BB0324 and BB0028 are constituents of the Borrelia burgdorferi β-barrel assembly machine (BAM) complex.

Abstract:

BACKGROUND:Similar to Gram-negative bacteria, the outer membrane (OM) of the pathogenic spirochete, Borrelia burgdorferi, contains integral OM-spanning proteins (OMPs), as well as membrane-anchored lipoproteins. Although the mechanism of OMP biogenesis is still not well-understood, recent studies have indicated that a heterooligomeric OM protein complex, known as BAM (β-barrel assembly machine) is required for proper assembly of OMPs into the bacterial OM. We previously identified and characterized the essential β-barrel OMP component of this complex in B. burgdorferi, which we determined to be a functional BamA ortholog. RESULTS:In the current study, we report on the identification of two additional protein components of the B. burgdorferi BAM complex, which were identified as putative lipoproteins encoded by ORFs BB0324 and BB0028. Biochemical assays with a BamA-depleted B. burgdorferi strain indicate that BB0324 and BB0028 do not readily interact with the BAM complex without the presence of BamA, suggesting that the individual B. burgdorferi BAM components may associate only when forming a functional BAM complex. Cellular localization assays indicate that BB0324 and BB0028 are OM-associated subsurface lipoproteins, and in silico analyses indicate that BB0324 is a putative BamD ortholog. CONCLUSIONS:The combined data suggest that the BAM complex of B. burgdorferi contains unique protein constituents which differ from those found in other proteobacterial BAM complexes. The novel findings now allow for the B. burgdorferi BAM complex to be further studied as a model system to better our understanding of spirochetal OM biogenesis in general.

journal_name

BMC Microbiol

journal_title

BMC microbiology

authors

Lenhart TR,Kenedy MR,Yang X,Pal U,Akins DR

doi

10.1186/1471-2180-12-60

subject

Has Abstract

pub_date

2012-04-20 00:00:00

pages

60

issn

1471-2180

pii

1471-2180-12-60

journal_volume

12

pub_type

杂志文章
  • Detection of Burkholderia pseudomallei O-antigen serotypes in near-neighbor species.

    abstract:BACKGROUND:Burkholderia pseudomallei is the etiological agent of melioidosis and a CDC category B select agent with no available effective vaccine. Previous immunizations in mice have utilized the lipopolysaccharide (LPS) as a potential vaccine target because it is known as one of the most important antigenic epitopes ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-12-250

    authors: Stone JK,Mayo M,Grasso SA,Ginther JL,Warrington SD,Allender CJ,Doyle A,Georgia S,Kaestli M,Broomall SM,Karavis MA,Insalaco JM,Hubbard KS,McNew LA,Gibbons HS,Currie BJ,Keim P,Tuanyok A

    更新日期:2012-11-05 00:00:00

  • Virulence regulator AphB enhances toxR transcription in Vibrio cholerae.

    abstract:BACKGROUND:Vibrio cholerae is the causative agent of cholera. Extensive studies reveal that complicated regulatory cascades regulate expression of virulence genes, the products of which are required for V. cholerae to colonize and cause disease. In this study, we investigated the expression of the key virulence regulat...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-3

    authors: Xu X,Stern AM,Liu Z,Kan B,Zhu J

    更新日期:2010-01-06 00:00:00

  • Using NanoSIMS coupled with microfluidics to visualize the early stages of coral infection by Vibrio coralliilyticus.

    abstract:BACKGROUND:Global warming has triggered an increase in the prevalence and severity of coral disease, yet little is known about coral/pathogen interactions in the early stages of infection. The point of entry of the pathogen and the route that they take once inside the polyp is currently unknown, as is the coral's capac...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1173-0

    authors: Gibbin E,Gavish A,Domart-Coulon I,Kramarsky-Winter E,Shapiro O,Meibom A,Vardi A

    更新日期:2018-04-20 00:00:00

  • Establishment of a Cre/loxP recombination system for N-terminal epitope tagging of genes in Tetrahymena.

    abstract:BACKGROUND:Epitope tagging is a powerful strategy to study the function of proteins. Although tools for C-terminal protein tagging in the ciliated protozoan Tetrahymena thermophila have been developed, N-terminal protein tagging in this organism is still technically demanding. RESULTS:In this study, we have establishe...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-191

    authors: Busch CJ,Vogt A,Mochizuki K

    更新日期:2010-07-13 00:00:00

  • Early detection of viable Francisella tularensis in environmental matrices by culture-based PCR.

    abstract:BACKGROUND:Francisella tularensis is a fastidious, Gram-negative coccobacillus and is the causative agent of tularemia. To assess viability yet overcome lengthy incubation periods, a culture-based PCR method was used to detect early growth of the lowest possible number of F. tularensis cells. This method utilized a pre...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01748-0

    authors: Buse HY,Morris BJ,Rice EW

    更新日期:2020-03-25 00:00:00

  • Characterization of lpaH2 gene corresponding to lipopeptide synthesis in Bacillus amyloliquefaciens HAB-2.

    abstract:BACKGROUND:Bacillus spp. have prominent ability to suppress plant pathogens and corresponding diseases. Previous analyses of Bacillus spp. revealed numerous gene clusters involved in nonribosomal synthesis of cyclic lipopeptides with distinct antimicrobial action. The 4'-phosphopantetheinyl transferase (PPTase) encoded...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-017-1134-z

    authors: Jin P,Wang H,Liu W,Miao W

    更新日期:2017-12-04 00:00:00

  • Characterization of flagellins isolated from a highly motile strain of Lactobacillus agilis.

    abstract:BACKGROUND:Most lactic acid bacteria are non-motile but some of them are flagellated and exhibit motility. So far, motile lactobacilli have rarely been studied, and characteristics of their flagellins are poorly understood. In this study, a highly motile strain of Lactobacillus agilis was recruited for transcriptional ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0667-x

    authors: Kajikawa A,Midorikawa E,Masuda K,Kondo K,Irisawa T,Igimi S,Okada S

    更新日期:2016-03-22 00:00:00

  • Outer membrane protein P4 is not required for virulence in the human challenge model of Haemophilus ducreyi infection.

    abstract:BACKGROUND:Bacterial lipoproteins often play important roles in pathogenesis and can stimulate protective immune responses. Such lipoproteins are viable vaccine candidates. Haemophilus ducreyi, which causes the sexually transmitted disease chancroid, expresses a number of lipoproteins during human infection. One such l...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-14-166

    authors: Janowicz DM,Zwickl BW,Fortney KR,Katz BP,Bauer ME

    更新日期:2014-06-24 00:00:00

  • Combined prime-boost vaccination against tick-borne encephalitis (TBE) using a recombinant vaccinia virus and a bacterial plasmid both expressing TBE virus non-structural NS1 protein.

    abstract:BACKGROUND:Heterologous prime-boost immunization protocols using different gene expression systems have proven to be successful tools in protecting against various diseases in experimental animal models. The main reason for using this approach is to exploit the ability of expression cassettes to prime or boost the immu...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-5-45

    authors: Aleshin SE,Timofeev AV,Khoretonenko MV,Zakharova LG,Pashvykina GV,Stephenson JR,Shneider AM,Altstein AD

    更新日期:2005-08-02 00:00:00

  • Breakdown of albumin and haemalbumin by the cysteine protease interpain A, an albuminase of Prevotella intermedia.

    abstract:BACKGROUND:Prevotella intermedia is a Gram-negative black-pigmenting oral anaerobe associated with periodontitis in humans, and has a haem requirement for growth, survival and virulence. It produces an iron porphyrin-containing pigment comprising monomeric iron (III) protoporphyrin IX (Fe(III)PPIX.OH; haematin). The ba...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0516-3

    authors: Byrne DP,Manandhar SP,Potempa J,Smalley JW

    更新日期:2015-09-24 00:00:00

  • Genomic analyses of two novel biofilm-degrading methicillin-resistant Staphylococcus aureus phages.

    abstract:BACKGROUND:Methicillin-resistant Staphylococcus aureus (MRSA) biofilm producers represent an important etiological agent of many chronic human infections. Antibiotics and host immune responses are largely ineffective against bacteria within biofilms. Alternative actions and novel antimicrobials should be considered. In...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1484-9

    authors: Dakheel KH,Rahim RA,Neela VK,Al-Obaidi JR,Hun TG,Isa MNM,Yusoff K

    更新日期:2019-05-28 00:00:00

  • Toxigenic potential and antimicrobial susceptibility of Bacillus cereus group bacteria isolated from Tunisian foodstuffs.

    abstract:BACKGROUND:Despite the importance of the B. cereus group as major foodborne pathogens that may cause diarrheal and/or emetic syndrome(s), no study in Tunisia has been conducted in order to characterize the pathogenic potential of the B. cereus group. The aim of this study was to assess the sanitary potential risks of 1...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1571-y

    authors: Gdoura-Ben Amor M,Jan S,Baron F,Grosset N,Culot A,Gdoura R,Gautier M,Techer C

    更新日期:2019-08-24 00:00:00

  • Expression and subcellular localization of kinetoplast-associated proteins in the different developmental stages of Trypanosoma cruzi.

    abstract:BACKGROUND:The kinetoplast DNA (kDNA) of trypanosomatids consists of an unusual arrangement of circular molecules catenated into a single network. The diameter of the isolated kDNA network is similar to that of the entire cell. However, within the kinetoplast matrix, the kDNA is highly condensed. Studies in Crithidia f...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-120

    authors: Cavalcanti DP,Shimada MK,Probst CM,Souto-Padrón TC,de Souza W,Goldenberg S,Fragoso SP,Motta MC

    更新日期:2009-06-04 00:00:00

  • Cow manure application effectively regulates the soil bacterial community in tea plantation.

    abstract:BACKGROUND:Cow manure is not only an agricultural waste, but also an organic fertilizer resource. The application of organic fertilizer is a feasible practice to mitigate the soil degradation caused by overuse of chemical fertilizers, which can affect the bacterial diversity and community composition in soils. However,...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01871-y

    authors: Zhang S,Sun L,Wang Y,Fan K,Xu Q,Li Y,Ma Q,Wang J,Ren W,Ding Z

    更新日期:2020-07-01 00:00:00

  • Diet and irradiation effects on the bacterial community composition and structure in the gut of domesticated teneral and mature Queensland fruit fly, Bactrocera tryoni (Diptera: Tephritidae).

    abstract:BACKGROUND:Mass-rearing, domestication and gamma irradiation of tephritid fruit flies used in sterile insect technique (SIT) programmes can negatively impact fly quality and performance. Symbiotic bacteria supplied as probiotics to mass-reared fruit flies may help to overcome some of these issues. However, the effects ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1649-6

    authors: Woruba DN,Morrow JL,Reynolds OL,Chapman TA,Collins DP,Riegler M

    更新日期:2019-12-24 00:00:00

  • Secular trends of blood isolates in patients from a rural area population hospitalized in a tertiary center in a small city in Greece.

    abstract:BACKGROUND:Most of the studies evaluating the secular trends of blood isolates come from tertiary hospitals in urban areas. We sought to study the trends of the antimicrobial resistance of blood isolates in patients from a rural population hospitalized in a tertiary hospital in a small city in Greece. METHODS:We retro...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-6-41

    authors: Falagas ME,Bakossi A,Pappas VD,Holevas PV,Bouras A,Stamata E

    更新日期:2006-05-02 00:00:00

  • Safety evaluation of the antimicrobial peptide bovicin HC5 orally administered to a murine model.

    abstract:BACKGROUND:Bovicin HC5 is an antimicrobial peptide that shows a broad spectrum of activity and potential for biotechnological and therapeutic applications. To gain insight about the safety of bovicin HC5 application, the histological and immunostimulatory effects of orally administrated bovicin HC5 to BALB/c mice were ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-69

    authors: Paiva AD,Fernandes KM,Dias RS,Rocha Ados S,Oliveira LL,Neves CA,Paula SO,Mantovani HC

    更新日期:2013-03-27 00:00:00

  • Liver and blood cytokine microenvironment in HCV patients is associated to liver fibrosis score: a proinflammatory cytokine ensemble orchestrated by TNF and tuned by IL-10.

    abstract:BACKGROUND:In this study, we have evaluated the immunological status of hepatitis C virus (HCV)-infected patients aiming at identifying putative biomarkers associated with distinct degrees of liver fibrosis. Peripheral blood and tissue T-cells as well as cytokine levels were quantified by flow cytometry. RESULTS:Data ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0610-6

    authors: de Souza-Cruz S,Victória MB,Tarragô AM,da Costa AG,Pimentel JP,Pires EF,Araújo Lde P,Coelho-dos-Reis JG,Gomes Mde S,Amaral LR,Teixeira-Carvalho A,Martins-Filho OA,Victória Fda S,Malheiro A

    更新日期:2016-01-07 00:00:00

  • Genomic characterization of plant cell wall degrading enzymes and in silico analysis of xylanases and polygalacturonases of Fusarium virguliforme.

    abstract:BACKGROUND:Plant cell wall degrading enzymes (PCWDEs) are a subset of carbohydrate-active enzymes (CAZy) produced by plant pathogens to degrade plant cell walls. To counteract PCWDEs, plants release PCWDEs inhibitor proteins (PIPs) to reduce their impact. Several transgenic plants expressing exogenous PIPs that interac...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0761-0

    authors: Chang HX,Yendrek CR,Caetano-Anolles G,Hartman GL

    更新日期:2016-07-12 00:00:00

  • Genetic analysis of the capsule polysaccharide (K antigen) and exopolysaccharide genes in pandemic Vibrio parahaemolyticus O3:K6.

    abstract:BACKGROUND:Pandemic Vibrio parahaemolyticus has undergone rapid changes in both K- and O-antigens, making detection of outbreaks more difficult. In order to understand these rapid changes, the genetic regions encoding these antigens must be examined. In Vibrio cholerae and Vibrio vulnificus, both O-antigen and capsular...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-274

    authors: Chen Y,Dai J,Morris JG Jr,Johnson JA

    更新日期:2010-11-02 00:00:00

  • Pyrosequencing analysis revealed complex endogenetic microorganism community from natural DongChong XiaCao and its microhabitat.

    abstract:BACKGROUND:Ophiocordyceps sinensis (DongChong XiaCao (DCXC) in Chinese), a fungal parasite of caterpillars, is a traditional Chinese medicine. Bioactive components isolated from natural DCXC possess a wide range of pharmacological actions. Many efforts have been directed towards isolating the fungi based on culture-dep...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0813-5

    authors: Xia F,Liu Y,Guo MY,Shen GR,Lin J,Zhou XW

    更新日期:2016-08-26 00:00:00

  • Did granny know best? Evaluating the antibacterial, antifungal and antiviral efficacy of acetic acid for home care procedures.

    abstract:BACKGROUND:Acetic acid has been used to clean and disinfect surfaces in the household for many decades. The antimicrobial efficacy of cleaning procedures can be considered particularly important for young, old, pregnant, immunocompromised people, but may also concern other groups, particularly with regards to the COVID...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01948-8

    authors: Zinn MK,Bockmühl D

    更新日期:2020-08-26 00:00:00

  • Insights into the impact of flhF inactivation on Campylobacter jejuni colonization of chick and mice gut.

    abstract:BACKGROUND:Campylobacter jejuni (C. jejuni) is a leading cause of foodborne gastroenteritis worldwide. This bacterium lacks many of the classical virulence factors, and flagellum-associated persistent colonization has been shown to be crucial for its pathogenesis. The flagellum plays a multifunctional role in C. jejuni...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1318-1

    authors: Ren F,Li X,Tang H,Jiang Q,Yun X,Fang L,Huang P,Tang Y,Li Q,Huang J,Jiao XA

    更新日期:2018-10-22 00:00:00

  • Hydrogen peroxide-producing pyruvate oxidase from Lactobacillus delbrueckii is catalytically activated by phosphotidylethanolamine.

    abstract:BACKGROUND:Pyruvate oxidase (Pox) is an important enzyme in bacterial metabolism for increasing ATP production and providing a fitness advantage via hydrogen peroxide production. However, few Pox enzymes have been characterized from bacterial species. The tetrameric non-hydrogen-peroxide producing Pox from E. coli is a...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01788-6

    authors: Cornacchione LP,Hu LT

    更新日期:2020-05-24 00:00:00

  • Phenotypic and genotypic characterisation of multiple antibiotic-resistant Staphylococcus aureus exposed to subinhibitory levels of oxacillin and levofloxacin.

    abstract:BACKGROUND:The emergence and spread of multidrug resistant methicillin-resistant Staphylococcus aureus (MDR-MRSA) has serious health consequences in the presence of sub-MIC antibiotics. Therefore, this study was designed to evaluate β-lactamase activity, efflux activity, biofilm formation, and gene expression pattern i...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0791-7

    authors: Jo A,Ahn J

    更新日期:2016-07-29 00:00:00

  • Genotypic and phenotypic relatedness of Pseudomonas aeruginosa isolates among the major cystic fibrosis patient cohort in Italy.

    abstract:BACKGROUND:Pseudomonas aeruginosa is the predominant pathogen associated with the decline of pulmonary function in cystic fibrosis (CF) patients. Both environment-to-host acquisition and patient-to-patient transmission have been described for P. aeruginosa infection. Epidemic clones and bacterial phenotypic adaptation ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0760-1

    authors: Cigana C,Melotti P,Baldan R,Pedretti E,Pintani E,Iansa P,De Fino I,Favari F,Bergamini G,Tridello G,Cirillo DM,Assael BM,Bragonzi A

    更新日期:2016-07-11 00:00:00

  • Staphylococcus aureus Biofilm and Planktonic cultures differentially impact gene expression, mapk phosphorylation, and cytokine production in human keratinocytes.

    abstract:BACKGROUND:Many chronic diseases, such as non-healing wounds are characterized by prolonged inflammation and respond poorly to conventional treatment. Bacterial biofilms are a major impediment to wound healing. Persistent infection of the skin allows the formation of complex bacterial communities termed biofilm. Bacter...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-11-143

    authors: Secor PR,James GA,Fleckman P,Olerud JE,McInnerney K,Stewart PS

    更新日期:2011-06-21 00:00:00

  • Escherichia coli phylogenetic group determination and its application in the identification of the major animal source of fecal contamination.

    abstract:BACKGROUND:Escherichia coli strains are commonly found in the gut microflora of warm-blooded animals. These strains can be assigned to one of the four main phylogenetic groups, A, B1, B2 and D, which can be divided into seven subgroups (A0, A1, B1, B22, B23, D1 and D2), according to the combination of the three genetic...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-161

    authors: Carlos C,Pires MM,Stoppe NC,Hachich EM,Sato MI,Gomes TA,Amaral LA,Ottoboni LM

    更新日期:2010-06-01 00:00:00

  • Deregulation of phytoene-β-carotene synthase results in derepression of astaxanthin synthesis at high glucose concentration in Phaffia rhodozyma astaxanthin-overproducing strain MK19.

    abstract:BACKGROUND:A major obstacle to industrial-scale astaxanthin production by the yeast Phaffia rhodozyma is the strong inhibitory effect of high glucose concentration on astaxanthin synthesis. We investigated, for the first time, the mechanism of the regulatory effect of high glucose (> 100 g/L) at the metabolite and tran...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1507-6

    authors: Miao L,Chi S,Wu M,Liu Z,Li Y

    更新日期:2019-06-15 00:00:00

  • Optimization of expression conditions for a novel NZ2114-derived antimicrobial peptide-MP1102 under the control of the GAP promoter in Pichia pastoris X-33.

    abstract:BACKGROUND:The infections caused by antibiotic multidrug-resistant bacteria seriously threaten human health. To prevent and cure the infections caused by multidrug-resistant bacteria, new antimicrobial agents are required. Antimicrobial peptides are ideal therapy candidates for antibiotic-resistant pathogens. However, ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0389-5

    authors: Mao R,Teng D,Wang X,Zhang Y,Jiao J,Cao X,Wang J

    更新日期:2015-03-03 00:00:00