Abstract:
BACKGROUND:The human gastrointestinal (GI) tract microbiota has been a subject of intense research throughout the 3rd Millennium. Now that a general picture about microbiota composition in health and disease is emerging, questions about factors determining development of microbiotas with specific community structures will be addressed. To this end, usage of murine models for colonization studies remains crucial. Optical in vivo imaging of either bioluminescent or fluorescent bacteria is the basis for non-invasive detection of intestinal colonization of bacteria. Although recent advances in in vivo fluorescence imaging have overcome many limitations encountered in bioluminescent imaging of intestinal bacteria, such as requirement for live cells, high signal attenuation and 2D imaging, the method is still restricted to bacteria for which molecular cloning tools are available. RESULTS:Here, we present usage of a lipophilic fluorescent dye together with Katushka far-red fluorescent protein to establish a dual-color in vivo imaging system to monitor GI transit of different bacterial strains, suitable also for strains resistant to genetic labeling. Using this system, we were able to distinguish two different E. coli strains simultaneously and show their unique transit patterns. Combined with fluorescence molecular tomography, these distinct strains could be spatially and temporally resolved and quantified in 3D. CONCLUSIONS:Developed novel method for labeling microbes and identify their passage both temporally and spatially in vivo makes now possible to monitor all culturable bacterial strains, also those that are resistant to conventional genetic labeling.
journal_name
BMC Microbioljournal_title
BMC microbiologyauthors
Peñate-Medina O,Tower RJ,Peñate-Medina T,Will O,Saris PEJ,Suojanen J,Sorsa T,Huuskonen L,Hiippala K,Satokari R,Glüer CC,de Vos WM,Reunanen Jdoi
10.1186/s12866-019-1538-zsubject
Has Abstractpub_date
2019-07-18 00:00:00pages
167issue
1issn
1471-2180pii
10.1186/s12866-019-1538-zjournal_volume
19pub_type
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