Virulence regulator AphB enhances toxR transcription in Vibrio cholerae.

Abstract:

BACKGROUND:Vibrio cholerae is the causative agent of cholera. Extensive studies reveal that complicated regulatory cascades regulate expression of virulence genes, the products of which are required for V. cholerae to colonize and cause disease. In this study, we investigated the expression of the key virulence regulator ToxR under different conditions. RESULTS:We found that compared to that of wild type grown to stationary phase, the toxR expression was lower in an aphB mutant strain. AphB has been previously shown to be a key virulence regulator that is required to activate the expression of tcpP. When expressed constitutively, AphB is able to activate the toxR promoter. Furthermore, gel shift analysis indicates that AphB binds toxR promoter region directly. We also characterize the effect of AphB on the levels of the outer membrane porins OmpT and OmpU, which are known to be regulated by ToxR. CONCLUSIONS:Our data indicate that V. cholerae possesses an additional regulatory loop that use AphB to activate the expression of two virulence regulators, ToxR and TcpP, which together control the expression of the master virulence regulator ToxT.

journal_name

BMC Microbiol

journal_title

BMC microbiology

authors

Xu X,Stern AM,Liu Z,Kan B,Zhu J

doi

10.1186/1471-2180-10-3

subject

Has Abstract

pub_date

2010-01-06 00:00:00

pages

3

issn

1471-2180

pii

1471-2180-10-3

journal_volume

10

pub_type

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