Allele distribution and genetic diversity of VNTR loci in Salmonella enterica serotype Enteritidis isolates from different sources.

Abstract:

BACKGROUND:Salmonella enterica serotype Enteritidis (S. Enteritidis) is a zoonotic pathogen, which can be found in many sources including animals and the environment. However, little is known about the molecular relatedness among S. Enteritidis isolates from different sources. We have applied multiple-locus variable number tandem repeat analysis (MLVA) to study the genetic diversity of S. Enteritidis isolates from human and non-human sources. RESULTS:We identified 38 unique MLVA types using nine VNTR loci markers for discrimination between 145 S. Enteritidis isolates from different sources including humans (n = 41), chickens (n = 45), and eggs (n = 40). There were 20 distinct MLVA types identified from human isolates, 17 distinct MLVA types from chicken isolates, and 5 from egg isolates. We compared allele distribution and frequency for each VNTR marker and measured allelic polymorphism within each VNTR locus of S. Enteritidis isolates from the sources using Nei's diversity index (D). Differences in allele distribution and frequency were detected in most loci of study isolates. Different genetic diversity for certain loci was identified in isolates from different sources. The average of genetic diversity (D) was lower in egg isolates (0.16) compared to human (0.41) and chicken (0.30). However, for loci SE3, SE7, and SE9, human isolates showed significantly higher diversity than both chicken and egg isolates. Whereas for loci SE5 and SE10, chicken isolates had significantly higher diversity than both human and egg isolates. Minimum-spanning tree (MST) comprised one major cluster, a minor cluster, and four clonal expansions. MLVA application enabled a cluster analysis by the MST of the S. Enteritidis isolates by sources, which allows a great insight into the genetic relatedness and the possible flow of these organisms between different reservoirs and humans. CONCLUSION:Differences in allele distribution and genetic diversity of VNTR loci in S. Enteritidis isolates from different sources were found. Polymorphism in most of the VNTR loci was more frequent among human S. Enteritidis isolates than isolates from chickens or eggs. Therefore, VNTR profiles of S. Enteritidis isolates from a specific source should be further evaluated as potential markers in epidemiologic investigations to trace S. Enteritidis to their probable source.

journal_name

BMC Microbiol

journal_title

BMC microbiology

authors

Cho S,Whittam TS,Boxrud DJ,Bartkus JM,Saeed AM

doi

10.1186/1471-2180-8-146

subject

Has Abstract

pub_date

2008-09-15 00:00:00

pages

146

issn

1471-2180

pii

1471-2180-8-146

journal_volume

8

pub_type

杂志文章
  • The microbiota in the intestinal and respiratory tracts of naked mole-rats revealed by high-throughput sequencing.

    abstract:BACKGROUND:The naked mole-rat (NMR, Heterocephalus glaber) is being bred as a novel laboratory animal due to its unique biological characteristics, including longevity, cancer resistance, hypoxia tolerance, and pain insensitivity. It is expected that differences exist between the microbiota of wild NMRs and that of NMR...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1226-4

    authors: Cong W,Xing J,Feng Y,Wang J,Fu R,Yue B,He Z,Lin L,Yang W,Cheng J,Sun W,Cui S

    更新日期:2018-08-22 00:00:00

  • Bacterial diversity analysis of larvae and adult midgut microflora using culture-dependent and culture-independent methods in lab-reared and field-collected Anopheles stephensi-an Asian malarial vector.

    abstract:BACKGROUND:Mosquitoes are intermediate hosts for numerous disease causing organisms. Vector control is one of the most investigated strategy for the suppression of mosquito-borne diseases. Anopheles stephensi is one of the vectors of malaria parasite Plasmodium vivax. The parasite undergoes major developmental and matu...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-96

    authors: Rani A,Sharma A,Rajagopal R,Adak T,Bhatnagar RK

    更新日期:2009-05-19 00:00:00

  • Nuclear and Wolbachia-based multimarker approach for the rapid and accurate identification of tsetse species.

    abstract:BACKGROUND:Tsetse flies (Diptera: Glossinidae) are solely responsible for the transmission of African trypanosomes, causative agents of sleeping sickness in humans and nagana in livestock. Due to the lack of efficient vaccines and the emergence of drug resistance, vector control approaches such as the sterile insect te...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1295-4

    authors: Augustinos AA,Meki IK,Demirbas-Uzel G,Ouédraogo GMS,Saridaki A,Tsiamis G,Parker AG,Abd-Alla AMM,Bourtzis K

    更新日期:2018-11-23 00:00:00

  • Pyviko: an automated Python tool to design gene knockouts in complex viruses with overlapping genes.

    abstract:BACKGROUND:Gene knockouts are a common tool used to study gene function in various organisms. However, designing gene knockouts is complicated in viruses, which frequently contain sequences that code for multiple overlapping genes. Designing mutants that can be traced by the creation of new or elimination of existing r...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-016-0920-3

    authors: Taylor LJ,Strebel K

    更新日期:2017-01-07 00:00:00

  • Heat shock cognate protein 70 contributes to Brucella invasion into trophoblast giant cells that cause infectious abortion.

    abstract:BACKGROUND:The cell tropism of Brucella abortus, a causative agent of brucellosis and facultative intracellular pathogen, in the placenta is thought to be a key event of infectious abortion, although the molecular mechanism for this is largely unknown. There is a higher degree of bacterial colonization in the placenta ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-212

    authors: Watanabe K,Tachibana M,Tanaka S,Furuoka H,Horiuchi M,Suzuki H,Watarai M

    更新日期:2008-12-05 00:00:00

  • Predictive analysis of transmissible quinolone resistance indicates Stenotrophomonas maltophilia as a potential source of a novel family of Qnr determinants.

    abstract:BACKGROUND:Predicting antibiotic resistance before it emerges at clinical settings constitutes a novel approach for preventing and fighting resistance of bacterial pathogens. To analyse the possibility that novel plasmid-encoded quinolone resistance determinants (Qnr) can emerge and disseminate among bacterial pathogen...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-8-148

    authors: Sánchez MB,Hernández A,Rodríguez-Martínez JM,Martínez-Martínez L,Martínez JL

    更新日期:2008-09-16 00:00:00

  • Genetic diversity of Listeria monocytogenes strains in ruminant abortion and rhombencephalitis cases in comparison with the natural environment.

    abstract:BACKGROUND:Listeria monocytogenes is the causative agent of listeriosis, a serious disease affecting both animals and humans. Here, multilocus sequence typing (MLST) was used to characterize the genetic diversity of Listeria monocytogenes strains isolated from the natural environment and animal clinical cases in Europe...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1676-3

    authors: Papić B,Pate M,Félix B,Kušar D

    更新日期:2019-12-18 00:00:00

  • Evolutionary relationships of ATP-Binding Cassette (ABC) uptake porters.

    abstract:BACKGROUND:The ATP-Binding Cassette (ABC) functional superfamily includes integral transmembrane exporters that have evolved three times independently, forming three families termed ABC1, ABC2 and ABC3, upon which monophyletic ATPases have been superimposed for energy-coupling purposes [e.g., J Membr Biol 231(1):1-10, ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-98

    authors: Zheng WH,Västermark Å,Shlykov MA,Reddy V,Sun EI,Saier MH Jr

    更新日期:2013-05-06 00:00:00

  • Genotyping and phylogenetic placement of Bacillus anthracis isolates from Finland, a country with rare anthrax cases.

    abstract:BACKGROUND:Anthrax, the zoonotic disease caused by the gram-positive bacterium Bacillus anthracis, is nowadays rare in northern parts of Europe including Finland and Scandinavia. Only two minor outbreaks of anthrax in 1988 and in 2004 and one sporadic infection in 2008 have been detected in animals in Finland since the...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1250-4

    authors: Lienemann T,Beyer W,Pelkola K,Rossow H,Rehn A,Antwerpen M,Grass G

    更新日期:2018-09-03 00:00:00

  • Staphylococcus aureus Biofilm and Planktonic cultures differentially impact gene expression, mapk phosphorylation, and cytokine production in human keratinocytes.

    abstract:BACKGROUND:Many chronic diseases, such as non-healing wounds are characterized by prolonged inflammation and respond poorly to conventional treatment. Bacterial biofilms are a major impediment to wound healing. Persistent infection of the skin allows the formation of complex bacterial communities termed biofilm. Bacter...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-11-143

    authors: Secor PR,James GA,Fleckman P,Olerud JE,McInnerney K,Stewart PS

    更新日期:2011-06-21 00:00:00

  • Functional significance of nuclear export and mRNA binding of meiotic regulator Spo5 in fission yeast.

    abstract:BACKGROUND:Meiotic cells undergo two rounds of nuclear division and generate gametes. Previous studies have indicated that a number of transcription factors modulate the transcriptome in successive waves during meiosis and spore formation in fission yeast. However, the mechanisms underlying the post-transcriptional reg...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-14-188

    authors: Togashi N,Yamashita A,Sato M,Yamamoto M

    更新日期:2014-07-15 00:00:00

  • Broad-range potential of Asphodelus microcarpus leaves extract for drug development.

    abstract:BACKGROUND:Many plants have been used in traditional medicine for their antibacterial, antifungal, antiprotozoal, antiviral, antidiarrhoeal, analgesic, antimalarial, antioxidant, anti-inflammatory and anticancer activities. In order to find novel antimicrobial and antiviral agents, the aim of the present study was the ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-017-1068-5

    authors: Di Petrillo A,Fais A,Pintus F,Santos-Buelga C,González-Paramás AM,Piras V,Orrù G,Mameli A,Tramontano E,Frau A

    更新日期:2017-07-14 00:00:00

  • Kinin release from human kininogen by 10 aspartic proteases produced by pathogenic yeast Candida albicans.

    abstract:BACKGROUND:Candida albicans yeast produces 10 distinct secreted aspartic proteases (Saps), which are some of the most important virulence factors of this pathogenic fungus. One of the suggested roles of Saps is their deregulating effect on various proteolytic cascades that constitute the major homeostatic systems in hu...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-015-0394-8

    authors: Kozik A,Gogol M,Bochenska O,Karkowska-Kuleta J,Wolak N,Kamysz W,Aoki W,Ueda M,Faussner A,Rapala-Kozik M

    更新日期:2015-03-04 00:00:00

  • Characterization of the bacterial communities of psyllids associated with Rutaceae in Bhutan by high throughput sequencing.

    abstract:BACKGROUND:Several plant-pathogenic bacteria are transmitted by insect vector species that often also act as hosts. In this interface, these bacteria encounter plant endophytic, insect endosymbiotic and other microbes. Here, we used high throughput sequencing to examine the bacterial communities of five different psyll...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-01895-4

    authors: Morrow JL,Om N,Beattie GAC,Chambers GA,Donovan NJ,Liefting LW,Riegler M,Holford P

    更新日期:2020-07-20 00:00:00

  • Biological and transcriptomic studies reveal hfq is required for swimming, biofilm formation and stress response in Xanthomonas axonpodis pv. citri.

    abstract:BACKGROUND:Hfq is a widely conserved bacterial RNA-binding protein which generally mediates the global regulatory activities involv ed in physiological process and virulence. The goal of this study was to characterize the biological function of hfq gene in Xanthomonas axonpodis pv. citri (Xac), the causal agent of citr...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1476-9

    authors: Liu X,Yan Y,Wu H,Zhou C,Wang X

    更新日期:2019-05-22 00:00:00

  • Roles of iron acquisition systems in virulence of extraintestinal pathogenic Escherichia coli: salmochelin and aerobactin contribute more to virulence than heme in a chicken infection model.

    abstract:BACKGROUND:Avian pathogenic Escherichia coli (APEC) and uropathogenic E. coli (UPEC) are the two main subsets of extraintestinal pathogenic E. coli (ExPEC). Both types have multiple iron acquisition systems, including heme and siderophores. Although iron transport systems involved in the pathogenesis of APEC or UPEC ha...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-12-143

    authors: Gao Q,Wang X,Xu H,Xu Y,Ling J,Zhang D,Gao S,Liu X

    更新日期:2012-07-20 00:00:00

  • Long-term impact of fecal transplantation in healthy volunteers.

    abstract:BACKGROUND:Fecal microbiota transplantation (FMT) has been recently approved by FDA for the treatment of refractory recurrent clostridial colitis (rCDI). Success of FTM in treatment of rCDI led to a number of studies investigating the effectiveness of its application in the other gastrointestinal diseases. However, in ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1689-y

    authors: Goloshchapov OV,Olekhnovich EI,Sidorenko SV,Moiseev IS,Kucher MA,Fedorov DE,Pavlenko AV,Manolov AI,Gostev VV,Veselovsky VA,Klimina KM,Kostryukova ES,Bakin EA,Shvetcov AN,Gumbatova ED,Klementeva RV,Shcherbakov AA,Gorchak

    更新日期:2019-12-30 00:00:00

  • Microbial contamination and chemical toxicity of the Rio Grande.

    abstract:BACKGROUND:The Rio Grande River is the natural boundary between U.S. and Mexico from El Paso, TX to Brownsville, TX. and is one of the major water resources of the area. Agriculture, farming, maquiladora industry, domestic activities, as well as differences in disposal regulations and enforcement increase the contamina...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-4-17

    authors: Mendoza J,Botsford J,Hernandez J,Montoya A,Saenz R,Valles A,Vazquez A,Alvarez M

    更新日期:2004-04-22 00:00:00

  • atpE gene as a new useful specific molecular target to quantify Mycobacterium in environmental samples.

    abstract:BACKGROUND:The environment is the likely source of many pathogenic mycobacterial species but detection of mycobacteria by bacteriological tools is generally difficult and time-consuming. Consequently, several molecular targets based on the sequences of housekeeping genes, non-functional RNA and structural ribosomal RNA...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-13-277

    authors: Radomski N,Roguet A,Lucas FS,Veyrier FJ,Cambau E,Accrombessi H,Moilleron R,Behr MA,Moulin L

    更新日期:2013-12-03 00:00:00

  • Molecular profiling of fungal communities in moisture damaged buildings before and after remediation--a comparison of culture-dependent and culture-independent methods.

    abstract:BACKGROUND:Indoor microbial contamination due to excess moisture is an important contributor to human illness in both residential and occupational settings. However, the census of microorganisms in the indoor environment is limited by the use of selective, culture-based detection techniques. By using clone library sequ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-11-235

    authors: Pitkäranta M,Meklin T,Hyvärinen A,Nevalainen A,Paulin L,Auvinen P,Lignell U,Rintala H

    更新日期:2011-10-21 00:00:00

  • The Firmicutes/Bacteroidetes ratio of the human microbiota changes with age.

    abstract:BACKGROUND:In humans, the intestinal microbiota plays an important role in the maintenance of host health by providing energy, nutrients, and immunological protection. Applying current molecular methods is necessary to surmount the limitations of classical culturing techniques in order to obtain an accurate description...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-9-123

    authors: Mariat D,Firmesse O,Levenez F,Guimarăes V,Sokol H,Doré J,Corthier G,Furet JP

    更新日期:2009-06-09 00:00:00

  • Genome-wide expression profiling of the response to short-term exposure to fluconazole in Cryptococcus neoformans serotype A.

    abstract:BACKGROUND:Fluconazole (FLC), a triazole antifungal drug, is widely used for the maintenance therapy of cryptococcal meningoencephalitis, the most common opportunistic infection in AIDS patients. In this study, we examined changes in the gene expression profile of the C. neoformans reference strain H99 (serotype A) fol...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-11-97

    authors: Florio AR,Ferrari S,De Carolis E,Torelli R,Fadda G,Sanguinetti M,Sanglard D,Posteraro B

    更新日期:2011-05-11 00:00:00

  • Virulence determinants of Pseudomonas syringae strains isolated from grasses in the context of a small type III effector repertoire.

    abstract:BACKGROUND:Pseudomonas syringae is pathogenic to a large number of plant species. For host colonization and disease progression, strains of this bacterium utilize an array of type III-secreted effectors and other virulence factors, including small secreted molecules such as syringolin A, a peptide derivative that inhib...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-014-0304-5

    authors: Dudnik A,Dudler R

    更新日期:2014-12-04 00:00:00

  • Association of Bordetella dermonecrotic toxin with the extracellular matrix.

    abstract:BACKGROUND:Bordetella dermonecrotic toxin (DNT) causes the turbinate atrophy in swine atrophic rhinitis, caused by a Bordetella bronchiseptica infection of pigs, by inhibiting osteoblastic differentiation. The toxin is not actively secreted from the bacteria, and is presumed to be present in only small amounts in infec...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-10-247

    authors: Fukui-Miyazaki A,Kamitani S,Miyake M,Horiguchi Y

    更新日期:2010-09-25 00:00:00

  • Biological characterization of Pasteurella multocida present in the Saiga population.

    abstract:BACKGROUND:This study provides biochemical and molecular genetic characteristics of P. multocida isolated from dead saigas in 1988, 2010-2015 on the territory of the Republic of Kazakhstan. RESULTS:Bacteriological samples taken from carcasses of saiga antelope during mortality events recorded in West Kazakhstan in bot...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-019-1407-9

    authors: Orynbayev M,Sultankulova K,Sansyzbay A,Rystayeva R,Shorayeva K,Namet A,Fereidouni S,Ilgekbayeva G,Barakbayev K,Kopeyev S,Kock R

    更新日期:2019-02-11 00:00:00

  • In silico evolutionary analysis of Helicobacter pylori outer membrane phospholipase A (OMPLA).

    abstract:BACKGROUND:In the past decade, researchers have proposed that the pldA gene for outer membrane phospholipase A (OMPLA) is important for bacterial colonization of the human gastric ventricle. Several conserved Helicobacter pylori genes have distinct genotypes in different parts of the world, biogeographic patterns that ...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-12-206

    authors: Vollan HS,Tannaes T,Yamaoka Y,Bukholm G

    更新日期:2012-09-13 00:00:00

  • Analysis of virulence phenotypes and antibiotic resistance in clinical strains of Acinetobacter baumannii isolated in Nashville, Tennessee.

    abstract:BACKGROUND:Acinetobacter baumannii is a gram-negative bacterium which causes opportunistic infections in immunocompromised hosts. Genome plasticity has given rise to a wide range of strain variation with respect to antimicrobial resistance profiles and expression of virulence factors which lead to altered phenotypes as...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-020-02082-1

    authors: Boone RL,Whitehead B,Avery TM,Lu J,Francis JD,Guevara MA,Moore RE,Chambers SA,Doster RS,Manning SD,Townsend SD,Dent L,Marshall D,Gaddy JA,Damo SM

    更新日期:2021-01-09 00:00:00

  • Analysis of stress- and host cell-induced expression of the Mycobacterium tuberculosis inorganic pyrophosphatase.

    abstract:BACKGROUND:The gene encoding the inorganic pyrophosphatase (PPase) of the intracellular pathogen Legionella pneumophila is induced during intracellular infection, but is constitutively expressed in Escherichia coli. The causative agent of tuberculosis, Mycobacterium tuberculosis, contains a well conserved copy of PPase...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/1471-2180-1-3

    authors: Triccas JA,Gicquel B

    更新日期:2001-01-01 00:00:00

  • Diversity of Mycobacterium tuberculosis and drug resistance in different provinces of Papua New Guinea.

    abstract:BACKGROUND:Papua New Guinea (PNG) is a high tuberculosis (TB) burden country of the WHO Western Pacific Region, but so far research on drug resistance (DR) and genotypes of Mycobacterium tuberculosis (M. tuberculosis) was only conducted in few provinces in the country. The aim of the present study was to obtain baselin...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-014-0307-2

    authors: Ley SD,Harino P,Vanuga K,Kamus R,Carter R,Coulter C,Pandey S,Feldmann J,Ballif M,Siba PM,Phuanukoonnon S,Gagneux S,Beck HP

    更新日期:2014-12-05 00:00:00

  • A metabolomics and proteomics study of the Lactobacillus plantarum in the grass carp fermentation.

    abstract:BACKGROUND:Lactobacillus plantarum, a versatile lactic acid-fermenting bacterium, isolated from the traditional pickles in Ningbo of China, was chosen for grass carp fermentation, which could also improve the flavor of grass carp. We here explored the central metabolic pathways of L. plantarum by using metabolomic appr...

    journal_title:BMC microbiology

    pub_type: 杂志文章

    doi:10.1186/s12866-018-1354-x

    authors: Ming T,Han J,Li Y,Lu C,Qiu D,Li Y,Zhou J,Su X

    更新日期:2018-12-18 00:00:00