An easy, simple inexpensive test for the specific detection of Pectobacterium carotovorum subsp. carotovorum based on sequence analysis of the pmrA gene.

Abstract:

BACKGROUND:The species Pectobacterium carotovorum includes a diverse subspecies of bacteria that cause disease on a wide variety of plants. In Morocco, approximately 95% of the P. carotovorum isolates from potato plants with tuber soft rot are P. carotovorum subsp. carotovorum. However, identification of this pathogen is not always related to visual disease symptoms. This is especially true when different pathogen cause similar diseases on potato, citing as an example, P. carotovorum, P. atrosepticum and P. wasabiae. Numerous conventional methods were used to characterize Pectobacterium spp., including biochemical assays, specific PCR-based tests, and construction of phylogenetic trees by using gene sequences. In this study, an alternative method is presented using a gene linked to pathogenicity, in order to allow accuracy at subspecies level. The pmrA gene (response regulator) has been used for identification and analysis of the relationships among twenty nine Pectobacterium carotovorum subsp. carotovorum and other Pectobacterium subspecies. RESULTS:Phylogenetic analyses of pmrA sequences compared to ERIC-PCR and 16S rDNA sequencing, demonstrated that there is considerable genetic diversity in P. carotovorum subsp. carotovorum strains, which can be divided into two distinct groups within the same clade. CONCLUSIONS:pmrA sequence analysis is likely to be a reliable tool to identify the subspecies Pectobacterium carotovorum subsp. carotovorum and estimate their genetic diversity.

journal_name

BMC Microbiol

journal_title

BMC microbiology

authors

Kettani-Halabi M,Terta M,Amdan M,El Fahime el M,Bouteau F,Ennaji MM

doi

10.1186/1471-2180-13-176

subject

Has Abstract

pub_date

2013-07-29 00:00:00

pages

176

issn

1471-2180

pii

1471-2180-13-176

journal_volume

13

pub_type

杂志文章
  • The effects of daily fasting hours on shaping gut microbiota in mice.

    abstract:BACKGROUND:It has recently been reported that intermittent fasting shapes the gut microbiota to benefit health, but this effect may be influenced to the exact fasting protocols. The purpose of this study was to assess the effects of different daily fasting hours on shaping the gut microbiota in mice. Healthy C57BL/6 J ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01754-2

    authors: Li L,Su Y,Li F,Wang Y,Ma Z,Li Z,Su J

    更新日期:2020-03-24 00:00:00

  • The antibacterial effect of nitric oxide against ESBL-producing uropathogenic E. coli is improved by combination with miconazole and polymyxin B nonapeptide.

    abstract:BACKGROUND:Nitric oxide (NO) is produced as part of the host immune response to bacterial infections, including urinary tract infections. The enzyme flavohemoglobin, coded by the hmp gene, is involved in protecting bacterial cells from the toxic effects of NO and represents a potentially interesting target for developm...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-14-65

    authors: Bang CS,Kinnunen A,Karlsson M,Önnberg A,Söderquist B,Persson K

    更新日期:2014-03-14 00:00:00

  • Deregulation of phytoene-β-carotene synthase results in derepression of astaxanthin synthesis at high glucose concentration in Phaffia rhodozyma astaxanthin-overproducing strain MK19.

    abstract:BACKGROUND:A major obstacle to industrial-scale astaxanthin production by the yeast Phaffia rhodozyma is the strong inhibitory effect of high glucose concentration on astaxanthin synthesis. We investigated, for the first time, the mechanism of the regulatory effect of high glucose (> 100 g/L) at the metabolite and tran...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1507-6

    authors: Miao L,Chi S,Wu M,Liu Z,Li Y

    更新日期:2019-06-15 00:00:00

  • Analysis and comparison of the pan-genomic properties of sixteen well-characterized bacterial genera.

    abstract:BACKGROUND:The increasing availability of whole genome sequences allows the gene or protein content of different organisms to be compared, leading to burgeoning interest in the relatively new subfield of pan-genomics. However, while several studies have analyzed protein content relationships in specific groups of bacte...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-258

    authors: Trost B,Haakensen M,Pittet V,Ziola B,Kusalik A

    更新日期:2010-10-13 00:00:00

  • Azithromycin effectiveness against intracellular infections of Francisella.

    abstract:BACKGROUND:Macrolide antibiotics are commonly administered for bacterial respiratory illnesses. Azithromycin (Az) is especially noted for extremely high intracellular concentrations achieved within macrophages which is far greater than the serum concentration. Clinical strains of Type B Francisella (F.) tularensis have...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-123

    authors: Ahmad S,Hunter L,Qin A,Mann BJ,van Hoek ML

    更新日期:2010-04-23 00:00:00

  • Assessment of CcpA-mediated catabolite control of gene expression in Bacillus cereus ATCC 14579.

    abstract:BACKGROUND:The catabolite control protein CcpA is a transcriptional regulator conserved in many Gram-positives, controlling the efficiency of glucose metabolism. Here we studied the role of Bacillus cereus ATCC 14579 CcpA in regulation of metabolic pathways and expression of enterotoxin genes by comparative transcripto...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-62

    authors: van der Voort M,Kuipers OP,Buist G,de Vos WM,Abee T

    更新日期:2008-04-16 00:00:00

  • Analysis of virulence phenotypes and antibiotic resistance in clinical strains of Acinetobacter baumannii isolated in Nashville, Tennessee.

    abstract:BACKGROUND:Acinetobacter baumannii is a gram-negative bacterium which causes opportunistic infections in immunocompromised hosts. Genome plasticity has given rise to a wide range of strain variation with respect to antimicrobial resistance profiles and expression of virulence factors which lead to altered phenotypes as...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-02082-1

    authors: Boone RL,Whitehead B,Avery TM,Lu J,Francis JD,Guevara MA,Moore RE,Chambers SA,Doster RS,Manning SD,Townsend SD,Dent L,Marshall D,Gaddy JA,Damo SM

    更新日期:2021-01-09 00:00:00

  • Protein expression dynamics during Escherichia coli glucose-lactose diauxie.

    abstract:BACKGROUND:Escherichia coli is a well-studied anaerobic bacteria which is able to regulate metabolic pathways depending on the type of sugar presented in the medium. We have studied the glucose-lactose shift in E. coli at the protein level using a recently developed mass spectrometry platform. METHOD:Cells were grown ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-11-126

    authors: Mostovenko E,Deelder AM,Palmblad M

    更新日期:2011-06-01 00:00:00

  • Genome characterization and population genetic structure of the zoonotic pathogen, Streptococcus canis.

    abstract:BACKGROUND:Streptococcus canis is an important opportunistic pathogen of dogs and cats that can also infect a wide range of additional mammals including cows where it can cause mastitis. It is also an emerging human pathogen. RESULTS:Here we provide characterization of the first genome sequence for this species, strai...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-12-293

    authors: Richards VP,Zadoks RN,Pavinski Bitar PD,Lefébure T,Lang P,Werner B,Tikofsky L,Moroni P,Stanhope MJ

    更新日期:2012-12-18 00:00:00

  • Oxygen stress reduces zoospore survival of Phytophthora species in a simulated aquatic system.

    abstract:BACKGROUND:The genus Phytophthora includes a group of agriculturally important pathogens and they are commonly regarded as water molds. They produce motile zoospores that can move via water currents and on their own locomotion in aquatic environments. However, zoosporic response to dissolved oxygen, an important water ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-14-124

    authors: Kong P,Hong C

    更新日期:2014-05-13 00:00:00

  • Community analysis of biofilms on flame-oxidized stainless steel anodes in microbial fuel cells fed with different substrates.

    abstract:BACKGROUND:The flame-oxidized stainless steel anode (FO-SSA) is a newly developed electrode that enhances microbial fuel cell (MFC) power generation; however, substrate preference and community structure of the biofilm developed on FO-SSA have not been well characterized. Herein, we investigated the community on FO-SSA...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-017-1053-z

    authors: Eyiuche NJ,Asakawa S,Yamashita T,Ikeguchi A,Kitamura Y,Yokoyama H

    更新日期:2017-06-29 00:00:00

  • Rapidly disseminating blaOXA-232 carrying Klebsiella pneumoniae belonging to ST231 in India: multiple and varied mobile genetic elements.

    abstract:BACKGROUND:Recently, in India, there has been a shift from NDM to OXA48-like carbapenemases. OXA-181 and OXA-232 are the frequently produced variants of OXA48-like carbapenemases. OXA48-like carbapenemases are also known to be carried on transposons such as Tn1999, Tn1999.2 and it is also associated with IS1R carried o...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1513-8

    authors: Shankar C,Mathur P,Venkatesan M,Pragasam AK,Anandan S,Khurana S,Veeraraghavan B

    更新日期:2019-06-24 00:00:00

  • Modelling invasive group A streptococcal disease using bioluminescence.

    abstract:BACKGROUND:The development of vaccines and evaluation of novel treatment strategies for invasive group A streptococcal (iGAS) disease requires suitable models of human infection that can be monitored longitudinally and are preferably non-invasive. Bio-photonic imaging provides an opportunity to reduce use of animals in...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1200-1

    authors: Lamb LE,Zhi X,Alam F,Pyzio M,Scudamore CL,Wiles S,Sriskandan S

    更新日期:2018-06-19 00:00:00

  • Genetic identity and differential gene expression between Trichomonas vaginalis and Trichomonas tenax.

    abstract:BACKGROUND:Trichomonas vaginalis is a human urogenital pathogen responsible for trichomonosis, the number-one, non-viral sexually transmitted disease (STD) worldwide, while T. tenax is a commensal of the human oral cavity, found particularly in patients with poor oral hygiene and advanced periodontal disease. The exten...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-58

    authors: Kucknoor AS,Mundodi V,Alderete J

    更新日期:2009-03-18 00:00:00

  • Distribution and diversity of mycoplasma plasmids: lessons from cryptic genetic elements.

    abstract:BACKGROUND:The evolution of mycoplasmas from a common ancestor with Firmicutes has been characterized not only by genome down-sizing but also by horizontal gene transfer between mycoplasma species sharing a common host. The mechanisms of these gene transfers remain unclear because our knowledge of the mycoplasma mobile...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-12-257

    authors: Breton M,Tardy F,Dordet-Frisoni E,Sagne E,Mick V,Renaudin J,Sirand-Pugnet P,Citti C,Blanchard A

    更新日期:2012-11-12 00:00:00

  • Study on the community structure and function of symbiotic bacteria from different growth and developmental stages of Hypsizygus marmoreus.

    abstract:BACKGROUND:The symbiotic bacteria associated with edible fungi are valuable microbial resources worthy of in-depth exploration. It is important to analyze the community structure and succession of symbiotic bacteria in mushrooms. This can assist in the isolation of growth-promoting strains that have an essential relati...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01998-y

    authors: Sun S,Li F,Xu X,Liu Y,Kong X,Chen J,Liu T,Chen L

    更新日期:2020-10-14 00:00:00

  • Survey of bacterial diversity in chronic wounds using pyrosequencing, DGGE, and full ribosome shotgun sequencing.

    abstract:BACKGROUND:Chronic wound pathogenic biofilms are host-pathogen environments that colonize and exist as a cohabitation of many bacterial species. These bacterial populations cooperate to promote their own survival and the chronic nature of the infection. Few studies have performed extensive surveys of the bacterial popu...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-43

    authors: Dowd SE,Sun Y,Secor PR,Rhoads DD,Wolcott BM,James GA,Wolcott RD

    更新日期:2008-03-06 00:00:00

  • Ribosomal subunit protein typing using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the identification and discrimination of Aspergillus species.

    abstract:BACKGROUND:Accurate identification of Aspergillus species is a very important subject. Mass spectral fingerprinting using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is generally employed for the rapid identification of fungal isolates. However, the results are based on s...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-017-1009-3

    authors: Nakamura S,Sato H,Tanaka R,Kusuya Y,Takahashi H,Yaguchi T

    更新日期:2017-04-26 00:00:00

  • A comparison of the Giardia lamblia trophozoite and cyst transcriptome using microarrays.

    abstract:BACKGROUND:Compared with many protists, Giardia lamblia has a simple life cycle alternating between cyst and trophozoite. Most research on the molecular biology of Giardia parasites has focused on trophozoites and the processes of excystation and encystation, whereas cysts have attracted less interest. The striking mor...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-11-91

    authors: Faghiri Z,Widmer G

    更新日期:2011-05-04 00:00:00

  • Isocitrate dehydrogenase mutation in Vibrio anguillarum results in virulence attenuation and immunoprotection in rainbow trout (Oncorhynchus mykiss).

    abstract:BACKGROUND:Vibrio anguillarum is an extracellular bacterial pathogen that is a causative agent of vibriosis in finfish and crustaceans with mortality rates ranging from 30% to 100%. Mutations in central metabolism (glycolysis and the TCA cycle) of intracellular pathogens often result in attenuated virulence due to depl...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-017-1124-1

    authors: Mou X,Spinard EJ,Hillman SL,Nelson DR

    更新日期:2017-11-14 00:00:00

  • Roles of iron acquisition systems in virulence of extraintestinal pathogenic Escherichia coli: salmochelin and aerobactin contribute more to virulence than heme in a chicken infection model.

    abstract:BACKGROUND:Avian pathogenic Escherichia coli (APEC) and uropathogenic E. coli (UPEC) are the two main subsets of extraintestinal pathogenic E. coli (ExPEC). Both types have multiple iron acquisition systems, including heme and siderophores. Although iron transport systems involved in the pathogenesis of APEC or UPEC ha...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-12-143

    authors: Gao Q,Wang X,Xu H,Xu Y,Ling J,Zhang D,Gao S,Liu X

    更新日期:2012-07-20 00:00:00

  • In vitro modeling of host-parasite interactions: the 'subgingival' biofilm challenge of primary human epithelial cells.

    abstract:BACKGROUND:Microbial biofilms are known to cause an increasing number of chronic inflammatory and infectious conditions. A classical example is chronic periodontal disease, a condition initiated by the subgingival dental plaque biofilm on gingival epithelial tissues. We describe here a new model that permits the examin...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-280

    authors: Guggenheim B,Gmür R,Galicia JC,Stathopoulou PG,Benakanakere MR,Meier A,Thurnheer T,Kinane DF

    更新日期:2009-12-31 00:00:00

  • The surface protein Shr of Streptococcus pyogenes binds heme and transfers it to the streptococcal heme-binding protein Shp.

    abstract:BACKGROUND:The heme acquisition machinery in Streptococcus pyogenes is believed to consist of the surface proteins, Shr and Shp, and heme-specific ATP-binding cassette transporter HtsABC. Shp has been shown to rapidly transfer its heme to the lipoprotein component, HtsA, of HtsABC. The function of Shr and the heme sour...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-15

    authors: Zhu H,Liu M,Lei B

    更新日期:2008-01-23 00:00:00

  • Robustness analysis of culturing perturbations on Escherichia coli colony biofilm beta-lactam and aminoglycoside antibiotic tolerance.

    abstract:BACKGROUND:Biofilms are ubiquitous. For instance, the majority of medical infections are thought to involve biofilms. However even after decades of investigation, the in vivo efficacy of many antimicrobial strategies is still debated suggesting there is a need for better understanding of biofilm antimicrobial tolerance...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-185

    authors: Zuroff TR,Bernstein H,Lloyd-Randolfi J,Jimenez-Taracido L,Stewart PS,Carlson RP

    更新日期:2010-07-07 00:00:00

  • Identification and characterization of intervening sequences within 23S rRNA genes from more than 200 Campylobacter isolates from seven species including atypical campylobacters.

    abstract:BACKGROUND:Identification and characterization of intervening sequences (IVSs) within 23S rRNA genes from Campylobacter organisms including atypical campylobacters were carried out using two PCR primer pairs, designed to generate helix 25 and 45 regions. RESULTS:Only C. sputorum biovar sputorum LMG7975 and fecalis LMG...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-256

    authors: Tazumi A,Kakinuma Y,Misawa N,Moore JE,Millar BC,Matsuda M

    更新日期:2009-12-11 00:00:00

  • Insights into the impact of flhF inactivation on Campylobacter jejuni colonization of chick and mice gut.

    abstract:BACKGROUND:Campylobacter jejuni (C. jejuni) is a leading cause of foodborne gastroenteritis worldwide. This bacterium lacks many of the classical virulence factors, and flagellum-associated persistent colonization has been shown to be crucial for its pathogenesis. The flagellum plays a multifunctional role in C. jejuni...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1318-1

    authors: Ren F,Li X,Tang H,Jiang Q,Yun X,Fang L,Huang P,Tang Y,Li Q,Huang J,Jiao XA

    更新日期:2018-10-22 00:00:00

  • Genetic diversity and characterization of arsenic-resistant endophytic bacteria isolated from Pteris vittata, an arsenic hyperaccumulator.

    abstract:BACKGROUND:Alleviating arsenic (As) contamination is a high-priority environmental issue. Hyperaccumulator plants may harbor endophytic bacteria able to detoxify As. Therefore, we investigated the distribution, diversity, As (III) resistance levels, and resistance-related functional genes of arsenite-resistant bacteria...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1184-x

    authors: Gu Y,Wang Y,Sun Y,Zhao K,Xiang Q,Yu X,Zhang X,Chen Q

    更新日期:2018-05-08 00:00:00

  • CoBaltDB: Complete bacterial and archaeal orfeomes subcellular localization database and associated resources.

    abstract:BACKGROUND:The functions of proteins are strongly related to their localization in cell compartments (for example the cytoplasm or membranes) but the experimental determination of the sub-cellular localization of proteomes is laborious and expensive. A fast and low-cost alternative approach is in silico prediction, bas...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-88

    authors: Goudenège D,Avner S,Lucchetti-Miganeh C,Barloy-Hubler F

    更新日期:2010-03-23 00:00:00

  • Production of infectious HCV genotype 1b virus in cell culture using a novel Set of adaptive mutations.

    abstract:BACKGROUND:Despite the high prevalence of genotype 1b hepatitis C virus (HCV) among patients, a cell culture system that permits entire viral life cycle of genotype 1b isolates is limited. To develop a cell-cultured hepatitis C virus (HCVcc) of genotype 1b, the proper combination of HCV genomic variants and host cells ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0846-9

    authors: Mori KI,Matsumoto A,Maki N,Ichikawa Y,Tanaka E,Yagi S

    更新日期:2016-09-27 00:00:00

  • Correction to: Identification of cholesterol-assimilating actinomycetes strain and application of statistical modeling approaches for improvement of cholesterol oxidase production by Streptomyces anulatus strain NEAE-94.

    abstract::Following publication of the original article [1], the authors reported an error in Fig. 2c. ...

    journal_title:BMC microbiology

    pub_type: 已发布勘误

    doi:10.1186/s12866-020-01791-x

    authors: El-Naggar NE,El-Shweihy NM

    更新日期:2020-04-30 00:00:00