Abstract:
BACKGROUND:Bartonella henselae is a Gram-negative bacterium transmitted to humans by a scratch from cat in the presence of ectoparasites. Humans infected with B. henselae can result in various clinical diseases including local lymphadenopathy and more serious systemic disease such as persistent bacteremia and endocarditis. The current treatment of persistent B. henselae infections is not very effective and remains a challenge. To find more effective treatments for persistent and biofilm Bartonella infections, in this study, we evaluated a panel of drugs and drug combinations based on the current treatment and also promising hits identified from a recent drug screen against stationary phase and biofilm recovered cells of B. henselae. RESULTS:We evaluated 14 antibiotics and 25 antibiotic combinations for activity against stationary phase B. henselae (all antibiotics were at 5 μg/ml) and found that ciprofloxacin, gentamicin, and nitrofurantoin were the most active agents, while clofazimine and miconazole had poor activity. Drug combinations azithromycin/ciprofloxacin, azithromycin/methylene blue, rifampin/ciprofloxacin, and rifampin/methylene blue could rapidly kill stationary phase B. henselae with no detectable CFU after 1-day exposure. Methylene blue and rifampin were the most active agents against the biofilm B. henselae after 6 days of drug exposure. Antibiotic combinations (azithromycin/ciprofloxacin, azithromycin/methylene blue, rifampin/ciprofloxacin, rifampin/methylene blue) completely eradicated the biofilm B. henselae after treatment for 6 days. CONCLUSIONS:These findings may facilitate development of more effective treatment of persistent Bartonella infections in the future.
journal_name
BMC Microbioljournal_title
BMC microbiologyauthors
Zheng X,Ma X,Li T,Shi W,Zhang Ydoi
10.1186/s12866-020-01777-9subject
Has Abstractpub_date
2020-04-10 00:00:00pages
87issue
1issn
1471-2180pii
10.1186/s12866-020-01777-9journal_volume
20pub_type
杂志文章相关文献
BMC MICROBIOLOGY文献大全abstract:: ...
journal_title:BMC microbiology
pub_type:
doi:10.1186/s12866-018-1279-4
更新日期:2018-11-23 00:00:00
abstract:BACKGROUND:The skin microbiome serves as a first line defense against pathogens in vertebrates. In amphibians, it has the potential to protect against the chytrid fungus Batrachochytrium dendrobatis (Bd), a likely agent of amphibian declines. Alteration of the microbiome associated with unfavorable environmental change...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01979-1
更新日期:2020-09-22 00:00:00
abstract:BACKGROUND:The evolution of mycoplasmas from a common ancestor with Firmicutes has been characterized not only by genome down-sizing but also by horizontal gene transfer between mycoplasma species sharing a common host. The mechanisms of these gene transfers remain unclear because our knowledge of the mycoplasma mobile...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-257
更新日期:2012-11-12 00:00:00
abstract:BACKGROUND:Fusobacterium nucleatum, a well studied bacterium in periodontal diseases, appendicitis, gingivitis, osteomyelitis and pregnancy complications has recently gained attention due to its association with colorectal cancer (CRC) progression. Treatment with berberine was shown to reverse F. nucleatum-induced CRC ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0700-0
更新日期:2016-05-12 00:00:00
abstract:BACKGROUND:The catabolite control protein CcpA is a transcriptional regulator conserved in many Gram-positives, controlling the efficiency of glucose metabolism. Here we studied the role of Bacillus cereus ATCC 14579 CcpA in regulation of metabolic pathways and expression of enterotoxin genes by comparative transcripto...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-62
更新日期:2008-04-16 00:00:00
abstract:BACKGROUND:The Rio Grande River is the natural boundary between U.S. and Mexico from El Paso, TX to Brownsville, TX. and is one of the major water resources of the area. Agriculture, farming, maquiladora industry, domestic activities, as well as differences in disposal regulations and enforcement increase the contamina...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-4-17
更新日期:2004-04-22 00:00:00
abstract:BACKGROUND:Emergence of drug resistant varieties of tuberculosis is posing a major threat to global tuberculosis eradication programmes. Although several approaches have been explored to counter resistance, there has been limited success due to a lack of understanding of how resistance emerges in bacteria upon drug tre...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-234
更新日期:2008-12-23 00:00:00
abstract:BACKGROUND:Salmonella enterica serotype Enteritidis (S. Enteritidis) is a zoonotic pathogen, which can be found in many sources including animals and the environment. However, little is known about the molecular relatedness among S. Enteritidis isolates from different sources. We have applied multiple-locus variable nu...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-146
更新日期:2008-09-15 00:00:00
abstract:BACKGROUND:Mycoplasma pneumoniae has previously been characterized as a micro-organism that is genetically highly stable. In spite of this genetic stability, homologous DNA recombination has been hypothesized to lie at the basis of antigenic variation of the major surface protein, P1, of M. pneumoniae. In order to iden...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-167
更新日期:2008-10-02 00:00:00
abstract:BACKGROUND:Acetic acid has been used to clean and disinfect surfaces in the household for many decades. The antimicrobial efficacy of cleaning procedures can be considered particularly important for young, old, pregnant, immunocompromised people, but may also concern other groups, particularly with regards to the COVID...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01948-8
更新日期:2020-08-26 00:00:00
abstract:BACKGROUND:It is unclear how DNA is packaged in a bacterial cell in the absence of nucleosomes. To investigate the initial level of DNA condensation in bacterial nucleoid we used in vivo DNA digestion coupled with high-throughput sequencing of the digestion-resistant fragments. To this end, we transformed E. coli cells...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0637-3
更新日期:2016-02-20 00:00:00
abstract:BACKGROUND:L-arabitol dehydrogenase (LAD) and xylitol dehydrogenase (XDH) are involved in the degradation of L-arabinose and D-xylose, which are among the most abundant monosaccharides on earth. Previous data demonstrated that LAD and XDH not only differ in the activity on their biological substrate, but also that only...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-166
更新日期:2009-08-12 00:00:00
abstract:BACKGROUND:The increasing availability of whole genome sequences allows the gene or protein content of different organisms to be compared, leading to burgeoning interest in the relatively new subfield of pan-genomics. However, while several studies have analyzed protein content relationships in specific groups of bacte...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-258
更新日期:2010-10-13 00:00:00
abstract:BACKGROUND:Streptococcus suis is a major swine pathogen worldwide that causes meningitis, septicemia, arthritis, and endocarditis. Using animal models, a surface-associated subtilisin-like protease (SspA) has recently been shown to be an important virulence factor for S. suis. In this study, we hypothesized that the S....
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-11-47
更新日期:2011-03-01 00:00:00
abstract:BACKGROUND:The species Pectobacterium carotovorum includes a diverse subspecies of bacteria that cause disease on a wide variety of plants. In Morocco, approximately 95% of the P. carotovorum isolates from potato plants with tuber soft rot are P. carotovorum subsp. carotovorum. However, identification of this pathogen ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-176
更新日期:2013-07-29 00:00:00
abstract:BACKGROUND:Sinorhizobium meliloti is an agriculturally important model symbiont. There is an ongoing need to update and improve its genome annotation. In this study, we used a high-throughput pyrosequencing approach to sequence the transcriptome of S. meliloti, and search for new bacterial genes missed in the previous ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-72
更新日期:2008-05-02 00:00:00
abstract:BACKGROUND:Enterohemorrhagic Escherichia coli (EHEC) colonizes the intestinal epithelium and causes attaching and effacing (A/E) lesions. Expression of virulence genes, particularly those from the locus of the enterocyte effacement (LEE) pathogenicity island is required for the formation of a type three secretion syste...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-231
更新日期:2012-10-11 00:00:00
abstract:BACKGROUND:In the past decade, researchers have proposed that the pldA gene for outer membrane phospholipase A (OMPLA) is important for bacterial colonization of the human gastric ventricle. Several conserved Helicobacter pylori genes have distinct genotypes in different parts of the world, biogeographic patterns that ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-206
更新日期:2012-09-13 00:00:00
abstract:BACKGROUND:Tsetse flies (Diptera: Glossinidae) are solely responsible for the transmission of African trypanosomes, causative agents of sleeping sickness in humans and nagana in livestock. Due to the lack of efficient vaccines and the emergence of drug resistance, vector control approaches such as the sterile insect te...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1295-4
更新日期:2018-11-23 00:00:00
abstract:BACKGROUND:Typically, raw meat can be contaminated with antibiotic resistant pathogens at unhygienic slaughter and sale points. Consumption of meat contaminated with antibiotic resistant E. coli is associated with grave health care consequences. The aim of this study was to determine the microbial quality of raw meat, ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01935-z
更新日期:2020-08-14 00:00:00
abstract:BACKGROUND:Nitric oxide (NO) is produced as part of the host immune response to bacterial infections, including urinary tract infections. The enzyme flavohemoglobin, coded by the hmp gene, is involved in protecting bacterial cells from the toxic effects of NO and represents a potentially interesting target for developm...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-14-65
更新日期:2014-03-14 00:00:00
abstract:BACKGROUND:The RNA interference (RNAi) pathway acts as an innate antiviral immune response in Aedes aegypti, modulating arbovirus infection of mosquitoes. Sindbis virus (SINV; family: Togaviridae, genus: Alphavirus) is an arbovirus that infects Ae. aegypti in the laboratory. SINV strain TR339 encounters a midgut escape...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-130
更新日期:2010-04-28 00:00:00
abstract:BACKGROUND:During June-July 2012, six imipenem-resistant Escherichia coli isolates were isolated from two patients hospitalized in a ward of one large tertiary-care hospital in Genoa, Italy. Genetic features associated with blaNDM-4 gene were investigated. RESULTS:The isolates exhibited the same PFGE profile and a mul...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-14-148
更新日期:2014-06-07 00:00:00
abstract:BACKGROUND:Staphylococcus aureus is an opportunistic commensal bacterium that mostly colonizes the skin and soft tissues. The pathogenicity of S. aureus is due to both its ability to resist antibiotics, and the production of toxins. Here, we characterize a group of genes responsible for toxin production and antibiotic ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-188
更新日期:2013-08-08 00:00:00
abstract:BACKGROUND:Many plants have been used in traditional medicine for their antibacterial, antifungal, antiprotozoal, antiviral, antidiarrhoeal, analgesic, antimalarial, antioxidant, anti-inflammatory and anticancer activities. In order to find novel antimicrobial and antiviral agents, the aim of the present study was the ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-1068-5
更新日期:2017-07-14 00:00:00
abstract:BACKGROUND:It is known that insects and crustaceans can carry simultaneous, active infections of two or more viruses without showing signs of disease, but it was not clear whether co-infecting viruses occupied the same cells or different cells in common target tissues. Our previous work showed that successive challenge...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-14
更新日期:2010-01-20 00:00:00
abstract:BACKGROUND:Lactobacillus species are used as probiotics and play an important role in fermented food production. However, use of 16S rRNA gene sequences as standard markers for the differentiation of Lactobacillus species offers a very limited scope, as several species of Lactobacillus share similar 16S rRNA gene seque...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01781-z
更新日期:2020-04-15 00:00:00
abstract:BACKGROUND:Mannheimia haemolytica strains isolated from North American cattle have been classified into two genotypes (1 and 2). Although members of both genotypes have been isolated from the upper and lower respiratory tracts of cattle with or without bovine respiratory disease (BRD), genotype 2 strains are much more ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01932-2
更新日期:2020-08-12 00:00:00
abstract:BACKGROUND:Chlamydia trachomatis, an obligate intracellular human pathogen, is the most prevalent bacterial sexually transmitted infection worldwide and a leading cause of preventable blindness. HtrA is a virulence and stress response periplasmic serine protease and molecular chaperone found in many bacteria. Recombina...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-190
更新日期:2008-11-06 00:00:00
abstract:BACKGROUND:Candida krusei has been known to exhibit communal interactions such as pellicle formation and crawling out of nutritional broth. We noticed another possible interaction on agar surfaces, where C. krusei yeast cells formed mycelia along agar surfaces toward each other. We report here the results of experiment...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-0972-z
更新日期:2017-03-11 00:00:00