Abstract:
BACKGROUND:"Candidatus Phytoplasma aurantifolia", is the causative agent of witches' broom disease in Mexican lime trees (Citrus aurantifolia L.), and is responsible for major losses of Mexican lime trees in Southern Iran and Oman. The pathogen is strictly biotrophic, and thus is completely dependent on living host cells for its survival. The molecular basis of compatibility and disease development in this system is poorly understood. Therefore, we have applied a cDNA- amplified fragment length polymorphism (AFLP) approach to analyze gene expression in Mexican lime trees infected by "Ca. Phytoplasma aurantifolia". RESULTS:We carried out cDNA-AFLP analysis on grafted infected Mexican lime trees of the susceptible cultivar at the representative symptoms stage. Selective amplifications with 43 primer combinations allowed the visualisation of 55 transcript-derived fragments that were expressed differentially between infected and non-infected leaves. We sequenced 51 fragments, 36 of which were identified as lime tree transcripts after homology searching. Of the 36 genes, 70.5% were down-regulated during infection and could be classified into various functional groups. We showed that Mexican lime tree genes that were homologous to known resistance genes tended to be repressed in response to infection. These included the genes for modifier of snc1 and autophagy protein 5. Furthermore, down-regulation of genes involved in metabolism, transcription, transport and cytoskeleton was observed, which included the genes for formin, importin β 3, transducin, L-asparaginase, glycerophosphoryl diester phosphodiesterase, and RNA polymerase β. In contrast, genes that encoded a proline-rich protein, ubiquitin-protein ligase, phosphatidyl glycerol specific phospholipase C-like, and serine/threonine-protein kinase were up-regulated during the infection. CONCLUSION:The present study identifies a number of candidate genes that might be involved in the interaction of Mexican lime trees with "Candidatus Phytoplasma aurantifolia". These results should help to elucidate the molecular basis of the infection process and to identify genes that could be targeted to increase plant resistance and inhibit the growth and reproduction of the pathogen.
journal_name
BMC Microbioljournal_title
BMC microbiologyauthors
Zamharir MG,Mardi M,Alavi SM,Hasanzadeh N,Nekouei MK,Zamanizadeh HR,Alizadeh A,Salekdeh GHdoi
10.1186/1471-2180-11-1subject
Has Abstractpub_date
2011-01-01 00:00:00pages
1issn
1471-2180pii
1471-2180-11-1journal_volume
11pub_type
杂志文章相关文献
BMC MICROBIOLOGY文献大全abstract:BACKGROUND:It is well known that expression of certain bacterial genes responds rapidly to such stimuli as exposure to toxic chemicals and physical agents. It is generally believed that the proteins encoded in these genes are important for successful survival of the organism under the hostile conditions. Analogously, t...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-3-16
更新日期:2003-08-12 00:00:00
abstract:BACKGROUND:The KdpD/KdpE two-component system of Escherichia coli regulates expression of the kdpFABC operon encoding the high affinity K+ transport system KdpFABC. The input domain of KdpD comprises a domain that belongs to the family of universal stress proteins (Usp). It has been previously demonstrated that UspC bi...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-133
更新日期:2009-07-09 00:00:00
abstract:BACKGROUND:Recent studies have identified in Mycobacterium avium subsp. paratuberculosis (MAP), already known as a pathogen in ruminants, a potential zoonotic agent of some autoimmune diseases in humans. Therefore, considering the possible risk for public health, it is necessary a thorough understanding of MAP's gene e...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-87
更新日期:2012-05-30 00:00:00
abstract:BACKGROUND:The emergence of antibiotic resistant pathogenic bacteria has reduced our ability to combat infectious diseases. At the same time the numbers of new antibiotics reaching the market have decreased. This situation has created an urgent need to discover novel antibiotic scaffolds. Recently, the application of p...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0696-5
更新日期:2016-05-10 00:00:00
abstract:BACKGROUND:The use of chemical fungicides against fungal pathogens adversely affects soil and plant health thereby resulting in overall environmental hazards. Therefore, biological source for obtaining antifungal agents is considered as an environment-friendly alternative for controlling fungal pathogens. RESULTS:In t...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1440-8
更新日期:2019-04-02 00:00:00
abstract:BACKGROUND:Rhizobium tropici strain PRF 81 (= SEMIA 4080) has been used in commercial inoculants for application to common-bean crops in Brazil since 1998, due to its high efficiency in fixing nitrogen, competitiveness against indigenous rhizobial populations and capacity to adapt to stressful tropical conditions, repr...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-84
更新日期:2012-05-30 00:00:00
abstract:BACKGROUND:Bartonella henselae is a Gram-negative bacterium transmitted to humans by a scratch from cat in the presence of ectoparasites. Humans infected with B. henselae can result in various clinical diseases including local lymphadenopathy and more serious systemic disease such as persistent bacteremia and endocardi...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01777-9
更新日期:2020-04-10 00:00:00
abstract:BACKGROUND:The lung of patients with cystic fibrosis (CF) is particularly sensitive to Pseudomonas aeruginosa. This bacterium plays an important role in the poor outcome of CF patients. During the disease progress, first acquisition of P. aeruginosa is the key-step in the management of CF patients. Quantitative PCR (qP...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-143
更新日期:2013-06-21 00:00:00
abstract:BACKGROUND:This study evaluated how dosing regimen for intramuscularly-administered ampicillin, composition of Escherichia coli strains with regard to ampicillin susceptibility, and excretion of bacteria from the intestine affected the level of resistance among Escherichia coli strains in the intestine of nursery pigs....
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-016-0823-3
更新日期:2016-09-06 00:00:00
abstract:BACKGROUND:Wogonin, a natural flavonoid-like chemical compound, exhibits anti-inflammatory, antitumor, antiviral, neuroprotective, and anxiolytic effects by modulating a variety of cellular signaling pathways including PI3K-Akt, p53, nuclear factor κB (NF-κB), mitogen-activated protein kinase (MAPK) pathways. In this s...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01916-2
更新日期:2020-07-28 00:00:00
abstract:BACKGROUND:Salmonella enterica is a facultative intracellular pathogen that replicates within a membrane-bound compartment termed Salmonella containing vacuole (SCV). The biogenesis of SCV requires Salmonella type III protein secretion/translocation system and their effector proteins which are translocated into host ce...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-10-228
更新日期:2010-08-26 00:00:00
abstract:BACKGROUND:A disrupted cell cycle progression of hepatocytes was reported in chronic hepatitis C virus (HCV) infection, which can contribute significantly in the associated pathogenesis. The present study aimed to further elaborate these disruptions by evaluating the expression of key cell cycle and apoptotic proteins ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-133
更新日期:2008-08-05 00:00:00
abstract:BACKGROUND:The ability of an intracellular pathogen to establish infection depends on the capacity of the organism to survive and replicate inside the host. Mycobacterium fortuitum is a bacteria that contains genes involved in the detoxification of the oxygen reactive species such as those produced by the host during t...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-100
更新日期:2008-06-19 00:00:00
abstract:BACKGROUND:Enterohemorrhagic Escherichia coli (EHEC) colonizes the intestinal epithelium and causes attaching and effacing (A/E) lesions. Expression of virulence genes, particularly those from the locus of the enterocyte effacement (LEE) pathogenicity island is required for the formation of a type three secretion syste...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-231
更新日期:2012-10-11 00:00:00
abstract:BACKGROUND:Changes in aboveground plant species diversity as well as variations of environmental conditions such as exposure of ecosystems to elevated concentrations of atmospheric carbon dioxide may lead to changes in metabolic activity, composition and diversity of belowground microbial communities, both bacterial an...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-6-68
更新日期:2006-07-27 00:00:00
abstract:BACKGROUND:The symbiotic bacteria associated with edible fungi are valuable microbial resources worthy of in-depth exploration. It is important to analyze the community structure and succession of symbiotic bacteria in mushrooms. This can assist in the isolation of growth-promoting strains that have an essential relati...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-020-01998-y
更新日期:2020-10-14 00:00:00
abstract:BACKGROUND:A biofilm is a complex community of microorganisms that develop on surfaces in diverse environments. The thickness of the biofilm plays a crucial role in the physiology of the immobilized bacteria. The most cariogenic bacteria, mutans streptococci, are common inhabitants of a dental biofilm community. In thi...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-236
更新日期:2008-12-29 00:00:00
abstract:BACKGROUND:Streptomyces lividans is an appealing host for the production of proteins of biotechnological interest due to its relaxed exogenous DNA restriction system and its ability to secrete proteins directly to the medium through the major Sec or the minor Tat routes. Often, protein secretion displays non-uniform ti...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1591-7
更新日期:2019-10-26 00:00:00
abstract:BACKGROUND:Polycyclic aromatic hydrocarbons (PAHs), widespread pollutants in the marine environment, can produce adverse effects in marine organisms and can be transferred to humans through seafood. Our knowledge of PAH-degrading bacterial populations in the marine environment is still very limited, and mainly originat...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-8-50
更新日期:2008-03-25 00:00:00
abstract:BACKGROUND:Aspergillus fumigatus is a ubiquitous saprotrophic fungus and an opportunistic pathogen of humans and animals. Humans and animals can inhale hundreds of A. fumigatus spores daily. Normally this is harmless for humans, but in case of immunodeficiency, invasive pulmonary aspergillosis (IPA) can develop with a ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1244-2
更新日期:2018-09-17 00:00:00
abstract:BACKGROUND:The emergence of carbapenemase producing bacteria, especially New Delhi metallo-β-lactamase (NDM-1) and its variants, worldwide, has raised amajor public health concern. NDM-1 hydrolyzes a wide range of β-lactam antibiotics, including carbapenems, which are the last resort of antibiotics for the treatment of...
journal_title:BMC microbiology
pub_type: 杂志文章,评审
doi:10.1186/s12866-017-1012-8
更新日期:2017-04-27 00:00:00
abstract:BACKGROUND:Wolbachia, one of the most abundant taxa of intracellular Alphaproteobacteria, is widespread among arthropods and filarial nematodes. The presence of these maternally inherited bacteria is associated with modifications of host fitness, including a variety of reproductive abnormalities, such as cytoplasmic in...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1652-y
更新日期:2019-12-24 00:00:00
abstract:BACKGROUND:HSV-1 is a common pathogen that infects 50-90% of the human population worldwide. HSV-1 causes numerous infection-related diseases, some of which are severely life-threatening. There are antiviral medications with activity against HSV-1. However, with the emergence of drug-resistant mutant strains of HSV-1, ...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-019-1639-8
更新日期:2019-12-07 00:00:00
abstract:BACKGROUND:The sequenced O45:K1:H7 Escherichia coli meningitis strain S88 harbors a large virulence plasmid. To identify possible genetic determinants of pS88 virulence, we examined the transcriptomes of 88 plasmidic ORFs corresponding to known and putative virulence genes, and 35 ORFs of unknown function. RESULTS:Qua...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-115
更新日期:2012-06-21 00:00:00
abstract:BACKGROUND:In the genome of H. pylori 26695, 149 proteins containing the CXXC motif characteristic of thioldisulfide oxidoreductases have been identified to date. However, only two of these proteins have a thioredoxin-like fold (i.e., HP0377 and HP0231) and are periplasm-located. We have previously shown that HP0231 is...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-015-0471-z
更新日期:2015-07-04 00:00:00
abstract:BACKGROUND:The compatible solute trehalose is involved in the osmostress response of Rhizobium etli, the microsymbiont of Phaseolus vulgaris. In this work, we reconstructed trehalose metabolism in R. etli, and investigated its role in cellular adaptation and survival to heat and desiccation stress under free living con...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-12-207
更新日期:2012-09-17 00:00:00
abstract:BACKGROUND:The naked mole-rat (NMR, Heterocephalus glaber) is being bred as a novel laboratory animal due to its unique biological characteristics, including longevity, cancer resistance, hypoxia tolerance, and pain insensitivity. It is expected that differences exist between the microbiota of wild NMRs and that of NMR...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/s12866-018-1226-4
更新日期:2018-08-22 00:00:00
abstract:BACKGROUND:The genus Arsenophonus is a group of symbiotic, mainly insect-associated bacteria with rapidly increasing number of records. It is known from a broad spectrum of hosts and symbiotic relationships varying from parasitic son-killers to coevolving mutualists.The present study extends the currently known diversi...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-143
更新日期:2009-07-20 00:00:00
abstract:BACKGROUND:In humans, the intestinal microbiota plays an important role in the maintenance of host health by providing energy, nutrients, and immunological protection. Applying current molecular methods is necessary to surmount the limitations of classical culturing techniques in order to obtain an accurate description...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-9-123
更新日期:2009-06-09 00:00:00
abstract:BACKGROUND:The resurgence of multi-drug resistant tuberculosis (MDR-TB) and HIV associated tuberculosis (TB) are of serious global concern. To contain this situation, new anti-tuberculosis drugs and reduced treatment regimens are imperative. Recently, a nitroimidazole, PA-824, has been shown to be active against both r...
journal_title:BMC microbiology
pub_type: 杂志文章
doi:10.1186/1471-2180-13-218
更新日期:2013-10-01 00:00:00