Lab on a chip genotyping for Brucella spp. based on 15-loci multi locus VNTR analysis.

Abstract:

BACKGROUND:Brucellosis is an important zoonosis caused by the genus Brucella. In addition Brucella represents potential biological warfare agents due to the high contagious rates for humans and animals. Therefore, the strain typing epidemiological tool may be crucial for tracing back source of infection in outbreaks and discriminating naturally occurring outbreaks versus bioterroristic event. A Multiple Locus Variable-number tandem repeats (VNTR) Analysis (MLVA) assay based on 15 polymorphic markers was previously described. The obtained MLVA band profiles may be resolved by techniques ranging from low cost manual agarose gels to the more expensive capillary electrophoresis sequencing. In this paper a rapid, accurate and reproducible system, based on the Lab on a chip technology was set up for Brucella spp. genotyping. RESULTS:Seventeen DNA samples of Brucella strains isolated in Sicily, previously genotyped, and twelve DNA samples, provided by MLVA Brucella VNTR ring trial, were analyzed by MLVA-15 on Agilent 2100. The DNA fragment sizes produced by Agilent, compared with those expected, showed discrepancies; therefore, in order to assign the correct alleles to the Agilent DNA fragment sizes, a conversion table was produced. In order to validate the system twelve unknown DNA samples were analyzed by this method obtaining a full concordance with the VNTR ring trial results. CONCLUSION:In this paper we described a rapid and specific detection method for the characterization of Brucella isolates. The comparison of the MLVA typing data produced by Agilent system with the data obtained by standard sequencing or ethidium bromide slab gel electrophoresis showed a general concordance of the results. Therefore this platform represents a fair compromise among costs, speed and specificity compared to any conventional molecular typing technique.

journal_name

BMC Microbiol

journal_title

BMC microbiology

authors

De Santis R,Ciammaruconi A,Faggioni G,D'Amelio R,Marianelli C,Lista F

doi

10.1186/1471-2180-9-66

subject

Has Abstract

pub_date

2009-04-07 00:00:00

pages

66

issn

1471-2180

pii

1471-2180-9-66

journal_volume

9

pub_type

杂志文章
  • MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis.

    abstract:BACKGROUND:Since 1994, Brucella strains have been isolated from a wide range of marine mammals. They are currently recognized as two new Brucella species, B. pinnipedialis for the pinniped isolates and B. ceti for the cetacean isolates in agreement with host preference and specific phenotypic and molecular markers. In ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-145

    authors: Maquart M,Le Flèche P,Foster G,Tryland M,Ramisse F,Djønne B,Al Dahouk S,Jacques I,Neubauer H,Walravens K,Godfroid J,Cloeckaert A,Vergnaud G

    更新日期:2009-07-20 00:00:00

  • Susceptibility of Pediococcus isolates to antimicrobial compounds in relation to hop-resistance and beer-spoilage.

    abstract:BACKGROUND:Though important in the context of food microbiology and as potential pathogens in immuno-compromised humans, bacterial isolates belonging to the genus Pediococcus are best known for their association with contamination of ethanol fermentation processes (beer, wine, or fuel ethanol). Use of antimicrobial com...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-190

    authors: Haakensen M,Vickers DM,Ziola B

    更新日期:2009-09-07 00:00:00

  • Association of Bordetella dermonecrotic toxin with the extracellular matrix.

    abstract:BACKGROUND:Bordetella dermonecrotic toxin (DNT) causes the turbinate atrophy in swine atrophic rhinitis, caused by a Bordetella bronchiseptica infection of pigs, by inhibiting osteoblastic differentiation. The toxin is not actively secreted from the bacteria, and is presumed to be present in only small amounts in infec...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-247

    authors: Fukui-Miyazaki A,Kamitani S,Miyake M,Horiguchi Y

    更新日期:2010-09-25 00:00:00

  • Single stranded (ss)RNA-mediated antiviral response against infectious laryngotracheitis virus infection.

    abstract:BACKGROUND:Single stranded ribonucleic acid (ssRNA) binds to toll-like receptor (TLR)7 leading to recruitment of immune cells and production of pro-inflammatory cytokines, which has been shown in mammals. In chickens, synthetic ssRNA analog, resiquimod, has been shown to elicit antiviral response against infectious bur...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1398-6

    authors: Abdul-Cader MS,De Silva Senapathi U,Ahmed-Hassan H,Sharif S,Abdul-Careem MF

    更新日期:2019-02-08 00:00:00

  • A comparison of the Giardia lamblia trophozoite and cyst transcriptome using microarrays.

    abstract:BACKGROUND:Compared with many protists, Giardia lamblia has a simple life cycle alternating between cyst and trophozoite. Most research on the molecular biology of Giardia parasites has focused on trophozoites and the processes of excystation and encystation, whereas cysts have attracted less interest. The striking mor...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-11-91

    authors: Faghiri Z,Widmer G

    更新日期:2011-05-04 00:00:00

  • The role of iron uptake in pathogenicity and symbiosis in Photorhabdus luminescens TT01.

    abstract:BACKGROUND:Photorhabdus are Gram negative bacteria that are pathogenic to insect larvae whilst also having a mutualistic interaction with nematodes from the family Heterorhabditis. Iron is an essential nutrient and bacteria have different mechanisms for obtaining both the ferrous (Fe2+) and ferric (Fe3+) forms of this ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-177

    authors: Watson RJ,Millichap P,Joyce SA,Reynolds S,Clarke DJ

    更新日期:2010-06-22 00:00:00

  • Antibiotic resistance and adhesion properties of oral Enterococci associated to dental caries.

    abstract:BACKGROUND:Enterococci are increasingly associated with opportunistic infections in Humans but the role of the oral cavity as a reservoir for this species is unclear. This study aimed to explore the carriage rate of Enterococci in the oral cavity of Tunisian children and their antimicrobial susceptibility to a broad ra...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-11-155

    authors: Kouidhi B,Zmantar T,Mahdouani K,Hentati H,Bakhrouf A

    更新日期:2011-06-29 00:00:00

  • Release of extracellular ATP by bacteria during growth.

    abstract:BACKGROUND:Adenosine triphosphate (ATP) is used as an intracellular energy source by all living organisms. It plays a central role in the respiration and metabolism, and is the most important energy supplier in many enzymatic reactions. Its critical role as the energy storage molecule makes it extremely valuable to all...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-301

    authors: Mempin R,Tran H,Chen C,Gong H,Kim Ho K,Lu S

    更新日期:2013-12-24 00:00:00

  • Modelling invasive group A streptococcal disease using bioluminescence.

    abstract:BACKGROUND:The development of vaccines and evaluation of novel treatment strategies for invasive group A streptococcal (iGAS) disease requires suitable models of human infection that can be monitored longitudinally and are preferably non-invasive. Bio-photonic imaging provides an opportunity to reduce use of animals in...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1200-1

    authors: Lamb LE,Zhi X,Alam F,Pyzio M,Scudamore CL,Wiles S,Sriskandan S

    更新日期:2018-06-19 00:00:00

  • Safety evaluation of the antimicrobial peptide bovicin HC5 orally administered to a murine model.

    abstract:BACKGROUND:Bovicin HC5 is an antimicrobial peptide that shows a broad spectrum of activity and potential for biotechnological and therapeutic applications. To gain insight about the safety of bovicin HC5 application, the histological and immunostimulatory effects of orally administrated bovicin HC5 to BALB/c mice were ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-69

    authors: Paiva AD,Fernandes KM,Dias RS,Rocha Ados S,Oliveira LL,Neves CA,Paula SO,Mantovani HC

    更新日期:2013-03-27 00:00:00

  • Genotypic and phenotypic relatedness of Pseudomonas aeruginosa isolates among the major cystic fibrosis patient cohort in Italy.

    abstract:BACKGROUND:Pseudomonas aeruginosa is the predominant pathogen associated with the decline of pulmonary function in cystic fibrosis (CF) patients. Both environment-to-host acquisition and patient-to-patient transmission have been described for P. aeruginosa infection. Epidemic clones and bacterial phenotypic adaptation ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0760-1

    authors: Cigana C,Melotti P,Baldan R,Pedretti E,Pintani E,Iansa P,De Fino I,Favari F,Bergamini G,Tridello G,Cirillo DM,Assael BM,Bragonzi A

    更新日期:2016-07-11 00:00:00

  • Organization of DNA in a bacterial nucleoid.

    abstract:BACKGROUND:It is unclear how DNA is packaged in a bacterial cell in the absence of nucleosomes. To investigate the initial level of DNA condensation in bacterial nucleoid we used in vivo DNA digestion coupled with high-throughput sequencing of the digestion-resistant fragments. To this end, we transformed E. coli cells...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0637-3

    authors: Tolstorukov MY,Virnik K,Zhurkin VB,Adhya S

    更新日期:2016-02-20 00:00:00

  • Resistance to topoisomerase cleavage complex induced lethality in Escherichia coli via titration of transcription regulators PurR and FNR.

    abstract:BACKGROUND:Accumulation of gyrase cleavage complex in Escherichia coli from the action of quinolone antibiotics induces an oxidative damage cell death pathway. The oxidative cell death pathway has also been shown to be involved in the lethality following accumulation of cleavage complex formed by bacterial topoisomeras...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-11-261

    authors: Liu IF,Aedo S,Tse-Dinh YC

    更新日期:2011-12-12 00:00:00

  • Differential responses of osteoblasts and macrophages upon Staphylococcus aureus infection.

    abstract:BACKGROUND:Staphylococcus aureus (S. aureus) is one of the primary causes of bone infections which are often chronic and difficult to eradicate. Bacteria like S. aureus may survive upon internalization in cells and may be responsible for chronic and recurrent infections. In this study, we compared the responses of a ph...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-014-0207-5

    authors: Hamza T,Li B

    更新日期:2014-07-25 00:00:00

  • Genomic characterization of plant cell wall degrading enzymes and in silico analysis of xylanases and polygalacturonases of Fusarium virguliforme.

    abstract:BACKGROUND:Plant cell wall degrading enzymes (PCWDEs) are a subset of carbohydrate-active enzymes (CAZy) produced by plant pathogens to degrade plant cell walls. To counteract PCWDEs, plants release PCWDEs inhibitor proteins (PIPs) to reduce their impact. Several transgenic plants expressing exogenous PIPs that interac...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0761-0

    authors: Chang HX,Yendrek CR,Caetano-Anolles G,Hartman GL

    更新日期:2016-07-12 00:00:00

  • Hydrogen peroxide-producing pyruvate oxidase from Lactobacillus delbrueckii is catalytically activated by phosphotidylethanolamine.

    abstract:BACKGROUND:Pyruvate oxidase (Pox) is an important enzyme in bacterial metabolism for increasing ATP production and providing a fitness advantage via hydrogen peroxide production. However, few Pox enzymes have been characterized from bacterial species. The tetrameric non-hydrogen-peroxide producing Pox from E. coli is a...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01788-6

    authors: Cornacchione LP,Hu LT

    更新日期:2020-05-24 00:00:00

  • In vitro synergistic potentials of novel antibacterial combination therapies against Salmonella enterica serovar Typhimurium.

    abstract:BACKGROUND:The antibiotics generally used in farm animals are rapidly losing their effectiveness all over the world as bacteria develop antibiotic resistance. Like some other pathogenic bacteria multidrug-resistant strains of Salmonella enterica serovar Typhimurium (S. Typhimurium) are also frequently found in animals ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01810-x

    authors: Hossain MA,Park HC,Lee KJ,Park SW,Park SC,Kang J

    更新日期:2020-05-14 00:00:00

  • Critical aspects of using bacterial cell viability assays with the fluorophores SYTO9 and propidium iodide.

    abstract:BACKGROUND:Viability staining with SYTO9 and propidium iodide (PI) is a frequently used tool in microbiological studies. However, data generated by such routinely used method are often not critically evaluated for their accuracy. In this study we aim to investigate the critical aspects of this staining method using Sta...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0376-x

    authors: Stiefel P,Schmidt-Emrich S,Maniura-Weber K,Ren Q

    更新日期:2015-02-18 00:00:00

  • Difference in virulence between Neisseria meningitidis serogroups W and Y in transgenic mice.

    abstract:BACKGROUND:Neisseria meningitidis serogroups W and Y are the most common serogroups causing invasive meningococcal disease in Sweden. The majority of cases are caused by the serogroup W UK 2013 strain of clonal complex (cc) 11, and subtype 1 of the serogroup Y, YI strain of cc23. In this study, virulence factors of sev...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01760-4

    authors: Eriksson L,Stenmark B,Deghmane AE,Thulin Hedberg S,Säll O,Fredlund H,Mölling P,Taha MK

    更新日期:2020-04-15 00:00:00

  • Comparative analysis of the growth and biological activity of a respiratory and atheroma isolate of Chlamydia pneumoniae reveals strain-dependent differences in inflammatory activity and innate immune evasion.

    abstract:BACKGROUND:Chlamydia pneumoniae is a common human pathogen that is associated with upper and lower respiratory tract infections. It has also been suggested that C. pneumoniae infection can trigger or promote a number of chronic inflammatory conditions, including asthma and atherosclerosis. Several strains of C. pneumon...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0569-3

    authors: He X,Liang Y,LaValley MP,Lai J,Ingalls RR

    更新日期:2015-10-23 00:00:00

  • Purification and characterization of a surfactin-like molecule produced by Bacillus sp. H2O-1 and its antagonistic effect against sulfate reducing bacteria.

    abstract:BACKGROUND:Bacillus sp. H2O-1, isolated from the connate water of a Brazilian reservoir, produces an antimicrobial substance (denoted as AMS H2O-1) that is active against sulfate reducing bacteria, which are the major bacterial group responsible for biogenic souring and biocorrosion in petroleum reservoirs. Thus, the u...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-12-252

    authors: Korenblum E,de Araujo LV,Guimarães CR,de Souza LM,Sassaki G,Abreu F,Nitschke M,Lins U,Freire DM,Barreto-Bergter E,Seldin L

    更新日期:2012-11-07 00:00:00

  • Computational approaches for discovery of common immunomodulators in fungal infections: towards broad-spectrum immunotherapeutic interventions.

    abstract:BACKGROUND:Fungi are the second most abundant type of human pathogens. Invasive fungal pathogens are leading causes of life-threatening infections in clinical settings. Toxicity to the host and drug-resistance are two major deleterious issues associated with existing antifungal agents. Increasing a host's tolerance and...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-224

    authors: Kidane YH,Lawrence C,Murali TM

    更新日期:2013-10-07 00:00:00

  • CoBaltDB: Complete bacterial and archaeal orfeomes subcellular localization database and associated resources.

    abstract:BACKGROUND:The functions of proteins are strongly related to their localization in cell compartments (for example the cytoplasm or membranes) but the experimental determination of the sub-cellular localization of proteomes is laborious and expensive. A fast and low-cost alternative approach is in silico prediction, bas...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-88

    authors: Goudenège D,Avner S,Lucchetti-Miganeh C,Barloy-Hubler F

    更新日期:2010-03-23 00:00:00

  • Cow manure application effectively regulates the soil bacterial community in tea plantation.

    abstract:BACKGROUND:Cow manure is not only an agricultural waste, but also an organic fertilizer resource. The application of organic fertilizer is a feasible practice to mitigate the soil degradation caused by overuse of chemical fertilizers, which can affect the bacterial diversity and community composition in soils. However,...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01871-y

    authors: Zhang S,Sun L,Wang Y,Fan K,Xu Q,Li Y,Ma Q,Wang J,Ren W,Ding Z

    更新日期:2020-07-01 00:00:00

  • Diversity and phylogenetic analysis of endosymbiotic bacteria of the date palm root borer Oryctes agamemnon (Coleoptera: Scarabaeidae).

    abstract:BACKGROUND:The date palm root borer Oryctes agamemnon (Coleoptera: Scarabaeidae) is one of the major pests of palms. In Saudi Arabia, both larvae and adults of Oryctes are particularly troublesome, especially during the establishment of young date palm orchards. Endosymbiotic bacteria are known to have a key role in fo...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0422-8

    authors: El-Sayed WS,Ibrahim RA

    更新日期:2015-04-22 00:00:00

  • Verruculogen associated with Aspergillus fumigatus hyphae and conidia modifies the electrophysiological properties of human nasal epithelial cells.

    abstract:BACKGROUND:The role of Aspergillus fumigatus mycotoxins in the colonization of the respiratory tract by conidia has not been studied extensively, even though patients at risk from invasive aspergillosis frequently exhibit respiratory epithelium damage. In a previous study, we found that filtrates of A. fumigatus cultur...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-7-5

    authors: Khoufache K,Puel O,Loiseau N,Delaforge M,Rivollet D,Coste A,Cordonnier C,Escudier E,Botterel F,Bretagne S

    更新日期:2007-01-23 00:00:00

  • Application of fungal fluorescent staining in oral candidiasis: diagnostic analysis of 228 specimens.

    abstract:BACKGROUND:Several conventional methods, including fungal culture and periodic acid-Schiff (PAS) reagent staining, have been used to diagnose oral candidiasis. The aim of this study was to evaluate the efficacy of a novel method, fungal fluorescent staining, in relation to conventional protocols in the diagnosis of ora...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1467-x

    authors: Yao Y,Shi L,Zhang C,Sun H,Wu L

    更新日期:2019-05-14 00:00:00

  • Combined prime-boost vaccination against tick-borne encephalitis (TBE) using a recombinant vaccinia virus and a bacterial plasmid both expressing TBE virus non-structural NS1 protein.

    abstract:BACKGROUND:Heterologous prime-boost immunization protocols using different gene expression systems have proven to be successful tools in protecting against various diseases in experimental animal models. The main reason for using this approach is to exploit the ability of expression cassettes to prime or boost the immu...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-5-45

    authors: Aleshin SE,Timofeev AV,Khoretonenko MV,Zakharova LG,Pashvykina GV,Stephenson JR,Shneider AM,Altstein AD

    更新日期:2005-08-02 00:00:00

  • Comparative gene expression profiling in two congenic mouse strains following Bordetella pertussis infection.

    abstract:BACKGROUND:Susceptibility to Bordetella pertussis infection varies widely. These differences can partly be explained by genetic host factors. HcB-28 mice are more resistant to B. pertussis infection than C3H mice, which could partially be ascribed to the B. pertussis susceptibility locus-1 (Bps1) on chromosome 12. The ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-7-88

    authors: Banus S,Vandebriel RJ,Pennings JL,Gremmer ER,Wester PW,van Kranen HJ,Breit TM,Demant P,Mooi FR,Hoebee B,Kimman TG

    更新日期:2007-10-12 00:00:00

  • The role of interactions between bacterial chaperone, aspartate aminotransferase, and viral protein during virus infection in high temperature environment: the interactions between bacterium and virus proteins.

    abstract:BACKGROUND:The life cycle of a bacteriophage has tightly programmed steps to help virus infect its host through the interactions between the bacteriophage and its host proteins. However, bacteriophage-host protein interactions in high temperature environment remain poorly understood. To address this issue, the protein ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-48

    authors: Chen Y,Wei D,Wang Y,Zhang X

    更新日期:2013-02-26 00:00:00