of host cell death. V. parahaemolyticus actively induced IL-8 secretion in a response mediated by TTSS1. A role for VP1680 and for the ERK signalling pathway in the stimulation of IL-8 production in epithelial cells by V. parahaemolyticus was established. Interestingly, TTSS2 inhibited IL-8 mRNA transcription at early stages of interaction between the bacterium and the cell. CONCLUSIONS:This study demonstrated that V. parahaemolyticus activates the three major MAPK signalling pathways in intestinal epithelial cells in a TTSS1-dependent manner that involves the TTSS1 effector VP1680. Furthermore VP1680 and JNK and ERK activation were needed for maximal cytotoxicity of the bacterium. It was shown that V. parahaemolyticus is a strong inducer of IL-8 secretion and that induction reflects a balance between the effects of TTSS1 and TTSS2. Increases in IL-8 secretion were mediated by TTSS1 and VP1680, and augmented by ERK activation. These results shed light on the mechanisms of bacterial pathogenesis mediated by TTSS and suggest significant roles for MAPK signalling during infection with V. parahaemolyticus.
journal_name
BMC Microbioljournal_title
BMC microbiologyauthors
Matlawska-Wasowska K,Finn R,Mustel A,O'Byrne CP,Baird AW,Coffey ET,Boyd Adoi
10.1186/1471-2180-10-329subject
Has Abstractpub_date
2010-12-30 00:00:00pages
329issn
1471-2180pii
1471-2180-10-329journal_volume
10pub_type
杂志文章相关文献
BMC MICROBIOLOGY文献大全abstract:BACKGROUND:The bacterial surface protein internalin (InlA) is a major virulence factor of the food-born pathogen Listeria monocytogenes. It plays a critical role in the bacteria crossing the host intestinal barrier by a species-specific interaction with the cell adhesion molecule E-cadherin. In mice, the interaction of...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-90
更新日期:2013-04-23 00:00:00
abstract:BACKGROUND:Epitope tagging is a powerful strategy to study the function of proteins. Although tools for C-terminal protein tagging in the ciliated protozoan Tetrahymena thermophila have been developed, N-terminal protein tagging in this organism is still technically demanding. RESULTS:In this study, we have establishe...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-191
更新日期:2010-07-13 00:00:00
abstract:BACKGROUND:Microbiota from different niches within the canine oral cavity were profiled and compared. Supragingival plaque and stimulated saliva, were collected alongside samples from the buccal and tongue dorsum mucosa, from 14 Labrador retrievers at three timepoints within a 1 month timeframe. The V3-V4 region of the...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-1704-3
更新日期:2020-02-28 00:00:00
abstract:BACKGROUND:The complete genome sequences of 44 Bacillus cereus group isolates collected from diverse sources in Japan were analyzed to determine their genetic backgrounds and diversity levels in Japan. Multilocus sequence typing (MLST) and core-genome single-nucleotide polymorphism (SNP) typing data from whole-genome s...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1678-1
更新日期:2019-12-16 00:00:00
abstract:BACKGROUND:The emergence and spread of multidrug resistant methicillin-resistant Staphylococcus aureus (MDR-MRSA) has serious health consequences in the presence of sub-MIC antibiotics. Therefore, this study was designed to evaluate β-lactamase activity, efflux activity, biofilm formation, and gene expression pattern i...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0791-7
更新日期:2016-07-29 00:00:00
abstract:BACKGROUND:Identification and characterization of non-typeable Haemophilus influenzae (NTHi) with reduced susceptibility to β-lactam antibiotics due to mutations in penicillin binding protein 3 (PBP3) is a clinical challenge. We analyzed a blood isolate, NTHi93-57485, that was categorized as aminopenicillin resistant b...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1196-6
更新日期:2018-05-31 00:00:00
abstract:BACKGROUND:Streptococcus canis is an important opportunistic pathogen of dogs and cats that can also infect a wide range of additional mammals including cows where it can cause mastitis. It is also an emerging human pathogen. RESULTS:Here we provide characterization of the first genome sequence for this species, strai...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-293
更新日期:2012-12-18 00:00:00
abstract:BACKGROUND:Biofilms are ubiquitous. For instance, the majority of medical infections are thought to involve biofilms. However even after decades of investigation, the in vivo efficacy of many antimicrobial strategies is still debated suggesting there is a need for better understanding of biofilm antimicrobial tolerance...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-185
更新日期:2010-07-07 00:00:00
abstract:BACKGROUND:A biofilm is a complex community of microorganisms that develop on surfaces in diverse environments. The thickness of the biofilm plays a crucial role in the physiology of the immobilized bacteria. The most cariogenic bacteria, mutans streptococci, are common inhabitants of a dental biofilm community. In thi...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-236
更新日期:2008-12-29 00:00:00
abstract:BACKGROUND:Bacillus spp. have prominent ability to suppress plant pathogens and corresponding diseases. Previous analyses of Bacillus spp. revealed numerous gene clusters involved in nonribosomal synthesis of cyclic lipopeptides with distinct antimicrobial action. The 4'-phosphopantetheinyl transferase (PPTase) encoded...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-1134-z
更新日期:2017-12-04 00:00:00
abstract:BACKGROUND:Listeria monocytogenes has been implicated in several food borne outbreaks as well as sporadic cases of disease. Increased understanding of the biology of this organism is important in the prevention of food borne listeriosis. The infectivity of Listeria monocytogenes ScottA, cultivated with and without oxyg...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-7-55
更新日期:2007-06-14 00:00:00
abstract:BACKGROUND:Brucellosis is an important zoonosis caused by the genus Brucella. In addition Brucella represents potential biological warfare agents due to the high contagious rates for humans and animals. Therefore, the strain typing epidemiological tool may be crucial for tracing back source of infection in outbreaks an...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-66
更新日期:2009-04-07 00:00:00
abstract:BACKGROUND:Wolbachia are the most widely spread endosymbiotic bacteria, present in a wide variety of insects and two families of nematodes. As of now, however, relatively little genomic data has been available. The Wolbachia symbiont can be parasitic, as described for many arthropod systems, an obligate mutualist, as i...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01863-y
更新日期:2020-06-30 00:00:00
abstract:BACKGROUND:Fungi constitute an important yet frequently neglected component of the human microbiota with a possible role in health and disease. Fungi and bacteria colonise the infant gastrointestinal tract in parallel, yet most infant microbiome studies have ignored fungi. Milk is a source of diverse and viable bacteri...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01829-0
更新日期:2020-06-05 00:00:00
abstract:BACKGROUND:Accurate identification of Aspergillus species is a very important subject. Mass spectral fingerprinting using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is generally employed for the rapid identification of fungal isolates. However, the results are based on s...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-1009-3
更新日期:2017-04-26 00:00:00
abstract:BACKGROUND:Mob family proteins are conserved between animals, plants and fungi and are essential for the activation of NDR kinases that control crucial cellular processes like cytokinesis, proliferation and morphology. RESULTS:We identified a hypomorphic allele of ChMOB2 in a random insertional mutant (vir-88) of the ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-0932-7
更新日期:2017-01-19 00:00:00
abstract:BACKGROUND:Fluconazole (FLC), a triazole antifungal drug, is widely used for the maintenance therapy of cryptococcal meningoencephalitis, the most common opportunistic infection in AIDS patients. In this study, we examined changes in the gene expression profile of the C. neoformans reference strain H99 (serotype A) fol...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-11-97
更新日期:2011-05-11 00:00:00
abstract:BACKGROUND:The genus Brucella contains highly infectious species that are classified as biological threat agents. The timely detection and identification of the microorganism involved is essential for an effective response not only to biological warfare attacks but also to natural outbreaks. Matrix-assisted laser desor...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-11-267
更新日期:2011-12-23 00:00:00
abstract:BACKGROUND:Increasing age, several co-morbidities, environmental contamination, antibiotic exposure and other intestinal perturbations appear to be the greatest risk factors for C. difficile infection (CDI). Therefore, elderly care home residents are considered particularly vulnerable to the infection. The main objecti...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0848-7
更新日期:2016-10-01 00:00:00
abstract:BACKGROUND:LiaRS mediates Bacillus subtilis response to cell envelope perturbations. A third protein, LiaF, has an inhibitory role over LiaRS in the absence of stimulus. Together, LiaF and LiaRS form a three-component system characterized by an unusual stoichiometry, a 4:1 ratio between LiaS and LiaR, the significance ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01796-6
更新日期:2020-04-29 00:00:00
abstract:BACKGROUND:Infection with Brucella melitensis (B. melitensis) is one of the most important causes of abortion in goats and sheep, and also causes severe systemic disease in exposed humans. In Ethiopia, based on seroepidemiological studies, brucellosis is known to be endemic. However, there is little information on the ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1474-y
更新日期:2019-05-24 00:00:00
abstract:BACKGROUND:Bacteria excrete costly toxins to defend their ecological niche. The evolution of such antagonistic interactions between individuals is expected to depend on both the social environment and the strength of resource competition. Antagonism is expected to be weak among highly similar genotypes because most ind...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-40
更新日期:2012-03-22 00:00:00
abstract:BACKGROUND:The opportunistic pathogen, Pseudomonas aeruginosa is well known for its environmental and metabolic versatility, yet many of the functions of its gene-products remain to be fully elucidated. This study's objective was to illuminate the potential functions of under-described gene-products during the medicall...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1441-7
更新日期:2019-04-01 00:00:00
abstract:BACKGROUND:Mycoplasma pneumoniae has previously been characterized as a micro-organism that is genetically highly stable. In spite of this genetic stability, homologous DNA recombination has been hypothesized to lie at the basis of antigenic variation of the major surface protein, P1, of M. pneumoniae. In order to iden...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-167
更新日期:2008-10-02 00:00:00
abstract:BACKGROUND:The Gifsy-I phage integrates into the Salmonella Typhimurium chromosome via an integrase mediated, site-specific recombination mechanism. Excision of the Gifsy-I phage requires three proteins, the Gifsy-I integrase (Int), the Gifsy-I excisionase (Xis) protein, and host encoded Integration Host Factor (IHF). ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-199
更新日期:2008-11-17 00:00:00
abstract:BACKGROUND:Probiotics are increasingly applied to prevent and treat a range of infectious, immune related and gastrointestinal diseases. Despite this, the mechanisms behind the putative effects of probiotics are poorly understood. One of the suggested modes of probiotic action is modulation of the endogenous gut microb...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-1090-7
更新日期:2017-08-17 00:00:00
abstract:BACKGROUND:Most studies on the human microbiota have analyzed stool samples, although a large proportion of the absorption of nutrients takes place in upper gut tract. We collected samples from different locations along the entire gastrointestinal tract from six patients who had simultaneously undergone upper endoscopy...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1304-7
更新日期:2018-10-24 00:00:00
abstract:BACKGROUND:The human gastrointestinal (GI) tract microbiota is characterised by an abundance of uncultured bacteria most often assigned in phyla Firmicutes and Bacteroidetes. Diversity of this microbiota, even though approached with culture independent techniques in several studies, still requires more elucidation. The...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-68
更新日期:2009-04-08 00:00:00
abstract:BACKGROUND:The mce4 operon is one of the four homologues of mammalian cell entry (mce) operons of Mycobacterium tuberculosis. The mce4A (Rv3499c) gene within this operon is homologous to mce1A (Rv0169), that has a role in host cell invasion by M. tuberculosis. Our earlier reports show that mce4 operon is expressed duri...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-200
更新日期:2008-11-19 00:00:00
abstract:BACKGROUND:The role of Aspergillus fumigatus mycotoxins in the colonization of the respiratory tract by conidia has not been studied extensively, even though patients at risk from invasive aspergillosis frequently exhibit respiratory epithelium damage. In a previous study, we found that filtrates of A. fumigatus cultur...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-7-5
更新日期:2007-01-23 00:00:00