Abstract:
BACKGROUND:Photorhabdus are Gram negative bacteria that are pathogenic to insect larvae whilst also having a mutualistic interaction with nematodes from the family Heterorhabditis. Iron is an essential nutrient and bacteria have different mechanisms for obtaining both the ferrous (Fe2+) and ferric (Fe3+) forms of this metal from their environments. In this study we were interested in analyzing the role of Fe3+ and Fe2+ iron uptake systems in the ability of Photorhabdus to interact with its invertebrate hosts. RESULTS:We constructed targeted deletion mutants of exbD, feoABC and yfeABCD in P. luminescens TT01. The exbD mutant was predicted to be crippled in its ability to obtain Fe3+ and we show that this mutant does not grow well in iron-limited media. We also show that this mutant was avirulent to the insect but was unaffected in its symbiotic interaction with Heterorhabditis. Furthermore we show that a mutation in feoABC (encoding a predicted Fe2+ permease) was unaffected in both virulence and symbiosis whilst the divalent cation transporter encoded by yfeABCD is required for virulence in the Tobacco Hornworm, Manduca sexta (Lepidoptera) but not in the Greater Wax Moth, Galleria mellonella (Lepidoptera). Moreover the Yfe transporter also appears to have a role during colonization of the IJ stage of the nematode. CONCLUSION:In this study we show that iron uptake (via the TonB complex and the Yfe transporter) is important for the virulence of P. luminescens to insect larvae. Moreover this study also reveals that the Yfe transporter appears to be involved in Mn2+-uptake during growth in the gut lumen of the IJ nematode. Therefore, the Yfe transporter in P. luminescens TT01 is important during colonization of both the insect and nematode and, moreover, the metal ion transported by this pathway is host-dependent.
journal_name
BMC Microbioljournal_title
BMC microbiologyauthors
Watson RJ,Millichap P,Joyce SA,Reynolds S,Clarke DJdoi
10.1186/1471-2180-10-177subject
Has Abstractpub_date
2010-06-22 00:00:00pages
177issn
1471-2180pii
1471-2180-10-177journal_volume
10pub_type
杂志文章相关文献
BMC MICROBIOLOGY文献大全abstract:BACKGROUND:There is a rapid emergence of multiple resistant gram-negative bacteria due to overuse of antibiotics in the treatment of infections. Biofilms consist of polymicrobial communities that survive the host's defense system. The key bacteria in biofilms are slow growing and support an attachment and rapid growth ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0821-5
更新日期:2016-09-09 00:00:00
abstract:BACKGROUND:Bacteria adapted to live within animals can protect their hosts against harmful infections. Beyond antagonism with pathogens, a 'defensive' bacterial symbiont could engage in additional interactions with other colonizing micro-organisms. A single bacterium might thus have cascading ecological impacts on the ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01845-0
更新日期:2020-06-15 00:00:00
abstract:BACKGROUND:A growing body of evidence suggests that Mycobacterium tuberculosis (Mtb) uses the host's cholesterol as a source of carbon and energy during infection. Strains defective in cholesterol transport or degradation exhibit attenuated growth in activated macrophages and diminished infectivity in animal models. Th...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-43
更新日期:2013-02-20 00:00:00
abstract:BACKGROUND:Nitric oxide (NO) is produced as part of the host immune response to bacterial infections, including urinary tract infections. The enzyme flavohemoglobin, coded by the hmp gene, is involved in protecting bacterial cells from the toxic effects of NO and represents a potentially interesting target for developm...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-14-65
更新日期:2014-03-14 00:00:00
abstract:BACKGROUND:Listeria monocytogenes has been implicated in several food borne outbreaks as well as sporadic cases of disease. Increased understanding of the biology of this organism is important in the prevention of food borne listeriosis. The infectivity of Listeria monocytogenes ScottA, cultivated with and without oxyg...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-7-55
更新日期:2007-06-14 00:00:00
abstract:BACKGROUND:Anthrax, the zoonotic disease caused by the gram-positive bacterium Bacillus anthracis, is nowadays rare in northern parts of Europe including Finland and Scandinavia. Only two minor outbreaks of anthrax in 1988 and in 2004 and one sporadic infection in 2008 have been detected in animals in Finland since the...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1250-4
更新日期:2018-09-03 00:00:00
abstract:BACKGROUND:The role of Aspergillus fumigatus mycotoxins in the colonization of the respiratory tract by conidia has not been studied extensively, even though patients at risk from invasive aspergillosis frequently exhibit respiratory epithelium damage. In a previous study, we found that filtrates of A. fumigatus cultur...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-7-5
更新日期:2007-01-23 00:00:00
abstract:BACKGROUND:In this study, we have evaluated the immunological status of hepatitis C virus (HCV)-infected patients aiming at identifying putative biomarkers associated with distinct degrees of liver fibrosis. Peripheral blood and tissue T-cells as well as cytokine levels were quantified by flow cytometry. RESULTS:Data ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0610-6
更新日期:2016-01-07 00:00:00
abstract:BACKGROUND:Legionella pneumophila, the causative agent of Legionnaire's disease, may enter a viable but non-culturable (VBNC) state triggered by environmental stress conditions. Specific outer-membrane epitopes of L. pneumophila are used in many diagnostic applications and some of them are linked to important virulence...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1220-x
更新日期:2018-07-17 00:00:00
abstract:BACKGROUND:The increase in resistance to antibiotics among disease-causing bacteria necessitates the development of alternative antimicrobial approaches such as the use of light-activated antimicrobial agents (LAAAs). Light of an appropriate wavelength activates the LAAA to produce cytotoxic species which can then caus...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-111
更新日期:2008-07-01 00:00:00
abstract:BACKGROUND:Alleviating arsenic (As) contamination is a high-priority environmental issue. Hyperaccumulator plants may harbor endophytic bacteria able to detoxify As. Therefore, we investigated the distribution, diversity, As (III) resistance levels, and resistance-related functional genes of arsenite-resistant bacteria...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1184-x
更新日期:2018-05-08 00:00:00
abstract:BACKGROUND:Wolbachia, one of the most abundant taxa of intracellular Alphaproteobacteria, is widespread among arthropods and filarial nematodes. The presence of these maternally inherited bacteria is associated with modifications of host fitness, including a variety of reproductive abnormalities, such as cytoplasmic in...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1652-y
更新日期:2019-12-24 00:00:00
abstract:BACKGROUND:Viability staining with SYTO9 and propidium iodide (PI) is a frequently used tool in microbiological studies. However, data generated by such routinely used method are often not critically evaluated for their accuracy. In this study we aim to investigate the critical aspects of this staining method using Sta...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0376-x
更新日期:2015-02-18 00:00:00
abstract:BACKGROUND:Most studies on the human microbiota have analyzed stool samples, although a large proportion of the absorption of nutrients takes place in upper gut tract. We collected samples from different locations along the entire gastrointestinal tract from six patients who had simultaneously undergone upper endoscopy...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1304-7
更新日期:2018-10-24 00:00:00
abstract:BACKGROUND:Biofilm is a fundamental bacterial survival mode which proceeds through three main generalized phases: adhesion, maturation, and dispersion. Lactobacilli spp. (LB) are critical components of gut and reproductive health and are widely used probiotics. Evaluation of time-dependent mechanisms of biofilm formati...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01753-3
更新日期:2020-03-24 00:00:00
abstract:BACKGROUND:Brucellosis incidence in China is divided into three stages: high incidence (1950s-1960s), decline (1970s-1980s), and re-emergence (1990s-2010s). At the re-emergence stage, Brucellosis incidence grew exponentially and spread to all 32 provinces. We describe the magnitude and the etiological distribution chan...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1149-0
更新日期:2018-01-10 00:00:00
abstract:BACKGROUND:The human gastrointestinal (GI) tract microbiota has been a subject of intense research throughout the 3rd Millennium. Now that a general picture about microbiota composition in health and disease is emerging, questions about factors determining development of microbiotas with specific community structures w...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1538-z
更新日期:2019-07-18 00:00:00
abstract:BACKGROUND:Most group A streptococcal (GAS) vaccine strategies have focused on the surface M protein, a major virulence factor of GAS. The amino-terminus of the M protein elicits antibodies, that are both opsonic and protective, but which are type specific. J14, a chimeric peptide that contains 14 amino acids from the ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-6-71
更新日期:2006-08-09 00:00:00
abstract:BACKGROUND:Whole-genome sequencing using high throughput technologies has revolutionized and speeded up the scientific investigation of bacterial genetics, biochemistry, and molecular biology. Lactic acid bacteria (LABs) have been extensively used in fermentation and more recently as probiotics in food products that pr...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01980-8
更新日期:2020-10-23 00:00:00
abstract:BACKGROUND:Invasive aspergillosis (IA) is a major cause of infectious morbidity and mortality in immune compromised patients. Studies on the pathogenesis of IA have been limited by the difficulty to monitor disease progression in real-time. For real-time monitoring of the infection, we recently engineered a bioluminesc...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-105
更新日期:2010-04-08 00:00:00
abstract:BACKGROUND:Quorum sensing is a communication system that regulates gene expression in response to population density and often regulates virulence determinants. Deletion of the luxR homologue vjbR highly attenuates intracellular survival of Brucella melitensis and has been interpreted to be an indication of a role for ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-167
更新日期:2010-06-08 00:00:00
abstract:BACKGROUND:Recent scientific developments have shed more light on the importance of the host-microbe interaction, particularly in the gut. However, the mechanistic study of the host-microbe interplay is complicated by the intrinsic limitations in reaching the different areas of the gastrointestinal tract (GIT) in vivo....
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-14-133
更新日期:2014-05-22 00:00:00
abstract:BACKGROUND:Tetrahymena thermophila is one of the best characterized unicellular eukaryotes and its genome is sequenced in its entirety. However, the AT-richness of the genome and an unusual codon usage cause problems in cloning and expression of the ciliate DNA. To overcome these technical hiatuses we developed a Cre-d...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-7-12
更新日期:2007-03-01 00:00:00
abstract:BACKGROUND:Klebsiella pneumoniae is an important gram-negative opportunistic pathogen causing primarily urinary tract infections, respiratory infections, and bacteraemia. The ability of bacteria to form biofilms on medical devices, e.g. catheters, has a major role in development of many nosocomial infections. Most clin...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-179
更新日期:2010-06-23 00:00:00
abstract:BACKGROUND:Understanding mollicutes is challenging due to their variety and relationship with host cells. Invasion has explained issues related to their opportunistic role. Few studies have been done on the Ureaplasma diversum mollicute, which is detected in healthy or diseased bovine. The invasion in Hep-2 cells of fo...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-83
更新日期:2010-03-17 00:00:00
abstract:BACKGROUND:Mycoplasma pneumoniae has previously been characterized as a micro-organism that is genetically highly stable. In spite of this genetic stability, homologous DNA recombination has been hypothesized to lie at the basis of antigenic variation of the major surface protein, P1, of M. pneumoniae. In order to iden...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-167
更新日期:2008-10-02 00:00:00
abstract:BACKGROUND:Ophiocordyceps sinensis (DongChong XiaCao (DCXC) in Chinese), a fungal parasite of caterpillars, is a traditional Chinese medicine. Bioactive components isolated from natural DCXC possess a wide range of pharmacological actions. Many efforts have been directed towards isolating the fungi based on culture-dep...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0813-5
更新日期:2016-08-26 00:00:00
abstract:BACKGROUND:The genus Phytophthora includes a group of agriculturally important pathogens and they are commonly regarded as water molds. They produce motile zoospores that can move via water currents and on their own locomotion in aquatic environments. However, zoosporic response to dissolved oxygen, an important water ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-14-124
更新日期:2014-05-13 00:00:00
abstract:BACKGROUND:Rhodosporidium toruloides is a β-carotenoid accumulating, oleaginous yeast that has great biotechnological potential. The lack of reliable and efficient genetic manipulation tools have been a major hurdle blocking its adoption as a biotechnology platform. RESULTS:We report for the first time the development...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-14-50
更新日期:2014-02-27 00:00:00
abstract:BACKGROUND:The RNA interference (RNAi) pathway acts as an innate antiviral immune response in Aedes aegypti, modulating arbovirus infection of mosquitoes. Sindbis virus (SINV; family: Togaviridae, genus: Alphavirus) is an arbovirus that infects Ae. aegypti in the laboratory. SINV strain TR339 encounters a midgut escape...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-130
更新日期:2010-04-28 00:00:00