Abstract:
BACKGROUND:The ColRS two-component system has been shown to contribute to the membrane functionality and stress tolerance of Pseudomonas putida as well as to the virulence of Pseudomonas aeruginosa and plant pathogenic Xanthomonas species. However, the conditions activating the ColRS pathway and the signal(s) sensed by ColS have remained unknown. Here we aimed to analyze the role of the ColRS system in metal tolerance of P. putida and to test whether ColS can respond to metal excess. RESULTS:We show that the ColRS system is necessary for P. putida to tolerate the excess of iron and zinc, and that it also contributes to manganese and cadmium tolerance. Excess of iron, zinc, manganese or cadmium activates ColRS signaling and as a result modifies the expression of ColR-regulated genes. Our data suggest that the genes in the ColR regulon are functionally redundant, as several loci have to be deleted to observe a significant decrease in metal tolerance. Site-directed mutagenesis of ColS revealed that excess of iron and, surprisingly, also zinc are sensed by a conserved ExxE motif in ColS's periplasmic domain. While ColS is able to sense different metals, it still discriminates between the two oxidation states of iron, specifically responding to ferric and not ferrous iron. We propose a signal perception model involving a dimeric ColS, where each monomer donates one ExxE motif for metal binding. CONCLUSIONS:Several transition metals are essential for living organisms in certain amounts, but toxic in excess. We show that ColRS is a sensor system which detects and responds to the excess of physiologically important metals such as zinc, iron and manganese. Thus, the ColRS system is an important factor for metal homeostasis and tolerance in P. putida.
journal_name
BMC Microbioljournal_title
BMC microbiologyauthors
Ainsaar K,Mumm K,Ilves H,Hõrak Rdoi
10.1186/1471-2180-14-162subject
Has Abstractpub_date
2014-06-20 00:00:00pages
162issn
1471-2180pii
1471-2180-14-162journal_volume
14pub_type
杂志文章相关文献
BMC MICROBIOLOGY文献大全abstract:BACKGROUND:The opportunistic pathogen, Pseudomonas aeruginosa is well known for its environmental and metabolic versatility, yet many of the functions of its gene-products remain to be fully elucidated. This study's objective was to illuminate the potential functions of under-described gene-products during the medicall...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1441-7
更新日期:2019-04-01 00:00:00
abstract:BACKGROUND:Enterohemorrhagic Escherichia coli (EHEC) colonizes the intestinal epithelium and causes attaching and effacing (A/E) lesions. Expression of virulence genes, particularly those from the locus of the enterocyte effacement (LEE) pathogenicity island is required for the formation of a type three secretion syste...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-231
更新日期:2012-10-11 00:00:00
abstract:BACKGROUND:Persister cells comprise a phenotypic variant that shows extreme antibiotic tolerance resulting in treatment failures of bacterial infections. While this phenomenon has posed a great threat in public health, mechanisms underlying their formation in Staphylococcus aureus remain largely unknown. Increasing evi...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-1129-9
更新日期:2017-11-22 00:00:00
abstract:BACKGROUND:Microbial fuel cells (MFCs) are devices that exploit microorganisms to generate electric power from organic matter. Despite the development of efficient MFC reactors, the microbiology of electricity generation remains to be sufficiently understood. RESULTS:A laboratory-scale two-chamber microbial fuel cell ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-6
更新日期:2008-01-10 00:00:00
abstract:BACKGROUND:Previous phylogenetic analyses of species within the genus Golovinomyces (Ascomycota, Erysiphales), based on ITS and 28S rDNA sequence data, revealed a co-evolutionary relationship between powdery mildew species and hosts of certain tribes of the plant family Asteraceae. Golovinomyces growing on host plants ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01731-9
更新日期:2020-03-05 00:00:00
abstract:BACKGROUND:Globally, staphylococci have developed resistance to many antibiotics. New approaches to chemotherapy are needed and one such approach could be to use plant derived actives with conventional antibiotics in a synergestic way. The purpose of this study was to isolate α-mangostin from the mangosteen (Garcinia m...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0814-4
更新日期:2016-08-26 00:00:00
abstract:BACKGROUND:Recent studies suggest that the reported protective effects of statins (HMG-CoA reductase inhibitors) against community-acquired pneumonia (CAP) and sepsis in humans may be due to confounders and a healthy user-effect. To directly test whether statins are protective against Streptococcus pneumoniae, the lead...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-73
更新日期:2012-05-15 00:00:00
abstract:BACKGROUND:Probiotics have been reported to reduce total cholesterol levels in vitro, but more evidence is needed to determine the clinical relevance of this activity. Chinese traditional fermented pickles are a good source of lactic acid bacteria. Therefore, pickle samples were collected for screening lactic acid bact...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01922-4
更新日期:2020-08-04 00:00:00
abstract:BACKGROUND:Coxiella burnetii is a Gram-negative intracellular bacterial pathogen that replicates within a phagolysosome-like parasitophorous vacuole (PV) of macrophages. PV formation requires delivery of effector proteins directly into the host cell cytoplasm by a type IVB secretion system. However, additional secretio...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-222
更新日期:2013-10-05 00:00:00
abstract:BACKGROUND:Many bacterial extracytoplasmic proteins are stabilized by intramolecular disulfide bridges that are formed post-translationally between their cysteine residues. This protein modification plays an important role in bacterial pathogenesis, and is facilitated by the Dsb (disulfide bond) family of the redox pro...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-11-166
更新日期:2011-07-25 00:00:00
abstract:BACKGROUND:The low bacterial load in samples acquired from the lungs, have made studies on the airway microbiome vulnerable to contamination from bacterial DNA introduced during sampling and laboratory processing. We have examined the impact of laboratory contamination on samples collected from the lower airways by pro...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1560-1
更新日期:2019-08-14 00:00:00
abstract:BACKGROUND:After E. coli O157, E. coli O26 is the second most prevalent enterohaemorrhagic E. coli (EHEC) serotype identified in cases of foodborne illness in Australia and throughout the world. E. coli O26 associated foodborne outbreaks have drawn attention to the survival capabilities of this organism in a range of e...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-0963-0
更新日期:2017-03-01 00:00:00
abstract:BACKGROUND:Poorly understood but highly diverse microbial communities exist within anoxic and oxygen-depleted marine sediments. These communities often harbour single-celled eukaryotes that form symbiotic associations with different prokaryotes. During low tides in South-western British Columbia, Canada, vast areas of ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-145
更新日期:2010-05-19 00:00:00
abstract:BACKGROUND:Legionella testing conducted at environmental laboratories plays an essential role in assessing the risk of disease transmission associated with water systems. However, drawbacks of culture-based methodology used for Legionella enumeration can have great impact on the results and interpretation which togethe...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0423-7
更新日期:2015-04-29 00:00:00
abstract:BACKGROUND:Vibrio anguillarum is an extracellular bacterial pathogen that is a causative agent of vibriosis in finfish and crustaceans with mortality rates ranging from 30% to 100%. Mutations in central metabolism (glycolysis and the TCA cycle) of intracellular pathogens often result in attenuated virulence due to depl...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-1124-1
更新日期:2017-11-14 00:00:00
abstract:BACKGROUND:Global warming has triggered an increase in the prevalence and severity of coral disease, yet little is known about coral/pathogen interactions in the early stages of infection. The point of entry of the pathogen and the route that they take once inside the polyp is currently unknown, as is the coral's capac...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1173-0
更新日期:2018-04-20 00:00:00
abstract:BACKGROUND:In the present work, we describe a group of anomalous dose-response (DR) profiles and develop a dynamic model that is able to explain them. Responses were obtained from conventional assays of three antimicrobial agents (nisin, pediocin and phenol) against two microorganisms (Carnobacterium piscicola and Leuc...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-220
更新日期:2010-08-19 00:00:00
abstract:BACKGROUND:Clostridium thermocellum is a Gram-positive thermophilic anaerobic bacterium with the unusual capacity to convert cellulosic biomass into ethanol and hydrogen. Identification and characterization of protein complexes in C. thermocellum are important toward understanding its metabolism and physiology. RESULT...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-11-22
更新日期:2011-01-26 00:00:00
abstract:BACKGROUND:Listeria monocytogenes has been implicated in several food borne outbreaks as well as sporadic cases of disease. Increased understanding of the biology of this organism is important in the prevention of food borne listeriosis. The infectivity of Listeria monocytogenes ScottA, cultivated with and without oxyg...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-7-55
更新日期:2007-06-14 00:00:00
abstract:BACKGROUND:The plasma membrane plays an essential role in selective permeability, compartmentalization, osmotic balance, and cellular uptake. The characteristics and functions of cyanobacterial membranes have been extensively investigated in recent years. Cell-penetrating peptides (CPPs) are special nanocarriers that c...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-57
更新日期:2013-03-14 00:00:00
abstract:BACKGROUND:Viability staining with SYTO9 and propidium iodide (PI) is a frequently used tool in microbiological studies. However, data generated by such routinely used method are often not critically evaluated for their accuracy. In this study we aim to investigate the critical aspects of this staining method using Sta...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0376-x
更新日期:2015-02-18 00:00:00
abstract:BACKGROUND:Heterologous prime-boost immunization protocols using different gene expression systems have proven to be successful tools in protecting against various diseases in experimental animal models. The main reason for using this approach is to exploit the ability of expression cassettes to prime or boost the immu...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-5-45
更新日期:2005-08-02 00:00:00
abstract:BACKGROUND:Neisseria meningitidis serogroups W and Y are the most common serogroups causing invasive meningococcal disease in Sweden. The majority of cases are caused by the serogroup W UK 2013 strain of clonal complex (cc) 11, and subtype 1 of the serogroup Y, YI strain of cc23. In this study, virulence factors of sev...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01760-4
更新日期:2020-04-15 00:00:00
abstract:BACKGROUND:Iron is an essential nutrient but can be toxic at high intracellular concentrations and organisms have evolved tightly regulated mechanisms for iron uptake and homeostasis. Information on iron management mechanisms is available for organisms living at circumneutral pH. However, very little is known about how...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-203
更新日期:2008-11-24 00:00:00
abstract:BACKGROUND:Xanthophyllomyces dendrorhous is a basidiomycetous yeast that synthesizes astaxanthin, a carotenoid with great biotechnological impact. The ergosterol and carotenoid synthetic pathways derive from the mevalonate pathway and involve cytochrome P450 enzymes. Among these enzymes, the CYP51 family, which is invo...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0428-2
更新日期:2015-04-25 00:00:00
abstract:BACKGROUND:Ralstonia eutropha (syn. Cupriavidus necator) is a model microorganism for studying metabolism of polyhydroxyalkanoates (PHAs) and a potential chassis for protein expression due to various advantages. Although current plasmid systems of R. eutropha provide a basic platform for gene expression, the performanc...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01812-9
更新日期:2020-05-19 00:00:00
abstract:BACKGROUND:Next-generation sequencing platforms have revolutionised our ability to investigate the microbiota composition of complex environments, frequently through 16S rRNA gene sequencing of the bacterial component of the community. Numerous factors, including DNA extraction method, primer sequences and sequencing p...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0738-z
更新日期:2016-06-24 00:00:00
abstract:BACKGROUND:It is unclear how DNA is packaged in a bacterial cell in the absence of nucleosomes. To investigate the initial level of DNA condensation in bacterial nucleoid we used in vivo DNA digestion coupled with high-throughput sequencing of the digestion-resistant fragments. To this end, we transformed E. coli cells...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0637-3
更新日期:2016-02-20 00:00:00
abstract:BACKGROUND:Antimicrobial helper-compounds may reverse antimicrobial resistance. Sertraline, a antidepressant drug, has been suggested as a tetracycline helper-compound. Tetracycline is the preferred antimicrobial for treatment of enteric diseases in pigs. This study is the first to evaluate the potency of sertraline as...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1383-5
更新日期:2019-01-11 00:00:00
abstract:BACKGROUND:The ATP-Binding Cassette (ABC) functional superfamily includes integral transmembrane exporters that have evolved three times independently, forming three families termed ABC1, ABC2 and ABC3, upon which monophyletic ATPases have been superimposed for energy-coupling purposes [e.g., J Membr Biol 231(1):1-10, ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-98
更新日期:2013-05-06 00:00:00