Abstract:
BACKGROUND:The human gastrointestinal (GI) tract microbiota is characterised by an abundance of uncultured bacteria most often assigned in phyla Firmicutes and Bacteroidetes. Diversity of this microbiota, even though approached with culture independent techniques in several studies, still requires more elucidation. The main purpose of this work was to study whether the genomic percent guanine and cytosine (%G+C) -based profiling and fractioning prior to 16S rRNA gene sequence analysis reveal higher microbiota diversity, especially with high G+C bacteria suggested to be underrepresented in previous studies. RESULTS:A phylogenetic analysis of the composition of the human GI microbiota of 23 healthy adult subjects was performed from a pooled faecal bacterial DNA sample by combining genomic %G+C -based profiling and fractioning with 16S rRNA gene cloning and sequencing. A total of 3199 partial 16S rRNA genes were sequenced. For comparison, 459 clones were sequenced from a comparable unfractioned sample. The most important finding was that the proportional amount of sequences affiliating with the phylum Actinobacteria was 26.6% in the %G+C fractioned sample but only 3.5% in the unfractioned sample. The orders Coriobacteriales, Bifidobacteriales and Actinomycetales constituted the 65 actinobacterial phylotypes in the fractioned sample, accounting for 50%, 47% and 3% of sequences within the phylum, respectively. CONCLUSION:This study shows that the %G+C profiling and fractioning prior to cloning and sequencing can reveal a significantly larger proportion of high G+C content bacteria within the clones recovered, compared with the unfractioned sample in the human GI tract. Especially the order Coriobacteriales within the phylum Actinobacteria was found to be more abundant than previously estimated with conventional sequencing studies.
journal_name
BMC Microbioljournal_title
BMC microbiologyauthors
Krogius-Kurikka L,Kassinen A,Paulin L,Corander J,Mäkivuokko H,Tuimala J,Palva Adoi
10.1186/1471-2180-9-68subject
Has Abstractpub_date
2009-04-08 00:00:00pages
68issn
1471-2180pii
1471-2180-9-68journal_volume
9pub_type
杂志文章相关文献
BMC MICROBIOLOGY文献大全abstract:BACKGROUND:The life cycle of a bacteriophage has tightly programmed steps to help virus infect its host through the interactions between the bacteriophage and its host proteins. However, bacteriophage-host protein interactions in high temperature environment remain poorly understood. To address this issue, the protein ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-48
更新日期:2013-02-26 00:00:00
abstract:BACKGROUND:Campylobacter jejuni (C. jejuni) is a leading cause of foodborne gastroenteritis worldwide. This bacterium lacks many of the classical virulence factors, and flagellum-associated persistent colonization has been shown to be crucial for its pathogenesis. The flagellum plays a multifunctional role in C. jejuni...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1318-1
更新日期:2018-10-22 00:00:00
abstract:BACKGROUND:Bacteriocins are antimicrobial proteins and peptides ribosomally synthesized by some bacteria which can effect both intraspecies and interspecies killing. RESULTS:Moraxella catarrhalis strain E22 containing plasmid pLQ510 was shown to inhibit the growth of M. catarrhalis strain O35E. Two genes (mcbA and mcb...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-207
更新日期:2009-09-25 00:00:00
abstract:BACKGROUND:After E. coli O157, E. coli O26 is the second most prevalent enterohaemorrhagic E. coli (EHEC) serotype identified in cases of foodborne illness in Australia and throughout the world. E. coli O26 associated foodborne outbreaks have drawn attention to the survival capabilities of this organism in a range of e...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-0963-0
更新日期:2017-03-01 00:00:00
abstract:BACKGROUND:Enterococci, ubiquitous colonizers of humans and other animals, play an increasingly important role in health-care associated infections (HAIs). It is believed that the recent evolution of two clinically relevant species, Enterococcus faecalis and Enterococcus faecium occurred in a big part in a hospital env...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-14-66
更新日期:2014-03-14 00:00:00
abstract:BACKGROUND:Streptococcus agalactiae or Group B Streptococci (GBS) have the ability to access various host sites, which reflects its adaptability to different environments during the course of infection. This adaptation is due to the expression of virulence factors that are involved with survival, invasion and bacterial...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-297
更新日期:2013-12-21 00:00:00
abstract:BACKGROUND:Despite the high prevalence of genotype 1b hepatitis C virus (HCV) among patients, a cell culture system that permits entire viral life cycle of genotype 1b isolates is limited. To develop a cell-cultured hepatitis C virus (HCVcc) of genotype 1b, the proper combination of HCV genomic variants and host cells ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0846-9
更新日期:2016-09-27 00:00:00
abstract:BACKGROUND:Most lactic acid bacteria are non-motile but some of them are flagellated and exhibit motility. So far, motile lactobacilli have rarely been studied, and characteristics of their flagellins are poorly understood. In this study, a highly motile strain of Lactobacillus agilis was recruited for transcriptional ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0667-x
更新日期:2016-03-22 00:00:00
abstract:BACKGROUND:Vibrio cholerae is the causative agent of cholera. Extensive studies reveal that complicated regulatory cascades regulate expression of virulence genes, the products of which are required for V. cholerae to colonize and cause disease. In this study, we investigated the expression of the key virulence regulat...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-3
更新日期:2010-01-06 00:00:00
abstract:BACKGROUND:Persister cells comprise a phenotypic variant that shows extreme antibiotic tolerance resulting in treatment failures of bacterial infections. While this phenomenon has posed a great threat in public health, mechanisms underlying their formation in Staphylococcus aureus remain largely unknown. Increasing evi...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-1129-9
更新日期:2017-11-22 00:00:00
abstract:BACKGROUND:Bacillus mycoides Flügge, a Gram-positive, non-motile soil bacterium assigned to Bacillus cereus group, grows on agar as chains of cells linked end to end, forming radial filaments curving clock- or counter-clockwise (SIN or DX morphotypes). The molecular mechanism causing asymmetric curving is not known: ou...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-2-33
更新日期:2002-11-13 00:00:00
abstract:BACKGROUND:Streptococcus pseudopneumoniae, is a novel member of the genus Streptococcus, falling close to related members like S. pneumoniae, S. mitis, and S. oralis. Its recent appearance has shed light on streptococcal infections, which has been unclear till recently. In this study, the transcriptome of S. pseudopneu...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-77
更新日期:2012-07-06 00:00:00
abstract:BACKGROUND:Fusobacterium nucleatum, a well studied bacterium in periodontal diseases, appendicitis, gingivitis, osteomyelitis and pregnancy complications has recently gained attention due to its association with colorectal cancer (CRC) progression. Treatment with berberine was shown to reverse F. nucleatum-induced CRC ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0700-0
更新日期:2016-05-12 00:00:00
abstract:BACKGROUND:The low bacterial load in samples acquired from the lungs, have made studies on the airway microbiome vulnerable to contamination from bacterial DNA introduced during sampling and laboratory processing. We have examined the impact of laboratory contamination on samples collected from the lower airways by pro...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1560-1
更新日期:2019-08-14 00:00:00
abstract:BACKGROUND:The global spread of bacterial resistance has given rise to a growing interest in new anti-bacterial agents with a new strategy of action. Pilicides are derivatives of ring-fused 2-pyridones which block the formation of the pili/fimbriae crucial to bacterial pathogenesis. They impair by means of a chaperone-...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-131
更新日期:2013-06-12 00:00:00
abstract:BACKGROUND:Candida albicans yeast produces 10 distinct secreted aspartic proteases (Saps), which are some of the most important virulence factors of this pathogenic fungus. One of the suggested roles of Saps is their deregulating effect on various proteolytic cascades that constitute the major homeostatic systems in hu...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0394-8
更新日期:2015-03-04 00:00:00
abstract:BACKGROUND:The balanced synthesis of membrane phospholipids, fatty acids and cell wall constituents is a vital facet of bacterial physiology, but there is little known about the biochemical control points that coordinate these activities in Gram-positive bacteria. In Escherichia coli, the glycerol-phosphate acyltransfe...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-260
更新日期:2013-11-16 00:00:00
abstract:BACKGROUND:Antimicrobial resistance is currently a major global issue. As the rate of emergence of antimicrobial resistance has superseded the rate of discovery and introduction of new effective drugs, the medical arsenal now is experiencing shortage of effective drugs to combat diseases, particularly against diseases ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-0936-3
更新日期:2017-02-16 00:00:00
abstract:BACKGROUND:The evolution of mycoplasmas from a common ancestor with Firmicutes has been characterized not only by genome down-sizing but also by horizontal gene transfer between mycoplasma species sharing a common host. The mechanisms of these gene transfers remain unclear because our knowledge of the mycoplasma mobile...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-257
更新日期:2012-11-12 00:00:00
abstract:BACKGROUND:Iron plays important roles in the growth, reproduction and pathogenicity of Aeromonas hydrophila. In this study, we detected and compared the mRNA and protein expression profiles of A. hydrophila under normal and iron restricted medium with 200 μM 2,2-Dipyridyl using RNA Sequencing (RNA-seq) and isobaric tag...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1178-8
更新日期:2018-06-04 00:00:00
abstract:BACKGROUND:In this study, we have evaluated the immunological status of hepatitis C virus (HCV)-infected patients aiming at identifying putative biomarkers associated with distinct degrees of liver fibrosis. Peripheral blood and tissue T-cells as well as cytokine levels were quantified by flow cytometry. RESULTS:Data ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0610-6
更新日期:2016-01-07 00:00:00
abstract:BACKGROUND:The Beijing lineage of Mycobacterium tuberculosis is causing concern due to its global distribution and its involvement in severe outbreaks. Studies focused on this lineage are mainly restricted to geographical settings where its prevalence is high, whereas those in other areas are scarce. In this study, we ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-151
更新日期:2010-05-25 00:00:00
abstract:BACKGROUND:Demand for high-throughput bioprocessing has dramatically increased especially in the biopharmaceutical industry because the technologies are of vital importance to process optimization and media development. This can be efficiently boosted by using microtiter plate (MTP) cultivation setup embedded into an a...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-1145-9
更新日期:2018-01-04 00:00:00
abstract:BACKGROUND:Neisseria gonorrhoeae is an obligate human pathogen that causes significant worldwide morbidity. N. gonorrhoeae expresses lipooligosaccharide (LOS), a phase variable molecule that plays an important role during pathogenesis of the organism. Alteration in the structure of gonococcal LOS correlates with altere...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-7-7
更新日期:2007-01-26 00:00:00
abstract:BACKGROUND:Mob family proteins are conserved between animals, plants and fungi and are essential for the activation of NDR kinases that control crucial cellular processes like cytokinesis, proliferation and morphology. RESULTS:We identified a hypomorphic allele of ChMOB2 in a random insertional mutant (vir-88) of the ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-0932-7
更新日期:2017-01-19 00:00:00
abstract:BACKGROUND:Trichomonas vaginalis is a human urogenital pathogen responsible for trichomonosis, the number-one, non-viral sexually transmitted disease (STD) worldwide, while T. tenax is a commensal of the human oral cavity, found particularly in patients with poor oral hygiene and advanced periodontal disease. The exten...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-58
更新日期:2009-03-18 00:00:00
abstract:BACKGROUND:Salmonella enteritidis is suggested to translocate in the small intestine. In vivo it induces gene expression changes in the ileal mucosa and Peyer's patches. Stimulation of Salmonella translocation by dietary prebiotics fermented in colon suggests involvement of the colon as well. However, effects of Salmon...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-7-84
更新日期:2007-09-12 00:00:00
abstract:BACKGROUND:Members of the genus Bifidobacterium are anaerobic Gram-positive Actinobacteria, which are natural inhabitants of human and animal gastrointestinal tract. Certain bifidobacteria are frequently used as food additives and probiotic pharmaceuticals, because of their various health-promoting properties. Due to t...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0779-3
更新日期:2016-07-22 00:00:00
abstract:BACKGROUND:Macrolide antibiotics are commonly administered for bacterial respiratory illnesses. Azithromycin (Az) is especially noted for extremely high intracellular concentrations achieved within macrophages which is far greater than the serum concentration. Clinical strains of Type B Francisella (F.) tularensis have...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-123
更新日期:2010-04-23 00:00:00
abstract:BACKGROUND:The ability of an intracellular pathogen to establish infection depends on the capacity of the organism to survive and replicate inside the host. Mycobacterium fortuitum is a bacteria that contains genes involved in the detoxification of the oxygen reactive species such as those produced by the host during t...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-100
更新日期:2008-06-19 00:00:00