Abstract:
BACKGROUND:Campylobacter jejuni is the predominant cause of antecedent infection in post-infectious neuropathies such as the Guillain-Barré (GBS) and Miller Fisher syndromes (MFS). GBS and MFS are probably induced by molecular mimicry between human gangliosides and bacterial lipo-oligosaccharides (LOS). This study describes a new C. jejuni-specific high-throughput AFLP (htAFLP) approach for detection and identification of DNA polymorphism, in general, and of putative GBS/MFS-markers, in particular. RESULTS:We compared 6 different isolates of the "genome strain" NCTC 11168 obtained from different laboratories. HtAFLP analysis generated approximately 3000 markers per stain, 19 of which were polymorphic. The DNA polymorphisms could not be confirmed by PCR-RFLP analysis, suggesting a baseline level of 0.6% AFLP artefacts. Comparison of NCTC 11168 with 4 GBS-associated strains revealed 23 potentially GBS-specific markers, 17 of which were identified by DNA sequencing. A collection of 27 GBS/MFS-associated and 17 enteritis control strains was analyzed with PCR-RFLP tests based on 11 of these markers. We identified 3 markers, located in the LOS biosynthesis genes cj1136, cj1138 and cj1139c, that were significantly associated with GBS (P = 0.024, P = 0.047 and P < 0.001, respectively). HtAFLP analysis of 13 highly clonal South African GBS/MFS-associated and enteritis control strains did not reveal GBS-specific markers. CONCLUSION:This study shows that bacterial GBS markers are limited in number and located in the LOS biosynthesis genes, which corroborates the current consensus that LOS mimicry may be the prime etiologic determinant of GBS. Furthermore, our results demonstrate that htAFLP, with its high reproducibility and resolution, is an effective technique for the detection and subsequent identification of putative bacterial disease markers.
journal_name
BMC Microbioljournal_title
BMC microbiologyauthors
Godschalk PC,Bergman MP,Gorkink RF,Simons G,van den Braak N,Lastovica AJ,Endtz HP,Verbrugh HA,van Belkum Adoi
10.1186/1471-2180-6-32subject
Has Abstractpub_date
2006-04-04 00:00:00pages
32issn
1471-2180pii
1471-2180-6-32journal_volume
6pub_type
杂志文章相关文献
BMC MICROBIOLOGY文献大全abstract:BACKGROUND:Clostridium termitidis CT1112 is an anaerobic, Gram-positive, mesophilic, spore-forming, cellulolytic bacterium, originally isolated from the gut of a wood feeding termite Nasusitermes lujae. It has the ability to hydrolyze both cellulose and hemicellulose, and ferment the degradation products to acetate, fo...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0711-x
更新日期:2016-05-23 00:00:00
abstract:BACKGROUND:Mycobacterium avium is an environmental mycobacterium that can be divided into the subspecies avium, hominissuis, paratuberculosis and silvaticum. Some M. avium subspecies are opportunistic pathogens for animals and humans. They are ubiquitous in nature and can be isolated from natural sources of water, soil...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-7-14
更新日期:2007-03-05 00:00:00
abstract:BACKGROUND:Adhesion to surfaces facilitates many crucial functions of microbes in their natural habitats. Thus understanding the mechanism of microbial adhesion is of broad interest to the microbiology research community. RESULTS:We report a study by fluorescence imaging and atomic force microscopy on the growth in si...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-139
更新日期:2013-06-19 00:00:00
abstract:BACKGROUND:Legionella pneumophila, the causative agent of Legionnaire's disease, may enter a viable but non-culturable (VBNC) state triggered by environmental stress conditions. Specific outer-membrane epitopes of L. pneumophila are used in many diagnostic applications and some of them are linked to important virulence...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1220-x
更新日期:2018-07-17 00:00:00
abstract:BACKGROUND:Recurrent acute otitis media (rAOM, recurrent ear infection) is a common childhood disease caused by bacteria termed otopathogens, for which current treatments have limited effectiveness. Generic probiotic therapies have shown promise, but seem to lack specificity. We hypothesised that healthy children with ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1154-3
更新日期:2018-02-20 00:00:00
abstract:BACKGROUND:Mycoplasma pneumoniae has previously been characterized as a micro-organism that is genetically highly stable. In spite of this genetic stability, homologous DNA recombination has been hypothesized to lie at the basis of antigenic variation of the major surface protein, P1, of M. pneumoniae. In order to iden...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-167
更新日期:2008-10-02 00:00:00
abstract:BACKGROUND:Porphyromonas gingivalis is the foremost oral pathogen of adult periodontitis in humans. However, the mechanisms of bacterial invasion and the resultant destruction of the gingival tissue remain largely undefined. RESULTS:We report host-P. gingivalis interactions in primary human gingival fibroblast (HGF) c...
journal_title:BMC microbiology
pub_type: 杂志文章,收录出版
doi:10.1186/1471-2180-6-26
更新日期:2006-03-08 00:00:00
abstract:BACKGROUND:Microorganisms of different species interact in several ecological niches, even causing infection. During the infectious process, a biofilm of single or multispecies can develop. Aspergillus fumigatus and Staphyloccocus aureus are etiologic agents that can cause infectious keratitis. We analyzed in vitro sin...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0363-2
更新日期:2015-02-15 00:00:00
abstract:BACKGROUND:It is unclear how DNA is packaged in a bacterial cell in the absence of nucleosomes. To investigate the initial level of DNA condensation in bacterial nucleoid we used in vivo DNA digestion coupled with high-throughput sequencing of the digestion-resistant fragments. To this end, we transformed E. coli cells...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0637-3
更新日期:2016-02-20 00:00:00
abstract:BACKGROUND:ClpP is important for bacterial growth and plays an indispensable role in cellular protein quality control systems by refolding or degrading damaged proteins, but the physiological significance of ClpP in Enterococcus faecalis remains obscure. A clpP deletion mutant (△clpP) was constructed using the E. faeca...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-1719-9
更新日期:2020-02-07 00:00:00
abstract:BACKGROUND:The phenomenon of misdiagnosing tuberculosis (TB) by laboratory cross-contamination when culturing Mycobacterium tuberculosis (MTB) has been widely reported and it has an obvious clinical, therapeutic and social impact. The final confirmation of a cross-contamination event requires the molecular identificati...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-30
更新日期:2008-02-14 00:00:00
abstract:BACKGROUND:Pyruvate oxidase (Pox) is an important enzyme in bacterial metabolism for increasing ATP production and providing a fitness advantage via hydrogen peroxide production. However, few Pox enzymes have been characterized from bacterial species. The tetrameric non-hydrogen-peroxide producing Pox from E. coli is a...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01788-6
更新日期:2020-05-24 00:00:00
abstract:BACKGROUND:Wolbachia, one of the most abundant taxa of intracellular Alphaproteobacteria, is widespread among arthropods and filarial nematodes. The presence of these maternally inherited bacteria is associated with modifications of host fitness, including a variety of reproductive abnormalities, such as cytoplasmic in...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1652-y
更新日期:2019-12-24 00:00:00
abstract:BACKGROUND:HSV-1 is a common pathogen that infects 50-90% of the human population worldwide. HSV-1 causes numerous infection-related diseases, some of which are severely life-threatening. There are antiviral medications with activity against HSV-1. However, with the emergence of drug-resistant mutant strains of HSV-1, ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1639-8
更新日期:2019-12-07 00:00:00
abstract:BACKGROUND:Most of the studies evaluating the secular trends of blood isolates come from tertiary hospitals in urban areas. We sought to study the trends of the antimicrobial resistance of blood isolates in patients from a rural population hospitalized in a tertiary hospital in a small city in Greece. METHODS:We retro...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-6-41
更新日期:2006-05-02 00:00:00
abstract:BACKGROUND:Enterohemorrhagic Escherichia coli (EHEC) colonizes the intestinal epithelium and causes attaching and effacing (A/E) lesions. Expression of virulence genes, particularly those from the locus of the enterocyte effacement (LEE) pathogenicity island is required for the formation of a type three secretion syste...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-231
更新日期:2012-10-11 00:00:00
abstract:BACKGROUND:Photorhabdus are Gram-negative nematode-symbiotic and insect-pathogenic bacteria. The species Photorhabdus asymbiotica is able to infect humans as well as insects. We investigated the secreted proteome of a clinical isolate of P. asymbiotica at different temperatures in order to identify proteins relevant to...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-141
更新日期:2010-05-12 00:00:00
abstract:BACKGROUND:Chlortetracycline (CTC) is one of the commercially important tetracyclines (TCs) family product and is mainly produced by Streptomyces. CTC is still in a great demand due to its broad-spectrum activity against pathogens. Engineering transcriptional control allows the cell to allocate its valuable resources t...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1670-9
更新日期:2019-12-10 00:00:00
abstract:BACKGROUND:The plasma membrane plays an essential role in selective permeability, compartmentalization, osmotic balance, and cellular uptake. The characteristics and functions of cyanobacterial membranes have been extensively investigated in recent years. Cell-penetrating peptides (CPPs) are special nanocarriers that c...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-57
更新日期:2013-03-14 00:00:00
abstract:BACKGROUND:A common assumption of microorganisms is that laboratory stocks will remain genetically and phenotypically constant over time, and across laboratories. It is becoming increasingly clear, however, that mutations can ruin strain integrity and drive the divergence or "domestication" of stocks. Since its discove...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-14-2
更新日期:2014-01-02 00:00:00
abstract:BACKGROUND:Successful viral infection requires the involvement of host cellular factors in their life cycle. Heat shock protein 70 (HSP70) can be recruited by numerous viruses to promote the folding, maturation, or assembly of viral proteins. We have previously shown that HSP70 is significantly elevated in porcine repr...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-14-64
更新日期:2014-03-13 00:00:00
abstract:BACKGROUND:Lyme borrelia genotypes differ in their capacity to cause disseminated disease. Gene array analysis was employed to profile the host transcriptome induced by Borrelia burgdorferi strains with different capacities for causing disseminated disease in the blood of C3H/HeJ mice during early infection. RESULTS:B...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0644-4
更新日期:2016-03-08 00:00:00
abstract:BACKGROUND:The high demand for ethanol in the U.S. has generated large stocks of wet distillers grains (DG), a byproduct from the manufacture of ethanol from corn and sorghum grains. Little is known, however, about the potential influence of dietary DG on fecal microbial community structure. A better understanding of t...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-25
更新日期:2012-02-24 00:00:00
abstract:BACKGROUND:Several intracellular bacterial pathogens have evolved subtle strategies to subvert vesicular trafficking pathways of their host cells to avoid killing and to replicate inside the cells. Brucellae are Gram-negative facultative intracellular bacteria that are responsible for brucellosis, a worldwide extended ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-014-0223-5
更新日期:2014-09-02 00:00:00
abstract:BACKGROUND:The heme acquisition machinery in Streptococcus pyogenes is believed to consist of the surface proteins, Shr and Shp, and heme-specific ATP-binding cassette transporter HtsABC. Shp has been shown to rapidly transfer its heme to the lipoprotein component, HtsA, of HtsABC. The function of Shr and the heme sour...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-15
更新日期:2008-01-23 00:00:00
abstract:BACKGROUND:The emergence of carbapenemase producing bacteria, especially New Delhi metallo-β-lactamase (NDM-1) and its variants, worldwide, has raised amajor public health concern. NDM-1 hydrolyzes a wide range of β-lactam antibiotics, including carbapenems, which are the last resort of antibiotics for the treatment of...
journal_title:BMC microbiology
pub_type: 杂志文章,评审
doi:10.1186/s12866-017-1012-8
更新日期:2017-04-27 00:00:00
abstract:BACKGROUND:Affecting the core functional microbiome, peculiar high level taxonomic unbalances of the human intestinal microbiota have been recently associated with specific diseases, such as obesity, inflammatory bowel diseases, and intestinal inflammation. RESULTS:In order to specifically monitor microbiota unbalance...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-116
更新日期:2010-04-19 00:00:00
abstract:BACKGROUND:Sigma factors are one of the components of RNA polymerase holoenzymes, and an essential factor of transcription initiation in bacteria. Corynebacterium glutamicum possesses seven genes coding for sigma factors, most of which have been studied to some detail; however, the role of SigD in transcriptional regul...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-1067-6
更新日期:2017-07-12 00:00:00
abstract:BACKGROUND:The flame-oxidized stainless steel anode (FO-SSA) is a newly developed electrode that enhances microbial fuel cell (MFC) power generation; however, substrate preference and community structure of the biofilm developed on FO-SSA have not been well characterized. Herein, we investigated the community on FO-SSA...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-1053-z
更新日期:2017-06-29 00:00:00
abstract:BACKGROUND:There is a rapid emergence of multiple resistant gram-negative bacteria due to overuse of antibiotics in the treatment of infections. Biofilms consist of polymicrobial communities that survive the host's defense system. The key bacteria in biofilms are slow growing and support an attachment and rapid growth ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0821-5
更新日期:2016-09-09 00:00:00