Identification and characterization of intervening sequences within 23S rRNA genes from more than 200 Campylobacter isolates from seven species including atypical campylobacters.

Abstract:

BACKGROUND:Identification and characterization of intervening sequences (IVSs) within 23S rRNA genes from Campylobacter organisms including atypical campylobacters were carried out using two PCR primer pairs, designed to generate helix 25 and 45 regions. RESULTS:Only C. sputorum biovar sputorum LMG7975 and fecalis LMG8531, LMG8534 and LMG6728 of a total of 204 Campylobacter isolates (n = 56 C. jejuni; n = 11 C. coli; n = 33 C. fetus; n = 43 C. upsaliensis; n = 30 C. hyointestinalis; n = 4 C. sputorum biovar sputorum; n = 5 C. sputorum biovar fecalis; n = 5 C. sputorum biovar paraureolyticus; n = 10 C. concisus; n = 7 C. curvus) were shown to carry IVSs in helix 25 region. C. sputorum biovar fecalis LMG8531 and LMG8534, interestingly, carried two different kinds of the 23S rRNA genes with and without the IVS, respectively. Consequently, in a total of 265 isolates of 269, including 65 C. lari isolates examined previously, the absence of IVSs was identified in the helix 25 region. In the helix 45 region, all the C. hyointestinalis, C. sputorum and C. concisus isolates were shown not to carry any IVSs. However, the 30 of 56 C. jejuni isolates (54%), 5 of 11 C. coli (45%), 25 of 33 C. fetus (76%), 30 of 43 C. upsaliensis (70%) and 6 of 7 C. curvus (90%) were shown to carry IVSs. In C. jejuni and C. upsaliensis isolates, two different kinds of the 23S rRNA genes were also identified to occur with and without IVSs in the helix 45 region, respectively. CONCLUSIONS:Secondary structure models were also constructed with all the IVSs identified in the present study. In the purified RNA fractions from the isolates which carried the 16S or 23S rRNA genes with the IVSs, no 16S or 23S rRNA was evident, respectively.

journal_name

BMC Microbiol

journal_title

BMC microbiology

authors

Tazumi A,Kakinuma Y,Misawa N,Moore JE,Millar BC,Matsuda M

doi

10.1186/1471-2180-9-256

subject

Has Abstract

pub_date

2009-12-11 00:00:00

pages

256

issn

1471-2180

pii

1471-2180-9-256

journal_volume

9

pub_type

杂志文章
  • Application of hierarchical oligonucleotide primer extension (HOPE) to assess relative abundances of ammonia- and nitrite-oxidizing bacteria.

    abstract:BACKGROUND:Establishing an optimal proportion of nitrifying microbial populations, including ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB), complete nitrite oxidizers (comammox) and ammonia-oxidizing archaea (AOA), is important for ensuring the efficiency of nitrification in water treatment systems...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-017-0998-2

    authors: Scarascia G,Cheng H,Harb M,Hong PY

    更新日期:2017-04-04 00:00:00

  • Bacillus anthracis S-layer protein BslA binds to extracellular matrix by interacting with laminin.

    abstract:BACKGROUND:The Bacillus anthracis S-layer protein, BslA, plays a crucial role in mammalian infection. BslA is required to mediate adherence between host cells and vegetative forms of bacteria and this interaction promotes target organs adherence and blood-brain barrier (BBB) penetration in vivo. This study attempts to ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0802-8

    authors: Wang Y,Wei Y,Yuan S,Tao H,Dong J,Zhang Z,Tian W,Liu C

    更新日期:2016-08-11 00:00:00

  • Biphasic toxicodynamic features of some antimicrobial agents on microbial growth: a dynamic mathematical model and its implications on hormesis.

    abstract:BACKGROUND:In the present work, we describe a group of anomalous dose-response (DR) profiles and develop a dynamic model that is able to explain them. Responses were obtained from conventional assays of three antimicrobial agents (nisin, pediocin and phenol) against two microorganisms (Carnobacterium piscicola and Leuc...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-220

    authors: Murado MA,Vázquez JA

    更新日期:2010-08-19 00:00:00

  • Phylogenetic group- and species-specific oligonucleotide probes for single-cell detection of lactic acid bacteria in oral biofilms.

    abstract:BACKGROUND:The purpose of this study was to design and evaluate fluorescent in situ hybridization (FISH) probes for the single-cell detection and enumeration of lactic acid bacteria, in particular organisms belonging to the major phylogenetic groups and species of oral lactobacilli and to Abiotrophia/Granulicatella. R...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-11-14

    authors: Quevedo B,Giertsen E,Zijnge V,Lüthi-Schaller H,Guggenheim B,Thurnheer T,Gmür R

    更新日期:2011-01-19 00:00:00

  • Probiotic characteristics of Lactobacillus plantarum E680 and its effect on Hypercholesterolemic mice.

    abstract:BACKGROUND:Probiotics have been reported to reduce total cholesterol levels in vitro, but more evidence is needed to determine the clinical relevance of this activity. Chinese traditional fermented pickles are a good source of lactic acid bacteria. Therefore, pickle samples were collected for screening lactic acid bact...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01922-4

    authors: Zheng ZY,Cao FW,Wang WJ,Yu J,Chen C,Chen B,Liu JX,Firrman J,Renye J,Ren DX

    更新日期:2020-08-04 00:00:00

  • Analysis of stress- and host cell-induced expression of the Mycobacterium tuberculosis inorganic pyrophosphatase.

    abstract:BACKGROUND:The gene encoding the inorganic pyrophosphatase (PPase) of the intracellular pathogen Legionella pneumophila is induced during intracellular infection, but is constitutively expressed in Escherichia coli. The causative agent of tuberculosis, Mycobacterium tuberculosis, contains a well conserved copy of PPase...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-1-3

    authors: Triccas JA,Gicquel B

    更新日期:2001-01-01 00:00:00

  • Differences between predicted outer membrane proteins of genotype 1 and 2 Mannheimia haemolytica.

    abstract:BACKGROUND:Mannheimia haemolytica strains isolated from North American cattle have been classified into two genotypes (1 and 2). Although members of both genotypes have been isolated from the upper and lower respiratory tracts of cattle with or without bovine respiratory disease (BRD), genotype 2 strains are much more ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01932-2

    authors: Clawson ML,Schuller G,Dickey AM,Bono JL,Murray RW,Sweeney MT,Apley MD,DeDonder KD,Capik SF,Larson RL,Lubbers BV,White BJ,Blom J,Chitko-McKown CG,Brichta-Harhay DM,Smith TPL

    更新日期:2020-08-12 00:00:00

  • Bacterial diversity analysis of larvae and adult midgut microflora using culture-dependent and culture-independent methods in lab-reared and field-collected Anopheles stephensi-an Asian malarial vector.

    abstract:BACKGROUND:Mosquitoes are intermediate hosts for numerous disease causing organisms. Vector control is one of the most investigated strategy for the suppression of mosquito-borne diseases. Anopheles stephensi is one of the vectors of malaria parasite Plasmodium vivax. The parasite undergoes major developmental and matu...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-96

    authors: Rani A,Sharma A,Rajagopal R,Adak T,Bhatnagar RK

    更新日期:2009-05-19 00:00:00

  • Microbial iron management mechanisms in extremely acidic environments: comparative genomics evidence for diversity and versatility.

    abstract:BACKGROUND:Iron is an essential nutrient but can be toxic at high intracellular concentrations and organisms have evolved tightly regulated mechanisms for iron uptake and homeostasis. Information on iron management mechanisms is available for organisms living at circumneutral pH. However, very little is known about how...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-203

    authors: Osorio H,Martínez V,Nieto PA,Holmes DS,Quatrini R

    更新日期:2008-11-24 00:00:00

  • Diet, physical activity and screen time but not body mass index are associated with the gut microbiome of a diverse cohort of college students living in university housing: a cross-sectional study.

    abstract:BACKGROUND:Modifiable lifestyle factors (e.g. dietary intake and physical activity) are important contributors to weight gain during college. The purpose of this study was to evaluate whether associations exist between body mass index, physical activity, screen time, dietary consumption (fat, protein, carbohydrates, an...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1362-x

    authors: Whisner CM,Maldonado J,Dente B,Krajmalnik-Brown R,Bruening M

    更新日期:2018-12-12 00:00:00

  • Commensal Streptococcus agalactiae isolated from patients seen at University Hospital of Londrina, Paraná, Brazil: capsular types, genotyping, antimicrobial susceptibility and virulence determinants.

    abstract:BACKGROUND:Streptococcus agalactiae or Group B Streptococci (GBS) have the ability to access various host sites, which reflects its adaptability to different environments during the course of infection. This adaptation is due to the expression of virulence factors that are involved with survival, invasion and bacterial...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-297

    authors: Otaguiri ES,Morguette AE,Tavares ER,dos Santos PM,Morey AT,Cardoso JD,Perugini MR,Yamauchi LM,Yamada-Ogatta SF

    更新日期:2013-12-21 00:00:00

  • Antimicrobial resistance and molecular detection of extended spectrum β-lactamase producing Escherichia coli isolates from raw meat in Greater Accra region, Ghana.

    abstract:BACKGROUND:Typically, raw meat can be contaminated with antibiotic resistant pathogens at unhygienic slaughter and sale points. Consumption of meat contaminated with antibiotic resistant E. coli is associated with grave health care consequences. The aim of this study was to determine the microbial quality of raw meat, ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01935-z

    authors: Dsani E,Afari EA,Danso-Appiah A,Kenu E,Kaburi BB,Egyir B

    更新日期:2020-08-14 00:00:00

  • RpfC regulates the expression of the key regulator hrpX of the hrp/T3SS system in Xanthomonas campestris pv. campestris.

    abstract:BACKGROUND:The Gram-negative phytopathogenic bacterium Xanthomonas campestris pv. campestris recruits the hrp/T3SS system to inject pathogenicity effector proteins into host cells and uses the rpf/DSF cell-cell signaling system to regulate the expression of virulence factors such as extracellular enzymes and polysaccha...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1233-5

    authors: Jiang BL,Jiang GF,Liu W,Yang LC,Yang LY,Wang L,Hang XH,Tang JL

    更新日期:2018-09-03 00:00:00

  • In silico evolutionary analysis of Helicobacter pylori outer membrane phospholipase A (OMPLA).

    abstract:BACKGROUND:In the past decade, researchers have proposed that the pldA gene for outer membrane phospholipase A (OMPLA) is important for bacterial colonization of the human gastric ventricle. Several conserved Helicobacter pylori genes have distinct genotypes in different parts of the world, biogeographic patterns that ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-12-206

    authors: Vollan HS,Tannaes T,Yamaoka Y,Bukholm G

    更新日期:2012-09-13 00:00:00

  • In vivo bioluminescence imaging and histopathopathologic analysis reveal distinct roles for resident and recruited immune effector cells in defense against invasive aspergillosis.

    abstract:BACKGROUND:Invasive aspergillosis (IA) is a major cause of infectious morbidity and mortality in immune compromised patients. Studies on the pathogenesis of IA have been limited by the difficulty to monitor disease progression in real-time. For real-time monitoring of the infection, we recently engineered a bioluminesc...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-105

    authors: Ibrahim-Granet O,Jouvion G,Hohl TM,Droin-Bergère S,Philippart F,Kim OY,Adib-Conquy M,Schwendener R,Cavaillon JM,Brock M

    更新日期:2010-04-08 00:00:00

  • Genomic analysis of methicillin-resistant Staphylococcus aureus strain SO-1977 from Sudan.

    abstract:BACKGROUND:Methicillin-resistant Staphylococcus aureus (MRSA) is known as a leading cause of morbidity and mortality. Investigation of the MRSA's virulence and resistance mechanisms is a continuing concern toward controlling such burdens through using high throughput whole Genome Sequencing (WGS) and molecular diagnost...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1470-2

    authors: Ali MS,Isa NM,Abedelrhman FM,Alyas TB,Mohammed SE,Ahmed AE,Ahmed ZSA,Lau NS,Garbi MI,Amirul AA,Seed AO,Omer RA,Mohamed SB

    更新日期:2019-06-11 00:00:00

  • Correction to: Genetic characterization of Bacillus anthracis in Guizhou Province, Southwest of China.

    abstract:ERRATUM:Upon publication of the original article (1) it was highlighted by the authors that a grant awarded to support the research work of the study was missed in the acknowledgements. It should also be acknowledged that the grant titled "Genotyping and Molecular Epidemiological Characteristic of Bacillus anthracis in...

    journal_title:BMC microbiology

    pub_type: 杂志文章,已发布勘误

    doi:10.1186/s12866-018-1172-1

    authors: Li S,Ma Q,Chen H,Wang D,Liu Y,Wei X,You L,Yao G,Tian K,Tang G

    更新日期:2018-04-12 00:00:00

  • Genotyping of Chlamydophila psittaci using a new DNA microarray assay based on sequence analysis of ompA genes.

    abstract:BACKGROUND:The currently used genotyping system for the avian zoonotic pathogen Chlamydophila (C.) psittaci has evolved from serology and is based on ompA sequence variations. It includes seven avian and two non-avian genotypes. Restriction enzyme cleavage of the amplified ompA gene and, less frequently, ompA sequencin...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-63

    authors: Sachse K,Laroucau K,Hotzel H,Schubert E,Ehricht R,Slickers P

    更新日期:2008-04-17 00:00:00

  • Characterization and regulation of the resistance-nodulation-cell division-type multidrug efflux pumps MdtABC and MdtUVW from the fire blight pathogen Erwinia amylovora.

    abstract:BACKGROUND:The Gram-negative bacterium Erwinia amylovora is the causal agent of the devastating disease fire blight in rosaceous plants such as apple, pear, quince, raspberry, and cotoneaster. In order to survive and multiply in a host, microbes must be able to circumvent the toxic effects of antimicrobial plant compou...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-14-185

    authors: Pletzer D,Weingart H

    更新日期:2014-07-11 00:00:00

  • Quantitative differential proteomics of yeast extracellular matrix: there is more to it than meets the eye.

    abstract:BACKGROUND:Saccharomyces cerevisiae multicellular communities are sustained by a scaffolding extracellular matrix, which provides spatial organization, and nutrient and water availability, and ensures group survival. According to this tissue-like biology, the yeast extracellular matrix (yECM) is analogous to the higher...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0550-1

    authors: Faria-Oliveira F,Carvalho J,Ferreira C,Hernáez ML,Gil C,Lucas C

    更新日期:2015-11-25 00:00:00

  • Evolutionary relationships of ATP-Binding Cassette (ABC) uptake porters.

    abstract:BACKGROUND:The ATP-Binding Cassette (ABC) functional superfamily includes integral transmembrane exporters that have evolved three times independently, forming three families termed ABC1, ABC2 and ABC3, upon which monophyletic ATPases have been superimposed for energy-coupling purposes [e.g., J Membr Biol 231(1):1-10, ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-98

    authors: Zheng WH,Västermark Å,Shlykov MA,Reddy V,Sun EI,Saier MH Jr

    更新日期:2013-05-06 00:00:00

  • Biofilm forming properties of quinolone resistant Escherichia coli from the broiler production chain and their dynamics in mixed biofilms.

    abstract:BACKGROUND:Quinolone resistant Escherichia coli (QREC) have been found in samples from Norwegian broiler chicken, despite quinolones not being administered to poultry in Norway. Biofilm production may be one factor contributing to the observed persistence in the broiler production chain. In the present study, 158 QREC ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01730-w

    authors: Nesse LL,Osland AM,Mo SS,Sekse C,Slettemeås JS,Bruvoll AEE,Urdahl AM,Vestby LK

    更新日期:2020-03-04 00:00:00

  • Comparative diversity of microbiomes and Resistomes in beef feedlots, downstream environments and urban sewage influent.

    abstract:BACKGROUND:Comparative knowledge of microbiomes and resistomes across environmental interfaces between animal production systems and urban settings is lacking. In this study, we executed a comparative analysis of the microbiota and resistomes of metagenomes from cattle feces, catch basin water, manured agricultural soi...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1548-x

    authors: Zaheer R,Lakin SM,Polo RO,Cook SR,Larney FJ,Morley PS,Booker CW,Hannon SJ,Van Domselaar G,Read RR,McAllister TA

    更新日期:2019-08-27 00:00:00

  • Acquisition of the lac operon by Salmonella enterica.

    abstract:BACKGROUND:Classical bacteriological characteristics of Salmonella enterica indicate that the members of this species are unable to utilize lactose as a carbon source. However, lactose-fermenting (Lac+) strains of several Salmonella serovars have been isolated from different foodborne outbreaks as well as different geo...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0511-8

    authors: Leonard SR,Lacher DW,Lampel KA

    更新日期:2015-08-25 00:00:00

  • Isolation, characterization, and assessment of lactic acid bacteria toward their selection as poultry probiotics.

    abstract:BACKGROUND:Probiotics are live microorganisms that, when administered in adequate amounts, confer a health benefit on the host, are now accepted as suitable alternatives to antibiotics in the control of animal infections and improving animal production. Lactic acid bacteria (LAB) with remarkable functional properties h...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1626-0

    authors: Reuben RC,Roy PC,Sarkar SL,Alam RU,Jahid IK

    更新日期:2019-11-12 00:00:00

  • Synergistic effects of baicalein with cefotaxime against Klebsiella pneumoniae through inhibiting CTX-M-1 gene expression.

    abstract:BACKGROUND:Generation of extended- spectrum β- lactamases is one of the major mechanisms by which clinical Klebsiella pneumoniae develop resistance to antibiotics. Combined antibiotics prove to be a relatively effective method of controlling such resistant strains. Some of Chinese herbal active ingredients are known to...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0797-1

    authors: Cai W,Fu Y,Zhang W,Chen X,Zhao J,Song W,Li Y,Huang Y,Wu Z,Sun R,Dong C,Zhang F

    更新日期:2016-08-08 00:00:00

  • Comparisons of resistance of CF and non-CF pathogens to hydrogen peroxide and hypochlorous acid oxidants in vitro.

    abstract:BACKGROUND:Cystic fibrosis (CF) lung disease has a unique profile of pathogens predominated by Pseudomonas aeruginosa (PsA) and Staphylococcus aureus (SA). These microorganisms must overcome host immune defense to colonize the CF lungs. Polymorphonuclear neutrophils are a major component of the host defense against bac...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-11-112

    authors: Bonvillain RW,Painter RG,Ledet EM,Wang G

    更新日期:2011-05-20 00:00:00

  • Genetic stability of Brucella abortus isolates from an outbreak by multiple-locus variable-number tandem repeat analysis (MLVA16).

    abstract:BACKGROUND:Brucellosis caused by Brucella abortus is one of the most important zoonoses in the world. Multiple-locus variable-number tandem repeat analysis (MLVA16) has been shown be a useful tool to epidemiological traceback studies in B. abortus infection. Thus, the present study aimed (i) to evaluate the genetic div...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-14-186

    authors: Dorneles EM,Santana JA,Alves TM,Pauletti RB,Mol JP,Heinemann MB,Lage AP

    更新日期:2014-07-11 00:00:00

  • A novel gene expression system for Ralstonia eutropha based on the T7 promoter.

    abstract:BACKGROUND:Ralstonia eutropha (syn. Cupriavidus necator) is a model microorganism for studying metabolism of polyhydroxyalkanoates (PHAs) and a potential chassis for protein expression due to various advantages. Although current plasmid systems of R. eutropha provide a basic platform for gene expression, the performanc...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01812-9

    authors: Hu M,Xiong B,Li Z,Liu L,Li S,Zhang C,Zhang X,Bi C

    更新日期:2020-05-19 00:00:00

  • Adhesive polypeptides of Staphylococcus aureus identified using a novel secretion library technique in Escherichia coli.

    abstract:BACKGROUND:Bacterial adhesive proteins, called adhesins, are frequently the decisive factor in initiation of a bacterial infection. Characterization of such molecules is crucial for the understanding of bacterial pathogenesis, design of vaccines and development of antibacterial drugs. Because adhesins are frequently di...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-11-117

    authors: Kylväjä R,Kankainen M,Holm L,Westerlund-Wikström B

    更新日期:2011-05-27 00:00:00