Abstract:
BACKGROUND:Bifidobacteria is one of the major gut commensal groups found in infants. Their colonization is commonly associated with beneficial effects to the host through mechanisms like niche occupation and nutrient competition against pathogenic bacteria. Iron is an essential element necessary for most microorganisms, including bifidobacteria and efficient competition for this micronutrient is linked to proliferation and persistence. For this research we hypothesized that bifidobacteria in the gut of iron deficient infants can efficiently sequester iron. The aim of the present study was to isolate bifidobacteria in fecal samples of iron deficient Kenyan infants and to characterize siderophore production and iron internalization capacity. RESULTS:Fifty-six bifidobacterial strains were isolated by streaking twenty-eight stool samples from Kenyan infants, in enrichment media. To target strains with high iron sequestration mechanisms, a strong iron chelator 2,2-dipyridyl was supplemented to the agar media. Bifidobacterial isolates were first identified to species level by 16S rRNA sequencing, yielding B. bifidum (19 isolates), B. longum (15), B. breve (11), B. kashiwanohense (7), B. pseudolongum (3) and B. pseudocatenulatum (1). While most isolated bifidobacterial species are commonly encountered in the infantile gut, B. kashiwanohense was not frequently reported in infant feces. Thirty strains from culture collections and 56 isolates were characterized for their siderophore production, tested by the CAS assay. Siderophore activity ranged from 3 to 89% siderophore units, with 35 strains (41%) exhibiting high siderophore activity, and 31 (36%) and 20 (23%) showing intermediate or low activity. The amount of internalized iron of 60 bifidobacteria strains selected for their siderophore activity, was in a broad range from 8 to118 μM Fe. Four strains, B. pseudolongum PV8-2, B. kashiwanohense PV20-2, B. bifidum PV28-2a and B. longum PV5-1 isolated from infant stool samples were selected for both high siderophore activity and iron internalization. CONCLUSIONS:A broad diversity of bifidobacteria were isolated in infant stools using iron limited conditions, with some strains exhibiting high iron sequestration properties. The ability of bifidobacteria to efficiently utilize iron sequestration mechanism such as siderophore production and iron internalization may confer an ecological advantage and be the basis for enhanced competition against enteropathogens.
journal_name
BMC Microbioljournal_title
BMC microbiologyauthors
Vazquez-Gutierrez P,Lacroix C,Jaeggi T,Zeder C,Zimmerman MB,Chassard Cdoi
10.1186/s12866-014-0334-zsubject
Has Abstractpub_date
2015-01-16 00:00:00pages
3issn
1471-2180pii
s12866-014-0334-zjournal_volume
15pub_type
杂志文章相关文献
BMC MICROBIOLOGY文献大全abstract:BACKGROUND:Several plant-pathogenic bacteria are transmitted by insect vector species that often also act as hosts. In this interface, these bacteria encounter plant endophytic, insect endosymbiotic and other microbes. Here, we used high throughput sequencing to examine the bacterial communities of five different psyll...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01895-4
更新日期:2020-07-20 00:00:00
abstract:BACKGROUND:The emergence of antibiotic resistant pathogenic bacteria has reduced our ability to combat infectious diseases. At the same time the numbers of new antibiotics reaching the market have decreased. This situation has created an urgent need to discover novel antibiotic scaffolds. Recently, the application of p...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0696-5
更新日期:2016-05-10 00:00:00
abstract:BACKGROUND:Ustilago esculenta, a typical dimorphic fungus could infect Zizania latifolia and induce host stem swollen to form an edible vegetable called Jiaobai in China. The strains differentiation especially in the mating ability and pathogenicity is closely related to different phenotypes of Jiaobai formed in the fi...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1588-2
更新日期:2019-09-05 00:00:00
abstract:BACKGROUND:Nitric oxide (NO) is produced as part of the host immune response to bacterial infections, including urinary tract infections. The enzyme flavohemoglobin, coded by the hmp gene, is involved in protecting bacterial cells from the toxic effects of NO and represents a potentially interesting target for developm...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-14-65
更新日期:2014-03-14 00:00:00
abstract:BACKGROUND:Crohn's disease (CD) is a high morbidity chronic inflammatory disorder of unknown aetiology. Adherent-invasive Escherichia coli (AIEC) has been recently implicated in the origin and perpetuation of CD. Because bacterial biofilms in the gut mucosa are suspected to play a role in CD and biofilm formation is a ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-202
更新日期:2009-09-21 00:00:00
abstract:BACKGROUND:Increasing age, several co-morbidities, environmental contamination, antibiotic exposure and other intestinal perturbations appear to be the greatest risk factors for C. difficile infection (CDI). Therefore, elderly care home residents are considered particularly vulnerable to the infection. The main objecti...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0848-7
更新日期:2016-10-01 00:00:00
abstract::Following publication of the original article [1], the authors reported an error in Fig. 2c. ...
journal_title:BMC microbiology
pub_type: 已发布勘误
doi:10.1186/s12866-020-01791-x
更新日期:2020-04-30 00:00:00
abstract:BACKGROUND:The native microflora associated with mosquitoes have important roles in mosquito development and vector competence. Sequencing of bacterial V3 region from 16S rRNA genes across the developmental stages of Culex mosquitoes (early and late larval instars, pupae and adults) was used to test the hypothesis that...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0475-8
更新日期:2015-07-24 00:00:00
abstract:BACKGROUND:Whole-genome sequencing using high throughput technologies has revolutionized and speeded up the scientific investigation of bacterial genetics, biochemistry, and molecular biology. Lactic acid bacteria (LABs) have been extensively used in fermentation and more recently as probiotics in food products that pr...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01980-8
更新日期:2020-10-23 00:00:00
abstract:BACKGROUND:Pyruvate oxidase (Pox) is an important enzyme in bacterial metabolism for increasing ATP production and providing a fitness advantage via hydrogen peroxide production. However, few Pox enzymes have been characterized from bacterial species. The tetrameric non-hydrogen-peroxide producing Pox from E. coli is a...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01788-6
更新日期:2020-05-24 00:00:00
abstract:BACKGROUND:Suppression subtractive hybridization (SSH) strategy was used with extraintestinal pathogenic Escherichia coli (EXPEC) that cause avian colibacillosis (avian pathogenic E. coli or APEC) and human urinary tract infections (uropathogenic E. coli or UPEC) to determine if they possessed genes that were host and/...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-7-81
更新日期:2007-08-30 00:00:00
abstract:BACKGROUND:The mce4 operon is one of the four homologues of mammalian cell entry (mce) operons of Mycobacterium tuberculosis. The mce4A (Rv3499c) gene within this operon is homologous to mce1A (Rv0169), that has a role in host cell invasion by M. tuberculosis. Our earlier reports show that mce4 operon is expressed duri...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-200
更新日期:2008-11-19 00:00:00
abstract:BACKGROUND:Candida parapsilosis typically is a commensal of human skin. However, when host immune defense is compromised or the normal microflora balance is disrupted, C. parapsilosis transforms itself into an opportunistic pathogen. Candida-derived lipase has been identified as potential virulence factor. Even though ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-11-122
更新日期:2011-05-29 00:00:00
abstract:BACKGROUND:Ralstonia eutropha (syn. Cupriavidus necator) is a model microorganism for studying metabolism of polyhydroxyalkanoates (PHAs) and a potential chassis for protein expression due to various advantages. Although current plasmid systems of R. eutropha provide a basic platform for gene expression, the performanc...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01812-9
更新日期:2020-05-19 00:00:00
abstract:BACKGROUND:HSV-1 is a common pathogen that infects 50-90% of the human population worldwide. HSV-1 causes numerous infection-related diseases, some of which are severely life-threatening. There are antiviral medications with activity against HSV-1. However, with the emergence of drug-resistant mutant strains of HSV-1, ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1639-8
更新日期:2019-12-07 00:00:00
abstract:BACKGROUND:The ColRS two-component system has been shown to contribute to the membrane functionality and stress tolerance of Pseudomonas putida as well as to the virulence of Pseudomonas aeruginosa and plant pathogenic Xanthomonas species. However, the conditions activating the ColRS pathway and the signal(s) sensed by...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-14-162
更新日期:2014-06-20 00:00:00
abstract:BACKGROUND:The skin microbiome serves as a first line defense against pathogens in vertebrates. In amphibians, it has the potential to protect against the chytrid fungus Batrachochytrium dendrobatis (Bd), a likely agent of amphibian declines. Alteration of the microbiome associated with unfavorable environmental change...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01979-1
更新日期:2020-09-22 00:00:00
abstract:BACKGROUND:Plant cell wall degrading enzymes (PCWDEs) are a subset of carbohydrate-active enzymes (CAZy) produced by plant pathogens to degrade plant cell walls. To counteract PCWDEs, plants release PCWDEs inhibitor proteins (PIPs) to reduce their impact. Several transgenic plants expressing exogenous PIPs that interac...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0761-0
更新日期:2016-07-12 00:00:00
abstract:BACKGROUND:Although Aspergillus fumigatus is an important human fungal pathogen there are few expression systems available to study the contribution of specific genes to the growth and virulence of this opportunistic mould. Regulatable promoter systems based upon prokaryotic regulatory elements in the E. coli tetracycl...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-5-1
更新日期:2005-01-13 00:00:00
abstract:BACKGROUND:Fungi constitute an important yet frequently neglected component of the human microbiota with a possible role in health and disease. Fungi and bacteria colonise the infant gastrointestinal tract in parallel, yet most infant microbiome studies have ignored fungi. Milk is a source of diverse and viable bacteri...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01829-0
更新日期:2020-06-05 00:00:00
abstract:BACKGROUND:The functions of proteins are strongly related to their localization in cell compartments (for example the cytoplasm or membranes) but the experimental determination of the sub-cellular localization of proteomes is laborious and expensive. A fast and low-cost alternative approach is in silico prediction, bas...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-88
更新日期:2010-03-23 00:00:00
abstract:BACKGROUND:We have previously shown that supernatant from Candida albicans (CA) culture contains a Secretory Interleukin (IL)-12 Inhibitory Factor (CA-SIIF), which inhibits IL-12 production by human monocytes. However, the effect of CA-SIIF on secretion of other cytokines by monocytes is unknown, and detailed character...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-31
更新日期:2008-02-19 00:00:00
abstract:BACKGROUND:Ophiocordyceps sinensis (DongChong XiaCao (DCXC) in Chinese), a fungal parasite of caterpillars, is a traditional Chinese medicine. Bioactive components isolated from natural DCXC possess a wide range of pharmacological actions. Many efforts have been directed towards isolating the fungi based on culture-dep...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0813-5
更新日期:2016-08-26 00:00:00
abstract:BACKGROUND:The date palm root borer Oryctes agamemnon (Coleoptera: Scarabaeidae) is one of the major pests of palms. In Saudi Arabia, both larvae and adults of Oryctes are particularly troublesome, especially during the establishment of young date palm orchards. Endosymbiotic bacteria are known to have a key role in fo...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0422-8
更新日期:2015-04-22 00:00:00
abstract:BACKGROUND:It has recently been reported that intermittent fasting shapes the gut microbiota to benefit health, but this effect may be influenced to the exact fasting protocols. The purpose of this study was to assess the effects of different daily fasting hours on shaping the gut microbiota in mice. Healthy C57BL/6 J ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01754-2
更新日期:2020-03-24 00:00:00
abstract:BACKGROUND:Enterohemorrhagic Escherichia coli (EHEC) colonizes the intestinal epithelium and causes attaching and effacing (A/E) lesions. Expression of virulence genes, particularly those from the locus of the enterocyte effacement (LEE) pathogenicity island is required for the formation of a type three secretion syste...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-231
更新日期:2012-10-11 00:00:00
abstract:BACKGROUND:Nontuberculous mycobacteria (NTM) are ubiquitous organisms and the incidence of NTM infections has been increasing in recent years. Mycobacteroides abscessus (M. abscessus) is one of the most antimicrobial-resistant NTM; however, no reliable antibiotic regimen can be officially advocated. We evaluated the ef...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-02000-5
更新日期:2020-10-19 00:00:00
abstract:BACKGROUND:The genus Brucella contains highly infectious species that are classified as biological threat agents. The timely detection and identification of the microorganism involved is essential for an effective response not only to biological warfare attacks but also to natural outbreaks. Matrix-assisted laser desor...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-11-267
更新日期:2011-12-23 00:00:00
abstract:BACKGROUND:Multi-locus sequence typing (MLST) is a portable, broadly applicable method for classifying bacterial isolates at an intra-species level. This methodology provides clinical and scientific investigators with a standardized means of monitoring evolution within bacterial populations. MLST uses the DNA sequences...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-14-143
更新日期:2014-06-03 00:00:00
abstract:BACKGROUND:Metabolically versatile soil bacteria Burkholderia cepacia complex (Bcc) have emerged as opportunistic pathogens, especially of cystic fibrosis (CF). Previously, we initiated the characterization of the phenylacetic acid (PA) degradation pathway in B. cenocepacia, a member of the Bcc, and demonstrated the ne...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-222
更新日期:2009-10-18 00:00:00