Structure, Genetics and Worldwide Spread of New Delhi Metallo-β-lactamase (NDM): a threat to public health.


BACKGROUND:The emergence of carbapenemase producing bacteria, especially New Delhi metallo-β-lactamase (NDM-1) and its variants, worldwide, has raised amajor public health concern. NDM-1 hydrolyzes a wide range of β-lactam antibiotics, including carbapenems, which are the last resort of antibiotics for the treatment of infections caused by resistant strain of bacteria. MAIN BODY:In this review, we have discussed bla NDM-1variants, its genetic analysis including type of specific mutation, origin of country and spread among several type of bacterial species. Wide members of enterobacteriaceae, most commonly Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, and gram-negative non-fermenters Pseudomonas spp. and Acinetobacter baumannii were found to carry these markers. Moreover, at least seventeen variants of bla NDM-type gene differing into one or two residues of amino acids at distinct positions have been reported so far among different species of bacteria from different countries. The genetic and structural studies of these variants are important to understand the mechanism of antibiotic hydrolysis as well as to design new molecules with inhibitory activity against antibiotics. CONCLUSION:This review provides a comprehensive view of structural differences among NDM-1 variants, which are a driving force behind their spread across the globe.


BMC Microbiol


BMC microbiology


Khan AU,Maryam L,Zarrilli R




Has Abstract


2017-04-27 00:00:00












  • Duodenal and faecal microbiota of celiac children: molecular, phenotype and metabolome characterization.

    abstract:BACKGROUND:Epidemiology of celiac disease (CD) is increasing. CD mainly presents in early childhood with small intestinal villous atrophy and signs of malabsorption. Compared to healthy individuals, CD patients seemed to be characterized by higher numbers of Gram-negative bacteria and lower numbers Gram-positive bacter...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Di Cagno R,De Angelis M,De Pasquale I,Ndagijimana M,Vernocchi P,Ricciuti P,Gagliardi F,Laghi L,Crecchio C,Guerzoni ME,Gobbetti M,Francavilla R

    更新日期:2011-10-04 00:00:00

  • Genetic stability of Brucella abortus isolates from an outbreak by multiple-locus variable-number tandem repeat analysis (MLVA16).

    abstract:BACKGROUND:Brucellosis caused by Brucella abortus is one of the most important zoonoses in the world. Multiple-locus variable-number tandem repeat analysis (MLVA16) has been shown be a useful tool to epidemiological traceback studies in B. abortus infection. Thus, the present study aimed (i) to evaluate the genetic div...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Dorneles EM,Santana JA,Alves TM,Pauletti RB,Mol JP,Heinemann MB,Lage AP

    更新日期:2014-07-11 00:00:00

  • Mycobacterium tuberculosis interactome analysis unravels potential pathways to drug resistance.

    abstract:BACKGROUND:Emergence of drug resistant varieties of tuberculosis is posing a major threat to global tuberculosis eradication programmes. Although several approaches have been explored to counter resistance, there has been limited success due to a lack of understanding of how resistance emerges in bacteria upon drug tre...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Raman K,Chandra N

    更新日期:2008-12-23 00:00:00

  • Long-term impact of fecal transplantation in healthy volunteers.

    abstract:BACKGROUND:Fecal microbiota transplantation (FMT) has been recently approved by FDA for the treatment of refractory recurrent clostridial colitis (rCDI). Success of FTM in treatment of rCDI led to a number of studies investigating the effectiveness of its application in the other gastrointestinal diseases. However, in ...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Goloshchapov OV,Olekhnovich EI,Sidorenko SV,Moiseev IS,Kucher MA,Fedorov DE,Pavlenko AV,Manolov AI,Gostev VV,Veselovsky VA,Klimina KM,Kostryukova ES,Bakin EA,Shvetcov AN,Gumbatova ED,Klementeva RV,Shcherbakov AA,Gorchak

    更新日期:2019-12-30 00:00:00

  • Characterization of alpha-isopropylmalate synthases containing different copy numbers of tandem repeats in Mycobacterium tuberculosis.

    abstract:BACKGROUND:Alpha-isopropylmalate synthase (alpha-IPMS) is the key enzyme that catalyzes the first committed step in the leucine biosynthetic pathway. The gene encoding alpha-IPMS in Mycobacterium tuberculosis, leuA, is polymorphic due to the insertion of 57-bp repeat units referred to as Variable Number of Tandem Repea...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Yindeeyoungyeon W,Likitvivatanavong S,Palittapongarnpim P

    更新日期:2009-06-09 00:00:00

  • Escherichia coli phylogenetic group determination and its application in the identification of the major animal source of fecal contamination.

    abstract:BACKGROUND:Escherichia coli strains are commonly found in the gut microflora of warm-blooded animals. These strains can be assigned to one of the four main phylogenetic groups, A, B1, B2 and D, which can be divided into seven subgroups (A0, A1, B1, B22, B23, D1 and D2), according to the combination of the three genetic...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Carlos C,Pires MM,Stoppe NC,Hachich EM,Sato MI,Gomes TA,Amaral LA,Ottoboni LM

    更新日期:2010-06-01 00:00:00

  • The role of the two-component systems Cpx and Arc in protein alterations upon gentamicin treatment in Escherichia coli.

    abstract:BACKGROUND:The aminoglycoside antibiotic gentamicin was supposed to induce a crosstalk between the Cpx- and the Arc-two-component systems (TCS). Here, we investigated the physical interaction of the respective TCS components and compared the results with their respective gene expression and protein abundance. The findi...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Ćudić E,Surmann K,Panasia G,Hammer E,Hunke S

    更新日期:2017-09-18 00:00:00

  • Genetic diversity and characterization of arsenic-resistant endophytic bacteria isolated from Pteris vittata, an arsenic hyperaccumulator.

    abstract:BACKGROUND:Alleviating arsenic (As) contamination is a high-priority environmental issue. Hyperaccumulator plants may harbor endophytic bacteria able to detoxify As. Therefore, we investigated the distribution, diversity, As (III) resistance levels, and resistance-related functional genes of arsenite-resistant bacteria...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Gu Y,Wang Y,Sun Y,Zhao K,Xiang Q,Yu X,Zhang X,Chen Q

    更新日期:2018-05-08 00:00:00

  • Transcriptomic and proteomic analyses of core metabolism in Clostridium termitidis CT1112 during growth on α-cellulose, xylan, cellobiose and xylose.

    abstract:BACKGROUND:Clostridium termitidis CT1112 is an anaerobic, Gram-positive, mesophilic, spore-forming, cellulolytic bacterium, originally isolated from the gut of a wood feeding termite Nasusitermes lujae. It has the ability to hydrolyze both cellulose and hemicellulose, and ferment the degradation products to acetate, fo...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Munir RI,Spicer V,Krokhin OV,Shamshurin D,Zhang X,Taillefer M,Blunt W,Cicek N,Sparling R,Levin DB

    更新日期:2016-05-23 00:00:00

  • Application of hierarchical oligonucleotide primer extension (HOPE) to assess relative abundances of ammonia- and nitrite-oxidizing bacteria.

    abstract:BACKGROUND:Establishing an optimal proportion of nitrifying microbial populations, including ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB), complete nitrite oxidizers (comammox) and ammonia-oxidizing archaea (AOA), is important for ensuring the efficiency of nitrification in water treatment systems...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Scarascia G,Cheng H,Harb M,Hong PY

    更新日期:2017-04-04 00:00:00

  • The HMI™ module: a new tool to study the Host-Microbiota Interaction in the human gastrointestinal tract in vitro.

    abstract:BACKGROUND:Recent scientific developments have shed more light on the importance of the host-microbe interaction, particularly in the gut. However, the mechanistic study of the host-microbe interplay is complicated by the intrinsic limitations in reaching the different areas of the gastrointestinal tract (GIT) in vivo....

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Marzorati M,Vanhoecke B,De Ryck T,Sadaghian Sadabad M,Pinheiro I,Possemiers S,Van den Abbeele P,Derycke L,Bracke M,Pieters J,Hennebel T,Harmsen HJ,Verstraete W,Van de Wiele T

    更新日期:2014-05-22 00:00:00

  • The prevalence of gene duplications and their ancient origin in Rhodobacter sphaeroides 2.4.1.

    abstract:BACKGROUND:Rhodobacter sphaeroides 2.4.1 is a metabolically versatile organism that belongs to α-3 subdivision of Proteobacteria. The present study was to identify the extent, history, and role of gene duplications in R. sphaeroides 2.4.1, an organism that possesses two chromosomes. RESULTS:A protein similarity search...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Bavishi A,Lin L,Schroeder K,Peters A,Cho H,Choudhary M

    更新日期:2010-12-30 00:00:00

  • Three redescriptions in Tintinnopsis (Protista: Ciliophora: Tintinnina) from coastal waters of China, with cytology and phylogenetic analyses based on ribosomal RNA genes.

    abstract:BACKGROUND:The taxonomy of tintinnine ciliates is vastly unresolved because it has traditionally been based on the lorica (a secreted shell) and it has only recently incorporated cytological and molecular information. Tintinnopsis, the most speciose tintinnine genus, is also the most problematic: it is known to be non-...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Bai Y,Wang R,Song W,Li L,Santoferrara LF,Hu X

    更新日期:2020-12-14 00:00:00

  • Bacillus anthracis S-layer protein BslA binds to extracellular matrix by interacting with laminin.

    abstract:BACKGROUND:The Bacillus anthracis S-layer protein, BslA, plays a crucial role in mammalian infection. BslA is required to mediate adherence between host cells and vegetative forms of bacteria and this interaction promotes target organs adherence and blood-brain barrier (BBB) penetration in vivo. This study attempts to ...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Wang Y,Wei Y,Yuan S,Tao H,Dong J,Zhang Z,Tian W,Liu C

    更新日期:2016-08-11 00:00:00

  • Diversity of Mycobacterium tuberculosis and drug resistance in different provinces of Papua New Guinea.

    abstract:BACKGROUND:Papua New Guinea (PNG) is a high tuberculosis (TB) burden country of the WHO Western Pacific Region, but so far research on drug resistance (DR) and genotypes of Mycobacterium tuberculosis (M. tuberculosis) was only conducted in few provinces in the country. The aim of the present study was to obtain baselin...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Ley SD,Harino P,Vanuga K,Kamus R,Carter R,Coulter C,Pandey S,Feldmann J,Ballif M,Siba PM,Phuanukoonnon S,Gagneux S,Beck HP

    更新日期:2014-12-05 00:00:00

  • A recombinase system facilitates cloning of expression cassettes in the ciliate Tetrahymena thermophila.

    abstract:BACKGROUND:Tetrahymena thermophila is one of the best characterized unicellular eukaryotes and its genome is sequenced in its entirety. However, the AT-richness of the genome and an unusual codon usage cause problems in cloning and expression of the ciliate DNA. To overcome these technical hiatuses we developed a Cre-d...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Weide T,Bockau U,Rave A,Herrmann L,Hartmann MW

    更新日期:2007-03-01 00:00:00

  • Outer membrane protein P4 is not required for virulence in the human challenge model of Haemophilus ducreyi infection.

    abstract:BACKGROUND:Bacterial lipoproteins often play important roles in pathogenesis and can stimulate protective immune responses. Such lipoproteins are viable vaccine candidates. Haemophilus ducreyi, which causes the sexually transmitted disease chancroid, expresses a number of lipoproteins during human infection. One such l...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Janowicz DM,Zwickl BW,Fortney KR,Katz BP,Bauer ME

    更新日期:2014-06-24 00:00:00

  • Identification of DNA sequence variation in Campylobacter jejuni strains associated with the Guillain-Barré syndrome by high-throughput AFLP analysis.

    abstract:BACKGROUND:Campylobacter jejuni is the predominant cause of antecedent infection in post-infectious neuropathies such as the Guillain-Barré (GBS) and Miller Fisher syndromes (MFS). GBS and MFS are probably induced by molecular mimicry between human gangliosides and bacterial lipo-oligosaccharides (LOS). This study desc...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Godschalk PC,Bergman MP,Gorkink RF,Simons G,van den Braak N,Lastovica AJ,Endtz HP,Verbrugh HA,van Belkum A

    更新日期:2006-04-04 00:00:00

  • Whole-genome sequence-based comparison and profiling of virulence-associated genes of Bacillus cereus group isolates from diverse sources in Japan.

    abstract:BACKGROUND:The complete genome sequences of 44 Bacillus cereus group isolates collected from diverse sources in Japan were analyzed to determine their genetic backgrounds and diversity levels in Japan. Multilocus sequence typing (MLST) and core-genome single-nucleotide polymorphism (SNP) typing data from whole-genome s...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Okutani A,Inoue S,Noguchi A,Kaku Y,Morikawa S

    更新日期:2019-12-16 00:00:00

  • Candida krusei form mycelia along agar surfaces towards each other and other Candida species.

    abstract:BACKGROUND:Candida krusei has been known to exhibit communal interactions such as pellicle formation and crawling out of nutritional broth. We noticed another possible interaction on agar surfaces, where C. krusei yeast cells formed mycelia along agar surfaces toward each other. We report here the results of experiment...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Fleischmann J,Broeckling CD,Lyons S

    更新日期:2017-03-11 00:00:00

  • The role of iron uptake in pathogenicity and symbiosis in Photorhabdus luminescens TT01.

    abstract:BACKGROUND:Photorhabdus are Gram negative bacteria that are pathogenic to insect larvae whilst also having a mutualistic interaction with nematodes from the family Heterorhabditis. Iron is an essential nutrient and bacteria have different mechanisms for obtaining both the ferrous (Fe2+) and ferric (Fe3+) forms of this ...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Watson RJ,Millichap P,Joyce SA,Reynolds S,Clarke DJ

    更新日期:2010-06-22 00:00:00

  • Comparative gene expression analysis of planktonic Porphyromonas gingivalis ATCC 33277 in the presence of a growing biofilm versus planktonic cells.

    abstract:BACKGROUND:Porphyromonas gingivalis, a microorganism residing in the oral cavity within complex multispecies biofilms, is one of the keystone pathogens in the onset and progression of periodontitis. In this in vitro study, using DNA microarray, we investigate the differential gene expression of Porphyromonas gingivalis...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Sánchez MC,Romero-Lastra P,Ribeiro-Vidal H,Llama-Palacios A,Figuero E,Herrera D,Sanz M

    更新日期:2019-03-12 00:00:00

  • Pseudoscorpion Wolbachia symbionts: diversity and evidence for a new supergroup S.

    abstract:BACKGROUND:Wolbachia are the most widely spread endosymbiotic bacteria, present in a wide variety of insects and two families of nematodes. As of now, however, relatively little genomic data has been available. The Wolbachia symbiont can be parasitic, as described for many arthropod systems, an obligate mutualist, as i...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Lefoulon E,Clark T,Borveto F,Perriat-Sanguinet M,Moulia C,Slatko BE,Gavotte L

    更新日期:2020-06-30 00:00:00

  • The microbiota in the intestinal and respiratory tracts of naked mole-rats revealed by high-throughput sequencing.

    abstract:BACKGROUND:The naked mole-rat (NMR, Heterocephalus glaber) is being bred as a novel laboratory animal due to its unique biological characteristics, including longevity, cancer resistance, hypoxia tolerance, and pain insensitivity. It is expected that differences exist between the microbiota of wild NMRs and that of NMR...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Cong W,Xing J,Feng Y,Wang J,Fu R,Yue B,He Z,Lin L,Yang W,Cheng J,Sun W,Cui S

    更新日期:2018-08-22 00:00:00

  • Identification and characterization of intervening sequences within 23S rRNA genes from more than 200 Campylobacter isolates from seven species including atypical campylobacters.

    abstract:BACKGROUND:Identification and characterization of intervening sequences (IVSs) within 23S rRNA genes from Campylobacter organisms including atypical campylobacters were carried out using two PCR primer pairs, designed to generate helix 25 and 45 regions. RESULTS:Only C. sputorum biovar sputorum LMG7975 and fecalis LMG...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Tazumi A,Kakinuma Y,Misawa N,Moore JE,Millar BC,Matsuda M

    更新日期:2009-12-11 00:00:00

  • Toxigenic potential and antimicrobial susceptibility of Bacillus cereus group bacteria isolated from Tunisian foodstuffs.

    abstract:BACKGROUND:Despite the importance of the B. cereus group as major foodborne pathogens that may cause diarrheal and/or emetic syndrome(s), no study in Tunisia has been conducted in order to characterize the pathogenic potential of the B. cereus group. The aim of this study was to assess the sanitary potential risks of 1...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Gdoura-Ben Amor M,Jan S,Baron F,Grosset N,Culot A,Gdoura R,Gautier M,Techer C

    更新日期:2019-08-24 00:00:00

  • Environmental stresses inhibit splicing in the aquatic fungus Blastocladiella emersonii.

    abstract:BACKGROUND:Exposure of cells to environmental stress conditions can lead to the interruption of several intracellular processes, in particular those performed by macromolecular complexes such as the spliceosome. RESULTS:During nucleotide sequencing of cDNA libraries constructed using RNA isolated from B. emersonii cel...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Georg RC,Stefani RM,Gomes SL

    更新日期:2009-10-29 00:00:00

  • CCL5 regulation of mucosal chlamydial immunity and infection.

    abstract:BACKGROUND:Following genital chlamydial infection, an early T helper type 1 (Th1)-associated immune response precedes the activation and recruitment of specific Th1 cells bearing distinct chemokine receptors, subsequently leading to the clearance of Chlamydia. We have shown that CCR5, a receptor for CCL5, is crucial fo...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Sakthivel SK,Singh UP,Singh S,Taub DD,Igietseme JU,Lillard JW Jr

    更新日期:2008-08-13 00:00:00

  • Small molecule inhibitors of the Yersinia type III secretion system impair the development of Chlamydia after entry into host cells.

    abstract:BACKGROUND:Chlamydiae are obligate intracellular pathogens that possess a type III secretion system to deliver proteins into the host cell during infection. Small molecule inhibitors of type III secretion in Yersinia, termed INPs (Innate Pharmaceuticals AB) were reported to strongly inhibit Chlamydia growth in epitheli...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Muschiol S,Normark S,Henriques-Normark B,Subtil A

    更新日期:2009-04-21 00:00:00

  • Comparative genomics of Enterococcus spp. isolated from bovine feces.

    abstract:BACKGROUND:Enterococcus is ubiquitous in nature and is a commensal of both the bovine and human gastrointestinal (GI) tract. It is also associated with clinical infections in humans. Subtherapeutic administration of antibiotics to cattle selects for antibiotic resistant enterococci in the bovine GI tract. Antibiotic re...

    journal_title:BMC microbiology

    pub_type: 杂志文章


    authors: Beukers AG,Zaheer R,Goji N,Amoako KK,Chaves AV,Ward MP,McAllister TA

    更新日期:2017-03-08 00:00:00