Abstract:
BACKGROUND:Ophiocordyceps sinensis (DongChong XiaCao (DCXC) in Chinese), a fungal parasite of caterpillars, is a traditional Chinese medicine. Bioactive components isolated from natural DCXC possess a wide range of pharmacological actions. Many efforts have been directed towards isolating the fungi based on culture-dependent methods for investigation of fungal diversity in order to determine the anamorph of natural DCXC and find new medicinal fungi resources, and the results have been varied. RESULTS:In the present study, a total of 44,588 bacterial and 51,584 fungal sequences corresponding to 11,694 and 9297 putative operational taxonomic units (OTU) were respectively identified by a Roche/454-based, high throughput sequence analysis of 16S rRNA genes and ITS regions. The main bacterial groups were Proteobacteria, Acidobacteria, Bacteroidetes, Actinobacteria and Firmicutes, while the Ascomycota, Basidiomycota and Zygomycota were the main fungal phyla. Proteobacteria presented 68.4, 49.5, 38.9 and 35.6 % of all bacteria in the sclerotia, stromata, external mycelial cortices and soil, respectively. As the main fungi phyla, Ascomycota presented 21.0, 45.6 26.4 and 59.3 % in the sclerotia, stromata, external mycelial cortices and soil, respectively. Bacterial and fungal communities were more diverse in the environmental sample than in the natural DCXC sample. Microbial communities were obviously distinct in each sample. Several novel unclassifiable bacterial (10.41 %) and fungal (37.92 %) species were also detected. CONCLUSIONS:This study revealed an abundant endogenetic fungal and bacterial resources and a variety of genetic information in natural DCXC by high-throughput 454 sequencing technology. Microorganism that had been discovered in natural DCXC will provide sources for screening the new bioactive metabolites and its biotechnological application.
journal_name
BMC Microbioljournal_title
BMC microbiologyauthors
Xia F,Liu Y,Guo MY,Shen GR,Lin J,Zhou XWdoi
10.1186/s12866-016-0813-5subject
Has Abstractpub_date
2016-08-26 00:00:00pages
196issue
1issn
1471-2180pii
10.1186/s12866-016-0813-5journal_volume
16pub_type
杂志文章相关文献
BMC MICROBIOLOGY文献大全abstract:BACKGROUND:Bacteria employ multiple mechanisms to control gene expression and react to their constantly changing environment. Bacterial growth in rich laboratory medium is a dynamic process in which bacteria utilize nutrients from simple to complex and change physical properties of the medium, as pH, during the process...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-32
更新日期:2009-02-10 00:00:00
abstract:BACKGROUND:The antibiotics generally used in farm animals are rapidly losing their effectiveness all over the world as bacteria develop antibiotic resistance. Like some other pathogenic bacteria multidrug-resistant strains of Salmonella enterica serovar Typhimurium (S. Typhimurium) are also frequently found in animals ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01810-x
更新日期:2020-05-14 00:00:00
abstract:BACKGROUND:Non-ribosomal peptide synthetase (NRPS) enzymes govern the assembly of amino acids and related monomers into peptide-like natural products. A key goal of the field is to develop methods to effective recombine NRPS domains or modules, and thereby generate modified or entirely novel products. We previously sho...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0496-3
更新日期:2015-08-13 00:00:00
abstract:BACKGROUND:The aminoglycoside-resistance genes encoding aminoglycoside modifying enzymes and 16S rRNA methyltransferases are main factors contributing to increasing resistance to aminoglycosides. Characterization and distribution of antimicrobial resistance gene profiles provide important information on the potential d...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-58
更新日期:2013-03-14 00:00:00
abstract:BACKGROUND:Globally, staphylococci have developed resistance to many antibiotics. New approaches to chemotherapy are needed and one such approach could be to use plant derived actives with conventional antibiotics in a synergestic way. The purpose of this study was to isolate α-mangostin from the mangosteen (Garcinia m...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0814-4
更新日期:2016-08-26 00:00:00
abstract:BACKGROUND:Zinc is an essential element for all living cells. Recent studies have shown that the ZnuABC zinc uptake system significantly contributes to the ability of several pathogens to multiply in the infected host and cause disease, suggesting that zinc is scarcely available within different tissues of the host. To...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-11-36
更新日期:2011-02-21 00:00:00
abstract:BACKGROUND:Most studies on the human microbiota have analyzed stool samples, although a large proportion of the absorption of nutrients takes place in upper gut tract. We collected samples from different locations along the entire gastrointestinal tract from six patients who had simultaneously undergone upper endoscopy...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1304-7
更新日期:2018-10-24 00:00:00
abstract:BACKGROUND:The naked mole-rat (NMR, Heterocephalus glaber) is being bred as a novel laboratory animal due to its unique biological characteristics, including longevity, cancer resistance, hypoxia tolerance, and pain insensitivity. It is expected that differences exist between the microbiota of wild NMRs and that of NMR...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1226-4
更新日期:2018-08-22 00:00:00
abstract:BACKGROUND:Brucellosis is an important zoonosis caused by the genus Brucella. In addition Brucella represents potential biological warfare agents due to the high contagious rates for humans and animals. Therefore, the strain typing epidemiological tool may be crucial for tracing back source of infection in outbreaks an...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-66
更新日期:2009-04-07 00:00:00
abstract:BACKGROUND:The KdpD/KdpE two-component system of Escherichia coli regulates expression of the kdpFABC operon encoding the high affinity K+ transport system KdpFABC. The input domain of KdpD comprises a domain that belongs to the family of universal stress proteins (Usp). It has been previously demonstrated that UspC bi...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-133
更新日期:2009-07-09 00:00:00
abstract:BACKGROUND:Lactobacillus species are used as probiotics and play an important role in fermented food production. However, use of 16S rRNA gene sequences as standard markers for the differentiation of Lactobacillus species offers a very limited scope, as several species of Lactobacillus share similar 16S rRNA gene seque...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01781-z
更新日期:2020-04-15 00:00:00
abstract:BACKGROUND:Predicting antibiotic resistance before it emerges at clinical settings constitutes a novel approach for preventing and fighting resistance of bacterial pathogens. To analyse the possibility that novel plasmid-encoded quinolone resistance determinants (Qnr) can emerge and disseminate among bacterial pathogen...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-148
更新日期:2008-09-16 00:00:00
abstract:BACKGROUND:Classical bacteriological characteristics of Salmonella enterica indicate that the members of this species are unable to utilize lactose as a carbon source. However, lactose-fermenting (Lac+) strains of several Salmonella serovars have been isolated from different foodborne outbreaks as well as different geo...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0511-8
更新日期:2015-08-25 00:00:00
abstract:BACKGROUND:Over the last years oleaginous yeasts have been studied for several energetic, oleochemical, medical and pharmaceutical purposes. However, only a small number of yeasts are known and have been deeply exploited. The search for new isolates with high oleaginous capacity becomes imperative, as well as the use o...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01742-6
更新日期:2020-03-14 00:00:00
abstract:BACKGROUND:Saccharomyces cerevisiae multicellular communities are sustained by a scaffolding extracellular matrix, which provides spatial organization, and nutrient and water availability, and ensures group survival. According to this tissue-like biology, the yeast extracellular matrix (yECM) is analogous to the higher...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0550-1
更新日期:2015-11-25 00:00:00
abstract:BACKGROUND:Cow manure is not only an agricultural waste, but also an organic fertilizer resource. The application of organic fertilizer is a feasible practice to mitigate the soil degradation caused by overuse of chemical fertilizers, which can affect the bacterial diversity and community composition in soils. However,...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01871-y
更新日期:2020-07-01 00:00:00
abstract:BACKGROUND:FliH is a protein involved in the export of components of the bacterial flagellum and we herein describe the presence of glycine-rich repeats in FliH of the form AxxxG(xxxG)mxxxA, where the value of m varies considerably in FliH proteins from different bacteria. While GxxxG and AxxxA patterns have previously...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-72
更新日期:2009-04-16 00:00:00
abstract:BACKGROUND:Streptomyces lividans is an appealing host for the production of proteins of biotechnological interest due to its relaxed exogenous DNA restriction system and its ability to secrete proteins directly to the medium through the major Sec or the minor Tat routes. Often, protein secretion displays non-uniform ti...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1591-7
更新日期:2019-10-26 00:00:00
abstract:BACKGROUND:Escherichia coli is a well-studied anaerobic bacteria which is able to regulate metabolic pathways depending on the type of sugar presented in the medium. We have studied the glucose-lactose shift in E. coli at the protein level using a recently developed mass spectrometry platform. METHOD:Cells were grown ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-11-126
更新日期:2011-06-01 00:00:00
abstract:BACKGROUND:Bacterial and cellular genotyping is becoming increasingly important in the diagnosis of infectious diseases. However, difficulties in obtaining sufficient amount of bacterial and cellular DNA extracted from the same human biopsy specimens is often a limiting factor. In this study, total DNA (host and bacter...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-175
更新日期:2008-10-08 00:00:00
abstract:BACKGROUND:The emergence and spread of multidrug resistant methicillin-resistant Staphylococcus aureus (MDR-MRSA) has serious health consequences in the presence of sub-MIC antibiotics. Therefore, this study was designed to evaluate β-lactamase activity, efflux activity, biofilm formation, and gene expression pattern i...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0791-7
更新日期:2016-07-29 00:00:00
abstract:BACKGROUND:The aim of this study was to identify mutations of rpoB, katG, inhA and ahp-genes associated Mycobacterium tuberculosis resistance to rifampicin (RIF) and isoniazid (INH) in Kyrgyz Republic. We studied 633 smear samples from the primary pulmonary tuberculosis (TB) patients. We verified Mycobacterium tubercul...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1168-x
更新日期:2018-03-22 00:00:00
abstract:BACKGROUND:The life cycle of a bacteriophage has tightly programmed steps to help virus infect its host through the interactions between the bacteriophage and its host proteins. However, bacteriophage-host protein interactions in high temperature environment remain poorly understood. To address this issue, the protein ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-48
更新日期:2013-02-26 00:00:00
abstract:BACKGROUND:The evolution of mycoplasmas from a common ancestor with Firmicutes has been characterized not only by genome down-sizing but also by horizontal gene transfer between mycoplasma species sharing a common host. The mechanisms of these gene transfers remain unclear because our knowledge of the mycoplasma mobile...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-257
更新日期:2012-11-12 00:00:00
abstract:BACKGROUND:Mycobacterium avium is an environmental mycobacterium that can be divided into the subspecies avium, hominissuis, paratuberculosis and silvaticum. Some M. avium subspecies are opportunistic pathogens for animals and humans. They are ubiquitous in nature and can be isolated from natural sources of water, soil...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-7-14
更新日期:2007-03-05 00:00:00
abstract:ERRATUM:Upon publication of the original article (1) it was highlighted by the authors that a grant awarded to support the research work of the study was missed in the acknowledgements. It should also be acknowledged that the grant titled "Genotyping and Molecular Epidemiological Characteristic of Bacillus anthracis in...
journal_title:BMC microbiology
pub_type: 杂志文章,已发布勘误
doi:10.1186/s12866-018-1172-1
更新日期:2018-04-12 00:00:00
abstract:BACKGROUND:The Beijing lineage of Mycobacterium tuberculosis is causing concern due to its global distribution and its involvement in severe outbreaks. Studies focused on this lineage are mainly restricted to geographical settings where its prevalence is high, whereas those in other areas are scarce. In this study, we ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-151
更新日期:2010-05-25 00:00:00
abstract:BACKGROUND:The environment is the likely source of many pathogenic mycobacterial species but detection of mycobacteria by bacteriological tools is generally difficult and time-consuming. Consequently, several molecular targets based on the sequences of housekeeping genes, non-functional RNA and structural ribosomal RNA...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-277
更新日期:2013-12-03 00:00:00
abstract:BACKGROUND:Several plant-pathogenic bacteria are transmitted by insect vector species that often also act as hosts. In this interface, these bacteria encounter plant endophytic, insect endosymbiotic and other microbes. Here, we used high throughput sequencing to examine the bacterial communities of five different psyll...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01895-4
更新日期:2020-07-20 00:00:00
abstract:BACKGROUND:Many plants have been used in traditional medicine for their antibacterial, antifungal, antiprotozoal, antiviral, antidiarrhoeal, analgesic, antimalarial, antioxidant, anti-inflammatory and anticancer activities. In order to find novel antimicrobial and antiviral agents, the aim of the present study was the ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-1068-5
更新日期:2017-07-14 00:00:00