Abstract:
BACKGROUND:Accurate identification of Aspergillus species is a very important subject. Mass spectral fingerprinting using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is generally employed for the rapid identification of fungal isolates. However, the results are based on simple mass spectral pattern-matching, with no peak assignment and no taxonomic input. We propose here a ribosomal subunit protein (RSP) typing technique using MALDI-TOF MS for the identification and discrimination of Aspergillus species. The results are concluded to be phylogenetic in that they reflect the molecular evolution of housekeeping RSPs. RESULTS:The amino acid sequences of RSPs of genome-sequenced strains of Aspergillus species were first verified and compared to compile a reliable biomarker list for the identification of Aspergillus species. In this process, we revealed that many amino acid sequences of RSPs (about 10-60%, depending on strain) registered in the public protein databases needed to be corrected or newly added. The verified RSPs were allocated to RSP types based on their mass. Peak assignments of RSPs of each sample strain as observed by MALDI-TOF MS were then performed to set RSP type profiles, which were then further processed by means of cluster analysis. The resulting dendrogram based on RSP types showed a relatively good concordance with the tree based on β-tubulin gene sequences. RSP typing was able to further discriminate the strains belonging to Aspergillus section Fumigati. CONCLUSIONS:The RSP typing method could be applied to identify Aspergillus species, even for species within section Fumigati. The discrimination power of RSP typing appears to be comparable to conventional β-tubulin gene analysis. This method would therefore be suitable for species identification and discrimination at the strain to species level. Because RSP typing can characterize the strains within section Fumigati, this method has potential as a powerful and reliable tool in the field of clinical microbiology.
journal_name
BMC Microbioljournal_title
BMC microbiologyauthors
Nakamura S,Sato H,Tanaka R,Kusuya Y,Takahashi H,Yaguchi Tdoi
10.1186/s12866-017-1009-3subject
Has Abstractpub_date
2017-04-26 00:00:00pages
100issue
1issn
1471-2180pii
10.1186/s12866-017-1009-3journal_volume
17pub_type
杂志文章相关文献
BMC MICROBIOLOGY文献大全abstract:BACKGROUND:Lyme borrelia genotypes differ in their capacity to cause disseminated disease. Gene array analysis was employed to profile the host transcriptome induced by Borrelia burgdorferi strains with different capacities for causing disseminated disease in the blood of C3H/HeJ mice during early infection. RESULTS:B...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0644-4
更新日期:2016-03-08 00:00:00
abstract:BACKGROUND:The gene encoding the inorganic pyrophosphatase (PPase) of the intracellular pathogen Legionella pneumophila is induced during intracellular infection, but is constitutively expressed in Escherichia coli. The causative agent of tuberculosis, Mycobacterium tuberculosis, contains a well conserved copy of PPase...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-1-3
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Mycobacterium avium is an environmental mycobacterium that can be divided into the subspecies avium, hominissuis, paratuberculosis and silvaticum. Some M. avium subspecies are opportunistic pathogens for animals and humans. They are ubiquitous in nature and can be isolated from natural sources of water, soil...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-7-14
更新日期:2007-03-05 00:00:00
abstract:BACKGROUND:It is unclear how DNA is packaged in a bacterial cell in the absence of nucleosomes. To investigate the initial level of DNA condensation in bacterial nucleoid we used in vivo DNA digestion coupled with high-throughput sequencing of the digestion-resistant fragments. To this end, we transformed E. coli cells...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0637-3
更新日期:2016-02-20 00:00:00
abstract:BACKGROUND:Salmonella enterica is a causative agent of foodborne gastroenteritis and the systemic disease known as typhoid fever. This bacterium uses two type three secretion systems (T3SSs) to translocate protein effectors into host cells to manipulate cellular function. Salmonella pathogenicity island (SPI)-2 encodes...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-221
更新日期:2013-10-04 00:00:00
abstract:BACKGROUND:The Beijing lineage of Mycobacterium tuberculosis is causing concern due to its global distribution and its involvement in severe outbreaks. Studies focused on this lineage are mainly restricted to geographical settings where its prevalence is high, whereas those in other areas are scarce. In this study, we ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-151
更新日期:2010-05-25 00:00:00
abstract:BACKGROUND:Cow manure is not only an agricultural waste, but also an organic fertilizer resource. The application of organic fertilizer is a feasible practice to mitigate the soil degradation caused by overuse of chemical fertilizers, which can affect the bacterial diversity and community composition in soils. However,...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01871-y
更新日期:2020-07-01 00:00:00
abstract:BACKGROUND:The native microflora associated with mosquitoes have important roles in mosquito development and vector competence. Sequencing of bacterial V3 region from 16S rRNA genes across the developmental stages of Culex mosquitoes (early and late larval instars, pupae and adults) was used to test the hypothesis that...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0475-8
更新日期:2015-07-24 00:00:00
abstract:BACKGROUND:Bacteria employ multiple mechanisms to control gene expression and react to their constantly changing environment. Bacterial growth in rich laboratory medium is a dynamic process in which bacteria utilize nutrients from simple to complex and change physical properties of the medium, as pH, during the process...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-32
更新日期:2009-02-10 00:00:00
abstract:BACKGROUND:Nontuberculous mycobacteria (NTM) are ubiquitous organisms and the incidence of NTM infections has been increasing in recent years. Mycobacteroides abscessus (M. abscessus) is one of the most antimicrobial-resistant NTM; however, no reliable antibiotic regimen can be officially advocated. We evaluated the ef...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-02000-5
更新日期:2020-10-19 00:00:00
abstract:BACKGROUND:Non typhoidal salmonellosis is one of the neglected zoonoses in most African countries. The use of sub-therapeutic doses of antibiotics as animal growth promoter enhances the emergence and dissemination of antimicrobial resistance in bacteria with food animal reservoirs and may also results in antibiotics re...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1248-y
更新日期:2018-08-30 00:00:00
abstract:BACKGROUND:The mce operons play an important role in the entry of M. tuberculosis into macrophages and non-phagocytic cells. Their non-redundant function as well as complex regulation is implied by the phenotype of mce mutants. Recently, mce1 operon was found to extend over 13 genes, fadD5 (Rv0166) being the first gene...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-128
更新日期:2010-04-27 00:00:00
abstract:BACKGROUND:The lung of patients with cystic fibrosis (CF) is particularly sensitive to Pseudomonas aeruginosa. This bacterium plays an important role in the poor outcome of CF patients. During the disease progress, first acquisition of P. aeruginosa is the key-step in the management of CF patients. Quantitative PCR (qP...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-143
更新日期:2013-06-21 00:00:00
abstract:BACKGROUND:Emergence of drug resistant varieties of tuberculosis is posing a major threat to global tuberculosis eradication programmes. Although several approaches have been explored to counter resistance, there has been limited success due to a lack of understanding of how resistance emerges in bacteria upon drug tre...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-234
更新日期:2008-12-23 00:00:00
abstract:BACKGROUND:Fusobacterium nucleatum, a well studied bacterium in periodontal diseases, appendicitis, gingivitis, osteomyelitis and pregnancy complications has recently gained attention due to its association with colorectal cancer (CRC) progression. Treatment with berberine was shown to reverse F. nucleatum-induced CRC ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0700-0
更新日期:2016-05-12 00:00:00
abstract:BACKGROUND:Chlamydia pneumoniae is a common human pathogen that is associated with upper and lower respiratory tract infections. It has also been suggested that C. pneumoniae infection can trigger or promote a number of chronic inflammatory conditions, including asthma and atherosclerosis. Several strains of C. pneumon...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0569-3
更新日期:2015-10-23 00:00:00
abstract:BACKGROUND:Bacillus sp. H2O-1, isolated from the connate water of a Brazilian reservoir, produces an antimicrobial substance (denoted as AMS H2O-1) that is active against sulfate reducing bacteria, which are the major bacterial group responsible for biogenic souring and biocorrosion in petroleum reservoirs. Thus, the u...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-252
更新日期:2012-11-07 00:00:00
abstract:BACKGROUND:The increasing availability of whole genome sequences allows the gene or protein content of different organisms to be compared, leading to burgeoning interest in the relatively new subfield of pan-genomics. However, while several studies have analyzed protein content relationships in specific groups of bacte...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-258
更新日期:2010-10-13 00:00:00
abstract:BACKGROUND:Mycoplasmas-contamination of Orientia tsutsugamushi, one of the obligated intracellular bacteria, is a very serious problem in in vitro studies using cell cultures because mycoplasmas have significant influence on the results of scientific studies. Only a recommended decontamination method is to passage the ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-32
更新日期:2013-02-08 00:00:00
abstract:BACKGROUND:Chlortetracycline (CTC) is one of the commercially important tetracyclines (TCs) family product and is mainly produced by Streptomyces. CTC is still in a great demand due to its broad-spectrum activity against pathogens. Engineering transcriptional control allows the cell to allocate its valuable resources t...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1670-9
更新日期:2019-12-10 00:00:00
abstract:BACKGROUND:The increase in resistance to antibiotics among disease-causing bacteria necessitates the development of alternative antimicrobial approaches such as the use of light-activated antimicrobial agents (LAAAs). Light of an appropriate wavelength activates the LAAA to produce cytotoxic species which can then caus...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-111
更新日期:2008-07-01 00:00:00
abstract:BACKGROUND:Staphylococcus aureus (S. aureus) is one of the primary causes of bone infections which are often chronic and difficult to eradicate. Bacteria like S. aureus may survive upon internalization in cells and may be responsible for chronic and recurrent infections. In this study, we compared the responses of a ph...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-014-0207-5
更新日期:2014-07-25 00:00:00
abstract:BACKGROUND:Avian pathogenic Escherichia coli (APEC) and uropathogenic E. coli (UPEC) are the two main subsets of extraintestinal pathogenic E. coli (ExPEC). Both types have multiple iron acquisition systems, including heme and siderophores. Although iron transport systems involved in the pathogenesis of APEC or UPEC ha...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-143
更新日期:2012-07-20 00:00:00
abstract:BACKGROUND:Despite the high prevalence of genotype 1b hepatitis C virus (HCV) among patients, a cell culture system that permits entire viral life cycle of genotype 1b isolates is limited. To develop a cell-cultured hepatitis C virus (HCVcc) of genotype 1b, the proper combination of HCV genomic variants and host cells ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0846-9
更新日期:2016-09-27 00:00:00
abstract:BACKGROUND:The resurgence of multi-drug resistant tuberculosis (MDR-TB) and HIV associated tuberculosis (TB) are of serious global concern. To contain this situation, new anti-tuberculosis drugs and reduced treatment regimens are imperative. Recently, a nitroimidazole, PA-824, has been shown to be active against both r...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-218
更新日期:2013-10-01 00:00:00
abstract:BACKGROUND:Bacteria adapted to live within animals can protect their hosts against harmful infections. Beyond antagonism with pathogens, a 'defensive' bacterial symbiont could engage in additional interactions with other colonizing micro-organisms. A single bacterium might thus have cascading ecological impacts on the ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01845-0
更新日期:2020-06-15 00:00:00
abstract:BACKGROUND:Bacteria excrete costly toxins to defend their ecological niche. The evolution of such antagonistic interactions between individuals is expected to depend on both the social environment and the strength of resource competition. Antagonism is expected to be weak among highly similar genotypes because most ind...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-40
更新日期:2012-03-22 00:00:00
abstract:BACKGROUND:Staphylococcus aureus methicillin-resistant (MRSA) has been frequently isolated from endotracheal and lung puncture aspirates in malnourished children with pneumonia. In this work we evaluated the susceptibility of undernourished BALB/c mice and its ability to mount a protective immunity against MRSA with em...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-240
更新日期:2009-11-23 00:00:00
abstract:BACKGROUND:Thiomonas strains are ubiquitous in arsenic-contaminated environments. Differences between Thiomonas strains in the way they have adapted and respond to arsenic have never been studied in detail. For this purpose, five Thiomonas strains, that are interesting in terms of arsenic metabolism were selected: T. a...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-127
更新日期:2009-06-23 00:00:00
abstract:BACKGROUND:Bacillus mycoides Flügge, a Gram-positive, non-motile soil bacterium assigned to Bacillus cereus group, grows on agar as chains of cells linked end to end, forming radial filaments curving clock- or counter-clockwise (SIN or DX morphotypes). The molecular mechanism causing asymmetric curving is not known: ou...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-2-33
更新日期:2002-11-13 00:00:00