Abstract：
BACKGROUND:Ralstonia eutropha (syn. Cupriavidus necator) is a model microorganism for studying metabolism of polyhydroxyalkanoates (PHAs) and a potential chassis for protein expression due to various advantages. Although current plasmid systems of R. eutropha provide a basic platform for gene expression, the performance of the expression-inducing systems is still limited. In addition, the sizes of the cloned genes are limited due to the large sizes of the plasmid backbones. RESULTS:In this study, an R. eutropha T7 expression system was established by integrating a T7 RNA polymerase gene driven by the PBAD promoter into the genome of R. eutropha, as well as adding a T7 promoter into a pBBR1-derived plasmid for gene expression. In addition, the essential DNA sequence necessary for pBBR1 plasmid replication was identified, and the redundant parts were deleted reducing the expression plasmid size to 3392 bp, which improved the electroporation efficiency about 4 times. As a result, the highest expression level of RFP was enhanced, and the L-arabinose concentration for expression induction was decreased 20 times. CONCLUSIONS:The R. eutropha T7 expression system provides an efficient platform for protein production and synthetic biology applications.
journal_name
BMC Microbioljournal_title
BMC microbiologyauthors
Hu M,Xiong B,Li Z,Liu L,Li S,Zhang C,Zhang X,Bi Cdoi
10.1186/s12866-020-01812-9subject
Has Abstractpub_date
2020-05-19 00:00:00pages
121issue
1issn
1471-2180pii
10.1186/s12866-020-01812-9journal_volume
20pub_type
杂志文章相关文献
BMC MICROBIOLOGY文献大全abstract：BACKGROUND:Pandemic Vibrio parahaemolyticus has undergone rapid changes in both K- and O-antigens, making detection of outbreaks more difficult. In order to understand these rapid changes, the genetic regions encoding these antigens must be examined. In Vibrio cholerae and Vibrio vulnificus, both O-antigen and capsular...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-10-274
更新日期：2010-11-02 00:00:00
abstract：BACKGROUND:The microbiological diagnosis of bacterial vaginosis is usually made using Nugent's criteria, a useful but rather laborious scoring system based on counting bacterial cell types on Gram stained slides of vaginal smears. Ison and Hay have simplified the score system to three categories and added a fourth cate...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-5-61
更新日期：2005-10-14 00:00:00
abstract：BACKGROUND:Global warming has triggered an increase in the prevalence and severity of coral disease, yet little is known about coral/pathogen interactions in the early stages of infection. The point of entry of the pathogen and the route that they take once inside the polyp is currently unknown, as is the coral's capac...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-018-1173-0
更新日期：2018-04-20 00:00:00
abstract：BACKGROUND:Previous phylogenetic analyses of species within the genus Golovinomyces (Ascomycota, Erysiphales), based on ITS and 28S rDNA sequence data, revealed a co-evolutionary relationship between powdery mildew species and hosts of certain tribes of the plant family Asteraceae. Golovinomyces growing on host plants ...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-020-01731-9
更新日期：2020-03-05 00:00:00
abstract：BACKGROUND:Most studies on the human microbiota have analyzed stool samples, although a large proportion of the absorption of nutrients takes place in upper gut tract. We collected samples from different locations along the entire gastrointestinal tract from six patients who had simultaneously undergone upper endoscopy...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-018-1304-7
更新日期：2018-10-24 00:00:00
abstract：BACKGROUND:The opportunistic pathogen, Pseudomonas aeruginosa is well known for its environmental and metabolic versatility, yet many of the functions of its gene-products remain to be fully elucidated. This study's objective was to illuminate the potential functions of under-described gene-products during the medicall...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-019-1441-7
更新日期：2019-04-01 00:00:00
abstract：BACKGROUND:Exposure of cells to environmental stress conditions can lead to the interruption of several intracellular processes, in particular those performed by macromolecular complexes such as the spliceosome. RESULTS:During nucleotide sequencing of cDNA libraries constructed using RNA isolated from B. emersonii cel...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-9-231
更新日期：2009-10-29 00:00:00
abstract：BACKGROUND:Mycoplasma pneumoniae has previously been characterized as a micro-organism that is genetically highly stable. In spite of this genetic stability, homologous DNA recombination has been hypothesized to lie at the basis of antigenic variation of the major surface protein, P1, of M. pneumoniae. In order to iden...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-8-167
更新日期：2008-10-02 00:00:00
abstract：BACKGROUND:The phenomenon of misdiagnosing tuberculosis (TB) by laboratory cross-contamination when culturing Mycobacterium tuberculosis (MTB) has been widely reported and it has an obvious clinical, therapeutic and social impact. The final confirmation of a cross-contamination event requires the molecular identificati...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-8-30
更新日期：2008-02-14 00:00:00
abstract：BACKGROUND:Compared with many protists, Giardia lamblia has a simple life cycle alternating between cyst and trophozoite. Most research on the molecular biology of Giardia parasites has focused on trophozoites and the processes of excystation and encystation, whereas cysts have attracted less interest. The striking mor...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-11-91
更新日期：2011-05-04 00:00:00
abstract：BACKGROUND:A growing body of evidence suggests that Mycobacterium tuberculosis (Mtb) uses the host's cholesterol as a source of carbon and energy during infection. Strains defective in cholesterol transport or degradation exhibit attenuated growth in activated macrophages and diminished infectivity in animal models. Th...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-13-43
更新日期：2013-02-20 00:00:00
abstract：BACKGROUND:Whole-genome sequencing using high throughput technologies has revolutionized and speeded up the scientific investigation of bacterial genetics, biochemistry, and molecular biology. Lactic acid bacteria (LABs) have been extensively used in fermentation and more recently as probiotics in food products that pr...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-020-01980-8
更新日期：2020-10-23 00:00:00
abstract：BACKGROUND:Chlortetracycline (CTC) is one of the commercially important tetracyclines (TCs) family product and is mainly produced by Streptomyces. CTC is still in a great demand due to its broad-spectrum activity against pathogens. Engineering transcriptional control allows the cell to allocate its valuable resources t...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-019-1670-9
更新日期：2019-12-10 00:00:00
abstract：BACKGROUND:Microencapsulated organic acids and botanicals have the potential to develop into important tools for the poultry industry. A blend of organic acids and botanicals (AviPlus®P) has previously shown to reduce Salmonella and Campylobacter in chickens; however, changes to the microbiota of the jejunum and ileum ...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-020-02001-4
更新日期：2020-11-02 00:00:00
abstract：BACKGROUND:The hemibiotrophic fungus Moniliophthora perniciosa is the causal agent of Witches' broom, a disease of Theobroma cacao. The pathogen life cycle ends with the production of basidiocarps in dead tissues of the infected host. This structure generates millions of basidiospores that reinfect young tissues of the...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-9-158
更新日期：2009-08-04 00:00:00
abstract：BACKGROUND:Non typhoidal salmonellosis is one of the neglected zoonoses in most African countries. The use of sub-therapeutic doses of antibiotics as animal growth promoter enhances the emergence and dissemination of antimicrobial resistance in bacteria with food animal reservoirs and may also results in antibiotics re...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-018-1248-y
更新日期：2018-08-30 00:00:00
abstract：BACKGROUND:Polymicrobial infections are responsible for significant mortality and morbidity in adults and children. Staphylococcus epidermidis and Candida albicans are the most frequent combination of organisms isolated from polymicrobial infections. Vascular indwelling catheters are sites for mixed species biofilm for...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-13-257
更新日期：2013-11-14 00:00:00
abstract：BACKGROUND:Fluconazole (FLC), a triazole antifungal drug, is widely used for the maintenance therapy of cryptococcal meningoencephalitis, the most common opportunistic infection in AIDS patients. In this study, we examined changes in the gene expression profile of the C. neoformans reference strain H99 (serotype A) fol...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-11-97
更新日期：2011-05-11 00:00:00
abstract：BACKGROUND:Infections caused by Shewanella spp. have been increasingly reported worldwide. The advances in genomic sciences have enabled better understanding about the taxonomy and epidemiology of this agent. However, the scarcity of DNA sequencing data is still an obstacle for understanding the genus and its associati...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-020-02040-x
更新日期：2020-11-24 00:00:00
abstract：BACKGROUND:Despite the importance of the B. cereus group as major foodborne pathogens that may cause diarrheal and/or emetic syndrome(s), no study in Tunisia has been conducted in order to characterize the pathogenic potential of the B. cereus group. The aim of this study was to assess the sanitary potential risks of 1...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-019-1571-y
更新日期：2019-08-24 00:00:00
abstract：BACKGROUND:GeoChip 3.0, a microbial functional gene array, containing ~28,000 oligonucleotide probes and targeting ~57,000 sequences from 292 functional gene families, provided a powerful tool for researching microbial community structure in natural environments. The alpine meadow is a dominant plant community in the Q...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-13-72
更新日期：2013-03-29 00:00:00
abstract：BACKGROUND:Papua New Guinea (PNG) is a high tuberculosis (TB) burden country of the WHO Western Pacific Region, but so far research on drug resistance (DR) and genotypes of Mycobacterium tuberculosis (M. tuberculosis) was only conducted in few provinces in the country. The aim of the present study was to obtain baselin...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-014-0307-2
更新日期：2014-12-05 00:00:00
abstract：BACKGROUND:The evolution of mycoplasmas from a common ancestor with Firmicutes has been characterized not only by genome down-sizing but also by horizontal gene transfer between mycoplasma species sharing a common host. The mechanisms of these gene transfers remain unclear because our knowledge of the mycoplasma mobile...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-12-257
更新日期：2012-11-12 00:00:00
abstract：BACKGROUND:Microbiome and metagenomic studies have given rise to a new understanding of microbial colonization of various human tissues and their ability to impact our health. One human microbiome growing in notoriety, the vaginal microbiome, stands out given its importance for women's health, and is peculiar in terms ...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-020-02027-8
更新日期：2020-11-10 00:00:00
abstract：BACKGROUND:Campylobacter jejuni (C. jejuni) is a leading cause of foodborne gastroenteritis worldwide. This bacterium lacks many of the classical virulence factors, and flagellum-associated persistent colonization has been shown to be crucial for its pathogenesis. The flagellum plays a multifunctional role in C. jejuni...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-018-1318-1
更新日期：2018-10-22 00:00:00
abstract：BACKGROUND:The type IV secretion system (T4SS) can be classified as a large family of macromolecule transporter systems, divided into three recognized sub-families, according to the well-known functions. The major sub-family is the conjugation system, which allows transfer of genetic material, such as a nucleoprotein, ...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-12-172
更新日期：2012-08-09 00:00:00
abstract：BACKGROUND:Brucellosis is an important zoonosis caused by the genus Brucella. In addition Brucella represents potential biological warfare agents due to the high contagious rates for humans and animals. Therefore, the strain typing epidemiological tool may be crucial for tracing back source of infection in outbreaks an...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-9-66
更新日期：2009-04-07 00:00:00
abstract：BACKGROUND:Microorganisms are a large and diverse form of life. Many of them live in association with large multicellular organisms, developing symbiotic relations with the host and some have even evolved to form obligate endosymbiosis. All Carpenter ants (genus Camponotus) studied hitherto harbour primary endosymbioti...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-9-29
更新日期：2009-02-06 00:00:00
abstract：BACKGROUND:The Rio Grande River is the natural boundary between U.S. and Mexico from El Paso, TX to Brownsville, TX. and is one of the major water resources of the area. Agriculture, farming, maquiladora industry, domestic activities, as well as differences in disposal regulations and enforcement increase the contamina...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/1471-2180-4-17
更新日期：2004-04-22 00:00:00
abstract：BACKGROUND:There is an urgent need to develop potent antimicrobials for the treatment of infections caused by antibiotic-resistant bacterial pathogens. Paenipeptin C' (C8-Pat) is a novel linear lipopeptide recently discovered by our group. The objectives of this study were to determine the time-kill kinetics of paenipe...
journal_title：BMC microbiology
pub_type： 杂志文章
doi：10.1186/s12866-018-1381-7
更新日期：2019-01-08 00:00:00
