Diversity of Mycobacterium tuberculosis and drug resistance in different provinces of Papua New Guinea.

Abstract:

BACKGROUND:Papua New Guinea (PNG) is a high tuberculosis (TB) burden country of the WHO Western Pacific Region, but so far research on drug resistance (DR) and genotypes of Mycobacterium tuberculosis (M. tuberculosis) was only conducted in few provinces in the country. The aim of the present study was to obtain baseline data on the level of drug resistance and the genotypic diversity of circulating M. tuberculosis in additional provinces and to investigate the differences between three selected sites across PNG. RESULTS:Genotyping of 147 M. tuberculosis clinical isolates collected in Goroka, Eastern Highlands Province, in Alotau, Milne Bay Province and in Madang, Madang Province revealed three main lineages of M. tuberculosis: Lineage 4 (European-American lineage), Lineage 2 (East-Asian lineage) and Lineage 1 (Indo-Oceanic lineage). All three lineages were detected in all three sites, but the individual lineage compositions varied significantly between sites. In Madang Lineage 4 was the most prevalent lineage (76.6%), whereas in Goroka and Alotau Lineage 2 was dominating (60.5% and 84.4%, respectively) (p < 0.001). Overall, phenotypic drug susceptibility testing showed 10.8% resistance to at least one of the first-line drugs tested. Of all resistant strains (23/212) 30.4% were Streptomycin mono-resistant, 17.4% were Isoniazid mono-resistant and 13% were Rifampicin mono-resistant. Multi-drug resistant (MDR) TB was found in 2.8% of all tested cases (6/212). The highest amount of MDR TB was found in Alotau in Milne Bay Province (4.6%). CONCLUSION:A large number of drug resistant TB infections are present in the country and MDR TB has already been detected in all three surveyed regions of PNG, highlighting the importance of monitoring drug resistance and making it a high priority for the National Control Program. Due to the high prevalence of Lineage 2 in Milne Bay Province and given the frequent association of this lineage with drug resistance, monitoring of the latter should especially be scaled up in that province.

journal_name

BMC Microbiol

journal_title

BMC microbiology

authors

Ley SD,Harino P,Vanuga K,Kamus R,Carter R,Coulter C,Pandey S,Feldmann J,Ballif M,Siba PM,Phuanukoonnon S,Gagneux S,Beck HP

doi

10.1186/s12866-014-0307-2

subject

Has Abstract

pub_date

2014-12-05 00:00:00

pages

307

issn

1471-2180

pii

s12866-014-0307-2

journal_volume

14

pub_type

杂志文章
  • Common and specific genomic sequences of avian and human extraintestinal pathogenic Escherichia coli as determined by genomic subtractive hybridization.

    abstract:BACKGROUND:Suppression subtractive hybridization (SSH) strategy was used with extraintestinal pathogenic Escherichia coli (EXPEC) that cause avian colibacillosis (avian pathogenic E. coli or APEC) and human urinary tract infections (uropathogenic E. coli or UPEC) to determine if they possessed genes that were host and/...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-7-81

    authors: Kariyawasam S,Scaccianoce JA,Nolan LK

    更新日期:2007-08-30 00:00:00

  • CCL5 regulation of mucosal chlamydial immunity and infection.

    abstract:BACKGROUND:Following genital chlamydial infection, an early T helper type 1 (Th1)-associated immune response precedes the activation and recruitment of specific Th1 cells bearing distinct chemokine receptors, subsequently leading to the clearance of Chlamydia. We have shown that CCR5, a receptor for CCL5, is crucial fo...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-136

    authors: Sakthivel SK,Singh UP,Singh S,Taub DD,Igietseme JU,Lillard JW Jr

    更新日期:2008-08-13 00:00:00

  • A microbiome case-control study of recurrent acute otitis media identified potentially protective bacterial genera.

    abstract:BACKGROUND:Recurrent acute otitis media (rAOM, recurrent ear infection) is a common childhood disease caused by bacteria termed otopathogens, for which current treatments have limited effectiveness. Generic probiotic therapies have shown promise, but seem to lack specificity. We hypothesised that healthy children with ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1154-3

    authors: Lappan R,Imbrogno K,Sikazwe C,Anderson D,Mok D,Coates H,Vijayasekaran S,Bumbak P,Blyth CC,Jamieson SE,Peacock CS

    更新日期:2018-02-20 00:00:00

  • Evolutionary relationships of ATP-Binding Cassette (ABC) uptake porters.

    abstract:BACKGROUND:The ATP-Binding Cassette (ABC) functional superfamily includes integral transmembrane exporters that have evolved three times independently, forming three families termed ABC1, ABC2 and ABC3, upon which monophyletic ATPases have been superimposed for energy-coupling purposes [e.g., J Membr Biol 231(1):1-10, ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-98

    authors: Zheng WH,Västermark Å,Shlykov MA,Reddy V,Sun EI,Saier MH Jr

    更新日期:2013-05-06 00:00:00

  • Colonization of Enteroaggregative Escherichia coli and Shiga toxin-producing Escherichia coli in chickens and humans in southern Vietnam.

    abstract:BACKGROUND:Enteroaggregative (EAEC) and Shiga-toxin producing Escherichia coli (STEC) are a major cause of diarrhea worldwide. E. coli carrying both virulence factors characteristic for EAEC and STEC and producing extended-spectrum beta-lactamase caused severe and protracted disease during an outbreak of E. coli O104:H...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0827-z

    authors: Trung NV,Nhung HN,Carrique-Mas JJ,Mai HH,Tuyen HT,Campbell J,Nhung NT,Van Minh P,Wagenaar JA,Mai NT,Hieu TQ,Schultsz C,Hoa NT

    更新日期:2016-09-09 00:00:00

  • Assessing diversity of the female urine microbiota by high throughput sequencing of 16S rDNA amplicons.

    abstract:BACKGROUND:Urine within the urinary tract is commonly regarded as "sterile" in cultivation terms. Here, we present a comprehensive in-depth study of bacterial 16S rDNA sequences associated with urine from healthy females by means of culture-independent high-throughput sequencing techniques. RESULTS:Sequencing of the V...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-11-244

    authors: Siddiqui H,Nederbragt AJ,Lagesen K,Jeansson SL,Jakobsen KS

    更新日期:2011-11-02 00:00:00

  • Oxygen stress reduces zoospore survival of Phytophthora species in a simulated aquatic system.

    abstract:BACKGROUND:The genus Phytophthora includes a group of agriculturally important pathogens and they are commonly regarded as water molds. They produce motile zoospores that can move via water currents and on their own locomotion in aquatic environments. However, zoosporic response to dissolved oxygen, an important water ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-14-124

    authors: Kong P,Hong C

    更新日期:2014-05-13 00:00:00

  • Carbon and arsenic metabolism in Thiomonas strains: differences revealed diverse adaptation processes.

    abstract:BACKGROUND:Thiomonas strains are ubiquitous in arsenic-contaminated environments. Differences between Thiomonas strains in the way they have adapted and respond to arsenic have never been studied in detail. For this purpose, five Thiomonas strains, that are interesting in terms of arsenic metabolism were selected: T. a...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-127

    authors: Bryan CG,Marchal M,Battaglia-Brunet F,Kugler V,Lemaitre-Guillier C,Lièvremont D,Bertin PN,Arsène-Ploetze F

    更新日期:2009-06-23 00:00:00

  • Correction to: Identification of cholesterol-assimilating actinomycetes strain and application of statistical modeling approaches for improvement of cholesterol oxidase production by Streptomyces anulatus strain NEAE-94.

    abstract::Following publication of the original article [1], the authors reported an error in Fig. 2c. ...

    journal_title:BMC microbiology

    pub_type: 已发布勘误

    doi:10.1186/s12866-020-01791-x

    authors: El-Naggar NE,El-Shweihy NM

    更新日期:2020-04-30 00:00:00

  • Community analysis of biofilms on flame-oxidized stainless steel anodes in microbial fuel cells fed with different substrates.

    abstract:BACKGROUND:The flame-oxidized stainless steel anode (FO-SSA) is a newly developed electrode that enhances microbial fuel cell (MFC) power generation; however, substrate preference and community structure of the biofilm developed on FO-SSA have not been well characterized. Herein, we investigated the community on FO-SSA...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-017-1053-z

    authors: Eyiuche NJ,Asakawa S,Yamashita T,Ikeguchi A,Kitamura Y,Yokoyama H

    更新日期:2017-06-29 00:00:00

  • Geochip-based analysis of microbial communities in alpine meadow soils in the Qinghai-Tibetan plateau.

    abstract:BACKGROUND:GeoChip 3.0, a microbial functional gene array, containing ~28,000 oligonucleotide probes and targeting ~57,000 sequences from 292 functional gene families, provided a powerful tool for researching microbial community structure in natural environments. The alpine meadow is a dominant plant community in the Q...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-72

    authors: Zhang Y,Lu Z,Liu S,Yang Y,He Z,Ren Z,Zhou J,Li D

    更新日期:2013-03-29 00:00:00

  • Bactericidal activity of PA-824 against Mycobacterium tuberculosis under anaerobic conditions and computational analysis of its novel analogues against mutant Ddn receptor.

    abstract:BACKGROUND:The resurgence of multi-drug resistant tuberculosis (MDR-TB) and HIV associated tuberculosis (TB) are of serious global concern. To contain this situation, new anti-tuberculosis drugs and reduced treatment regimens are imperative. Recently, a nitroimidazole, PA-824, has been shown to be active against both r...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-218

    authors: Somasundaram S,Anand RS,Venkatesan P,Paramasivan CN

    更新日期:2013-10-01 00:00:00

  • Inducible Siphoviruses in superficial and deep tissue isolates of Propionibacterium acnes.

    abstract:BACKGROUND:Propionibacterium acnes is a commensal of human skin but is also known to be involved in certain diseases, such as acne vulgaris and infections of orthopaedic implants. Treatment of these conditions is complicated by increased resistance to antibiotics and/or biofilm formation of P. acnes bacteria. P. acnes ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-139

    authors: Lood R,Mörgelin M,Holmberg A,Rasmussen M,Collin M

    更新日期:2008-08-15 00:00:00

  • Quantitative differential proteomics of yeast extracellular matrix: there is more to it than meets the eye.

    abstract:BACKGROUND:Saccharomyces cerevisiae multicellular communities are sustained by a scaffolding extracellular matrix, which provides spatial organization, and nutrient and water availability, and ensures group survival. According to this tissue-like biology, the yeast extracellular matrix (yECM) is analogous to the higher...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0550-1

    authors: Faria-Oliveira F,Carvalho J,Ferreira C,Hernáez ML,Gil C,Lucas C

    更新日期:2015-11-25 00:00:00

  • Organization of DNA in a bacterial nucleoid.

    abstract:BACKGROUND:It is unclear how DNA is packaged in a bacterial cell in the absence of nucleosomes. To investigate the initial level of DNA condensation in bacterial nucleoid we used in vivo DNA digestion coupled with high-throughput sequencing of the digestion-resistant fragments. To this end, we transformed E. coli cells...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0637-3

    authors: Tolstorukov MY,Virnik K,Zhurkin VB,Adhya S

    更新日期:2016-02-20 00:00:00

  • Outer membrane protein P4 is not required for virulence in the human challenge model of Haemophilus ducreyi infection.

    abstract:BACKGROUND:Bacterial lipoproteins often play important roles in pathogenesis and can stimulate protective immune responses. Such lipoproteins are viable vaccine candidates. Haemophilus ducreyi, which causes the sexually transmitted disease chancroid, expresses a number of lipoproteins during human infection. One such l...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-14-166

    authors: Janowicz DM,Zwickl BW,Fortney KR,Katz BP,Bauer ME

    更新日期:2014-06-24 00:00:00

  • A recombinase system facilitates cloning of expression cassettes in the ciliate Tetrahymena thermophila.

    abstract:BACKGROUND:Tetrahymena thermophila is one of the best characterized unicellular eukaryotes and its genome is sequenced in its entirety. However, the AT-richness of the genome and an unusual codon usage cause problems in cloning and expression of the ciliate DNA. To overcome these technical hiatuses we developed a Cre-d...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-7-12

    authors: Weide T,Bockau U,Rave A,Herrmann L,Hartmann MW

    更新日期:2007-03-01 00:00:00

  • Resistance to topoisomerase cleavage complex induced lethality in Escherichia coli via titration of transcription regulators PurR and FNR.

    abstract:BACKGROUND:Accumulation of gyrase cleavage complex in Escherichia coli from the action of quinolone antibiotics induces an oxidative damage cell death pathway. The oxidative cell death pathway has also been shown to be involved in the lethality following accumulation of cleavage complex formed by bacterial topoisomeras...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-11-261

    authors: Liu IF,Aedo S,Tse-Dinh YC

    更新日期:2011-12-12 00:00:00

  • High taxonomic level fingerprint of the human intestinal microbiota by ligase detection reaction--universal array approach.

    abstract:BACKGROUND:Affecting the core functional microbiome, peculiar high level taxonomic unbalances of the human intestinal microbiota have been recently associated with specific diseases, such as obesity, inflammatory bowel diseases, and intestinal inflammation. RESULTS:In order to specifically monitor microbiota unbalance...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-116

    authors: Candela M,Consolandi C,Severgnini M,Biagi E,Castiglioni B,Vitali B,De Bellis G,Brigidi P

    更新日期:2010-04-19 00:00:00

  • Diversity and transcription of proteases involved in the maturation of hydrogenases in Nostoc punctiforme ATCC 29133 and Nostoc sp. strain PCC 7120.

    abstract:BACKGROUND:The last step in the maturation process of the large subunit of [NiFe]-hydrogenases is a proteolytic cleavage of the C-terminal by a hydrogenase specific protease. Contrary to other accessory proteins these hydrogenase proteases are believed to be specific whereby one type of hydrogenases specific protease o...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-53

    authors: Devine E,Holmqvist M,Stensjö K,Lindblad P

    更新日期:2009-03-11 00:00:00

  • Microbial iron management mechanisms in extremely acidic environments: comparative genomics evidence for diversity and versatility.

    abstract:BACKGROUND:Iron is an essential nutrient but can be toxic at high intracellular concentrations and organisms have evolved tightly regulated mechanisms for iron uptake and homeostasis. Information on iron management mechanisms is available for organisms living at circumneutral pH. However, very little is known about how...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-203

    authors: Osorio H,Martínez V,Nieto PA,Holmes DS,Quatrini R

    更新日期:2008-11-24 00:00:00

  • Effect of deletion of gene cluster involved in synthesis of Enterobacterial common antigen on virulence and immunogenicity of live attenuated Salmonella vaccine when delivering heterologous Streptococcus pneumoniae antigen PspA.

    abstract:BACKGROUND:Enterobacterial common antigen (ECA) is a family-specific surface antigen shared by all members of the Enterobacteriaceae family. Previous studies showed that the loss of ECA results in Salmonella attenuation, indicating its usefulness as a vaccine candidate for Salmonella infection, but no studies have show...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01837-0

    authors: Liu Q,Shen X,Bian X,Kong Q

    更新日期:2020-06-08 00:00:00

  • Structure, Genetics and Worldwide Spread of New Delhi Metallo-β-lactamase (NDM): a threat to public health.

    abstract:BACKGROUND:The emergence of carbapenemase producing bacteria, especially New Delhi metallo-β-lactamase (NDM-1) and its variants, worldwide, has raised amajor public health concern. NDM-1 hydrolyzes a wide range of β-lactam antibiotics, including carbapenems, which are the last resort of antibiotics for the treatment of...

    journal_title:BMC microbiology

    pub_type: 杂志文章,评审

    doi:10.1186/s12866-017-1012-8

    authors: Khan AU,Maryam L,Zarrilli R

    更新日期:2017-04-27 00:00:00

  • Analysis of virulence phenotypes and antibiotic resistance in clinical strains of Acinetobacter baumannii isolated in Nashville, Tennessee.

    abstract:BACKGROUND:Acinetobacter baumannii is a gram-negative bacterium which causes opportunistic infections in immunocompromised hosts. Genome plasticity has given rise to a wide range of strain variation with respect to antimicrobial resistance profiles and expression of virulence factors which lead to altered phenotypes as...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-02082-1

    authors: Boone RL,Whitehead B,Avery TM,Lu J,Francis JD,Guevara MA,Moore RE,Chambers SA,Doster RS,Manning SD,Townsend SD,Dent L,Marshall D,Gaddy JA,Damo SM

    更新日期:2021-01-09 00:00:00

  • Oxygen restriction increases the infective potential of Listeria monocytogenes in vitro in Caco-2 cells and in vivo in guinea pigs.

    abstract:BACKGROUND:Listeria monocytogenes has been implicated in several food borne outbreaks as well as sporadic cases of disease. Increased understanding of the biology of this organism is important in the prevention of food borne listeriosis. The infectivity of Listeria monocytogenes ScottA, cultivated with and without oxyg...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-7-55

    authors: Bo Andersen J,Roldgaard BB,Christensen BB,Licht TR

    更新日期:2007-06-14 00:00:00

  • High-level chromate resistance in Arthrobacter sp. strain FB24 requires previously uncharacterized accessory genes.

    abstract:BACKGROUND:The genome of Arthrobacter sp. strain FB24 contains a chromate resistance determinant (CRD), consisting of a cluster of 8 genes located on a 10.6 kb fragment of a 96 kb plasmid. The CRD includes chrA, which encodes a putative chromate efflux protein, and three genes with amino acid similarities to the amino ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-199

    authors: Henne KL,Nakatsu CH,Thompson DK,Konopka AE

    更新日期:2009-09-16 00:00:00

  • Did granny know best? Evaluating the antibacterial, antifungal and antiviral efficacy of acetic acid for home care procedures.

    abstract:BACKGROUND:Acetic acid has been used to clean and disinfect surfaces in the household for many decades. The antimicrobial efficacy of cleaning procedures can be considered particularly important for young, old, pregnant, immunocompromised people, but may also concern other groups, particularly with regards to the COVID...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01948-8

    authors: Zinn MK,Bockmühl D

    更新日期:2020-08-26 00:00:00

  • ChMob2 binds to ChCbk1 and promotes virulence and conidiation of the fungal pathogen Colletotrichum higginsianum.

    abstract:BACKGROUND:Mob family proteins are conserved between animals, plants and fungi and are essential for the activation of NDR kinases that control crucial cellular processes like cytokinesis, proliferation and morphology. RESULTS:We identified a hypomorphic allele of ChMOB2 in a random insertional mutant (vir-88) of the ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-017-0932-7

    authors: Schmidpeter J,Dahl M,Hofmann J,Koch C

    更新日期:2017-01-19 00:00:00

  • Evaluation of prokaryotic diversity of five hot springs in Eritrea.

    abstract:BACKGROUND:Total community rDNA was used to determine the diversity of bacteria and archaea from water, wet sediment and microbial mats samples of hot springs in the Eastern lowlands of Eritrea. The temperatures of the springs range from 49.5 °C to 100 °C while pH levels varied from 6.97 to 7.54. Akwar and Maiwooi have...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-017-1113-4

    authors: Ghilamicael AM,Budambula NLM,Anami SE,Mehari T,Boga HI

    更新日期:2017-09-22 00:00:00

  • Cow manure application effectively regulates the soil bacterial community in tea plantation.

    abstract:BACKGROUND:Cow manure is not only an agricultural waste, but also an organic fertilizer resource. The application of organic fertilizer is a feasible practice to mitigate the soil degradation caused by overuse of chemical fertilizers, which can affect the bacterial diversity and community composition in soils. However,...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01871-y

    authors: Zhang S,Sun L,Wang Y,Fan K,Xu Q,Li Y,Ma Q,Wang J,Ren W,Ding Z

    更新日期:2020-07-01 00:00:00