Abstract：
BACKGROUND:Non-structural protein 1 (NS1) is a multifunctional protein and a crucial regulatory factor in the replication and pathogenesis of avian influenza virus (AIV). Studies have shown that NS1 can interact with a variety of host proteins to modulate the viral life cycle. We previously generated a monoclonal antibody against NS1 protein; In the current research study, using this antibody, we immunoprecipitated host proteins that interact with NS1 to better understand the roles played by NS1 in communications between virus and host. RESULTS:Co-immunoprecipitation experiments identified annexin A2 (ANXA2) as a target molecule interacting with NS1. Results from confocal laser scanning microscopy indicated that NS1 co-localized with ANXA2 in the cell cytoplasm. Overexpression of ANXA2 significantly increased the titer of H5N1 subtype HPAIV, whereas siRNA-mediated knockdown of ANXA2 markedly inhibited the expression of viral proteins and reduced the progeny virus titer. CONCLUSIONS:Our results indicate that ANXA2 interacts with NS1 and ANXA2 expression increases HPAIV replication.
journal_name
BMC Microbioljournal_title
BMC microbiologyauthors
Ma Y,Sun J,Gu L,Bao H,Zhao Y,Shi L,Yao W,Tian G,Wang X,Chen Hdoi
10.1186/s12866-017-1097-0subject
Has Abstractpub_date
2017-09-11 00:00:00pages
191issue
1issn
1471-2180pii
10.1186/s12866-017-1097-0journal_volume
17pub_type
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