Abstract:
BACKGROUND:Klebsiella pneumoniae displaying the hypermucoviscosity (HV) phenotype are considered more virulent than HV-negative strains. Nevertheless, the emergence of tissue-abscesses-associated HV-negative isolates motivated us to re-evaluate the role of HV-phenotype. RESULTS:Instead of genetically manipulating the HV-phenotype of K. pneumoniae, we selected two clinically isolated K1 strains, 1112 (HV-positive) and 1084 (HV-negative), to avoid possible interference from defects in the capsule. These well-encapsulated strains with similar genetic backgrounds were used for comparative analysis of bacterial virulence in a pneumoniae or a liver abscess model generated in either naïve or diabetic mice. In the pneumonia model, the HV-positive strain 1112 proliferated to higher loads in the lungs and blood of naïve mice, but was less prone to disseminate into the blood of diabetic mice compared to the HV-negative strain 1084. In the liver abscess model, 1084 was as potent as 1112 in inducing liver abscesses in both the naïve and diabetic mice. The 1084-infected diabetic mice were more inclined to develop bacteremia and had a higher mortality rate than those infected by 1112. A mini-Tn5 mutant of 1112, isolated due to its loss of HV-phenotype, was avirulent to mice. CONCLUSION:These results indicate that the HV-phenotype is required for the virulence of the clinically isolated HV-positive strain 1112. The superior ability of the HV-negative stain 1084 over 1112 to cause bacteremia in diabetic mice suggests that factors other than the HV phenotype were required for the systemic dissemination of K. pneumoniae in an immunocompromised setting.
journal_name
BMC Microbioljournal_title
BMC microbiologyauthors
Lin YC,Lu MC,Tang HL,Liu HC,Chen CH,Liu KS,Lin C,Chiou CS,Chiang MK,Chen CM,Lai YCdoi
10.1186/1471-2180-11-50subject
Has Abstractpub_date
2011-03-08 00:00:00pages
50issn
1471-2180pii
1471-2180-11-50journal_volume
11pub_type
杂志文章相关文献
BMC MICROBIOLOGY文献大全abstract:BACKGROUND:In the present work, we describe a group of anomalous dose-response (DR) profiles and develop a dynamic model that is able to explain them. Responses were obtained from conventional assays of three antimicrobial agents (nisin, pediocin and phenol) against two microorganisms (Carnobacterium piscicola and Leuc...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-220
更新日期:2010-08-19 00:00:00
abstract:BACKGROUND:The 19 kDa lipoprotein of Mycobacterium tuberculosis (MTB) is an important target of the innate immune response. To investigate the effect of post-translation modification of this protein on innate recognition in the context of the whole bacillus, we derived a recombinant M. tuberculosis H37Rv that lacked th...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-93
更新日期:2009-05-14 00:00:00
abstract:BACKGROUND:The microbiological diagnosis of bacterial vaginosis is usually made using Nugent's criteria, a useful but rather laborious scoring system based on counting bacterial cell types on Gram stained slides of vaginal smears. Ison and Hay have simplified the score system to three categories and added a fourth cate...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-5-61
更新日期:2005-10-14 00:00:00
abstract:BACKGROUND:Hepatitis C virus (HCV) core protein and nonstructural protein 4B (NS4B) are potentially oncogenic. Aberrant activation of the Wnt/β-catenin signaling pathway is closely associated with hepatocarcinogenesis. We investigated the effects of HCV type 1b core protein and NS4B on Wnt/β-catenin signaling in variou...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-1032-4
更新日期:2017-05-25 00:00:00
abstract:BACKGROUND:Vibrio cholerae is the causative agent of cholera. Extensive studies reveal that complicated regulatory cascades regulate expression of virulence genes, the products of which are required for V. cholerae to colonize and cause disease. In this study, we investigated the expression of the key virulence regulat...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-3
更新日期:2010-01-06 00:00:00
abstract:BACKGROUND:Compared with many protists, Giardia lamblia has a simple life cycle alternating between cyst and trophozoite. Most research on the molecular biology of Giardia parasites has focused on trophozoites and the processes of excystation and encystation, whereas cysts have attracted less interest. The striking mor...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-11-91
更新日期:2011-05-04 00:00:00
abstract:BACKGROUND:Polycyclic aromatic hydrocarbons (PAHs), widespread pollutants in the marine environment, can produce adverse effects in marine organisms and can be transferred to humans through seafood. Our knowledge of PAH-degrading bacterial populations in the marine environment is still very limited, and mainly originat...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-50
更新日期:2008-03-25 00:00:00
abstract:BACKGROUND:In this study, we have evaluated the immunological status of hepatitis C virus (HCV)-infected patients aiming at identifying putative biomarkers associated with distinct degrees of liver fibrosis. Peripheral blood and tissue T-cells as well as cytokine levels were quantified by flow cytometry. RESULTS:Data ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0610-6
更新日期:2016-01-07 00:00:00
abstract:BACKGROUND:Probiotics are live microorganisms that, when administered in adequate amounts, confer a health benefit on the host, are now accepted as suitable alternatives to antibiotics in the control of animal infections and improving animal production. Lactic acid bacteria (LAB) with remarkable functional properties h...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1626-0
更新日期:2019-11-12 00:00:00
abstract:BACKGROUND:The human gastrointestinal (GI) tract microbiota is characterised by an abundance of uncultured bacteria most often assigned in phyla Firmicutes and Bacteroidetes. Diversity of this microbiota, even though approached with culture independent techniques in several studies, still requires more elucidation. The...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-68
更新日期:2009-04-08 00:00:00
abstract:BACKGROUND:After E. coli O157, E. coli O26 is the second most prevalent enterohaemorrhagic E. coli (EHEC) serotype identified in cases of foodborne illness in Australia and throughout the world. E. coli O26 associated foodborne outbreaks have drawn attention to the survival capabilities of this organism in a range of e...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-0963-0
更新日期:2017-03-01 00:00:00
abstract:BACKGROUND:The complete genome sequences of 44 Bacillus cereus group isolates collected from diverse sources in Japan were analyzed to determine their genetic backgrounds and diversity levels in Japan. Multilocus sequence typing (MLST) and core-genome single-nucleotide polymorphism (SNP) typing data from whole-genome s...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1678-1
更新日期:2019-12-16 00:00:00
abstract:BACKGROUND:The development of vaccines and evaluation of novel treatment strategies for invasive group A streptococcal (iGAS) disease requires suitable models of human infection that can be monitored longitudinally and are preferably non-invasive. Bio-photonic imaging provides an opportunity to reduce use of animals in...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1200-1
更新日期:2018-06-19 00:00:00
abstract:BACKGROUND:Cystic fibrosis (CF) lung disease has a unique profile of pathogens predominated by Pseudomonas aeruginosa (PsA) and Staphylococcus aureus (SA). These microorganisms must overcome host immune defense to colonize the CF lungs. Polymorphonuclear neutrophils are a major component of the host defense against bac...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-11-112
更新日期:2011-05-20 00:00:00
abstract:BACKGROUND:Alpha (alpha)-hemolysin is a pore forming cytolysin and serves as a virulence factor in intestinal and extraintestinal pathogenic strains of E. coli. It was suggested that the genes encoding alpha-hemolysin (hlyCABD) which can be found on the chromosome and plasmid, were acquired through horizontal gene tran...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-193
更新日期:2010-07-19 00:00:00
abstract:BACKGROUND:The aminoglycoside antibiotic gentamicin was supposed to induce a crosstalk between the Cpx- and the Arc-two-component systems (TCS). Here, we investigated the physical interaction of the respective TCS components and compared the results with their respective gene expression and protein abundance. The findi...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-1100-9
更新日期:2017-09-18 00:00:00
abstract:BACKGROUND:The ColRS two-component system has been shown to contribute to the membrane functionality and stress tolerance of Pseudomonas putida as well as to the virulence of Pseudomonas aeruginosa and plant pathogenic Xanthomonas species. However, the conditions activating the ColRS pathway and the signal(s) sensed by...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-14-162
更新日期:2014-06-20 00:00:00
abstract:BACKGROUND:Mob family proteins are conserved between animals, plants and fungi and are essential for the activation of NDR kinases that control crucial cellular processes like cytokinesis, proliferation and morphology. RESULTS:We identified a hypomorphic allele of ChMOB2 in a random insertional mutant (vir-88) of the ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-0932-7
更新日期:2017-01-19 00:00:00
abstract:BACKGROUND:Crohn's disease (CD) is a high morbidity chronic inflammatory disorder of unknown aetiology. Adherent-invasive Escherichia coli (AIEC) has been recently implicated in the origin and perpetuation of CD. Because bacterial biofilms in the gut mucosa are suspected to play a role in CD and biofilm formation is a ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-202
更新日期:2009-09-21 00:00:00
abstract:BACKGROUND:It is known that insects and crustaceans can carry simultaneous, active infections of two or more viruses without showing signs of disease, but it was not clear whether co-infecting viruses occupied the same cells or different cells in common target tissues. Our previous work showed that successive challenge...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-14
更新日期:2010-01-20 00:00:00
abstract:BACKGROUND:Mycoplasmas-contamination of Orientia tsutsugamushi, one of the obligated intracellular bacteria, is a very serious problem in in vitro studies using cell cultures because mycoplasmas have significant influence on the results of scientific studies. Only a recommended decontamination method is to passage the ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-32
更新日期:2013-02-08 00:00:00
abstract:BACKGROUND:Over the last years oleaginous yeasts have been studied for several energetic, oleochemical, medical and pharmaceutical purposes. However, only a small number of yeasts are known and have been deeply exploited. The search for new isolates with high oleaginous capacity becomes imperative, as well as the use o...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01742-6
更新日期:2020-03-14 00:00:00
abstract:BACKGROUND:Methicillin-resistant Staphylococcus aureus (MRSA) is known as a leading cause of morbidity and mortality. Investigation of the MRSA's virulence and resistance mechanisms is a continuing concern toward controlling such burdens through using high throughput whole Genome Sequencing (WGS) and molecular diagnost...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1470-2
更新日期:2019-06-11 00:00:00
abstract:BACKGROUND:Photorhabdus are Gram-negative nematode-symbiotic and insect-pathogenic bacteria. The species Photorhabdus asymbiotica is able to infect humans as well as insects. We investigated the secreted proteome of a clinical isolate of P. asymbiotica at different temperatures in order to identify proteins relevant to...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-141
更新日期:2010-05-12 00:00:00
abstract:BACKGROUND:The sequenced O45:K1:H7 Escherichia coli meningitis strain S88 harbors a large virulence plasmid. To identify possible genetic determinants of pS88 virulence, we examined the transcriptomes of 88 plasmidic ORFs corresponding to known and putative virulence genes, and 35 ORFs of unknown function. RESULTS:Qua...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-115
更新日期:2012-06-21 00:00:00
abstract:BACKGROUND:The human gastrointestinal (GI) tract microbiota has been a subject of intense research throughout the 3rd Millennium. Now that a general picture about microbiota composition in health and disease is emerging, questions about factors determining development of microbiotas with specific community structures w...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1538-z
更新日期:2019-07-18 00:00:00
abstract:BACKGROUND:Porphyromonas gingivalis is the foremost oral pathogen of adult periodontitis in humans. However, the mechanisms of bacterial invasion and the resultant destruction of the gingival tissue remain largely undefined. RESULTS:We report host-P. gingivalis interactions in primary human gingival fibroblast (HGF) c...
journal_title:BMC microbiology
pub_type: 杂志文章,收录出版
doi:10.1186/1471-2180-6-26
更新日期:2006-03-08 00:00:00
abstract:BACKGROUND:Salmonella enterica and Campylobacter jejuni are amongst the more prevalent bacterial pathogens that cause foodborne diseases. These microorganisms are common contaminants of poultry and poultry products. This study was aimed to evaluate the antibacterial activity of metallic copper surfaces on these importa...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-4-19
更新日期:2004-04-30 00:00:00
abstract:BACKGROUND:Candida krusei has been known to exhibit communal interactions such as pellicle formation and crawling out of nutritional broth. We noticed another possible interaction on agar surfaces, where C. krusei yeast cells formed mycelia along agar surfaces toward each other. We report here the results of experiment...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-0972-z
更新日期:2017-03-11 00:00:00
abstract:BACKGROUND:Whole-genome sequencing using high throughput technologies has revolutionized and speeded up the scientific investigation of bacterial genetics, biochemistry, and molecular biology. Lactic acid bacteria (LABs) have been extensively used in fermentation and more recently as probiotics in food products that pr...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01980-8
更新日期:2020-10-23 00:00:00