Release of extracellular ATP by bacteria during growth.

Abstract:

BACKGROUND:Adenosine triphosphate (ATP) is used as an intracellular energy source by all living organisms. It plays a central role in the respiration and metabolism, and is the most important energy supplier in many enzymatic reactions. Its critical role as the energy storage molecule makes it extremely valuable to all cells. RESULTS:We report here the detection of extracellular ATP in the cultures of a variety of bacterial species. The levels of the extracellular ATP in bacterial cultures peaked around the end of the log phase and decreased in the stationary phase of growth. Extracellular ATP levels were dependent on the cellular respiration as bacterial mutants lacking cytochrome bo oxidase displayed lower extracellular ATP levels. We have also shown that Escherichia coli (E. coli) and Salmonella actively depleted extracellular ATP and an ATP supplement in culture media enhanced the stationary survival of E. coli and Salmonella. In addition to E. coli and Salmonella the presence of the extracellular ATP was observed in a variety of bacterial species that contain human pathogens such as Acinetobacter, Pseudomonas, Klebsiella and Staphylococcus. CONCLUSION:Our results indicate that extracellular ATP is produced by many bacterial species during growth and extracellular ATP may serve a role in the bacterial physiology.

journal_name

BMC Microbiol

journal_title

BMC microbiology

authors

Mempin R,Tran H,Chen C,Gong H,Kim Ho K,Lu S

doi

10.1186/1471-2180-13-301

subject

Has Abstract

pub_date

2013-12-24 00:00:00

pages

301

issn

1471-2180

pii

1471-2180-13-301

journal_volume

13

pub_type

杂志文章
  • Holdfast spreading and thickening during Caulobacter crescentus attachment to surfaces.

    abstract:BACKGROUND:Adhesion to surfaces facilitates many crucial functions of microbes in their natural habitats. Thus understanding the mechanism of microbial adhesion is of broad interest to the microbiology research community. RESULTS:We report a study by fluorescence imaging and atomic force microscopy on the growth in si...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-139

    authors: Li G,Brun YV,Tang JX

    更新日期:2013-06-19 00:00:00

  • Characterization and partial purification of Candida albicans Secretory IL-12 Inhibitory Factor.

    abstract:BACKGROUND:We have previously shown that supernatant from Candida albicans (CA) culture contains a Secretory Interleukin (IL)-12 Inhibitory Factor (CA-SIIF), which inhibits IL-12 production by human monocytes. However, the effect of CA-SIIF on secretion of other cytokines by monocytes is unknown, and detailed character...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-31

    authors: Wang M,Mukherjee PK,Chandra J,Lattif AA,McCormick TS,Ghannoum MA

    更新日期:2008-02-19 00:00:00

  • A blue native-PAGE analysis of membrane protein complexes in Clostridium thermocellum.

    abstract:BACKGROUND:Clostridium thermocellum is a Gram-positive thermophilic anaerobic bacterium with the unusual capacity to convert cellulosic biomass into ethanol and hydrogen. Identification and characterization of protein complexes in C. thermocellum are important toward understanding its metabolism and physiology. RESULT...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-11-22

    authors: Peng Y,Luo Y,Yu T,Xu X,Fan K,Zhao Y,Yang K

    更新日期:2011-01-26 00:00:00

  • Bactericidal activity of PA-824 against Mycobacterium tuberculosis under anaerobic conditions and computational analysis of its novel analogues against mutant Ddn receptor.

    abstract:BACKGROUND:The resurgence of multi-drug resistant tuberculosis (MDR-TB) and HIV associated tuberculosis (TB) are of serious global concern. To contain this situation, new anti-tuberculosis drugs and reduced treatment regimens are imperative. Recently, a nitroimidazole, PA-824, has been shown to be active against both r...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-218

    authors: Somasundaram S,Anand RS,Venkatesan P,Paramasivan CN

    更新日期:2013-10-01 00:00:00

  • Sequence analysis of percent G+C fraction libraries of human faecal bacterial DNA reveals a high number of Actinobacteria.

    abstract:BACKGROUND:The human gastrointestinal (GI) tract microbiota is characterised by an abundance of uncultured bacteria most often assigned in phyla Firmicutes and Bacteroidetes. Diversity of this microbiota, even though approached with culture independent techniques in several studies, still requires more elucidation. The...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-68

    authors: Krogius-Kurikka L,Kassinen A,Paulin L,Corander J,Mäkivuokko H,Tuimala J,Palva A

    更新日期:2009-04-08 00:00:00

  • Repertoire of the gut microbiota from stomach to colon using culturomics and next-generation sequencing.

    abstract:BACKGROUND:Most studies on the human microbiota have analyzed stool samples, although a large proportion of the absorption of nutrients takes place in upper gut tract. We collected samples from different locations along the entire gastrointestinal tract from six patients who had simultaneously undergone upper endoscopy...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1304-7

    authors: Mailhe M,Ricaboni D,Vitton V,Gonzalez JM,Bachar D,Dubourg G,Cadoret F,Robert C,Delerce J,Levasseur A,Fournier PE,Angelakis E,Lagier JC,Raoult D

    更新日期:2018-10-24 00:00:00

  • Effect of different drugs and drug combinations on killing stationary phase and biofilms recovered cells of Bartonella henselae in vitro.

    abstract:BACKGROUND:Bartonella henselae is a Gram-negative bacterium transmitted to humans by a scratch from cat in the presence of ectoparasites. Humans infected with B. henselae can result in various clinical diseases including local lymphadenopathy and more serious systemic disease such as persistent bacteremia and endocardi...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01777-9

    authors: Zheng X,Ma X,Li T,Shi W,Zhang Y

    更新日期:2020-04-10 00:00:00

  • Comparative analysis of the growth and biological activity of a respiratory and atheroma isolate of Chlamydia pneumoniae reveals strain-dependent differences in inflammatory activity and innate immune evasion.

    abstract:BACKGROUND:Chlamydia pneumoniae is a common human pathogen that is associated with upper and lower respiratory tract infections. It has also been suggested that C. pneumoniae infection can trigger or promote a number of chronic inflammatory conditions, including asthma and atherosclerosis. Several strains of C. pneumon...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0569-3

    authors: He X,Liang Y,LaValley MP,Lai J,Ingalls RR

    更新日期:2015-10-23 00:00:00

  • In vivo bioluminescence imaging of the spatial and temporal colonization of lactobacillus plantarum 423 and enterococcus mundtii ST4SA in the intestinal tract of mice.

    abstract:BACKGROUND:Lactic acid bacteria (LAB) are major inhabitants and part of the normal microflora of the gastrointestinal tract (GIT) of humans and animals. Despite substantial evidence supporting the beneficial properties of LAB, only a few studies have addressed the migration and colonization of probiotic bacteria in the...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1315-4

    authors: Van Zyl WF,Deane SM,Dicks LMT

    更新日期:2018-10-30 00:00:00

  • Loss of malic enzymes leads to metabolic imbalance and altered levels of trehalose and putrescine in the bacterium Sinorhizobium meliloti.

    abstract:BACKGROUND:Malic enzymes decarboxylate the tricarboxylic acid (TCA) cycle intermediate malate to the glycolytic end-product pyruvate and are well positioned to regulate metabolic flux in central carbon metabolism. Despite the wide distribution of these enzymes, their biological roles are unclear in part because the rea...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0780-x

    authors: Zhang Y,Smallbone LA,diCenzo GC,Morton R,Finan TM

    更新日期:2016-07-26 00:00:00

  • In vivo bioluminescence imaging and histopathopathologic analysis reveal distinct roles for resident and recruited immune effector cells in defense against invasive aspergillosis.

    abstract:BACKGROUND:Invasive aspergillosis (IA) is a major cause of infectious morbidity and mortality in immune compromised patients. Studies on the pathogenesis of IA have been limited by the difficulty to monitor disease progression in real-time. For real-time monitoring of the infection, we recently engineered a bioluminesc...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-105

    authors: Ibrahim-Granet O,Jouvion G,Hohl TM,Droin-Bergère S,Philippart F,Kim OY,Adib-Conquy M,Schwendener R,Cavaillon JM,Brock M

    更新日期:2010-04-08 00:00:00

  • Genetic stability of Brucella abortus isolates from an outbreak by multiple-locus variable-number tandem repeat analysis (MLVA16).

    abstract:BACKGROUND:Brucellosis caused by Brucella abortus is one of the most important zoonoses in the world. Multiple-locus variable-number tandem repeat analysis (MLVA16) has been shown be a useful tool to epidemiological traceback studies in B. abortus infection. Thus, the present study aimed (i) to evaluate the genetic div...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-14-186

    authors: Dorneles EM,Santana JA,Alves TM,Pauletti RB,Mol JP,Heinemann MB,Lage AP

    更新日期:2014-07-11 00:00:00

  • Mycobacterium abscessus multispacer sequence typing.

    abstract:BACKGROUND:Mycobacterium abscessus group includes antibiotic-resistant, opportunistic mycobacteria that are responsible for sporadic cases and outbreaks of cutaneous, pulmonary and disseminated infections. However, because of their close genetic relationships, accurate discrimination between the various strains of thes...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-3

    authors: Sassi M,Ben Kahla I,Drancourt M

    更新日期:2013-01-07 00:00:00

  • Ribosomal subunit protein typing using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the identification and discrimination of Aspergillus species.

    abstract:BACKGROUND:Accurate identification of Aspergillus species is a very important subject. Mass spectral fingerprinting using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is generally employed for the rapid identification of fungal isolates. However, the results are based on s...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-017-1009-3

    authors: Nakamura S,Sato H,Tanaka R,Kusuya Y,Takahashi H,Yaguchi T

    更新日期:2017-04-26 00:00:00

  • Photorhabdus adhesion modification protein (Pam) binds extracellular polysaccharide and alters bacterial attachment.

    abstract:BACKGROUND:Photorhabdus are Gram-negative nematode-symbiotic and insect-pathogenic bacteria. The species Photorhabdus asymbiotica is able to infect humans as well as insects. We investigated the secreted proteome of a clinical isolate of P. asymbiotica at different temperatures in order to identify proteins relevant to...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-141

    authors: Jones RT,Sanchez-Contreras M,Vlisidou I,Amos MR,Yang G,Muñoz-Berbel X,Upadhyay A,Potter UJ,Joyce SA,Ciche TA,Jenkins AT,Bagby S,Ffrench-Constant RH,Waterfield NR

    更新日期:2010-05-12 00:00:00

  • Did granny know best? Evaluating the antibacterial, antifungal and antiviral efficacy of acetic acid for home care procedures.

    abstract:BACKGROUND:Acetic acid has been used to clean and disinfect surfaces in the household for many decades. The antimicrobial efficacy of cleaning procedures can be considered particularly important for young, old, pregnant, immunocompromised people, but may also concern other groups, particularly with regards to the COVID...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01948-8

    authors: Zinn MK,Bockmühl D

    更新日期:2020-08-26 00:00:00

  • Development of a rapid and simplified protocol for direct bacterial identification from positive blood cultures by using matrix assisted laser desorption ionization time-of- flight mass spectrometry.

    abstract:BACKGROUND:Bloodstream infections represent serious conditions carrying a high mortality and morbidity rate. Rapid identification of microorganisms and prompt institution of adequate antimicrobial therapy is of utmost importance for a successful outcome. Aiming at the development of a rapid, simplified and efficient pr...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0594-2

    authors: Jakovljev A,Bergh K

    更新日期:2015-11-06 00:00:00

  • Lab on a chip genotyping for Brucella spp. based on 15-loci multi locus VNTR analysis.

    abstract:BACKGROUND:Brucellosis is an important zoonosis caused by the genus Brucella. In addition Brucella represents potential biological warfare agents due to the high contagious rates for humans and animals. Therefore, the strain typing epidemiological tool may be crucial for tracing back source of infection in outbreaks an...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-66

    authors: De Santis R,Ciammaruconi A,Faggioni G,D'Amelio R,Marianelli C,Lista F

    更新日期:2009-04-07 00:00:00

  • Biofilm formation as a novel phenotypic feature of adherent-invasive Escherichia coli (AIEC).

    abstract:BACKGROUND:Crohn's disease (CD) is a high morbidity chronic inflammatory disorder of unknown aetiology. Adherent-invasive Escherichia coli (AIEC) has been recently implicated in the origin and perpetuation of CD. Because bacterial biofilms in the gut mucosa are suspected to play a role in CD and biofilm formation is a ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-202

    authors: Martinez-Medina M,Naves P,Blanco J,Aldeguer X,Blanco JE,Blanco M,Ponte C,Soriano F,Darfeuille-Michaud A,Garcia-Gil LJ

    更新日期:2009-09-21 00:00:00

  • Common origin of plasmid encoded alpha-hemolysin genes in Escherichia coli.

    abstract:BACKGROUND:Alpha (alpha)-hemolysin is a pore forming cytolysin and serves as a virulence factor in intestinal and extraintestinal pathogenic strains of E. coli. It was suggested that the genes encoding alpha-hemolysin (hlyCABD) which can be found on the chromosome and plasmid, were acquired through horizontal gene tran...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-193

    authors: Burgos Y,Beutin L

    更新日期:2010-07-19 00:00:00

  • The process-related dynamics of microbial community during a simulated fermentation of Chinese strong-flavored liquor.

    abstract:BACKGROUND:Famous Chinese strong-flavored liquor (CSFL) is brewed by microbial consortia in a special fermentation pit (FT). However, the fermentation process was not fully understood owing to the complicate community structure and metabolism. In this study, the process-related dynamics of microbial communities and mai...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-017-1106-3

    authors: Zhang Y,Zhu X,Li X,Tao Y,Jia J,He X

    更新日期:2017-09-15 00:00:00

  • Resistance to topoisomerase cleavage complex induced lethality in Escherichia coli via titration of transcription regulators PurR and FNR.

    abstract:BACKGROUND:Accumulation of gyrase cleavage complex in Escherichia coli from the action of quinolone antibiotics induces an oxidative damage cell death pathway. The oxidative cell death pathway has also been shown to be involved in the lethality following accumulation of cleavage complex formed by bacterial topoisomeras...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-11-261

    authors: Liu IF,Aedo S,Tse-Dinh YC

    更新日期:2011-12-12 00:00:00

  • Bifidobacteria strains isolated from stools of iron deficient infants can efficiently sequester iron.

    abstract:BACKGROUND:Bifidobacteria is one of the major gut commensal groups found in infants. Their colonization is commonly associated with beneficial effects to the host through mechanisms like niche occupation and nutrient competition against pathogenic bacteria. Iron is an essential element necessary for most microorganisms...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-014-0334-z

    authors: Vazquez-Gutierrez P,Lacroix C,Jaeggi T,Zeder C,Zimmerman MB,Chassard C

    更新日期:2015-01-16 00:00:00

  • Genetic identity and differential gene expression between Trichomonas vaginalis and Trichomonas tenax.

    abstract:BACKGROUND:Trichomonas vaginalis is a human urogenital pathogen responsible for trichomonosis, the number-one, non-viral sexually transmitted disease (STD) worldwide, while T. tenax is a commensal of the human oral cavity, found particularly in patients with poor oral hygiene and advanced periodontal disease. The exten...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-58

    authors: Kucknoor AS,Mundodi V,Alderete J

    更新日期:2009-03-18 00:00:00

  • Special issue on enhancing vector refractoriness to trypanosome infection-foreword.

    abstract:: ...

    journal_title:BMC microbiology

    pub_type:

    doi:10.1186/s12866-018-1279-4

    authors: Abd-Alla AMM,Tsiamis G,Boucias DG

    更新日期:2018-11-23 00:00:00

  • Long-term impact of fecal transplantation in healthy volunteers.

    abstract:BACKGROUND:Fecal microbiota transplantation (FMT) has been recently approved by FDA for the treatment of refractory recurrent clostridial colitis (rCDI). Success of FTM in treatment of rCDI led to a number of studies investigating the effectiveness of its application in the other gastrointestinal diseases. However, in ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1689-y

    authors: Goloshchapov OV,Olekhnovich EI,Sidorenko SV,Moiseev IS,Kucher MA,Fedorov DE,Pavlenko AV,Manolov AI,Gostev VV,Veselovsky VA,Klimina KM,Kostryukova ES,Bakin EA,Shvetcov AN,Gumbatova ED,Klementeva RV,Shcherbakov AA,Gorchak

    更新日期:2019-12-30 00:00:00

  • Adhesive polypeptides of Staphylococcus aureus identified using a novel secretion library technique in Escherichia coli.

    abstract:BACKGROUND:Bacterial adhesive proteins, called adhesins, are frequently the decisive factor in initiation of a bacterial infection. Characterization of such molecules is crucial for the understanding of bacterial pathogenesis, design of vaccines and development of antibacterial drugs. Because adhesins are frequently di...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-11-117

    authors: Kylväjä R,Kankainen M,Holm L,Westerlund-Wikström B

    更新日期:2011-05-27 00:00:00

  • Susceptibility of Pediococcus isolates to antimicrobial compounds in relation to hop-resistance and beer-spoilage.

    abstract:BACKGROUND:Though important in the context of food microbiology and as potential pathogens in immuno-compromised humans, bacterial isolates belonging to the genus Pediococcus are best known for their association with contamination of ethanol fermentation processes (beer, wine, or fuel ethanol). Use of antimicrobial com...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-190

    authors: Haakensen M,Vickers DM,Ziola B

    更新日期:2009-09-07 00:00:00

  • Contribution of the non-effector members of the HrpL regulon, iaaL and matE, to the virulence of Pseudomonas syringae pv. tomato DC3000 in tomato plants.

    abstract:BACKGROUND:The phytohormone indole-3-acetic acid (IAA) is widely distributed among plant-associated bacteria. Certain strains of the Pseudomonas syringae complex can further metabolize IAA into a less biologically active amino acid conjugate, 3-indole-acetyl-ε-L-lysine, through the action of the iaaL gene. In P. syring...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0503-8

    authors: Castillo-Lizardo MG,Aragón IM,Carvajal V,Matas IM,Pérez-Bueno ML,Gallegos MT,Barón M,Ramos C

    更新日期:2015-08-19 00:00:00

  • In vitro model of production of antibodies; a new approach to reveal the presence of key bacteria in polymicrobial environments.

    abstract:BACKGROUND:There is a rapid emergence of multiple resistant gram-negative bacteria due to overuse of antibiotics in the treatment of infections. Biofilms consist of polymicrobial communities that survive the host's defense system. The key bacteria in biofilms are slow growing and support an attachment and rapid growth ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0821-5

    authors: Wu C,Nakka S,Mansouri S,Bengtsson T,Nayeri T,Nayeri F

    更新日期:2016-09-09 00:00:00