Combined prime-boost vaccination against tick-borne encephalitis (TBE) using a recombinant vaccinia virus and a bacterial plasmid both expressing TBE virus non-structural NS1 protein.

Abstract:

BACKGROUND:Heterologous prime-boost immunization protocols using different gene expression systems have proven to be successful tools in protecting against various diseases in experimental animal models. The main reason for using this approach is to exploit the ability of expression cassettes to prime or boost the immune system in different ways during vaccination procedures. The purpose of the project was to study the ability of recombinant vaccinia virus (VV) and bacterial plasmid, both carrying the NS1 gene from tick-borne encephalitis (TBE) virus under the control of different promoters, to protect mice against lethal challenge using a heterologous prime-boost vaccination protocol. RESULTS:The heterologous prime-boost vaccination protocol, using a VV recombinant and bacterial plasmid, both containing the NS1 TBE virus protein gene under the control of different promoters, achieved a high level of protection in mice against lethal challenge with a highly pathogenic TBE virus strain. No signs of pronounced TBE infection were detected in the surviving animals. CONCLUSION:Heterologous prime-boost vaccination protocols using recombinant VV and bacterial plasmids could be used for the development of flavivirus vaccines.

journal_name

BMC Microbiol

journal_title

BMC microbiology

authors

Aleshin SE,Timofeev AV,Khoretonenko MV,Zakharova LG,Pashvykina GV,Stephenson JR,Shneider AM,Altstein AD

doi

10.1186/1471-2180-5-45

keywords:

subject

Has Abstract

pub_date

2005-08-02 00:00:00

pages

45

issn

1471-2180

pii

1471-2180-5-45

journal_volume

5

pub_type

杂志文章
  • Comparative analysis of diguanylate cyclase and phosphodiesterase genes in Klebsiella pneumoniae.

    abstract:BACKGROUND:Klebsiella pneumoniae can be found in environmental habitats as well as in hospital settings where it is commonly associated with nosocomial infections. One of the factors that contribute to virulence is its capacity to form biofilms on diverse biotic and abiotic surfaces. The second messenger Bis-(3'-5')-cy...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-12-139

    authors: Cruz DP,Huertas MG,Lozano M,Zárate L,Zambrano MM

    更新日期:2012-07-09 00:00:00

  • Characterization and anti-salmonella activities of lactic acid bacteria isolated from cattle faeces.

    abstract:BACKGROUND:Non typhoidal salmonellosis is one of the neglected zoonoses in most African countries. The use of sub-therapeutic doses of antibiotics as animal growth promoter enhances the emergence and dissemination of antimicrobial resistance in bacteria with food animal reservoirs and may also results in antibiotics re...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1248-y

    authors: Adetoye A,Pinloche E,Adeniyi BA,Ayeni FA

    更新日期:2018-08-30 00:00:00

  • Comparative diversity of microbiomes and Resistomes in beef feedlots, downstream environments and urban sewage influent.

    abstract:BACKGROUND:Comparative knowledge of microbiomes and resistomes across environmental interfaces between animal production systems and urban settings is lacking. In this study, we executed a comparative analysis of the microbiota and resistomes of metagenomes from cattle feces, catch basin water, manured agricultural soi...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1548-x

    authors: Zaheer R,Lakin SM,Polo RO,Cook SR,Larney FJ,Morley PS,Booker CW,Hannon SJ,Van Domselaar G,Read RR,McAllister TA

    更新日期:2019-08-27 00:00:00

  • Identification of DNA sequence variation in Campylobacter jejuni strains associated with the Guillain-Barré syndrome by high-throughput AFLP analysis.

    abstract:BACKGROUND:Campylobacter jejuni is the predominant cause of antecedent infection in post-infectious neuropathies such as the Guillain-Barré (GBS) and Miller Fisher syndromes (MFS). GBS and MFS are probably induced by molecular mimicry between human gangliosides and bacterial lipo-oligosaccharides (LOS). This study desc...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-6-32

    authors: Godschalk PC,Bergman MP,Gorkink RF,Simons G,van den Braak N,Lastovica AJ,Endtz HP,Verbrugh HA,van Belkum A

    更新日期:2006-04-04 00:00:00

  • Planctomycetes dominate biofilms on surfaces of the kelp Laminaria hyperborea.

    abstract:BACKGROUND:Bacteria belonging to Planctomycetes display several unique morphological and genetic features and are found in a wide variety of habitats on earth. Their ecological roles in these habitats are still poorly understood. Planctomycetes have previously been detected throughout the year on surfaces of the kelp L...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-261

    authors: Bengtsson MM,Øvreås L

    更新日期:2010-10-15 00:00:00

  • Robustness analysis of culturing perturbations on Escherichia coli colony biofilm beta-lactam and aminoglycoside antibiotic tolerance.

    abstract:BACKGROUND:Biofilms are ubiquitous. For instance, the majority of medical infections are thought to involve biofilms. However even after decades of investigation, the in vivo efficacy of many antimicrobial strategies is still debated suggesting there is a need for better understanding of biofilm antimicrobial tolerance...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-185

    authors: Zuroff TR,Bernstein H,Lloyd-Randolfi J,Jimenez-Taracido L,Stewart PS,Carlson RP

    更新日期:2010-07-07 00:00:00

  • Kinin release from human kininogen by 10 aspartic proteases produced by pathogenic yeast Candida albicans.

    abstract:BACKGROUND:Candida albicans yeast produces 10 distinct secreted aspartic proteases (Saps), which are some of the most important virulence factors of this pathogenic fungus. One of the suggested roles of Saps is their deregulating effect on various proteolytic cascades that constitute the major homeostatic systems in hu...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0394-8

    authors: Kozik A,Gogol M,Bochenska O,Karkowska-Kuleta J,Wolak N,Kamysz W,Aoki W,Ueda M,Faussner A,Rapala-Kozik M

    更新日期:2015-03-04 00:00:00

  • Analysis of and function predictions for previously conserved hypothetical or putative proteins in Blochmannia floridanus.

    abstract:BACKGROUND:There is an increasing interest to better understand endosymbiont capabilities in insects both from an ecological point of view and for pest control. Blochmannia floridanus provides important nutrients for its host, the ant Camponotus, while the bacterium in return is provided with a niche to proliferate. Bl...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-6-1

    authors: Gaudermann P,Vogl I,Zientz E,Silva FJ,Moya A,Gross R,Dandekar T

    更新日期:2006-01-09 00:00:00

  • Antimicrobial activity of copper surfaces against suspensions of Salmonella enterica and Campylobacter jejuni.

    abstract:BACKGROUND:Salmonella enterica and Campylobacter jejuni are amongst the more prevalent bacterial pathogens that cause foodborne diseases. These microorganisms are common contaminants of poultry and poultry products. This study was aimed to evaluate the antibacterial activity of metallic copper surfaces on these importa...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-4-19

    authors: Faúndez G,Troncoso M,Navarrete P,Figueroa G

    更新日期:2004-04-30 00:00:00

  • Three redescriptions in Tintinnopsis (Protista: Ciliophora: Tintinnina) from coastal waters of China, with cytology and phylogenetic analyses based on ribosomal RNA genes.

    abstract:BACKGROUND:The taxonomy of tintinnine ciliates is vastly unresolved because it has traditionally been based on the lorica (a secreted shell) and it has only recently incorporated cytological and molecular information. Tintinnopsis, the most speciose tintinnine genus, is also the most problematic: it is known to be non-...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-02057-2

    authors: Bai Y,Wang R,Song W,Li L,Santoferrara LF,Hu X

    更新日期:2020-12-14 00:00:00

  • Sequence analysis of percent G+C fraction libraries of human faecal bacterial DNA reveals a high number of Actinobacteria.

    abstract:BACKGROUND:The human gastrointestinal (GI) tract microbiota is characterised by an abundance of uncultured bacteria most often assigned in phyla Firmicutes and Bacteroidetes. Diversity of this microbiota, even though approached with culture independent techniques in several studies, still requires more elucidation. The...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-68

    authors: Krogius-Kurikka L,Kassinen A,Paulin L,Corander J,Mäkivuokko H,Tuimala J,Palva A

    更新日期:2009-04-08 00:00:00

  • Critical aspects of using bacterial cell viability assays with the fluorophores SYTO9 and propidium iodide.

    abstract:BACKGROUND:Viability staining with SYTO9 and propidium iodide (PI) is a frequently used tool in microbiological studies. However, data generated by such routinely used method are often not critically evaluated for their accuracy. In this study we aim to investigate the critical aspects of this staining method using Sta...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0376-x

    authors: Stiefel P,Schmidt-Emrich S,Maniura-Weber K,Ren Q

    更新日期:2015-02-18 00:00:00

  • Proposal of a quantitative PCR-based protocol for an optimal Pseudomonas aeruginosa detection in patients with cystic fibrosis.

    abstract:BACKGROUND:The lung of patients with cystic fibrosis (CF) is particularly sensitive to Pseudomonas aeruginosa. This bacterium plays an important role in the poor outcome of CF patients. During the disease progress, first acquisition of P. aeruginosa is the key-step in the management of CF patients. Quantitative PCR (qP...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-143

    authors: Le Gall F,Le Berre R,Rosec S,Hardy J,Gouriou S,Boisramé-Gastrin S,Vallet S,Rault G,Payan C,Héry-Arnaud G

    更新日期:2013-06-21 00:00:00

  • Did granny know best? Evaluating the antibacterial, antifungal and antiviral efficacy of acetic acid for home care procedures.

    abstract:BACKGROUND:Acetic acid has been used to clean and disinfect surfaces in the household for many decades. The antimicrobial efficacy of cleaning procedures can be considered particularly important for young, old, pregnant, immunocompromised people, but may also concern other groups, particularly with regards to the COVID...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01948-8

    authors: Zinn MK,Bockmühl D

    更新日期:2020-08-26 00:00:00

  • Influence of plant diversity and elevated atmospheric carbon dioxide levels on belowground bacterial diversity.

    abstract:BACKGROUND:Changes in aboveground plant species diversity as well as variations of environmental conditions such as exposure of ecosystems to elevated concentrations of atmospheric carbon dioxide may lead to changes in metabolic activity, composition and diversity of belowground microbial communities, both bacterial an...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-6-68

    authors: Grüter D,Schmid B,Brandl H

    更新日期:2006-07-27 00:00:00

  • Effects of disruption of heat shock genes on susceptibility of Escherichia coli to fluoroquinolones.

    abstract:BACKGROUND:It is well known that expression of certain bacterial genes responds rapidly to such stimuli as exposure to toxic chemicals and physical agents. It is generally believed that the proteins encoded in these genes are important for successful survival of the organism under the hostile conditions. Analogously, t...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-3-16

    authors: Yamaguchi Y,Tomoyasu T,Takaya A,Morioka M,Yamamoto T

    更新日期:2003-08-12 00:00:00

  • Portability of the thiolation domain in recombinant pyoverdine non-ribosomal peptide synthetases.

    abstract:BACKGROUND:Non-ribosomal peptide synthetase (NRPS) enzymes govern the assembly of amino acids and related monomers into peptide-like natural products. A key goal of the field is to develop methods to effective recombine NRPS domains or modules, and thereby generate modified or entirely novel products. We previously sho...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0496-3

    authors: Calcott MJ,Ackerley DF

    更新日期:2015-08-13 00:00:00

  • Evolutionary relationships of ATP-Binding Cassette (ABC) uptake porters.

    abstract:BACKGROUND:The ATP-Binding Cassette (ABC) functional superfamily includes integral transmembrane exporters that have evolved three times independently, forming three families termed ABC1, ABC2 and ABC3, upon which monophyletic ATPases have been superimposed for energy-coupling purposes [e.g., J Membr Biol 231(1):1-10, ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-98

    authors: Zheng WH,Västermark Å,Shlykov MA,Reddy V,Sun EI,Saier MH Jr

    更新日期:2013-05-06 00:00:00

  • Comparative gene expression analysis of planktonic Porphyromonas gingivalis ATCC 33277 in the presence of a growing biofilm versus planktonic cells.

    abstract:BACKGROUND:Porphyromonas gingivalis, a microorganism residing in the oral cavity within complex multispecies biofilms, is one of the keystone pathogens in the onset and progression of periodontitis. In this in vitro study, using DNA microarray, we investigate the differential gene expression of Porphyromonas gingivalis...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1423-9

    authors: Sánchez MC,Romero-Lastra P,Ribeiro-Vidal H,Llama-Palacios A,Figuero E,Herrera D,Sanz M

    更新日期:2019-03-12 00:00:00

  • CCL5 regulation of mucosal chlamydial immunity and infection.

    abstract:BACKGROUND:Following genital chlamydial infection, an early T helper type 1 (Th1)-associated immune response precedes the activation and recruitment of specific Th1 cells bearing distinct chemokine receptors, subsequently leading to the clearance of Chlamydia. We have shown that CCR5, a receptor for CCL5, is crucial fo...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-136

    authors: Sakthivel SK,Singh UP,Singh S,Taub DD,Igietseme JU,Lillard JW Jr

    更新日期:2008-08-13 00:00:00

  • In vitro synergy of sertraline and tetracycline cannot be reproduced in pigs orally challenged with a tetracycline resistant Escherichia coli.

    abstract:BACKGROUND:Antimicrobial helper-compounds may reverse antimicrobial resistance. Sertraline, a antidepressant drug, has been suggested as a tetracycline helper-compound. Tetracycline is the preferred antimicrobial for treatment of enteric diseases in pigs. This study is the first to evaluate the potency of sertraline as...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1383-5

    authors: Kromann S,Hvidtfeldt A,Boye M,Sørensen DB,Jørgensen S,Nielsen JP,Olsen RH

    更新日期:2019-01-11 00:00:00

  • Genetic stability of Brucella abortus isolates from an outbreak by multiple-locus variable-number tandem repeat analysis (MLVA16).

    abstract:BACKGROUND:Brucellosis caused by Brucella abortus is one of the most important zoonoses in the world. Multiple-locus variable-number tandem repeat analysis (MLVA16) has been shown be a useful tool to epidemiological traceback studies in B. abortus infection. Thus, the present study aimed (i) to evaluate the genetic div...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-14-186

    authors: Dorneles EM,Santana JA,Alves TM,Pauletti RB,Mol JP,Heinemann MB,Lage AP

    更新日期:2014-07-11 00:00:00

  • Genome-wide expression profiling of the response to short-term exposure to fluconazole in Cryptococcus neoformans serotype A.

    abstract:BACKGROUND:Fluconazole (FLC), a triazole antifungal drug, is widely used for the maintenance therapy of cryptococcal meningoencephalitis, the most common opportunistic infection in AIDS patients. In this study, we examined changes in the gene expression profile of the C. neoformans reference strain H99 (serotype A) fol...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-11-97

    authors: Florio AR,Ferrari S,De Carolis E,Torelli R,Fadda G,Sanguinetti M,Sanglard D,Posteraro B

    更新日期:2011-05-11 00:00:00

  • Emodin targets the beta-hydroxyacyl-acyl carrier protein dehydratase from Helicobacter pylori: enzymatic inhibition assay with crystal structural and thermodynamic characterization.

    abstract:BACKGROUND:The natural product Emodin demonstrates a wide range of pharmacological properties including anticancer, anti-inflammatory, antiproliferation, vasorelaxant and anti-H. pylori activities. Although its H. pylori inhibition was discovered, no acting target information against Emodin has been revealed to date. ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-91

    authors: Chen J,Zhang L,Zhang Y,Zhang H,Du J,Ding J,Guo Y,Jiang H,Shen X

    更新日期:2009-05-12 00:00:00

  • Temporal activation of anti- and pro-apoptotic factors in human gingival fibroblasts infected with the periodontal pathogen, Porphyromonas gingivalis: potential role of bacterial proteases in host signalling.

    abstract:BACKGROUND:Porphyromonas gingivalis is the foremost oral pathogen of adult periodontitis in humans. However, the mechanisms of bacterial invasion and the resultant destruction of the gingival tissue remain largely undefined. RESULTS:We report host-P. gingivalis interactions in primary human gingival fibroblast (HGF) c...

    journal_title:BMC microbiology

    pub_type: 杂志文章,收录出版

    doi:10.1186/1471-2180-6-26

    authors: Urnowey S,Ansai T,Bitko V,Nakayama K,Takehara T,Barik S

    更新日期:2006-03-08 00:00:00

  • Diversity and phylogenetic analysis of endosymbiotic bacteria of the date palm root borer Oryctes agamemnon (Coleoptera: Scarabaeidae).

    abstract:BACKGROUND:The date palm root borer Oryctes agamemnon (Coleoptera: Scarabaeidae) is one of the major pests of palms. In Saudi Arabia, both larvae and adults of Oryctes are particularly troublesome, especially during the establishment of young date palm orchards. Endosymbiotic bacteria are known to have a key role in fo...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0422-8

    authors: El-Sayed WS,Ibrahim RA

    更新日期:2015-04-22 00:00:00

  • Characterization and partial purification of Candida albicans Secretory IL-12 Inhibitory Factor.

    abstract:BACKGROUND:We have previously shown that supernatant from Candida albicans (CA) culture contains a Secretory Interleukin (IL)-12 Inhibitory Factor (CA-SIIF), which inhibits IL-12 production by human monocytes. However, the effect of CA-SIIF on secretion of other cytokines by monocytes is unknown, and detailed character...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-31

    authors: Wang M,Mukherjee PK,Chandra J,Lattif AA,McCormick TS,Ghannoum MA

    更新日期:2008-02-19 00:00:00

  • Evaluation of the interaction between polymyxin B and Pseudomonas aeruginosa biofilm and planktonic cells: reactive oxygen species induction and zeta potential.

    abstract:BACKGROUND:Although the most widely accepted mechanism of action for polymyxins is related to bacterial lysis via disruption, we hypothesized that this antimicrobial drug class could have other effects on Pseudomonas aeruginosa planktonic and sessile cells. Little is known regarding oxidative burst and zeta potential (...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1485-8

    authors: Lima MR,Ferreira GF,Nunes Neto WR,Monteiro JM,Santos ÁRC,Tavares PB,Denadai ÂML,Bomfim MRQ,Dos Santos VL,Marques SG,de Souza Monteiro A

    更新日期:2019-05-29 00:00:00

  • Special issue on enhancing vector refractoriness to trypanosome infection-foreword.

    abstract:: ...

    journal_title:BMC microbiology

    pub_type:

    doi:10.1186/s12866-018-1279-4

    authors: Abd-Alla AMM,Tsiamis G,Boucias DG

    更新日期:2018-11-23 00:00:00

  • Global regulation of gene expression in response to cysteine availability in Clostridium perfringens.

    abstract:BACKGROUND:Cysteine has a crucial role in cellular physiology and its synthesis is tightly controlled due to its reactivity. However, little is known about the sulfur metabolism and its regulation in clostridia compared with other firmicutes. In Clostridium perfringens, the two-component system, VirR/VirS, controls the...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-234

    authors: André G,Haudecoeur E,Monot M,Ohtani K,Shimizu T,Dupuy B,Martin-Verstraete I

    更新日期:2010-09-07 00:00:00