Abstract:
BACKGROUND:It is unclear how DNA is packaged in a bacterial cell in the absence of nucleosomes. To investigate the initial level of DNA condensation in bacterial nucleoid we used in vivo DNA digestion coupled with high-throughput sequencing of the digestion-resistant fragments. To this end, we transformed E. coli cells with a plasmid expressing micrococcal nuclease. The nuclease expression was under the control of AraC repressor, which enabled us to perform an inducible digestion of bacterial nucleoid inside a living cell. RESULTS:Analysis of the genomic localization of the digestion-resistant fragments revealed their non-random distribution. The patterns observed in the distribution of the sequenced fragments indicate the presence of short DNA segments protected from the enzyme digestion, possibly because of interaction with DNA-binding proteins. The average length of such digestion-resistant segments is about 50 bp and the characteristic repeat in their distribution is about 90 bp. The gene starts are depleted of the digestion-resistant fragments, suggesting that these genomic regions are more exposed than genomic sequences on average. Sequence analysis of the digestion-resistant segments showed that while the GC-content of such sequences is close to the genome-wide value, they are depleted of A-tracts as compared to the bulk genomic DNA or to the randomized sequence of the same nucleotide composition. CONCLUSIONS:Our results suggest that DNA is packaged in the bacterial nucleoid in a non-random way that facilitates interaction of the DNA binding factors with regulatory regions of the genome.
journal_name
BMC Microbioljournal_title
BMC microbiologyauthors
Tolstorukov MY,Virnik K,Zhurkin VB,Adhya Sdoi
10.1186/s12866-016-0637-3subject
Has Abstractpub_date
2016-02-20 00:00:00pages
22issn
1471-2180pii
10.1186/s12866-016-0637-3journal_volume
16pub_type
杂志文章相关文献
BMC MICROBIOLOGY文献大全abstract:BACKGROUND:Flavobacterium columnare (Bacteroidetes) is the causative agent of columnaris disease in farmed freshwater fish around the world. The bacterium forms three colony morphotypes (Rhizoid, Rough and Soft), but the differences of the morphotypes are poorly known. We studied the virulence of the morphotypes produc...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-14-170
更新日期:2014-06-26 00:00:00
abstract:BACKGROUND:Quorum sensing is a communication system that regulates gene expression in response to population density and often regulates virulence determinants. Deletion of the luxR homologue vjbR highly attenuates intracellular survival of Brucella melitensis and has been interpreted to be an indication of a role for ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-167
更新日期:2010-06-08 00:00:00
abstract:BACKGROUND:Enteropathogenic Escherichia coli (EPEC) are classified into typical and atypical strains based on the presence of the E. coli adherence factor (EAF) plasmid. The EAF plasmid contains the bfp (bundle-forming pilus) operon and the perABC (plasmid encoded regulator) gene cluster. A 1-kb cryptic region of EAF p...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0539-9
更新日期:2015-10-05 00:00:00
abstract:BACKGROUND:The emergence of antibiotic resistant pathogenic bacteria has reduced our ability to combat infectious diseases. At the same time the numbers of new antibiotics reaching the market have decreased. This situation has created an urgent need to discover novel antibiotic scaffolds. Recently, the application of p...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0696-5
更新日期:2016-05-10 00:00:00
abstract:BACKGROUND:Escherichia coli strains are commonly found in the gut microflora of warm-blooded animals. These strains can be assigned to one of the four main phylogenetic groups, A, B1, B2 and D, which can be divided into seven subgroups (A0, A1, B1, B22, B23, D1 and D2), according to the combination of the three genetic...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-161
更新日期:2010-06-01 00:00:00
abstract:BACKGROUND:The Uzon Caldera is one of the places on our planet with unique geological, ecological, and microbiological characteristics. Uzon oil is the youngest on Earth. Uzon oil has unique composition, with low proportion of heavy fractions and relatively high content of saturated hydrocarbons. Microbial communities ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-02012-1
更新日期:2020-11-24 00:00:00
abstract:BACKGROUND:Since bacteria embedded in biofilms are far more difficult to eradicate than planktonic infections, it would be useful to know whether certain Staphylococcus aureus lineages are especially involved in strong biofilm formation. For this reason, in vitro biofilm formation of 228 clinical S. aureus isolates of ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-229
更新日期:2009-10-28 00:00:00
abstract:BACKGROUND:Predicting antibiotic resistance before it emerges at clinical settings constitutes a novel approach for preventing and fighting resistance of bacterial pathogens. To analyse the possibility that novel plasmid-encoded quinolone resistance determinants (Qnr) can emerge and disseminate among bacterial pathogen...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-148
更新日期:2008-09-16 00:00:00
abstract:BACKGROUND:Generation of extended- spectrum β- lactamases is one of the major mechanisms by which clinical Klebsiella pneumoniae develop resistance to antibiotics. Combined antibiotics prove to be a relatively effective method of controlling such resistant strains. Some of Chinese herbal active ingredients are known to...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0797-1
更新日期:2016-08-08 00:00:00
abstract:BACKGROUND:The dspA (hik33) gene, coding for a putative sensory histidine kinase, is conserved in plastids (ycf26) and cyanobacteria. It has been linked with a number of different stress responses in cyanobacteria. RESULTS:We constructed an insertional mutant of dspA (ycf26) in Synechocystis 6803. We found little phen...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-5-47
更新日期:2005-08-02 00:00:00
abstract:BACKGROUND:Most of the studies evaluating the secular trends of blood isolates come from tertiary hospitals in urban areas. We sought to study the trends of the antimicrobial resistance of blood isolates in patients from a rural population hospitalized in a tertiary hospital in a small city in Greece. METHODS:We retro...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-6-41
更新日期:2006-05-02 00:00:00
abstract:BACKGROUND:Establishing an optimal proportion of nitrifying microbial populations, including ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB), complete nitrite oxidizers (comammox) and ammonia-oxidizing archaea (AOA), is important for ensuring the efficiency of nitrification in water treatment systems...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-0998-2
更新日期:2017-04-04 00:00:00
abstract::Protein secretion plays a central role in modulating the interactions of bacteria with their environments. This is particularly the case when symbiotic bacteria (whether pathogenic, commensal or mutualistic) are interacting with larger host organisms. In the case of Gram-negative bacteria, secretion requires transloca...
journal_title:BMC microbiology
pub_type: 杂志文章,评审
doi:10.1186/1471-2180-9-S1-S2
更新日期:2009-02-19 00:00:00
abstract:BACKGROUND:The catabolite control protein CcpA is a transcriptional regulator conserved in many Gram-positives, controlling the efficiency of glucose metabolism. Here we studied the role of Bacillus cereus ATCC 14579 CcpA in regulation of metabolic pathways and expression of enterotoxin genes by comparative transcripto...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-62
更新日期:2008-04-16 00:00:00
abstract:BACKGROUND:Porphyromonas gingivalis, a microorganism residing in the oral cavity within complex multispecies biofilms, is one of the keystone pathogens in the onset and progression of periodontitis. In this in vitro study, using DNA microarray, we investigate the differential gene expression of Porphyromonas gingivalis...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1423-9
更新日期:2019-03-12 00:00:00
abstract:BACKGROUND:Streptococcus pseudopneumoniae, is a novel member of the genus Streptococcus, falling close to related members like S. pneumoniae, S. mitis, and S. oralis. Its recent appearance has shed light on streptococcal infections, which has been unclear till recently. In this study, the transcriptome of S. pseudopneu...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-77
更新日期:2012-07-06 00:00:00
abstract:BACKGROUND:Cryptosporidium spp. and Giardia duodenalis are important causes of diarrheal diseases in humans and animals worldwide, and there is an increased interest in the role of animals in the mechanical transmission of these protozoa. To examine the role of yaks in this process, we examined the occurrence and genot...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0446-0
更新日期:2015-05-21 00:00:00
abstract:BACKGROUND:The Bacillus anthracis S-layer protein, BslA, plays a crucial role in mammalian infection. BslA is required to mediate adherence between host cells and vegetative forms of bacteria and this interaction promotes target organs adherence and blood-brain barrier (BBB) penetration in vivo. This study attempts to ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0802-8
更新日期:2016-08-11 00:00:00
abstract:BACKGROUND:During the last two decades research on animal filarial parasites, especially Onchocerca ochengi, infecting cattle in savanna areas of Africa revealed that O. ochengi as an animal model has biological features that are similar to those of O. volvulus, the aetiological agent of human onchocerciasis. There is,...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-02064-3
更新日期:2021-01-06 00:00:00
abstract:BACKGROUND:Bacteria belonging to Planctomycetes display several unique morphological and genetic features and are found in a wide variety of habitats on earth. Their ecological roles in these habitats are still poorly understood. Planctomycetes have previously been detected throughout the year on surfaces of the kelp L...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-261
更新日期:2010-10-15 00:00:00
abstract:BACKGROUND:The compatible solute trehalose is involved in the osmostress response of Rhizobium etli, the microsymbiont of Phaseolus vulgaris. In this work, we reconstructed trehalose metabolism in R. etli, and investigated its role in cellular adaptation and survival to heat and desiccation stress under free living con...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-207
更新日期:2012-09-17 00:00:00
abstract:BACKGROUND:The balanced synthesis of membrane phospholipids, fatty acids and cell wall constituents is a vital facet of bacterial physiology, but there is little known about the biochemical control points that coordinate these activities in Gram-positive bacteria. In Escherichia coli, the glycerol-phosphate acyltransfe...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-260
更新日期:2013-11-16 00:00:00
abstract:BACKGROUND:Alpha (alpha)-hemolysin is a pore forming cytolysin and serves as a virulence factor in intestinal and extraintestinal pathogenic strains of E. coli. It was suggested that the genes encoding alpha-hemolysin (hlyCABD) which can be found on the chromosome and plasmid, were acquired through horizontal gene tran...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-193
更新日期:2010-07-19 00:00:00
abstract:BACKGROUND:The phenomenon of misdiagnosing tuberculosis (TB) by laboratory cross-contamination when culturing Mycobacterium tuberculosis (MTB) has been widely reported and it has an obvious clinical, therapeutic and social impact. The final confirmation of a cross-contamination event requires the molecular identificati...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-30
更新日期:2008-02-14 00:00:00
abstract:BACKGROUND:The naked mole-rat (NMR, Heterocephalus glaber) is being bred as a novel laboratory animal due to its unique biological characteristics, including longevity, cancer resistance, hypoxia tolerance, and pain insensitivity. It is expected that differences exist between the microbiota of wild NMRs and that of NMR...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1226-4
更新日期:2018-08-22 00:00:00
abstract:BACKGROUND:Papua New Guinea (PNG) is a high tuberculosis (TB) burden country of the WHO Western Pacific Region, but so far research on drug resistance (DR) and genotypes of Mycobacterium tuberculosis (M. tuberculosis) was only conducted in few provinces in the country. The aim of the present study was to obtain baselin...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-014-0307-2
更新日期:2014-12-05 00:00:00
abstract:BACKGROUND:The natural product Emodin demonstrates a wide range of pharmacological properties including anticancer, anti-inflammatory, antiproliferation, vasorelaxant and anti-H. pylori activities. Although its H. pylori inhibition was discovered, no acting target information against Emodin has been revealed to date. ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-91
更新日期:2009-05-12 00:00:00
abstract:BACKGROUND:The resurgence of multi-drug resistant tuberculosis (MDR-TB) and HIV associated tuberculosis (TB) are of serious global concern. To contain this situation, new anti-tuberculosis drugs and reduced treatment regimens are imperative. Recently, a nitroimidazole, PA-824, has been shown to be active against both r...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-218
更新日期:2013-10-01 00:00:00
abstract:BACKGROUND:The species Pectobacterium carotovorum includes a diverse subspecies of bacteria that cause disease on a wide variety of plants. In Morocco, approximately 95% of the P. carotovorum isolates from potato plants with tuber soft rot are P. carotovorum subsp. carotovorum. However, identification of this pathogen ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-176
更新日期:2013-07-29 00:00:00
abstract:BACKGROUND:Bovicin HC5 is an antimicrobial peptide that shows a broad spectrum of activity and potential for biotechnological and therapeutic applications. To gain insight about the safety of bovicin HC5 application, the histological and immunostimulatory effects of orally administrated bovicin HC5 to BALB/c mice were ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-69
更新日期:2013-03-27 00:00:00