Cloning and disruption of the UeArginase in Ustilago esculenta: evidence for a role of arginine in its dimorphic transition.

Abstract:

BACKGROUND:Ustilago esculenta, a typical dimorphic fungus could infect Zizania latifolia and induce host stem swollen to form an edible vegetable called Jiaobai in China. The strains differentiation especially in the mating ability and pathogenicity is closely related to different phenotypes of Jiaobai formed in the fields. Dimorphic switching, a tightly regulated processes, is essential for the pathogenetic development of dimorphic fungi. In responses to environment cues, dimorphic switching can be activated through two conserved cell signaling pathways-PKA and MAPK pathways. Previous study indicated that exogenous arginine could induce hyphal formation in several dimorphic fungi through hydrolysis by arginase, but inhibit the dimorphic transition of U. esculenta. We conducted this study to reveal the function of arginine on dimorphic transition of U. esculenta. RESULTS:In this study, we found that arginine, but not its anabolites, could slow down the dimorphic transition of U. esculenta proportionally to the concentration of arginine. Besides, UeArginase, predicated coding arginase in U. esculenta was cloned and characterized. UeArginase mutants could actually increase the content of endogenous arginine, and slow down the dimorphic transition on either nutritious rich or poor medium. Either adding exogenous arginine or UeArginase deletion lead to down regulated expressions of UePkaC, UePrf1, mfa1.2, mfa2.1, pra1 and pra2, along with an increased content of arginine during mating process. CONCLUSION:Results of this study indicated a direct role of arginine itself on the inhibition of dimorphic transition of U. esculenta, independent of its hydrolysis by UeArginase.

journal_name

BMC Microbiol

journal_title

BMC microbiology

authors

Zhang Y,Wu M,Ge Q,Yang M,Xia W,Cui H,Yu X,Zhang S,Ye Z

doi

10.1186/s12866-019-1588-2

subject

Has Abstract

pub_date

2019-09-05 00:00:00

pages

208

issue

1

issn

1471-2180

pii

10.1186/s12866-019-1588-2

journal_volume

19

pub_type

杂志文章
  • Characterization of lpaH2 gene corresponding to lipopeptide synthesis in Bacillus amyloliquefaciens HAB-2.

    abstract:BACKGROUND:Bacillus spp. have prominent ability to suppress plant pathogens and corresponding diseases. Previous analyses of Bacillus spp. revealed numerous gene clusters involved in nonribosomal synthesis of cyclic lipopeptides with distinct antimicrobial action. The 4'-phosphopantetheinyl transferase (PPTase) encoded...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-017-1134-z

    authors: Jin P,Wang H,Liu W,Miao W

    更新日期:2017-12-04 00:00:00

  • Characterization of a filamentous biofilm community established in a cellulose-fed microbial fuel cell.

    abstract:BACKGROUND:Microbial fuel cells (MFCs) are devices that exploit microorganisms to generate electric power from organic matter. Despite the development of efficient MFC reactors, the microbiology of electricity generation remains to be sufficiently understood. RESULTS:A laboratory-scale two-chamber microbial fuel cell ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-6

    authors: Ishii S,Shimoyama T,Hotta Y,Watanabe K

    更新日期:2008-01-10 00:00:00

  • Safety evaluation of the antimicrobial peptide bovicin HC5 orally administered to a murine model.

    abstract:BACKGROUND:Bovicin HC5 is an antimicrobial peptide that shows a broad spectrum of activity and potential for biotechnological and therapeutic applications. To gain insight about the safety of bovicin HC5 application, the histological and immunostimulatory effects of orally administrated bovicin HC5 to BALB/c mice were ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-69

    authors: Paiva AD,Fernandes KM,Dias RS,Rocha Ados S,Oliveira LL,Neves CA,Paula SO,Mantovani HC

    更新日期:2013-03-27 00:00:00

  • Optimized molecular resolution of cross-contamination alerts in clinical mycobacteriology laboratories.

    abstract:BACKGROUND:The phenomenon of misdiagnosing tuberculosis (TB) by laboratory cross-contamination when culturing Mycobacterium tuberculosis (MTB) has been widely reported and it has an obvious clinical, therapeutic and social impact. The final confirmation of a cross-contamination event requires the molecular identificati...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-30

    authors: Martín A,Herranz M,Lirola MM,Fernández RF,INDAL-TB group.,Bouza E,García de Viedma D

    更新日期:2008-02-14 00:00:00

  • Altered expression of cell cycle and apoptotic proteins in chronic hepatitis C virus infection.

    abstract:BACKGROUND:A disrupted cell cycle progression of hepatocytes was reported in chronic hepatitis C virus (HCV) infection, which can contribute significantly in the associated pathogenesis. The present study aimed to further elaborate these disruptions by evaluating the expression of key cell cycle and apoptotic proteins ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-133

    authors: Sarfraz S,Hamid S,Siddiqui A,Hussain S,Pervez S,Alexander G

    更新日期:2008-08-05 00:00:00

  • Isocitrate dehydrogenase mutation in Vibrio anguillarum results in virulence attenuation and immunoprotection in rainbow trout (Oncorhynchus mykiss).

    abstract:BACKGROUND:Vibrio anguillarum is an extracellular bacterial pathogen that is a causative agent of vibriosis in finfish and crustaceans with mortality rates ranging from 30% to 100%. Mutations in central metabolism (glycolysis and the TCA cycle) of intracellular pathogens often result in attenuated virulence due to depl...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-017-1124-1

    authors: Mou X,Spinard EJ,Hillman SL,Nelson DR

    更新日期:2017-11-14 00:00:00

  • Chlamydia trachomatis responds to heat shock, penicillin induced persistence, and IFN-gamma persistence by altering levels of the extracytoplasmic stress response protease HtrA.

    abstract:BACKGROUND:Chlamydia trachomatis, an obligate intracellular human pathogen, is the most prevalent bacterial sexually transmitted infection worldwide and a leading cause of preventable blindness. HtrA is a virulence and stress response periplasmic serine protease and molecular chaperone found in many bacteria. Recombina...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-190

    authors: Huston WM,Theodoropoulos C,Mathews SA,Timms P

    更新日期:2008-11-06 00:00:00

  • Diversity and phylogenetic analysis of endosymbiotic bacteria of the date palm root borer Oryctes agamemnon (Coleoptera: Scarabaeidae).

    abstract:BACKGROUND:The date palm root borer Oryctes agamemnon (Coleoptera: Scarabaeidae) is one of the major pests of palms. In Saudi Arabia, both larvae and adults of Oryctes are particularly troublesome, especially during the establishment of young date palm orchards. Endosymbiotic bacteria are known to have a key role in fo...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0422-8

    authors: El-Sayed WS,Ibrahim RA

    更新日期:2015-04-22 00:00:00

  • Gene expression profiling of Mycobacterium avium subsp. paratuberculosis in simulated multi-stress conditions and within THP-1 cells reveals a new kind of interactive intramacrophage behaviour.

    abstract:BACKGROUND:Recent studies have identified in Mycobacterium avium subsp. paratuberculosis (MAP), already known as a pathogen in ruminants, a potential zoonotic agent of some autoimmune diseases in humans. Therefore, considering the possible risk for public health, it is necessary a thorough understanding of MAP's gene e...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-12-87

    authors: Cossu A,Sechi LA,Zanetti S,Rosu V

    更新日期:2012-05-30 00:00:00

  • Genotyping of Chlamydophila psittaci using a new DNA microarray assay based on sequence analysis of ompA genes.

    abstract:BACKGROUND:The currently used genotyping system for the avian zoonotic pathogen Chlamydophila (C.) psittaci has evolved from serology and is based on ompA sequence variations. It includes seven avian and two non-avian genotypes. Restriction enzyme cleavage of the amplified ompA gene and, less frequently, ompA sequencin...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-63

    authors: Sachse K,Laroucau K,Hotzel H,Schubert E,Ehricht R,Slickers P

    更新日期:2008-04-17 00:00:00

  • Bactericidal activity of M protein conserved region antibodies against group A streptococcal isolates from the Northern Thai population.

    abstract:BACKGROUND:Most group A streptococcal (GAS) vaccine strategies have focused on the surface M protein, a major virulence factor of GAS. The amino-terminus of the M protein elicits antibodies, that are both opsonic and protective, but which are type specific. J14, a chimeric peptide that contains 14 amino acids from the ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-6-71

    authors: Yoonim N,Olive C,Pruksachatkunakorn C,Pruksakorn S

    更新日期:2006-08-09 00:00:00

  • A novel gene expression system for Ralstonia eutropha based on the T7 promoter.

    abstract:BACKGROUND:Ralstonia eutropha (syn. Cupriavidus necator) is a model microorganism for studying metabolism of polyhydroxyalkanoates (PHAs) and a potential chassis for protein expression due to various advantages. Although current plasmid systems of R. eutropha provide a basic platform for gene expression, the performanc...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01812-9

    authors: Hu M,Xiong B,Li Z,Liu L,Li S,Zhang C,Zhang X,Bi C

    更新日期:2020-05-19 00:00:00

  • Susceptibility of Pediococcus isolates to antimicrobial compounds in relation to hop-resistance and beer-spoilage.

    abstract:BACKGROUND:Though important in the context of food microbiology and as potential pathogens in immuno-compromised humans, bacterial isolates belonging to the genus Pediococcus are best known for their association with contamination of ethanol fermentation processes (beer, wine, or fuel ethanol). Use of antimicrobial com...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-190

    authors: Haakensen M,Vickers DM,Ziola B

    更新日期:2009-09-07 00:00:00

  • Comparative diversity of microbiomes and Resistomes in beef feedlots, downstream environments and urban sewage influent.

    abstract:BACKGROUND:Comparative knowledge of microbiomes and resistomes across environmental interfaces between animal production systems and urban settings is lacking. In this study, we executed a comparative analysis of the microbiota and resistomes of metagenomes from cattle feces, catch basin water, manured agricultural soi...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1548-x

    authors: Zaheer R,Lakin SM,Polo RO,Cook SR,Larney FJ,Morley PS,Booker CW,Hannon SJ,Van Domselaar G,Read RR,McAllister TA

    更新日期:2019-08-27 00:00:00

  • Escherichia coli phylogenetic group determination and its application in the identification of the major animal source of fecal contamination.

    abstract:BACKGROUND:Escherichia coli strains are commonly found in the gut microflora of warm-blooded animals. These strains can be assigned to one of the four main phylogenetic groups, A, B1, B2 and D, which can be divided into seven subgroups (A0, A1, B1, B22, B23, D1 and D2), according to the combination of the three genetic...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-161

    authors: Carlos C,Pires MM,Stoppe NC,Hachich EM,Sato MI,Gomes TA,Amaral LA,Ottoboni LM

    更新日期:2010-06-01 00:00:00

  • Kinin release from human kininogen by 10 aspartic proteases produced by pathogenic yeast Candida albicans.

    abstract:BACKGROUND:Candida albicans yeast produces 10 distinct secreted aspartic proteases (Saps), which are some of the most important virulence factors of this pathogenic fungus. One of the suggested roles of Saps is their deregulating effect on various proteolytic cascades that constitute the major homeostatic systems in hu...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0394-8

    authors: Kozik A,Gogol M,Bochenska O,Karkowska-Kuleta J,Wolak N,Kamysz W,Aoki W,Ueda M,Faussner A,Rapala-Kozik M

    更新日期:2015-03-04 00:00:00

  • Characterization and regulation of the resistance-nodulation-cell division-type multidrug efflux pumps MdtABC and MdtUVW from the fire blight pathogen Erwinia amylovora.

    abstract:BACKGROUND:The Gram-negative bacterium Erwinia amylovora is the causal agent of the devastating disease fire blight in rosaceous plants such as apple, pear, quince, raspberry, and cotoneaster. In order to survive and multiply in a host, microbes must be able to circumvent the toxic effects of antimicrobial plant compou...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-14-185

    authors: Pletzer D,Weingart H

    更新日期:2014-07-11 00:00:00

  • Outer membrane protein P4 is not required for virulence in the human challenge model of Haemophilus ducreyi infection.

    abstract:BACKGROUND:Bacterial lipoproteins often play important roles in pathogenesis and can stimulate protective immune responses. Such lipoproteins are viable vaccine candidates. Haemophilus ducreyi, which causes the sexually transmitted disease chancroid, expresses a number of lipoproteins during human infection. One such l...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-14-166

    authors: Janowicz DM,Zwickl BW,Fortney KR,Katz BP,Bauer ME

    更新日期:2014-06-24 00:00:00

  • Microbial contamination and chemical toxicity of the Rio Grande.

    abstract:BACKGROUND:The Rio Grande River is the natural boundary between U.S. and Mexico from El Paso, TX to Brownsville, TX. and is one of the major water resources of the area. Agriculture, farming, maquiladora industry, domestic activities, as well as differences in disposal regulations and enforcement increase the contamina...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-4-17

    authors: Mendoza J,Botsford J,Hernandez J,Montoya A,Saenz R,Valles A,Vazquez A,Alvarez M

    更新日期:2004-04-22 00:00:00

  • A novel PBP3 substitution in Haemophilus influenzae confers reduced aminopenicillin susceptibility.

    abstract:BACKGROUND:Identification and characterization of non-typeable Haemophilus influenzae (NTHi) with reduced susceptibility to β-lactam antibiotics due to mutations in penicillin binding protein 3 (PBP3) is a clinical challenge. We analyzed a blood isolate, NTHi93-57485, that was categorized as aminopenicillin resistant b...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1196-6

    authors: Thegerström J,Matuschek E,Su YC,Riesbeck K,Resman F

    更新日期:2018-05-31 00:00:00

  • Phylogeographic evidence of cognate recognition site patterns and transformation efficiency differences in H. pylori: theory of strain dominance.

    abstract:BACKGROUND:Helicobacter pylori has diverged in parallel to its human host, leading to distinct phylogeographic populations. Recent evidence suggests that in the current human mixing in Latin America, European H. pylori (hpEurope) are increasingly dominant at the expense of Amerindian haplotypes (hspAmerind). This pheno...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-211

    authors: Maldonado-Contreras A,Mane SP,Zhang XS,Pericchi L,Alarcón T,Contreras M,Linz B,Blaser MJ,Domínguez-Bello MG

    更新日期:2013-09-19 00:00:00

  • CtcS, a MarR family regulator, regulates chlortetracycline biosynthesis.

    abstract:BACKGROUND:Chlortetracycline (CTC) is one of the commercially important tetracyclines (TCs) family product and is mainly produced by Streptomyces. CTC is still in a great demand due to its broad-spectrum activity against pathogens. Engineering transcriptional control allows the cell to allocate its valuable resources t...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1670-9

    authors: Kong L,Liu J,Zheng X,Deng Z,You D

    更新日期:2019-12-10 00:00:00

  • Genomic characterization of plant cell wall degrading enzymes and in silico analysis of xylanases and polygalacturonases of Fusarium virguliforme.

    abstract:BACKGROUND:Plant cell wall degrading enzymes (PCWDEs) are a subset of carbohydrate-active enzymes (CAZy) produced by plant pathogens to degrade plant cell walls. To counteract PCWDEs, plants release PCWDEs inhibitor proteins (PIPs) to reduce their impact. Several transgenic plants expressing exogenous PIPs that interac...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0761-0

    authors: Chang HX,Yendrek CR,Caetano-Anolles G,Hartman GL

    更新日期:2016-07-12 00:00:00

  • The process-related dynamics of microbial community during a simulated fermentation of Chinese strong-flavored liquor.

    abstract:BACKGROUND:Famous Chinese strong-flavored liquor (CSFL) is brewed by microbial consortia in a special fermentation pit (FT). However, the fermentation process was not fully understood owing to the complicate community structure and metabolism. In this study, the process-related dynamics of microbial communities and mai...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-017-1106-3

    authors: Zhang Y,Zhu X,Li X,Tao Y,Jia J,He X

    更新日期:2017-09-15 00:00:00

  • Extracellular DNA release confers heterogeneity in Candida albicans biofilm formation.

    abstract:BACKGROUND:Biofilm formation by Candida albicans has shown to be highly variable and is directly associated with pathogenicity and poor clinical outcomes in patients at risk. The aim of this study was to test the hypotheses that the extracellular DNA release by C. albicans is strain dependent and is associated with bio...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-014-0303-6

    authors: Rajendran R,Sherry L,Lappin DF,Nile CJ,Smith K,Williams C,Munro CA,Ramage G

    更新日期:2014-12-05 00:00:00

  • A metabolomics and proteomics study of the Lactobacillus plantarum in the grass carp fermentation.

    abstract:BACKGROUND:Lactobacillus plantarum, a versatile lactic acid-fermenting bacterium, isolated from the traditional pickles in Ningbo of China, was chosen for grass carp fermentation, which could also improve the flavor of grass carp. We here explored the central metabolic pathways of L. plantarum by using metabolomic appr...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1354-x

    authors: Ming T,Han J,Li Y,Lu C,Qiu D,Li Y,Zhou J,Su X

    更新日期:2018-12-18 00:00:00

  • Three redescriptions in Tintinnopsis (Protista: Ciliophora: Tintinnina) from coastal waters of China, with cytology and phylogenetic analyses based on ribosomal RNA genes.

    abstract:BACKGROUND:The taxonomy of tintinnine ciliates is vastly unresolved because it has traditionally been based on the lorica (a secreted shell) and it has only recently incorporated cytological and molecular information. Tintinnopsis, the most speciose tintinnine genus, is also the most problematic: it is known to be non-...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-02057-2

    authors: Bai Y,Wang R,Song W,Li L,Santoferrara LF,Hu X

    更新日期:2020-12-14 00:00:00

  • Genetic characterization of psp encoding the DING protein in Pseudomonas fluorescens SBW25.

    abstract:BACKGROUND:DING proteins constitute a conserved and broadly distributed set of proteins found in bacteria, fungi, plants and animals (including humans). Characterization of DING proteins from animal and plant tissues indicated ligand-binding ability suggesting a role for DING proteins in cell signaling and biomineraliz...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-7-114

    authors: Zhang XX,Scott K,Meffin R,Rainey PB

    更新日期:2007-12-18 00:00:00

  • Human milk fungi: environmental determinants and inter-kingdom associations with milk bacteria in the CHILD Cohort Study.

    abstract:BACKGROUND:Fungi constitute an important yet frequently neglected component of the human microbiota with a possible role in health and disease. Fungi and bacteria colonise the infant gastrointestinal tract in parallel, yet most infant microbiome studies have ignored fungi. Milk is a source of diverse and viable bacteri...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01829-0

    authors: Moossavi S,Fehr K,Derakhshani H,Sbihi H,Robertson B,Bode L,Brook J,Turvey SE,Moraes TJ,Becker AB,Mandhane PJ,Sears MR,Khafipour E,Subbarao P,Azad MB

    更新日期:2020-06-05 00:00:00

  • Helicobacter pylori HP0377, a member of the Dsb family, is an untypical multifunctional CcmG that cooperates with dimeric thioldisulfide oxidase HP0231.

    abstract:BACKGROUND:In the genome of H. pylori 26695, 149 proteins containing the CXXC motif characteristic of thioldisulfide oxidoreductases have been identified to date. However, only two of these proteins have a thioredoxin-like fold (i.e., HP0377 and HP0231) and are periplasm-located. We have previously shown that HP0231 is...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0471-z

    authors: Roszczenko P,Grzeszczuk M,Kobierecka P,Wywial E,Urbanowicz P,Wincek P,Nowak E,Jagusztyn-Krynicka EK

    更新日期:2015-07-04 00:00:00