Abstract:
BACKGROUND:The aim of this study was to identify mutations of rpoB, katG, inhA and ahp-genes associated Mycobacterium tuberculosis resistance to rifampicin (RIF) and isoniazid (INH) in Kyrgyz Republic. We studied 633 smear samples from the primary pulmonary tuberculosis (TB) patients. We verified Mycobacterium tuberculosis susceptibility to RIF and INH using culture method of absolute concentrations, and commercially available test named "TB-BIOCHIP" (Biochip-IMB, Moscow, Russian Federation). RESULTS:For RIF-resistance, TB-BIOCHIP's sensitivity and specificity were 88% and 97%, 84% and 95% for INH-resistance, and 90% and 97% for multi-drug resistance (MDR). In RIF-resistant strains, TB-BIOCHIP showed mutations in codons 531 (64.8%), 526 (17.3%), 516 (8.1%), 511 (5.4%), 533 (3.2%), 522 (0.6%) and 513 (0.6%) of rpoB gene. The most prevalent was Ser531 > Leu mutation (63.7%). 91.2% of mutations entailing resistance to INH were in katG gene, 7% in inhA gene, and 1.8% in ahpC gene. Ser315→Thr (88.6%) was the most prevalent mutation leading to resistance to INH. CONCLUSIONS:In Kyrgyz Republic, the most prevalent mutation in RIF-resistant strains was Ser531 → Leu in rpoB gene, as opposed to Ser315 → Thr in katG gene in INH-resistant Mycobacterium tuberculosis. In Kyrgyz Republic, the major reservoir of MDR Mycobacterium tuberculosis were strains with combined mutations Ser531 → Leu in rpoB gene and Ser315 → Thr in katG gene. TB-BIOCHIP has shown moderate sensitivity with the advantage of obtaining results in only two days.
journal_name
BMC Microbioljournal_title
BMC microbiologyauthors
Isakova J,Sovkhozova N,Vinnikov D,Goncharova Z,Talaibekova E,Aldasheva N,Aldashev Adoi
10.1186/s12866-018-1168-xsubject
Has Abstractpub_date
2018-03-22 00:00:00pages
22issue
1issn
1471-2180pii
10.1186/s12866-018-1168-xjournal_volume
18pub_type
杂志文章相关文献
BMC MICROBIOLOGY文献大全abstract:BACKGROUND:Clostridium termitidis CT1112 is an anaerobic, Gram-positive, mesophilic, spore-forming, cellulolytic bacterium, originally isolated from the gut of a wood feeding termite Nasusitermes lujae. It has the ability to hydrolyze both cellulose and hemicellulose, and ferment the degradation products to acetate, fo...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0711-x
更新日期:2016-05-23 00:00:00
abstract:BACKGROUND:Bacteria release a wide variety of small molecules including cell-to-cell signaling compounds. Gram-negative bacteria use a variety of self-produced autoinducers such as acylated homoserine lactones (acyl-HSLs) as signal compounds for quorum sensing (QS) within and between bacterial species. QS plays a signi...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-70
更新日期:2012-05-10 00:00:00
abstract:BACKGROUND:Vibrio cholerae is the causative agent of cholera. Extensive studies reveal that complicated regulatory cascades regulate expression of virulence genes, the products of which are required for V. cholerae to colonize and cause disease. In this study, we investigated the expression of the key virulence regulat...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-3
更新日期:2010-01-06 00:00:00
abstract:BACKGROUND:Klebsiella pneumoniae displaying the hypermucoviscosity (HV) phenotype are considered more virulent than HV-negative strains. Nevertheless, the emergence of tissue-abscesses-associated HV-negative isolates motivated us to re-evaluate the role of HV-phenotype. RESULTS:Instead of genetically manipulating the ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-11-50
更新日期:2011-03-08 00:00:00
abstract:BACKGROUND:Most studies on the human microbiota have analyzed stool samples, although a large proportion of the absorption of nutrients takes place in upper gut tract. We collected samples from different locations along the entire gastrointestinal tract from six patients who had simultaneously undergone upper endoscopy...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1304-7
更新日期:2018-10-24 00:00:00
abstract:BACKGROUND:Infections caused by Shewanella spp. have been increasingly reported worldwide. The advances in genomic sciences have enabled better understanding about the taxonomy and epidemiology of this agent. However, the scarcity of DNA sequencing data is still an obstacle for understanding the genus and its associati...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-02040-x
更新日期:2020-11-24 00:00:00
abstract:BACKGROUND:Suppression subtractive hybridization (SSH) strategy was used with extraintestinal pathogenic Escherichia coli (EXPEC) that cause avian colibacillosis (avian pathogenic E. coli or APEC) and human urinary tract infections (uropathogenic E. coli or UPEC) to determine if they possessed genes that were host and/...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-7-81
更新日期:2007-08-30 00:00:00
abstract:BACKGROUND:The life cycle of a bacteriophage has tightly programmed steps to help virus infect its host through the interactions between the bacteriophage and its host proteins. However, bacteriophage-host protein interactions in high temperature environment remain poorly understood. To address this issue, the protein ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-48
更新日期:2013-02-26 00:00:00
abstract:BACKGROUND:A common assumption of microorganisms is that laboratory stocks will remain genetically and phenotypically constant over time, and across laboratories. It is becoming increasingly clear, however, that mutations can ruin strain integrity and drive the divergence or "domestication" of stocks. Since its discove...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-14-2
更新日期:2014-01-02 00:00:00
abstract:BACKGROUND:Sinorhizobium meliloti is an agriculturally important model symbiont. There is an ongoing need to update and improve its genome annotation. In this study, we used a high-throughput pyrosequencing approach to sequence the transcriptome of S. meliloti, and search for new bacterial genes missed in the previous ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-72
更新日期:2008-05-02 00:00:00
abstract:BACKGROUND:Enteropathogenic Escherichia coli (EPEC) are classified into typical and atypical strains based on the presence of the E. coli adherence factor (EAF) plasmid. The EAF plasmid contains the bfp (bundle-forming pilus) operon and the perABC (plasmid encoded regulator) gene cluster. A 1-kb cryptic region of EAF p...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0539-9
更新日期:2015-10-05 00:00:00
abstract:BACKGROUND:The ATP-Binding Cassette (ABC) functional superfamily includes integral transmembrane exporters that have evolved three times independently, forming three families termed ABC1, ABC2 and ABC3, upon which monophyletic ATPases have been superimposed for energy-coupling purposes [e.g., J Membr Biol 231(1):1-10, ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-98
更新日期:2013-05-06 00:00:00
abstract:BACKGROUND:Bordetella dermonecrotic toxin (DNT) causes the turbinate atrophy in swine atrophic rhinitis, caused by a Bordetella bronchiseptica infection of pigs, by inhibiting osteoblastic differentiation. The toxin is not actively secreted from the bacteria, and is presumed to be present in only small amounts in infec...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-247
更新日期:2010-09-25 00:00:00
abstract:BACKGROUND:Human granulocytic anaplasmosis (HGA) is a tick-borne disease caused by the etiologic agent Anaplasma phagocytophilum. HGA was designated a nationally notifiable disease in the United States in 1998. Currently there are no vaccines available against HGA. Conserved membrane proteins that are subdominant in An...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1346-x
更新日期:2018-12-18 00:00:00
abstract:BACKGROUND:Salmonella enterica and Campylobacter jejuni are amongst the more prevalent bacterial pathogens that cause foodborne diseases. These microorganisms are common contaminants of poultry and poultry products. This study was aimed to evaluate the antibacterial activity of metallic copper surfaces on these importa...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-4-19
更新日期:2004-04-30 00:00:00
abstract:BACKGROUND:A disrupted cell cycle progression of hepatocytes was reported in chronic hepatitis C virus (HCV) infection, which can contribute significantly in the associated pathogenesis. The present study aimed to further elaborate these disruptions by evaluating the expression of key cell cycle and apoptotic proteins ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-133
更新日期:2008-08-05 00:00:00
abstract::Following publication of the original article [1], the authors reported an error in Fig. 2c. ...
journal_title:BMC microbiology
pub_type: 已发布勘误
doi:10.1186/s12866-020-01791-x
更新日期:2020-04-30 00:00:00
abstract:BACKGROUND:Recent studies have identified in Mycobacterium avium subsp. paratuberculosis (MAP), already known as a pathogen in ruminants, a potential zoonotic agent of some autoimmune diseases in humans. Therefore, considering the possible risk for public health, it is necessary a thorough understanding of MAP's gene e...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-87
更新日期:2012-05-30 00:00:00
abstract:BACKGROUND:Candida parapsilosis typically is a commensal of human skin. However, when host immune defense is compromised or the normal microflora balance is disrupted, C. parapsilosis transforms itself into an opportunistic pathogen. Candida-derived lipase has been identified as potential virulence factor. Even though ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-11-122
更新日期:2011-05-29 00:00:00
abstract:BACKGROUND:Bacillus sp. H2O-1, isolated from the connate water of a Brazilian reservoir, produces an antimicrobial substance (denoted as AMS H2O-1) that is active against sulfate reducing bacteria, which are the major bacterial group responsible for biogenic souring and biocorrosion in petroleum reservoirs. Thus, the u...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-252
更新日期:2012-11-07 00:00:00
abstract:BACKGROUND:Susceptibility to Bordetella pertussis infection varies widely. These differences can partly be explained by genetic host factors. HcB-28 mice are more resistant to B. pertussis infection than C3H mice, which could partially be ascribed to the B. pertussis susceptibility locus-1 (Bps1) on chromosome 12. The ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-7-88
更新日期:2007-10-12 00:00:00
abstract:BACKGROUND:Fungi are the second most abundant type of human pathogens. Invasive fungal pathogens are leading causes of life-threatening infections in clinical settings. Toxicity to the host and drug-resistance are two major deleterious issues associated with existing antifungal agents. Increasing a host's tolerance and...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-224
更新日期:2013-10-07 00:00:00
abstract:BACKGROUND:Dispersal from Candida albicans biofilms that colonize catheters is implicated as a primary factor in the link between contaminated catheters and life threatening blood stream infections (BSI). Appropriate in vitro C. albicans biofilm models are needed to probe factors that induce detachment events. RESULTS...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-25
更新日期:2009-02-02 00:00:00
abstract:BACKGROUND:Next-generation sequencing platforms have revolutionised our ability to investigate the microbiota composition of complex environments, frequently through 16S rRNA gene sequencing of the bacterial component of the community. Numerous factors, including DNA extraction method, primer sequences and sequencing p...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0738-z
更新日期:2016-06-24 00:00:00
abstract:BACKGROUND:The date palm root borer Oryctes agamemnon (Coleoptera: Scarabaeidae) is one of the major pests of palms. In Saudi Arabia, both larvae and adults of Oryctes are particularly troublesome, especially during the establishment of young date palm orchards. Endosymbiotic bacteria are known to have a key role in fo...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0422-8
更新日期:2015-04-22 00:00:00
abstract:BACKGROUND:Mannheimia haemolytica strains isolated from North American cattle have been classified into two genotypes (1 and 2). Although members of both genotypes have been isolated from the upper and lower respiratory tracts of cattle with or without bovine respiratory disease (BRD), genotype 2 strains are much more ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01932-2
更新日期:2020-08-12 00:00:00
abstract:BACKGROUND:Fungi constitute an important yet frequently neglected component of the human microbiota with a possible role in health and disease. Fungi and bacteria colonise the infant gastrointestinal tract in parallel, yet most infant microbiome studies have ignored fungi. Milk is a source of diverse and viable bacteri...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01829-0
更新日期:2020-06-05 00:00:00
abstract:BACKGROUND:Ureaplasma urealyticum is a major pathogen associated with many diseases. The ability of U. urealyticum to protect itself from oxidative stress is likely to be important for its pathogenesis and survival, but its oxidative stress tolerance mechanisms remain unclear. This study investigates the antioxidant ac...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0485-6
更新日期:2015-07-26 00:00:00
abstract:BACKGROUND:Similar to Gram-negative bacteria, the outer membrane (OM) of the pathogenic spirochete, Borrelia burgdorferi, contains integral OM-spanning proteins (OMPs), as well as membrane-anchored lipoproteins. Although the mechanism of OMP biogenesis is still not well-understood, recent studies have indicated that a ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-60
更新日期:2012-04-20 00:00:00
abstract:BACKGROUND:Bacteria excrete costly toxins to defend their ecological niche. The evolution of such antagonistic interactions between individuals is expected to depend on both the social environment and the strength of resource competition. Antagonism is expected to be weak among highly similar genotypes because most ind...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-40
更新日期:2012-03-22 00:00:00