当前位置： SCI文献检索 > BMC MICROBIOLOGY期刊下所有文献 > The surface protein Shr of Streptococcus pyogenes binds heme and transfers it to the streptococcal heme-binding protein Shp.

The surface protein Shr of Streptococcus pyogenes binds heme and transfers it to the streptococcal heme-binding protein Shp.

Abstract：

BACKGROUND:The heme acquisition machinery in Streptococcus pyogenes is believed to consist of the surface proteins, Shr and Shp, and heme-specific ATP-binding cassette transporter HtsABC. Shp has been shown to rapidly transfer its heme to the lipoprotein component, HtsA, of HtsABC. The function of Shr and the heme source of Shp have not been established. RESULTS:The objective of this study was to determine whether Shr binds heme and is a heme source of Shp. To achieve the objective, recombinant Shr protein was prepared. The purified Shr displays a spectrum typical of hemoproteins, indicating that Shr binds heme and acquires heme from Escherichia coli hemoproteins in vivo. Spectral analysis of Shr and Shp isolated from a mixture of Shr and heme-free Shp (apoShp) indicates that Shr and apoShp lost and gained heme, respectively; whereas Shr did not efficiently lose its heme in incubation with apoHtsA under the identical conditions. These results suggest that Shr directly transfers its heme to Shp. In addition, the rates of heme transfer from human hemoglobin to apoShp are close to those of simple ferric heme dissociation from hemoglobin, suggesting that methemoglobin does not directly transfer its heme to apoShp. CONCLUSION:We have demonstrated that recombinant Shr can acquire heme from E. coli hemoproteins in vivo and appears to directly transfer its heme to Shp and that Shp appears not to directly acquire heme from human methemoglobin. These results suggest the possibility that Shr is a source of heme for Shp and that the Shr-to-Shp heme transfer is a step of the heme acquisition process in S. pyogenes. Further characterization of the Shr/Shp/HtsA system would advance our understanding of the mechanism of heme acquisition in S. pyogenes.

journal_name

BMC Microbiol

journal_title

BMC microbiology

authors

Zhu H,Liu M,Lei B

doi

10.1186/1471-2180-8-15

subject

Has Abstract

pub_date

2008-01-23 00:00:00

pages

15

issn

1471-2180

pii

1471-2180-8-15

journal_volume

8

pub_type

杂志文章

相关文献

BMC MICROBIOLOGY文献大全
  • Chemical modulators of the innate immune response alter gypsy moth larval susceptibility to Bacillus thuringiensis.

    abstract：BACKGROUND:The gut comprises an essential barrier that protects both invertebrate and vertebrate animals from invasion by microorganisms. Disruption of the balanced relationship between indigenous gut microbiota and their host can result in gut bacteria eliciting host responses similar to those caused by invasive patho...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/1471-2180-10-129

    authors： Broderick NA,Raffa KF,Handelsman J

    更新日期：2010-04-27 00:00:00

  • Robustness analysis of culturing perturbations on Escherichia coli colony biofilm beta-lactam and aminoglycoside antibiotic tolerance.

    abstract：BACKGROUND:Biofilms are ubiquitous. For instance, the majority of medical infections are thought to involve biofilms. However even after decades of investigation, the in vivo efficacy of many antimicrobial strategies is still debated suggesting there is a need for better understanding of biofilm antimicrobial tolerance...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/1471-2180-10-185

    authors： Zuroff TR,Bernstein H,Lloyd-Randolfi J,Jimenez-Taracido L,Stewart PS,Carlson RP

    更新日期：2010-07-07 00:00:00

  • The biological effects of microencapsulated organic acids and botanicals induces tissue-specific and dose-dependent changes to the Gallus gallus microbiota.

    abstract：BACKGROUND:Microencapsulated organic acids and botanicals have the potential to develop into important tools for the poultry industry. A blend of organic acids and botanicals (AviPlus®P) has previously shown to reduce Salmonella and Campylobacter in chickens; however, changes to the microbiota of the jejunum and ileum ...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/s12866-020-02001-4

    authors： Feye KM,Swaggerty CL,Kogut MH,Ricke SC,Piva A,Grilli E

    更新日期：2020-11-02 00:00:00

  • Effect of different drugs and drug combinations on killing stationary phase and biofilms recovered cells of Bartonella henselae in vitro.

    abstract：BACKGROUND:Bartonella henselae is a Gram-negative bacterium transmitted to humans by a scratch from cat in the presence of ectoparasites. Humans infected with B. henselae can result in various clinical diseases including local lymphadenopathy and more serious systemic disease such as persistent bacteremia and endocardi...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/s12866-020-01777-9

    authors： Zheng X,Ma X,Li T,Shi W,Zhang Y

    更新日期：2020-04-10 00:00:00

  • Inducible Siphoviruses in superficial and deep tissue isolates of Propionibacterium acnes.

    abstract：BACKGROUND:Propionibacterium acnes is a commensal of human skin but is also known to be involved in certain diseases, such as acne vulgaris and infections of orthopaedic implants. Treatment of these conditions is complicated by increased resistance to antibiotics and/or biofilm formation of P. acnes bacteria. P. acnes ...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/1471-2180-8-139

    authors： Lood R,Mörgelin M,Holmberg A,Rasmussen M,Collin M

    更新日期：2008-08-15 00:00:00

  • Analysis of and function predictions for previously conserved hypothetical or putative proteins in Blochmannia floridanus.

    abstract：BACKGROUND:There is an increasing interest to better understand endosymbiont capabilities in insects both from an ecological point of view and for pest control. Blochmannia floridanus provides important nutrients for its host, the ant Camponotus, while the bacterium in return is provided with a niche to proliferate. Bl...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/1471-2180-6-1

    authors： Gaudermann P,Vogl I,Zientz E,Silva FJ,Moya A,Gross R,Dandekar T

    更新日期：2006-01-09 00:00:00

  • The synergetic effect of Imipenem-clarithromycin combination in the Mycobacteroides abscessus complex.

    abstract：BACKGROUND:Nontuberculous mycobacteria (NTM) are ubiquitous organisms and the incidence of NTM infections has been increasing in recent years. Mycobacteroides abscessus (M. abscessus) is one of the most antimicrobial-resistant NTM; however, no reliable antibiotic regimen can be officially advocated. We evaluated the ef...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/s12866-020-02000-5

    authors： Takei S,Ihara H,Togo S,Nakamura A,Fujimoto Y,Watanabe J,Kurokawa K,Shibayama K,Sumiyoshi I,Ochi Y,Iwai M,Okabe T,Chonan M,Misawa S,Ohsaka A,Takahashi K

    更新日期：2020-10-19 00:00:00

  • Genomic characterization of plant cell wall degrading enzymes and in silico analysis of xylanases and polygalacturonases of Fusarium virguliforme.

    abstract：BACKGROUND:Plant cell wall degrading enzymes (PCWDEs) are a subset of carbohydrate-active enzymes (CAZy) produced by plant pathogens to degrade plant cell walls. To counteract PCWDEs, plants release PCWDEs inhibitor proteins (PIPs) to reduce their impact. Several transgenic plants expressing exogenous PIPs that interac...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/s12866-016-0761-0

    authors： Chang HX,Yendrek CR,Caetano-Anolles G,Hartman GL

    更新日期：2016-07-12 00:00:00

  • Bactericidal activity of M protein conserved region antibodies against group A streptococcal isolates from the Northern Thai population.

    abstract：BACKGROUND:Most group A streptococcal (GAS) vaccine strategies have focused on the surface M protein, a major virulence factor of GAS. The amino-terminus of the M protein elicits antibodies, that are both opsonic and protective, but which are type specific. J14, a chimeric peptide that contains 14 amino acids from the ...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/1471-2180-6-71

    authors： Yoonim N,Olive C,Pruksachatkunakorn C,Pruksakorn S

    更新日期：2006-08-09 00:00:00

  • Habitat disturbance influences the skin microbiome of a rediscovered neotropical-montane frog.

    abstract：BACKGROUND:The skin microbiome serves as a first line defense against pathogens in vertebrates. In amphibians, it has the potential to protect against the chytrid fungus Batrachochytrium dendrobatis (Bd), a likely agent of amphibian declines. Alteration of the microbiome associated with unfavorable environmental change...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/s12866-020-01979-1

    authors： Jiménez RR,Alvarado G,Sandoval J,Sommer S

    更新日期：2020-09-22 00:00:00

  • Characterization of biofilm matrix, degradation by DNase treatment and evidence of capsule downregulation in Streptococcus pneumoniae clinical isolates.

    abstract：BACKGROUND:Streptococcus pneumoniae is a common respiratory pathogen and a major causative agent of respiratory infections, including otitis media (OM). Pneumococcal biofilms have been demonstrated on biopsies of the middle ear mucosa in children receiving tympanostomy tubes, supporting the hypothesis that chronic OM m...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/1471-2180-8-173

    authors： Hall-Stoodley L,Nistico L,Sambanthamoorthy K,Dice B,Nguyen D,Mershon WJ,Johnson C,Hu FZ,Stoodley P,Ehrlich GD,Post JC

    更新日期：2008-10-08 00:00:00

  • Purification and characterization of a surfactin-like molecule produced by Bacillus sp. H2O-1 and its antagonistic effect against sulfate reducing bacteria.

    abstract：BACKGROUND:Bacillus sp. H2O-1, isolated from the connate water of a Brazilian reservoir, produces an antimicrobial substance (denoted as AMS H2O-1) that is active against sulfate reducing bacteria, which are the major bacterial group responsible for biogenic souring and biocorrosion in petroleum reservoirs. Thus, the u...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/1471-2180-12-252

    authors： Korenblum E,de Araujo LV,Guimarães CR,de Souza LM,Sassaki G,Abreu F,Nitschke M,Lins U,Freire DM,Barreto-Bergter E,Seldin L

    更新日期：2012-11-07 00:00:00

  • Modified high-throughput Nile red fluorescence assay for the rapid screening of oleaginous yeasts using acetic acid as carbon source.

    abstract：BACKGROUND:Over the last years oleaginous yeasts have been studied for several energetic, oleochemical, medical and pharmaceutical purposes. However, only a small number of yeasts are known and have been deeply exploited. The search for new isolates with high oleaginous capacity becomes imperative, as well as the use o...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/s12866-020-01742-6

    authors： Miranda C,Bettencourt S,Pozdniakova T,Pereira J,Sampaio P,Franco-Duarte R,Pais C

    更新日期：2020-03-14 00:00:00

  • Involvement of TIP60 acetyltransferase in intracellular Salmonella replication.

    abstract：BACKGROUND:Salmonella enterica is a facultative intracellular pathogen that replicates within a membrane-bound compartment termed Salmonella containing vacuole (SCV). The biogenesis of SCV requires Salmonella type III protein secretion/translocation system and their effector proteins which are translocated into host ce...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/1471-2180-10-228

    authors： Wang X,Li D,Qu D,Zhou D

    更新日期：2010-08-26 00:00:00

  • Identification of a bacteriocin and its cognate immunity factor expressed by Moraxella catarrhalis.

    abstract：BACKGROUND:Bacteriocins are antimicrobial proteins and peptides ribosomally synthesized by some bacteria which can effect both intraspecies and interspecies killing. RESULTS:Moraxella catarrhalis strain E22 containing plasmid pLQ510 was shown to inhibit the growth of M. catarrhalis strain O35E. Two genes (mcbA and mcb...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/1471-2180-9-207

    authors： Attia AS,Sedillo JL,Hoopman TC,Liu W,Liu L,Brautigam CA,Hansen EJ

    更新日期：2009-09-25 00:00:00

  • Structure, Genetics and Worldwide Spread of New Delhi Metallo-β-lactamase (NDM): a threat to public health.

    abstract：BACKGROUND:The emergence of carbapenemase producing bacteria, especially New Delhi metallo-β-lactamase (NDM-1) and its variants, worldwide, has raised amajor public health concern. NDM-1 hydrolyzes a wide range of β-lactam antibiotics, including carbapenems, which are the last resort of antibiotics for the treatment of...

    journal_title：BMC microbiology

    pub_type： 杂志文章,评审

    doi：10.1186/s12866-017-1012-8

    authors： Khan AU,Maryam L,Zarrilli R

    更新日期：2017-04-27 00:00:00

  • Correction to: Identification of cholesterol-assimilating actinomycetes strain and application of statistical modeling approaches for improvement of cholesterol oxidase production by Streptomyces anulatus strain NEAE-94.

    abstract：:Following publication of the original article [1], the authors reported an error in Fig. 2c. ...

    journal_title：BMC microbiology

    pub_type： 已发布勘误

    doi：10.1186/s12866-020-01791-x

    authors： El-Naggar NE,El-Shweihy NM

    更新日期：2020-04-30 00:00:00

  • Expression and subcellular localization of kinetoplast-associated proteins in the different developmental stages of Trypanosoma cruzi.

    abstract：BACKGROUND:The kinetoplast DNA (kDNA) of trypanosomatids consists of an unusual arrangement of circular molecules catenated into a single network. The diameter of the isolated kDNA network is similar to that of the entire cell. However, within the kinetoplast matrix, the kDNA is highly condensed. Studies in Crithidia f...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/1471-2180-9-120

    authors： Cavalcanti DP,Shimada MK,Probst CM,Souto-Padrón TC,de Souza W,Goldenberg S,Fragoso SP,Motta MC

    更新日期：2009-06-04 00:00:00

  • Special issue on enhancing vector refractoriness to trypanosome infection-foreword.

    abstract：: ...

    journal_title：BMC microbiology

    pub_type：

    doi：10.1186/s12866-018-1279-4

    authors： Abd-Alla AMM,Tsiamis G,Boucias DG

    更新日期：2018-11-23 00:00:00

  • Genetic and pathogenic difference between Streptococcus agalactiae serotype Ia fish and human isolates.

    abstract：BACKGROUND:Streptococcus agalactiae (GBS) is a common pathogen to infect newborn, woman, the elderly, and immuno-compromised human and fish. 37 fish isolates and 554 human isolates of the GBS in 2007-2012 were investigated in serotypes, antibiotic susceptibility, genetic difference and pathogenicity to tilapia. RESULT...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/s12866-016-0794-4

    authors： Chu C,Huang PY,Chen HM,Wang YH,Tsai IA,Lu CC,Chen CC

    更新日期：2016-08-02 00:00:00

  • Extracellular DNA release confers heterogeneity in Candida albicans biofilm formation.

    abstract：BACKGROUND:Biofilm formation by Candida albicans has shown to be highly variable and is directly associated with pathogenicity and poor clinical outcomes in patients at risk. The aim of this study was to test the hypotheses that the extracellular DNA release by C. albicans is strain dependent and is associated with bio...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/s12866-014-0303-6

    authors： Rajendran R,Sherry L,Lappin DF,Nile CJ,Smith K,Williams C,Munro CA,Ramage G

    更新日期：2014-12-05 00:00:00

  • First isolation and whole-genome sequencing of a Shewanella algae strain from a swine farm in Brazil.

    abstract：BACKGROUND:Infections caused by Shewanella spp. have been increasingly reported worldwide. The advances in genomic sciences have enabled better understanding about the taxonomy and epidemiology of this agent. However, the scarcity of DNA sequencing data is still an obstacle for understanding the genus and its associati...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/s12866-020-02040-x

    authors： Alves VBA,Carvalho E,Madureira PA,Marino ED,Vaz ACN,Vidal AMC,Ruiz VLA

    更新日期：2020-11-24 00:00:00

  • Pyrosequencing analysis revealed complex endogenetic microorganism community from natural DongChong XiaCao and its microhabitat.

    abstract：BACKGROUND:Ophiocordyceps sinensis (DongChong XiaCao (DCXC) in Chinese), a fungal parasite of caterpillars, is a traditional Chinese medicine. Bioactive components isolated from natural DCXC possess a wide range of pharmacological actions. Many efforts have been directed towards isolating the fungi based on culture-dep...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/s12866-016-0813-5

    authors： Xia F,Liu Y,Guo MY,Shen GR,Lin J,Zhou XW

    更新日期：2016-08-26 00:00:00

  • A microbiome case-control study of recurrent acute otitis media identified potentially protective bacterial genera.

    abstract：BACKGROUND:Recurrent acute otitis media (rAOM, recurrent ear infection) is a common childhood disease caused by bacteria termed otopathogens, for which current treatments have limited effectiveness. Generic probiotic therapies have shown promise, but seem to lack specificity. We hypothesised that healthy children with ...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/s12866-018-1154-3

    authors： Lappan R,Imbrogno K,Sikazwe C,Anderson D,Mok D,Coates H,Vijayasekaran S,Bumbak P,Blyth CC,Jamieson SE,Peacock CS

    更新日期：2018-02-20 00:00:00

  • Laboratory contamination in airway microbiome studies.

    abstract：BACKGROUND:The low bacterial load in samples acquired from the lungs, have made studies on the airway microbiome vulnerable to contamination from bacterial DNA introduced during sampling and laboratory processing. We have examined the impact of laboratory contamination on samples collected from the lower airways by pro...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/s12866-019-1560-1

    authors： Drengenes C,Wiker HG,Kalananthan T,Nordeide E,Eagan TML,Nielsen R

    更新日期：2019-08-14 00:00:00

  • Comparative analysis of diguanylate cyclase and phosphodiesterase genes in Klebsiella pneumoniae.

    abstract：BACKGROUND:Klebsiella pneumoniae can be found in environmental habitats as well as in hospital settings where it is commonly associated with nosocomial infections. One of the factors that contribute to virulence is its capacity to form biofilms on diverse biotic and abiotic surfaces. The second messenger Bis-(3'-5')-cy...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/1471-2180-12-139

    authors： Cruz DP,Huertas MG,Lozano M,Zárate L,Zambrano MM

    更新日期：2012-07-09 00:00:00

  • A proteomic study of Corynebacterium glutamicum AAA+ protease FtsH.

    abstract：BACKGROUND:The influence of the membrane-bound AAA+ protease FtsH on membrane and cytoplasmic proteins of Corynebacterium glutamicum was investigated in this study. For the analysis of the membrane fraction, anion exchange chromatography was combined with SDS-PAGE, while the cytoplasmic protein fraction was studied by ...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/1471-2180-7-6

    authors： Lüdke A,Krämer R,Burkovski A,Schluesener D,Poetsch A

    更新日期：2007-01-25 00:00:00

  • Transcriptional analysis of the Escherichia coli ColV-Ia plasmid pS88 during growth in human serum and urine.

    abstract：BACKGROUND:The sequenced O45:K1:H7 Escherichia coli meningitis strain S88 harbors a large virulence plasmid. To identify possible genetic determinants of pS88 virulence, we examined the transcriptomes of 88 plasmidic ORFs corresponding to known and putative virulence genes, and 35 ORFs of unknown function. RESULTS:Qua...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/1471-2180-12-115

    authors： Lemaître C,Bidet P,Bingen E,Bonacorsi S

    更新日期：2012-06-21 00:00:00

  • Analysis of stress- and host cell-induced expression of the Mycobacterium tuberculosis inorganic pyrophosphatase.

    abstract：BACKGROUND:The gene encoding the inorganic pyrophosphatase (PPase) of the intracellular pathogen Legionella pneumophila is induced during intracellular infection, but is constitutively expressed in Escherichia coli. The causative agent of tuberculosis, Mycobacterium tuberculosis, contains a well conserved copy of PPase...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/1471-2180-1-3

    authors： Triccas JA,Gicquel B

    更新日期：2001-01-01 00:00:00

  • The influence of acetyl phosphate on DspA signalling in the Cyanobacterium Synechocystis sp. PCC6803.

    abstract：BACKGROUND:The dspA (hik33) gene, coding for a putative sensory histidine kinase, is conserved in plastids (ycf26) and cyanobacteria. It has been linked with a number of different stress responses in cyanobacteria. RESULTS:We constructed an insertional mutant of dspA (ycf26) in Synechocystis 6803. We found little phen...

    journal_title：BMC microbiology

    pub_type： 杂志文章

    doi：10.1186/1471-2180-5-47

    authors： Morrison SS,Mullineaux CW,Ashby MK

    更新日期：2005-08-02 00:00:00