Long-term impact of fecal transplantation in healthy volunteers.

Abstract:

BACKGROUND:Fecal microbiota transplantation (FMT) has been recently approved by FDA for the treatment of refractory recurrent clostridial colitis (rCDI). Success of FTM in treatment of rCDI led to a number of studies investigating the effectiveness of its application in the other gastrointestinal diseases. However, in the majority of studies the effects of FMT were evaluated on the patients with initially altered microbiota. The aim of our study was to estimate effects of FMT on the gut microbiota composition in healthy volunteers and to monitor its long-term outcomes. RESULTS:We have performed a combined analysis of three healthy volunteers before and after capsule FMT by evaluating their general condition, adverse clinical effects, changes of basic laboratory parameters, and several immune markers. Intestinal microbiota samples were evaluated by 16S rRNA gene and shotgun sequencing. The data analysis demonstrated profound shift towards the donor microbiota taxonomic composition in all volunteers. Following FMT, all the volunteers exhibited gut colonization with donor gut bacteria and persistence of this effect for almost ∼1 year of observation. Transient changes of immune parameters were consistent with suppression of T-cell cytotoxicity. FMT was well tolerated with mild gastrointestinal adverse events, however, one volunteer developed a systemic inflammatory response syndrome. CONCLUSIONS:The FMT leads to significant long-term changes of the gut microbiota in healthy volunteers with the shift towards donor microbiota composition and represents a relatively safe procedure to the recipients without long-term adverse events.

journal_name

BMC Microbiol

journal_title

BMC microbiology

authors

Goloshchapov OV,Olekhnovich EI,Sidorenko SV,Moiseev IS,Kucher MA,Fedorov DE,Pavlenko AV,Manolov AI,Gostev VV,Veselovsky VA,Klimina KM,Kostryukova ES,Bakin EA,Shvetcov AN,Gumbatova ED,Klementeva RV,Shcherbakov AA,Gorchak

doi

10.1186/s12866-019-1689-y

subject

Has Abstract

pub_date

2019-12-30 00:00:00

pages

312

issue

1

issn

1471-2180

pii

10.1186/s12866-019-1689-y

journal_volume

19

pub_type

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