Abstract:
BACKGROUND:Iron plays important roles in the growth, reproduction and pathogenicity of Aeromonas hydrophila. In this study, we detected and compared the mRNA and protein expression profiles of A. hydrophila under normal and iron restricted medium with 200 μM 2,2-Dipyridyl using RNA Sequencing (RNA-seq) and isobaric tags for relative and absolute quantification (iTRAQ) analyses. RESULTS:There were 1204 genes (601 up- and 603 down-regulated) and 236 proteins (90 up- and 146 down-regulated) shown to be differentially expressed, and 167 genes and proteins that showed consistent expression. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that the differentially expressed genes and proteins were mainly involved in iron ion transport, protein activity, energy metabolism and virulence processes. Further validation of the RNA-seq and iTRAQ results by quantitative real-time PCR (qPCR) revealed that 18 of the 20 selected genes were consistently expressed. The iron-ion absorption and concentration of A. hydrophila under iron-limited conditions were enhanced, and most virulence factors (protease activity, hemolytic activity, lipase activity, and swimming ability) were also increased. Artificial A. hydrophila infection caused higher mortality in cyprinid Megalobrama amblycephala under iron-limited conditions. CONCLUSION:Understanding the responses of pathogenic Aeromonas hydrophila within the hostile environment of the fish host, devoid of free iron, is important to reveal bacterial infection and pathogenesis. This study further confirmed the previous finding that iron-limitation efficiently enhanced the virulence of A. hydrophila using multi-omics analyses. We identified differentially expressed genes and proteins, related to enterobactin synthesis and virulence establishment, that play important roles in addressing iron scarcity.
journal_name
BMC Microbioljournal_title
BMC microbiologyauthors
Teng T,Xi B,Chen K,Pan L,Xie J,Xu Pdoi
10.1186/s12866-018-1178-8subject
Has Abstractpub_date
2018-06-04 00:00:00pages
52issue
1issn
1471-2180pii
10.1186/s12866-018-1178-8journal_volume
18pub_type
杂志文章相关文献
BMC MICROBIOLOGY文献大全abstract:BACKGROUND:Leptospires lack many of the homologs for oxidative defense present in other bacteria, but do encode homologs of the Bacteriodes aerotolerance (Bat) proteins, which have been proposed to fulfill this function. Bat homologs have been identified in all families of the phylum Spirochaetes, yet a specific functi...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-290
更新日期:2012-12-13 00:00:00
abstract:BACKGROUND:Pectobacterium carotovorum subsp. carotovorum is a phytopathogenic enterobacterium responsible for soft rot, a disease characterized by extensive maceration of the affected plant tissue. This species also produces two or more antibacterial substances called bacteriocins, which enhance its competitiveness aga...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-181
更新日期:2009-08-27 00:00:00
abstract:BACKGROUND:Chlamydiae are obligate intracellular pathogens that possess a type III secretion system to deliver proteins into the host cell during infection. Small molecule inhibitors of type III secretion in Yersinia, termed INPs (Innate Pharmaceuticals AB) were reported to strongly inhibit Chlamydia growth in epitheli...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-75
更新日期:2009-04-21 00:00:00
abstract:BACKGROUND:Alleviating arsenic (As) contamination is a high-priority environmental issue. Hyperaccumulator plants may harbor endophytic bacteria able to detoxify As. Therefore, we investigated the distribution, diversity, As (III) resistance levels, and resistance-related functional genes of arsenite-resistant bacteria...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1184-x
更新日期:2018-05-08 00:00:00
abstract:BACKGROUND:The microbiological diagnosis of bacterial vaginosis is usually made using Nugent's criteria, a useful but rather laborious scoring system based on counting bacterial cell types on Gram stained slides of vaginal smears. Ison and Hay have simplified the score system to three categories and added a fourth cate...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-5-61
更新日期:2005-10-14 00:00:00
abstract:BACKGROUND:The use of chemical fungicides against fungal pathogens adversely affects soil and plant health thereby resulting in overall environmental hazards. Therefore, biological source for obtaining antifungal agents is considered as an environment-friendly alternative for controlling fungal pathogens. RESULTS:In t...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1440-8
更新日期:2019-04-02 00:00:00
abstract:BACKGROUND:Staphylococcus aureus methicillin-resistant (MRSA) has been frequently isolated from endotracheal and lung puncture aspirates in malnourished children with pneumonia. In this work we evaluated the susceptibility of undernourished BALB/c mice and its ability to mount a protective immunity against MRSA with em...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-240
更新日期:2009-11-23 00:00:00
abstract:BACKGROUND:Campylobacter jejuni (C. jejuni) is a leading cause of foodborne gastroenteritis worldwide. This bacterium lacks many of the classical virulence factors, and flagellum-associated persistent colonization has been shown to be crucial for its pathogenesis. The flagellum plays a multifunctional role in C. jejuni...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1318-1
更新日期:2018-10-22 00:00:00
abstract:BACKGROUND:The gene encoding the inorganic pyrophosphatase (PPase) of the intracellular pathogen Legionella pneumophila is induced during intracellular infection, but is constitutively expressed in Escherichia coli. The causative agent of tuberculosis, Mycobacterium tuberculosis, contains a well conserved copy of PPase...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-1-3
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Persister cells comprise a phenotypic variant that shows extreme antibiotic tolerance resulting in treatment failures of bacterial infections. While this phenomenon has posed a great threat in public health, mechanisms underlying their formation in Staphylococcus aureus remain largely unknown. Increasing evi...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-1129-9
更新日期:2017-11-22 00:00:00
abstract:BACKGROUND:Tsetse flies (Diptera: Glossinidae) are solely responsible for the transmission of African trypanosomes, causative agents of sleeping sickness in humans and nagana in livestock. Due to the lack of efficient vaccines and the emergence of drug resistance, vector control approaches such as the sterile insect te...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1295-4
更新日期:2018-11-23 00:00:00
abstract:BACKGROUND:We have previously shown that supernatant from Candida albicans (CA) culture contains a Secretory Interleukin (IL)-12 Inhibitory Factor (CA-SIIF), which inhibits IL-12 production by human monocytes. However, the effect of CA-SIIF on secretion of other cytokines by monocytes is unknown, and detailed character...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-31
更新日期:2008-02-19 00:00:00
abstract:BACKGROUND:Successful viral infection requires the involvement of host cellular factors in their life cycle. Heat shock protein 70 (HSP70) can be recruited by numerous viruses to promote the folding, maturation, or assembly of viral proteins. We have previously shown that HSP70 is significantly elevated in porcine repr...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-14-64
更新日期:2014-03-13 00:00:00
abstract:BACKGROUND:Several conventional methods, including fungal culture and periodic acid-Schiff (PAS) reagent staining, have been used to diagnose oral candidiasis. The aim of this study was to evaluate the efficacy of a novel method, fungal fluorescent staining, in relation to conventional protocols in the diagnosis of ora...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1467-x
更新日期:2019-05-14 00:00:00
abstract:BACKGROUND:The ATP-Binding Cassette (ABC) functional superfamily includes integral transmembrane exporters that have evolved three times independently, forming three families termed ABC1, ABC2 and ABC3, upon which monophyletic ATPases have been superimposed for energy-coupling purposes [e.g., J Membr Biol 231(1):1-10, ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-98
更新日期:2013-05-06 00:00:00
abstract:BACKGROUND:The life cycle of a bacteriophage has tightly programmed steps to help virus infect its host through the interactions between the bacteriophage and its host proteins. However, bacteriophage-host protein interactions in high temperature environment remain poorly understood. To address this issue, the protein ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-48
更新日期:2013-02-26 00:00:00
abstract:BACKGROUND:Biofilm is a fundamental bacterial survival mode which proceeds through three main generalized phases: adhesion, maturation, and dispersion. Lactobacilli spp. (LB) are critical components of gut and reproductive health and are widely used probiotics. Evaluation of time-dependent mechanisms of biofilm formati...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01753-3
更新日期:2020-03-24 00:00:00
abstract:BACKGROUND:The catabolite control protein CcpA is a transcriptional regulator conserved in many Gram-positives, controlling the efficiency of glucose metabolism. Here we studied the role of Bacillus cereus ATCC 14579 CcpA in regulation of metabolic pathways and expression of enterotoxin genes by comparative transcripto...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-62
更新日期:2008-04-16 00:00:00
abstract:BACKGROUND:Lactobacillus species are used as probiotics and play an important role in fermented food production. However, use of 16S rRNA gene sequences as standard markers for the differentiation of Lactobacillus species offers a very limited scope, as several species of Lactobacillus share similar 16S rRNA gene seque...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01781-z
更新日期:2020-04-15 00:00:00
abstract:BACKGROUND:Tetrahymena thermophila is one of the best characterized unicellular eukaryotes and its genome is sequenced in its entirety. However, the AT-richness of the genome and an unusual codon usage cause problems in cloning and expression of the ciliate DNA. To overcome these technical hiatuses we developed a Cre-d...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-7-12
更新日期:2007-03-01 00:00:00
abstract:BACKGROUND:A major obstacle to industrial-scale astaxanthin production by the yeast Phaffia rhodozyma is the strong inhibitory effect of high glucose concentration on astaxanthin synthesis. We investigated, for the first time, the mechanism of the regulatory effect of high glucose (> 100 g/L) at the metabolite and tran...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1507-6
更新日期:2019-06-15 00:00:00
abstract:BACKGROUND:Fungi are the second most abundant type of human pathogens. Invasive fungal pathogens are leading causes of life-threatening infections in clinical settings. Toxicity to the host and drug-resistance are two major deleterious issues associated with existing antifungal agents. Increasing a host's tolerance and...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-224
更新日期:2013-10-07 00:00:00
abstract:BACKGROUND:The plasma membrane plays an essential role in selective permeability, compartmentalization, osmotic balance, and cellular uptake. The characteristics and functions of cyanobacterial membranes have been extensively investigated in recent years. Cell-penetrating peptides (CPPs) are special nanocarriers that c...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-57
更新日期:2013-03-14 00:00:00
abstract:BACKGROUND:Enterococci are increasingly associated with opportunistic infections in Humans but the role of the oral cavity as a reservoir for this species is unclear. This study aimed to explore the carriage rate of Enterococci in the oral cavity of Tunisian children and their antimicrobial susceptibility to a broad ra...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-11-155
更新日期:2011-06-29 00:00:00
abstract:BACKGROUND:For a good probiotic candidate, the abilities to adhere to intestinal epithelium and to fortify barrier function are considered to be crucial for colonization and functionality of the strain. The strain Lactobacillus acidophilus LAB20 was isolated from the jejunum of a healthy dog, where it was found to be t...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-014-0337-9
更新日期:2015-01-16 00:00:00
abstract:BACKGROUND:Meiotic cells undergo two rounds of nuclear division and generate gametes. Previous studies have indicated that a number of transcription factors modulate the transcriptome in successive waves during meiosis and spore formation in fission yeast. However, the mechanisms underlying the post-transcriptional reg...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-14-188
更新日期:2014-07-15 00:00:00
abstract:BACKGROUND:Candida parapsilosis typically is a commensal of human skin. However, when host immune defense is compromised or the normal microflora balance is disrupted, C. parapsilosis transforms itself into an opportunistic pathogen. Candida-derived lipase has been identified as potential virulence factor. Even though ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-11-122
更新日期:2011-05-29 00:00:00
abstract:BACKGROUND:There is a rapid emergence of multiple resistant gram-negative bacteria due to overuse of antibiotics in the treatment of infections. Biofilms consist of polymicrobial communities that survive the host's defense system. The key bacteria in biofilms are slow growing and support an attachment and rapid growth ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0821-5
更新日期:2016-09-09 00:00:00
abstract:BACKGROUND:Moraxella catarrhalis is a human-specific gram-negative bacterium readily isolated from the respiratory tract of healthy individuals. The organism also causes significant health problems, including 15-20% of otitis media cases in children and ~10% of respiratory infections in adults with chronic obstructive ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-140
更新日期:2013-06-19 00:00:00
abstract:BACKGROUND:The influence of the membrane-bound AAA+ protease FtsH on membrane and cytoplasmic proteins of Corynebacterium glutamicum was investigated in this study. For the analysis of the membrane fraction, anion exchange chromatography was combined with SDS-PAGE, while the cytoplasmic protein fraction was studied by ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-7-6
更新日期:2007-01-25 00:00:00