Critical aspects of using bacterial cell viability assays with the fluorophores SYTO9 and propidium iodide.

abstract：BACKGROUND:The complete genome sequences of 44 Bacillus cereus group isolates collected from diverse sources in Japan were analyzed to determine their genetic backgrounds and diversity levels in Japan. Multilocus sequence typing (MLST) and core-genome single-nucleotide polymorphism (SNP) typing data from whole-genome s...

journal_title：BMC microbiology

authors： Okutani A,Inoue S,Noguchi A,Kaku Y,Morikawa S

更新日期：2019-12-16 00:00:00

abstract：BACKGROUND:It is well known that expression of certain bacterial genes responds rapidly to such stimuli as exposure to toxic chemicals and physical agents. It is generally believed that the proteins encoded in these genes are important for successful survival of the organism under the hostile conditions. Analogously, t...

journal_title：BMC microbiology

authors： Yamaguchi Y,Tomoyasu T,Takaya A,Morioka M,Yamamoto T

更新日期：2003-08-12 00:00:00

abstract：BACKGROUND:Brucellosis caused by Brucella abortus is one of the most important zoonoses in the world. Multiple-locus variable-number tandem repeat analysis (MLVA16) has been shown be a useful tool to epidemiological traceback studies in B. abortus infection. Thus, the present study aimed (i) to evaluate the genetic div...

journal_title：BMC microbiology

authors： Dorneles EM,Santana JA,Alves TM,Pauletti RB,Mol JP,Heinemann MB,Lage AP

更新日期：2014-07-11 00:00:00

abstract：BACKGROUND:Aspergillus fumigatus is a ubiquitous saprotrophic fungus and an opportunistic pathogen of humans and animals. Humans and animals can inhale hundreds of A. fumigatus spores daily. Normally this is harmless for humans, but in case of immunodeficiency, invasive pulmonary aspergillosis (IPA) can develop with a ...

journal_title：BMC microbiology

authors： Valdes ID,van den Berg J,Haagsman A,Escobar N,Meis JF,Hagen F,Haas PJ,Houbraken J,Wösten HAB,de Cock H

更新日期：2018-09-17 00:00:00

abstract：BACKGROUND:The Gifsy-I phage integrates into the Salmonella Typhimurium chromosome via an integrase mediated, site-specific recombination mechanism. Excision of the Gifsy-I phage requires three proteins, the Gifsy-I integrase (Int), the Gifsy-I excisionase (Xis) protein, and host encoded Integration Host Factor (IHF). ...

journal_title：BMC microbiology

authors： Flanigan A,Gardner J

更新日期：2008-11-17 00:00:00

abstract：BACKGROUND:Poorly understood but highly diverse microbial communities exist within anoxic and oxygen-depleted marine sediments. These communities often harbour single-celled eukaryotes that form symbiotic associations with different prokaryotes. During low tides in South-western British Columbia, Canada, vast areas of ...

journal_title：BMC microbiology

authors： Breglia SA,Yubuki N,Hoppenrath M,Leander BS

更新日期：2010-05-19 00:00:00

abstract：BACKGROUND:Candida krusei has been known to exhibit communal interactions such as pellicle formation and crawling out of nutritional broth. We noticed another possible interaction on agar surfaces, where C. krusei yeast cells formed mycelia along agar surfaces toward each other. We report here the results of experiment...

journal_title：BMC microbiology

authors： Fleischmann J,Broeckling CD,Lyons S

更新日期：2017-03-11 00:00:00

abstract：BACKGROUND:Participation of renal cells in the pathogenesis of staphylococcal enterotoxin B (SEB) is critical for late cleansing and sequestration of the antigens facilitated by CD1d mediated antigen sensing and recognition. This is a noted deviation from the typical antigen recognition process that recruits the major ...

journal_title：BMC microbiology

authors： Hammamieh R,Chakraborty N,Lin Y,Shupp JW,Miller SA,Morris S,Jett M

更新日期：2015-02-04 00:00:00

abstract：BACKGROUND:Affecting the core functional microbiome, peculiar high level taxonomic unbalances of the human intestinal microbiota have been recently associated with specific diseases, such as obesity, inflammatory bowel diseases, and intestinal inflammation. RESULTS:In order to specifically monitor microbiota unbalance...

journal_title：BMC microbiology

authors： Candela M,Consolandi C,Severgnini M,Biagi E,Castiglioni B,Vitali B,De Bellis G,Brigidi P

更新日期：2010-04-19 00:00:00

abstract：ERRATUM:Upon publication of the original article (1) it was highlighted by the authors that a grant awarded to support the research work of the study was missed in the acknowledgements. It should also be acknowledged that the grant titled "Genotyping and Molecular Epidemiological Characteristic of Bacillus anthracis in...

journal_title：BMC microbiology

authors： Li S,Ma Q,Chen H,Wang D,Liu Y,Wei X,You L,Yao G,Tian K,Tang G

更新日期：2018-04-12 00:00:00

abstract：BACKGROUND:The symbiotic bacteria associated with edible fungi are valuable microbial resources worthy of in-depth exploration. It is important to analyze the community structure and succession of symbiotic bacteria in mushrooms. This can assist in the isolation of growth-promoting strains that have an essential relati...

journal_title：BMC microbiology

authors： Sun S,Li F,Xu X,Liu Y,Kong X,Chen J,Liu T,Chen L

更新日期：2020-10-14 00:00:00

abstract：BACKGROUND:Sigma factors are one of the components of RNA polymerase holoenzymes, and an essential factor of transcription initiation in bacteria. Corynebacterium glutamicum possesses seven genes coding for sigma factors, most of which have been studied to some detail; however, the role of SigD in transcriptional regul...

journal_title：BMC microbiology

authors： Taniguchi H,Busche T,Patschkowski T,Niehaus K,Pátek M,Kalinowski J,Wendisch VF

更新日期：2017-07-12 00:00:00

abstract：BACKGROUND:Tetrahymena thermophila is one of the best characterized unicellular eukaryotes and its genome is sequenced in its entirety. However, the AT-richness of the genome and an unusual codon usage cause problems in cloning and expression of the ciliate DNA. To overcome these technical hiatuses we developed a Cre-d...

journal_title：BMC microbiology

authors： Weide T,Bockau U,Rave A,Herrmann L,Hartmann MW

更新日期：2007-03-01 00:00:00

abstract：BACKGROUND:ClpP is important for bacterial growth and plays an indispensable role in cellular protein quality control systems by refolding or degrading damaged proteins, but the physiological significance of ClpP in Enterococcus faecalis remains obscure. A clpP deletion mutant (△clpP) was constructed using the E. faeca...

journal_title：BMC microbiology

authors： Zheng J,Wu Y,Lin Z,Wang G,Jiang S,Sun X,Tu H,Yu Z,Qu D

更新日期：2020-02-07 00:00:00

abstract：BACKGROUND:Propionibacterium acnes is a commensal of human skin but is also known to be involved in certain diseases, such as acne vulgaris and infections of orthopaedic implants. Treatment of these conditions is complicated by increased resistance to antibiotics and/or biofilm formation of P. acnes bacteria. P. acnes ...

journal_title：BMC microbiology

authors： Lood R,Mörgelin M,Holmberg A,Rasmussen M,Collin M

更新日期：2008-08-15 00:00:00

abstract：BACKGROUND:The phytohormone indole-3-acetic acid (IAA) is widely distributed among plant-associated bacteria. Certain strains of the Pseudomonas syringae complex can further metabolize IAA into a less biologically active amino acid conjugate, 3-indole-acetyl-ε-L-lysine, through the action of the iaaL gene. In P. syring...

journal_title：BMC microbiology

authors： Castillo-Lizardo MG,Aragón IM,Carvajal V,Matas IM,Pérez-Bueno ML,Gallegos MT,Barón M,Ramos C

更新日期：2015-08-19 00:00:00

abstract：BACKGROUND:The gut comprises an essential barrier that protects both invertebrate and vertebrate animals from invasion by microorganisms. Disruption of the balanced relationship between indigenous gut microbiota and their host can result in gut bacteria eliciting host responses similar to those caused by invasive patho...

journal_title：BMC microbiology

authors： Broderick NA,Raffa KF,Handelsman J

更新日期：2010-04-27 00:00:00

abstract：BACKGROUND:Vibrio parahaemolyticus is a food-borne pathogen causing inflammation of the gastrointestinal epithelium. Pathogenic strains of this bacterium possess two Type III Secretion Systems (TTSS) that deliver effector proteins into host cells. In order to better understand human host cell responses to V. parahaemol...

journal_title：BMC microbiology

authors： Matlawska-Wasowska K,Finn R,Mustel A,O'Byrne CP,Baird AW,Coffey ET,Boyd A

更新日期：2010-12-30 00:00:00

abstract：BACKGROUND:Most of the studies evaluating the secular trends of blood isolates come from tertiary hospitals in urban areas. We sought to study the trends of the antimicrobial resistance of blood isolates in patients from a rural population hospitalized in a tertiary hospital in a small city in Greece. METHODS:We retro...

journal_title：BMC microbiology

authors： Falagas ME,Bakossi A,Pappas VD,Holevas PV,Bouras A,Stamata E

更新日期：2006-05-02 00:00:00

abstract：BACKGROUND:Members of the genus Bifidobacterium are anaerobic Gram-positive Actinobacteria, which are natural inhabitants of human and animal gastrointestinal tract. Certain bifidobacteria are frequently used as food additives and probiotic pharmaceuticals, because of their various health-promoting properties. Due to t...

journal_title：BMC microbiology

authors： Jarocki P,Podleśny M,Komoń-Janczara E,Kucharska J,Glibowska A,Targoński Z

更新日期：2016-07-22 00:00:00

abstract：BACKGROUND:Bacteria release a wide variety of small molecules including cell-to-cell signaling compounds. Gram-negative bacteria use a variety of self-produced autoinducers such as acylated homoserine lactones (acyl-HSLs) as signal compounds for quorum sensing (QS) within and between bacterial species. QS plays a signi...

journal_title：BMC microbiology

authors： Minagawa S,Inami H,Kato T,Sawada S,Yasuki T,Miyairi S,Horikawa M,Okuda J,Gotoh N

更新日期：2012-05-10 00:00:00

abstract：BACKGROUND:Malic enzymes decarboxylate the tricarboxylic acid (TCA) cycle intermediate malate to the glycolytic end-product pyruvate and are well positioned to regulate metabolic flux in central carbon metabolism. Despite the wide distribution of these enzymes, their biological roles are unclear in part because the rea...

journal_title：BMC microbiology

authors： Zhang Y,Smallbone LA,diCenzo GC,Morton R,Finan TM

更新日期：2016-07-26 00:00:00

abstract：BACKGROUND:Chlamydiae are obligate intracellular pathogens that possess a type III secretion system to deliver proteins into the host cell during infection. Small molecule inhibitors of type III secretion in Yersinia, termed INPs (Innate Pharmaceuticals AB) were reported to strongly inhibit Chlamydia growth in epitheli...

journal_title：BMC microbiology

authors： Muschiol S,Normark S,Henriques-Normark B,Subtil A

更新日期：2009-04-21 00:00:00

abstract：BACKGROUND:During the last two decades research on animal filarial parasites, especially Onchocerca ochengi, infecting cattle in savanna areas of Africa revealed that O. ochengi as an animal model has biological features that are similar to those of O. volvulus, the aetiological agent of human onchocerciasis. There is,...

journal_title：BMC microbiology

authors： Ngwasiri NN,Brattig NW,Ndjonka D,Liebau E,Paguem A,Leusder D,Kingsley MT,Eisenbarth A,Renz A,Daniel AM

更新日期：2021-01-06 00:00:00

abstract：BACKGROUND:Establishing an optimal proportion of nitrifying microbial populations, including ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB), complete nitrite oxidizers (comammox) and ammonia-oxidizing archaea (AOA), is important for ensuring the efficiency of nitrification in water treatment systems...

journal_title：BMC microbiology

authors： Scarascia G,Cheng H,Harb M,Hong PY

更新日期：2017-04-04 00:00:00

abstract：BACKGROUND:Photorhabdus are Gram negative bacteria that are pathogenic to insect larvae whilst also having a mutualistic interaction with nematodes from the family Heterorhabditis. Iron is an essential nutrient and bacteria have different mechanisms for obtaining both the ferrous (Fe2+) and ferric (Fe3+) forms of this ...

journal_title：BMC microbiology

authors： Watson RJ,Millichap P,Joyce SA,Reynolds S,Clarke DJ

更新日期：2010-06-22 00:00:00

abstract：BACKGROUND:Diazotrophic (nitrogen-fixing) Gram-positive and endospore-formed Paenibacillus spp. have potential uses as a bacterial fertilizer in agriculture. The transcriptional analysis of nitrogen fixation in Paenibacillus is lacking, although regulation mechanisms of nitrogen fixation have been well studied in Gram-...

journal_title：BMC microbiology

authors： Shi HW,Wang LY,Li XX,Liu XM,Hao TY,He XJ,Chen SF

更新日期：2016-03-01 00:00:00

abstract：BACKGROUND:Avian pathogenic Escherichia coli (APEC) and uropathogenic E. coli (UPEC) are the two main subsets of extraintestinal pathogenic E. coli (ExPEC). Both types have multiple iron acquisition systems, including heme and siderophores. Although iron transport systems involved in the pathogenesis of APEC or UPEC ha...

journal_title：BMC microbiology

authors： Gao Q,Wang X,Xu H,Xu Y,Ling J,Zhang D,Gao S,Liu X

更新日期：2012-07-20 00:00:00

abstract：BACKGROUND:Since 1994, Brucella strains have been isolated from a wide range of marine mammals. They are currently recognized as two new Brucella species, B. pinnipedialis for the pinniped isolates and B. ceti for the cetacean isolates in agreement with host preference and specific phenotypic and molecular markers. In ...

journal_title：BMC microbiology

authors： Maquart M,Le Flèche P,Foster G,Tryland M,Ramisse F,Djønne B,Al Dahouk S,Jacques I,Neubauer H,Walravens K,Godfroid J,Cloeckaert A,Vergnaud G

更新日期：2009-07-20 00:00:00

abstract：BACKGROUND:Ustilago esculenta, a typical dimorphic fungus could infect Zizania latifolia and induce host stem swollen to form an edible vegetable called Jiaobai in China. The strains differentiation especially in the mating ability and pathogenicity is closely related to different phenotypes of Jiaobai formed in the fi...

journal_title：BMC microbiology

authors： Zhang Y,Wu M,Ge Q,Yang M,Xia W,Cui H,Yu X,Zhang S,Ye Z

更新日期：2019-09-05 00:00:00