Abstract:
BACKGROUND:Escherichia coli is a well-studied anaerobic bacteria which is able to regulate metabolic pathways depending on the type of sugar presented in the medium. We have studied the glucose-lactose shift in E. coli at the protein level using a recently developed mass spectrometry platform. METHOD:Cells were grown in minimal medium containing two sugars (glucose and lactose) and analyzed using novel mass spectrometry cluster. The cluster combines the high resolving power and dynamic range of Fourier transform ion cyclotron resonance (FTICR) for accurate mass measurement and quantitation with multiple ion traps for fast and sensitive tandem mass spectrometry. The protein expression profile was followed in time across the glucose-lactose diauxic shift using label-free quantitation from the FTICR data. RESULTS AND CONCLUSION:The entire dataset was interrogated by KEGG pathway analysis, mapping measured changes in protein abundance onto known metabolic pathways. The obtained results were consistent with previously published gene expression data, with β-galactosidase being the most strongly induced protein during the diauxic shift.
journal_name
BMC Microbioljournal_title
BMC microbiologyauthors
Mostovenko E,Deelder AM,Palmblad Mdoi
10.1186/1471-2180-11-126subject
Has Abstractpub_date
2011-06-01 00:00:00pages
126issn
1471-2180pii
1471-2180-11-126journal_volume
11pub_type
杂志文章相关文献
BMC MICROBIOLOGY文献大全abstract:BACKGROUND:Thiomonas strains are ubiquitous in arsenic-contaminated environments. Differences between Thiomonas strains in the way they have adapted and respond to arsenic have never been studied in detail. For this purpose, five Thiomonas strains, that are interesting in terms of arsenic metabolism were selected: T. a...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-127
更新日期:2009-06-23 00:00:00
abstract:BACKGROUND:Helicobacter pylori has diverged in parallel to its human host, leading to distinct phylogeographic populations. Recent evidence suggests that in the current human mixing in Latin America, European H. pylori (hpEurope) are increasingly dominant at the expense of Amerindian haplotypes (hspAmerind). This pheno...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-211
更新日期:2013-09-19 00:00:00
abstract:BACKGROUND:Glycopeptidolipids (GPLs) are among the major free glycolipid components of the outer membrane of several saprophytic and clinically-relevant Mycobacterium species. The architecture of GPLs is based on a constant tripeptide-amino alcohol core of nonribosomal peptide synthetase origin that is N-acylated with ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-118
更新日期:2012-06-22 00:00:00
abstract:BACKGROUND:Acinetobacter baumannii is a gram-negative bacterium which causes opportunistic infections in immunocompromised hosts. Genome plasticity has given rise to a wide range of strain variation with respect to antimicrobial resistance profiles and expression of virulence factors which lead to altered phenotypes as...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-02082-1
更新日期:2021-01-09 00:00:00
abstract:BACKGROUND:Mob family proteins are conserved between animals, plants and fungi and are essential for the activation of NDR kinases that control crucial cellular processes like cytokinesis, proliferation and morphology. RESULTS:We identified a hypomorphic allele of ChMOB2 in a random insertional mutant (vir-88) of the ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-017-0932-7
更新日期:2017-01-19 00:00:00
abstract:BACKGROUND:Bacterial and cellular genotyping is becoming increasingly important in the diagnosis of infectious diseases. However, difficulties in obtaining sufficient amount of bacterial and cellular DNA extracted from the same human biopsy specimens is often a limiting factor. In this study, total DNA (host and bacter...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-175
更新日期:2008-10-08 00:00:00
abstract:BACKGROUND:Microencapsulated organic acids and botanicals have the potential to develop into important tools for the poultry industry. A blend of organic acids and botanicals (AviPlus®P) has previously shown to reduce Salmonella and Campylobacter in chickens; however, changes to the microbiota of the jejunum and ileum ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-02001-4
更新日期:2020-11-02 00:00:00
abstract:BACKGROUND:Campylobacter jejuni is the predominant cause of antecedent infection in post-infectious neuropathies such as the Guillain-Barré (GBS) and Miller Fisher syndromes (MFS). GBS and MFS are probably induced by molecular mimicry between human gangliosides and bacterial lipo-oligosaccharides (LOS). This study desc...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-6-32
更新日期:2006-04-04 00:00:00
abstract:BACKGROUND:Cryptosporidium spp. and Giardia duodenalis are important causes of diarrheal diseases in humans and animals worldwide, and there is an increased interest in the role of animals in the mechanical transmission of these protozoa. To examine the role of yaks in this process, we examined the occurrence and genot...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0446-0
更新日期:2015-05-21 00:00:00
abstract:BACKGROUND:Several conventional methods, including fungal culture and periodic acid-Schiff (PAS) reagent staining, have been used to diagnose oral candidiasis. The aim of this study was to evaluate the efficacy of a novel method, fungal fluorescent staining, in relation to conventional protocols in the diagnosis of ora...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1467-x
更新日期:2019-05-14 00:00:00
abstract:BACKGROUND:Streptococcus pneumoniae is a common respiratory pathogen and a major causative agent of respiratory infections, including otitis media (OM). Pneumococcal biofilms have been demonstrated on biopsies of the middle ear mucosa in children receiving tympanostomy tubes, supporting the hypothesis that chronic OM m...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-173
更新日期:2008-10-08 00:00:00
abstract:BACKGROUND:Microbial biofilms are known to cause an increasing number of chronic inflammatory and infectious conditions. A classical example is chronic periodontal disease, a condition initiated by the subgingival dental plaque biofilm on gingival epithelial tissues. We describe here a new model that permits the examin...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-280
更新日期:2009-12-31 00:00:00
abstract:BACKGROUND:Most of the studies evaluating the secular trends of blood isolates come from tertiary hospitals in urban areas. We sought to study the trends of the antimicrobial resistance of blood isolates in patients from a rural population hospitalized in a tertiary hospital in a small city in Greece. METHODS:We retro...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-6-41
更新日期:2006-05-02 00:00:00
abstract:BACKGROUND:Fluconazole (FLC), a triazole antifungal drug, is widely used for the maintenance therapy of cryptococcal meningoencephalitis, the most common opportunistic infection in AIDS patients. In this study, we examined changes in the gene expression profile of the C. neoformans reference strain H99 (serotype A) fol...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-11-97
更新日期:2011-05-11 00:00:00
abstract:BACKGROUND:HSV-1 is a common pathogen that infects 50-90% of the human population worldwide. HSV-1 causes numerous infection-related diseases, some of which are severely life-threatening. There are antiviral medications with activity against HSV-1. However, with the emergence of drug-resistant mutant strains of HSV-1, ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1639-8
更新日期:2019-12-07 00:00:00
abstract:BACKGROUND:Tetrahymena thermophila is one of the best characterized unicellular eukaryotes and its genome is sequenced in its entirety. However, the AT-richness of the genome and an unusual codon usage cause problems in cloning and expression of the ciliate DNA. To overcome these technical hiatuses we developed a Cre-d...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-7-12
更新日期:2007-03-01 00:00:00
abstract:BACKGROUND:The aim of this study was to identify mutations of rpoB, katG, inhA and ahp-genes associated Mycobacterium tuberculosis resistance to rifampicin (RIF) and isoniazid (INH) in Kyrgyz Republic. We studied 633 smear samples from the primary pulmonary tuberculosis (TB) patients. We verified Mycobacterium tubercul...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1168-x
更新日期:2018-03-22 00:00:00
abstract:BACKGROUND:In the genome of H. pylori 26695, 149 proteins containing the CXXC motif characteristic of thioldisulfide oxidoreductases have been identified to date. However, only two of these proteins have a thioredoxin-like fold (i.e., HP0377 and HP0231) and are periplasm-located. We have previously shown that HP0231 is...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0471-z
更新日期:2015-07-04 00:00:00
abstract:BACKGROUND:The phenomenon of misdiagnosing tuberculosis (TB) by laboratory cross-contamination when culturing Mycobacterium tuberculosis (MTB) has been widely reported and it has an obvious clinical, therapeutic and social impact. The final confirmation of a cross-contamination event requires the molecular identificati...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-30
更新日期:2008-02-14 00:00:00
abstract:BACKGROUND:Photorhabdus are Gram negative bacteria that are pathogenic to insect larvae whilst also having a mutualistic interaction with nematodes from the family Heterorhabditis. Iron is an essential nutrient and bacteria have different mechanisms for obtaining both the ferrous (Fe2+) and ferric (Fe3+) forms of this ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-177
更新日期:2010-06-22 00:00:00
abstract:BACKGROUND:Staphylococcus aureus is an opportunistic commensal bacterium that mostly colonizes the skin and soft tissues. The pathogenicity of S. aureus is due to both its ability to resist antibiotics, and the production of toxins. Here, we characterize a group of genes responsible for toxin production and antibiotic ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-188
更新日期:2013-08-08 00:00:00
abstract:BACKGROUND:Over the last years oleaginous yeasts have been studied for several energetic, oleochemical, medical and pharmaceutical purposes. However, only a small number of yeasts are known and have been deeply exploited. The search for new isolates with high oleaginous capacity becomes imperative, as well as the use o...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01742-6
更新日期:2020-03-14 00:00:00
abstract:BACKGROUND:The mce operons play an important role in the entry of M. tuberculosis into macrophages and non-phagocytic cells. Their non-redundant function as well as complex regulation is implied by the phenotype of mce mutants. Recently, mce1 operon was found to extend over 13 genes, fadD5 (Rv0166) being the first gene...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-128
更新日期:2010-04-27 00:00:00
abstract:BACKGROUND:The genome of Arthrobacter sp. strain FB24 contains a chromate resistance determinant (CRD), consisting of a cluster of 8 genes located on a 10.6 kb fragment of a 96 kb plasmid. The CRD includes chrA, which encodes a putative chromate efflux protein, and three genes with amino acid similarities to the amino ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-199
更新日期:2009-09-16 00:00:00
abstract:BACKGROUND:Brucellosis is an important zoonosis caused by the genus Brucella. In addition Brucella represents potential biological warfare agents due to the high contagious rates for humans and animals. Therefore, the strain typing epidemiological tool may be crucial for tracing back source of infection in outbreaks an...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-66
更新日期:2009-04-07 00:00:00
abstract:BACKGROUND:Invasive aspergillosis (IA) is a major cause of infectious morbidity and mortality in immune compromised patients. Studies on the pathogenesis of IA have been limited by the difficulty to monitor disease progression in real-time. For real-time monitoring of the infection, we recently engineered a bioluminesc...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-105
更新日期:2010-04-08 00:00:00
abstract:BACKGROUND:Cystic fibrosis (CF) lung disease has a unique profile of pathogens predominated by Pseudomonas aeruginosa (PsA) and Staphylococcus aureus (SA). These microorganisms must overcome host immune defense to colonize the CF lungs. Polymorphonuclear neutrophils are a major component of the host defense against bac...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-11-112
更新日期:2011-05-20 00:00:00
abstract:BACKGROUND:The naked mole-rat (NMR, Heterocephalus glaber) is being bred as a novel laboratory animal due to its unique biological characteristics, including longevity, cancer resistance, hypoxia tolerance, and pain insensitivity. It is expected that differences exist between the microbiota of wild NMRs and that of NMR...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1226-4
更新日期:2018-08-22 00:00:00
abstract:BACKGROUND:Avian pathogenic Escherichia coli (APEC) and uropathogenic E. coli (UPEC) are the two main subsets of extraintestinal pathogenic E. coli (ExPEC). Both types have multiple iron acquisition systems, including heme and siderophores. Although iron transport systems involved in the pathogenesis of APEC or UPEC ha...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-143
更新日期:2012-07-20 00:00:00
abstract:BACKGROUND:Bacteria adapted to live within animals can protect their hosts against harmful infections. Beyond antagonism with pathogens, a 'defensive' bacterial symbiont could engage in additional interactions with other colonizing micro-organisms. A single bacterium might thus have cascading ecological impacts on the ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01845-0
更新日期:2020-06-15 00:00:00